Loss of Parkin Results in Altered Muscle Stem Cell Differentiation during Regeneration

Detalhes bibliográficos
Autor(a) principal: Esteca, Marcos
Data de Publicação: 2020
Outros Autores: Severino, Matheus B., Silvestre, Joao G., Santos, Gustavo Palmeira dos, Tamborlin, Leticia [UNESP], Luchessi, Augusto D. [UNESP], Moriscot, Anselmo S., Gustafsson, Asa B., Baptista, Igor L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/ijms21218007
http://hdl.handle.net/11449/208937
Resumo: The high capacity of the skeletal muscle to regenerate is due to the presence of muscle stem cells (MuSCs, or satellite cells). The E3 ubiquitin ligase Parkin is a key regulator of mitophagy and is recruited to mitochondria during differentiation of mouse myoblast cell line. However, the function of mitophagy during regeneration has not been investigated in vivo. Here, we have utilized Parkin deficient (Parkin(-/-)) mice to investigate the role of Parkin in skeletal muscle regeneration. We found a persistent deficiency in skeletal muscle regeneration in Parkin(-/-) mice after cardiotoxin (CTX) injury with increased area of fibrosis and decreased cross-sectional area (CSA) of myofibres post-injury. There was also a significant modulation of MuSCs differentiation and mitophagic markers, with altered mitochondrial proteins during skeletal muscle regeneration in Parkin(-/-) mice. Our data suggest that Parkin-mediated mitophagy plays a key role in skeletal muscle regeneration and is necessary for MuSCs differentiation.
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spelling Loss of Parkin Results in Altered Muscle Stem Cell Differentiation during Regenerationsatellite cellsdifferentiationmitochondriamitophagyThe high capacity of the skeletal muscle to regenerate is due to the presence of muscle stem cells (MuSCs, or satellite cells). The E3 ubiquitin ligase Parkin is a key regulator of mitophagy and is recruited to mitochondria during differentiation of mouse myoblast cell line. However, the function of mitophagy during regeneration has not been investigated in vivo. Here, we have utilized Parkin deficient (Parkin(-/-)) mice to investigate the role of Parkin in skeletal muscle regeneration. We found a persistent deficiency in skeletal muscle regeneration in Parkin(-/-) mice after cardiotoxin (CTX) injury with increased area of fibrosis and decreased cross-sectional area (CSA) of myofibres post-injury. There was also a significant modulation of MuSCs differentiation and mitophagic markers, with altered mitochondrial proteins during skeletal muscle regeneration in Parkin(-/-) mice. Our data suggest that Parkin-mediated mitophagy plays a key role in skeletal muscle regeneration and is necessary for MuSCs differentiation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAEPEX-UnicampUniv Estadual Campinas, Sch Appl Sci, Lab Cell & Tissue Biol, BR-13484350 Limeira, BrazilUniv Sao Paulo, Inst Biomed Sci, Dept Anat, BR-05508900 Sao Paulo, BrazilUniv Estadual Campinas, Sch Appl Sci, Lab Biotechnol, BR-13484350 Limeira, BrazilSao Paulo State Univ, Inst Biosci, BR-13506900 Rio Claro, BrazilUniv Calif San Diego, Skaggs Sch Pharm & Pharmacol Sci, La Jolla, CA 92093 USAUniv Estadual Campinas, Sch Appl Sci, Rua Pedro Zaccaria 1300, BR-13484350 Limeira, BrazilSao Paulo State Univ, Inst Biosci, BR-13506900 Rio Claro, BrazilFAPESP: 2016/25876-4FAPESP: 2019/016675FAPESP: 19/12236-5CAPES: 001CNPq: 420265/2018-0FAEPEX-Unicamp: 3269/16FAEPEX-Unicamp: 2155/18FAEPEX-Unicamp: 2486/19MdpiUniversidade Estadual de Campinas (UNICAMP)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Univ Calif San DiegoEsteca, MarcosSeverino, Matheus B.Silvestre, Joao G.Santos, Gustavo Palmeira dosTamborlin, Leticia [UNESP]Luchessi, Augusto D. [UNESP]Moriscot, Anselmo S.Gustafsson, Asa B.Baptista, Igor L.2021-06-25T11:43:42Z2021-06-25T11:43:42Z2020-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article19http://dx.doi.org/10.3390/ijms21218007International Journal Of Molecular Sciences. Basel: Mdpi, v. 21, n. 21, 19 p., 2020.http://hdl.handle.net/11449/20893710.3390/ijms21218007WOS:000589141400001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal Of Molecular Sciencesinfo:eu-repo/semantics/openAccess2021-10-23T19:23:25Zoai:repositorio.unesp.br:11449/208937Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:51:14.442668Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Loss of Parkin Results in Altered Muscle Stem Cell Differentiation during Regeneration
