Gamma-aminobutyric acid (GABA) and acetylcholine (ACh) alleviate water deficit effects in soybean: From gene expression up to growth performance
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.envexpbot.2020.104303 http://hdl.handle.net/11449/209037 |
Resumo: | Bioregulators act as priming agents under abiotic stress conditions. Bioregulators such as gamma-aminobutyric acid (GABA) and acetylcholine (ACh) affect the efficiency of the antioxidant system and the regulation of the stomatal aperture, respectively. The aim of this study was to verify a possible synergistic effect among these bioregulators as attenuators of the effects of water deficiency in Glycine max. We combined the application of GABA and ACh at 2.0 mM in soybean plants under different water regimes. The factors studied were: 1) application of Gaba and ACh in seeds (S); in leaves (L); in seeds and leaves (SL); control without application (C); and (2) water regimes at 100 % field capacity (FC) and water deficit (WD). When the application of bioregulators were removed from the process, a severe decrease in photosynthesis capacity (93 %) was observed on the sixth day (after withholding water), as well as a higher expression of the genes known to be induced by water deficit. The combination of GABA and ACh applied to seeds and leaves under water deficit resulted in a lower decline in photosynthesis, as well as better water-use efficiency and biomass production. Soybean plants subjected to this treatment also showed lower expression of GmABA2, GmLEA3 and GmP5CS genes, lower proline content and increased activity of SOD, CAT and APX compared to the control treatment. The results indicate that the combined exogenous application of GABA and ACh in soybean plants acted to promote increased tolerance to water deficit, showing their potential for use on agricultural areas which are prone to droughts. |
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Gamma-aminobutyric acid (GABA) and acetylcholine (ACh) alleviate water deficit effects in soybean: From gene expression up to growth performanceAbiotic stressBioregulatorsGlycine maxPrimingWater deficitBioregulators act as priming agents under abiotic stress conditions. Bioregulators such as gamma-aminobutyric acid (GABA) and acetylcholine (ACh) affect the efficiency of the antioxidant system and the regulation of the stomatal aperture, respectively. The aim of this study was to verify a possible synergistic effect among these bioregulators as attenuators of the effects of water deficiency in Glycine max. We combined the application of GABA and ACh at 2.0 mM in soybean plants under different water regimes. The factors studied were: 1) application of Gaba and ACh in seeds (S); in leaves (L); in seeds and leaves (SL); control without application (C); and (2) water regimes at 100 % field capacity (FC) and water deficit (WD). When the application of bioregulators were removed from the process, a severe decrease in photosynthesis capacity (93 %) was observed on the sixth day (after withholding water), as well as a higher expression of the genes known to be induced by water deficit. The combination of GABA and ACh applied to seeds and leaves under water deficit resulted in a lower decline in photosynthesis, as well as better water-use efficiency and biomass production. Soybean plants subjected to this treatment also showed lower expression of GmABA2, GmLEA3 and GmP5CS genes, lower proline content and increased activity of SOD, CAT and APX compared to the control treatment. The results indicate that the combined exogenous application of GABA and ACh in soybean plants acted to promote increased tolerance to water deficit, showing their potential for use on agricultural areas which are prone to droughts.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)PIBICUniv Estadual Paulista, Inst Biociencias, Campus Rio Claro, Rio Claro, SP, BrazilUniv Oeste Paulista, Ctr Estudos Ecofisiol Vegetal Oeste Paulista CEVO, Programa Posgrad Prod Vegetal, Presidente Prudente, BrazilUniv Estadual Campinas, Dept Genet Evolucao & Imunol, Campinas, BrazilUniv Oeste Paulista, Programa Posgrad Prod Vegetal, Presidente Prudente, BrazilUniv Fed Pelotas UFPel, Dept Bot, Campus Univ S-N,Cx Postal 345, Pelotas, RS, BrazilUniv Estadual Paulista, Inst Biociencias, Campus Rio Claro, Rio Claro, SP, BrazilElsevier B.V.Universidade Estadual Paulista (Unesp)Univ Oeste PaulistaUniversidade Estadual de Campinas (UNICAMP)Universidade Federal de Pernambuco (UFPE)Braga-Reis, Inae [UNESP]Neris, Daniel MoreiraRibas, Alessandra FerreiraEsteves Vieira, Luiz GonzagaSouza, Gustavo Maia2021-06-25T11:46:32Z2021-06-25T11:46:32Z2021-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11http://dx.doi.org/10.1016/j.envexpbot.2020.104303Environmental And Experimental Botany. Oxford: Pergamon-elsevier Science Ltd, v. 182, 11 p., 2021.0098-8472http://hdl.handle.net/11449/20903710.1016/j.envexpbot.2020.104303WOS:000600612000008Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental And Experimental Botanyinfo:eu-repo/semantics/openAccess2021-10-23T19:23:29Zoai:repositorio.unesp.br:11449/209037Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:24:28.539199Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Gamma-aminobutyric acid (GABA) and acetylcholine (ACh) alleviate water deficit effects in soybean: From gene expression up to growth performance |
title |
Gamma-aminobutyric acid (GABA) and acetylcholine (ACh) alleviate water deficit effects in soybean: From gene expression up to growth performance |
spellingShingle |
Gamma-aminobutyric acid (GABA) and acetylcholine (ACh) alleviate water deficit effects in soybean: From gene expression up to growth performance Braga-Reis, Inae [UNESP] Abiotic stress Bioregulators Glycine max Priming Water deficit |
title_short |
Gamma-aminobutyric acid (GABA) and acetylcholine (ACh) alleviate water deficit effects in soybean: From gene expression up to growth performance |
title_full |
Gamma-aminobutyric acid (GABA) and acetylcholine (ACh) alleviate water deficit effects in soybean: From gene expression up to growth performance |
title_fullStr |
Gamma-aminobutyric acid (GABA) and acetylcholine (ACh) alleviate water deficit effects in soybean: From gene expression up to growth performance |
title_full_unstemmed |
Gamma-aminobutyric acid (GABA) and acetylcholine (ACh) alleviate water deficit effects in soybean: From gene expression up to growth performance |
title_sort |
Gamma-aminobutyric acid (GABA) and acetylcholine (ACh) alleviate water deficit effects in soybean: From gene expression up to growth performance |
author |
Braga-Reis, Inae [UNESP] |
author_facet |
Braga-Reis, Inae [UNESP] Neris, Daniel Moreira Ribas, Alessandra Ferreira Esteves Vieira, Luiz Gonzaga Souza, Gustavo Maia |
author_role |
author |
author2 |
Neris, Daniel Moreira Ribas, Alessandra Ferreira Esteves Vieira, Luiz Gonzaga Souza, Gustavo Maia |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Oeste Paulista Universidade Estadual de Campinas (UNICAMP) Universidade Federal de Pernambuco (UFPE) |
dc.contributor.author.fl_str_mv |
Braga-Reis, Inae [UNESP] Neris, Daniel Moreira Ribas, Alessandra Ferreira Esteves Vieira, Luiz Gonzaga Souza, Gustavo Maia |
dc.subject.por.fl_str_mv |
Abiotic stress Bioregulators Glycine max Priming Water deficit |
topic |
Abiotic stress Bioregulators Glycine max Priming Water deficit |
description |
Bioregulators act as priming agents under abiotic stress conditions. Bioregulators such as gamma-aminobutyric acid (GABA) and acetylcholine (ACh) affect the efficiency of the antioxidant system and the regulation of the stomatal aperture, respectively. The aim of this study was to verify a possible synergistic effect among these bioregulators as attenuators of the effects of water deficiency in Glycine max. We combined the application of GABA and ACh at 2.0 mM in soybean plants under different water regimes. The factors studied were: 1) application of Gaba and ACh in seeds (S); in leaves (L); in seeds and leaves (SL); control without application (C); and (2) water regimes at 100 % field capacity (FC) and water deficit (WD). When the application of bioregulators were removed from the process, a severe decrease in photosynthesis capacity (93 %) was observed on the sixth day (after withholding water), as well as a higher expression of the genes known to be induced by water deficit. The combination of GABA and ACh applied to seeds and leaves under water deficit resulted in a lower decline in photosynthesis, as well as better water-use efficiency and biomass production. Soybean plants subjected to this treatment also showed lower expression of GmABA2, GmLEA3 and GmP5CS genes, lower proline content and increased activity of SOD, CAT and APX compared to the control treatment. The results indicate that the combined exogenous application of GABA and ACh in soybean plants acted to promote increased tolerance to water deficit, showing their potential for use on agricultural areas which are prone to droughts. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:46:32Z 2021-06-25T11:46:32Z 2021-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.envexpbot.2020.104303 Environmental And Experimental Botany. Oxford: Pergamon-elsevier Science Ltd, v. 182, 11 p., 2021. 0098-8472 http://hdl.handle.net/11449/209037 10.1016/j.envexpbot.2020.104303 WOS:000600612000008 |
url |
http://dx.doi.org/10.1016/j.envexpbot.2020.104303 http://hdl.handle.net/11449/209037 |
identifier_str_mv |
Environmental And Experimental Botany. Oxford: Pergamon-elsevier Science Ltd, v. 182, 11 p., 2021. 0098-8472 10.1016/j.envexpbot.2020.104303 WOS:000600612000008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Environmental And Experimental Botany |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129063995310080 |