title Loss of Parkin Results in Altered Muscle Stem Cell Differentiation during Regeneration
spellingShingle Loss of Parkin Results in Altered Muscle Stem Cell Differentiation during Regeneration
Esteca, Marcos
satellite cells
differentiation
mitochondria
mitophagy
title_short Loss of Parkin Results in Altered Muscle Stem Cell Differentiation during Regeneration
title_full Loss of Parkin Results in Altered Muscle Stem Cell Differentiation during Regeneration
title_fullStr Loss of Parkin Results in Altered Muscle Stem Cell Differentiation during Regeneration
title_full_unstemmed Loss of Parkin Results in Altered Muscle Stem Cell Differentiation during Regeneration
title_sort Loss of Parkin Results in Altered Muscle Stem Cell Differentiation during Regeneration
author Esteca, Marcos
author_facet Esteca, Marcos
Severino, Matheus B.
Silvestre, Joao G.
Santos, Gustavo Palmeira dos
Tamborlin, Leticia [UNESP]
Luchessi, Augusto D. [UNESP]
Moriscot, Anselmo S.
Gustafsson, Asa B.
Baptista, Igor L.
author_role author
author2 Severino, Matheus B.
Silvestre, Joao G.
Santos, Gustavo Palmeira dos
Tamborlin, Leticia [UNESP]
Luchessi, Augusto D. [UNESP]
Moriscot, Anselmo S.
Gustafsson, Asa B.
Baptista, Igor L.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
Univ Calif San Diego
dc.contributor.author.fl_str_mv Esteca, Marcos
Severino, Matheus B.
Silvestre, Joao G.
Santos, Gustavo Palmeira dos
Tamborlin, Leticia [UNESP]
Luchessi, Augusto D. [UNESP]
Moriscot, Anselmo S.
Gustafsson, Asa B.
Baptista, Igor L.
dc.subject.por.fl_str_mv satellite cells
differentiation
mitochondria
mitophagy
topic satellite cells
differentiation
mitochondria
mitophagy
description The high capacity of the skeletal muscle to regenerate is due to the presence of muscle stem cells (MuSCs, or satellite cells). The E3 ubiquitin ligase Parkin is a key regulator of mitophagy and is recruited to mitochondria during differentiation of mouse myoblast cell line. However, the function of mitophagy during regeneration has not been investigated in vivo. Here, we have utilized Parkin deficient (Parkin(-/-)) mice to investigate the role of Parkin in skeletal muscle regeneration. We found a persistent deficiency in skeletal muscle regeneration in Parkin(-/-) mice after cardiotoxin (CTX) injury with increased area of fibrosis and decreased cross-sectional area (CSA) of myofibres post-injury. There was also a significant modulation of MuSCs differentiation and mitophagic markers, with altered mitochondrial proteins during skeletal muscle regeneration in Parkin(-/-) mice. Our data suggest that Parkin-mediated mitophagy plays a key role in skeletal muscle regeneration and is necessary for MuSCs differentiation.
publishDate 2020
dc.date.none.fl_str_mv 2020-11-01
2021-06-25T11:43:42Z
2021-06-25T11:43:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/ijms21218007
International Journal Of Molecular Sciences. Basel: Mdpi, v. 21, n. 21, 19 p., 2020.
http://hdl.handle.net/11449/208937
10.3390/ijms21218007
WOS:000589141400001
url http://dx.doi.org/10.3390/ijms21218007
http://hdl.handle.net/11449/208937
identifier_str_mv International Journal Of Molecular Sciences. Basel: Mdpi, v. 21, n. 21, 19 p., 2020.
10.3390/ijms21218007
WOS:000589141400001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Journal Of Molecular Sciences
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 19
dc.publisher.none.fl_str_mv Mdpi
publisher.none.fl_str_mv Mdpi
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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