Loss of Parkin Results in Altered Muscle Stem Cell Differentiation during Regeneration
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ijms21218007 http://hdl.handle.net/11449/208937 |
Resumo: | The high capacity of the skeletal muscle to regenerate is due to the presence of muscle stem cells (MuSCs, or satellite cells). The E3 ubiquitin ligase Parkin is a key regulator of mitophagy and is recruited to mitochondria during differentiation of mouse myoblast cell line. However, the function of mitophagy during regeneration has not been investigated in vivo. Here, we have utilized Parkin deficient (Parkin(-/-)) mice to investigate the role of Parkin in skeletal muscle regeneration. We found a persistent deficiency in skeletal muscle regeneration in Parkin(-/-) mice after cardiotoxin (CTX) injury with increased area of fibrosis and decreased cross-sectional area (CSA) of myofibres post-injury. There was also a significant modulation of MuSCs differentiation and mitophagic markers, with altered mitochondrial proteins during skeletal muscle regeneration in Parkin(-/-) mice. Our data suggest that Parkin-mediated mitophagy plays a key role in skeletal muscle regeneration and is necessary for MuSCs differentiation. |
id |
UNSP_b050b06bf2b284e0eb6fb8b13f0fa574 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/208937 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Loss of Parkin Results in Altered Muscle Stem Cell Differentiation during Regenerationsatellite cellsdifferentiationmitochondriamitophagyThe high capacity of the skeletal muscle to regenerate is due to the presence of muscle stem cells (MuSCs, or satellite cells). The E3 ubiquitin ligase Parkin is a key regulator of mitophagy and is recruited to mitochondria during differentiation of mouse myoblast cell line. However, the function of mitophagy during regeneration has not been investigated in vivo. Here, we have utilized Parkin deficient (Parkin(-/-)) mice to investigate the role of Parkin in skeletal muscle regeneration. We found a persistent deficiency in skeletal muscle regeneration in Parkin(-/-) mice after cardiotoxin (CTX) injury with increased area of fibrosis and decreased cross-sectional area (CSA) of myofibres post-injury. There was also a significant modulation of MuSCs differentiation and mitophagic markers, with altered mitochondrial proteins during skeletal muscle regeneration in Parkin(-/-) mice. Our data suggest that Parkin-mediated mitophagy plays a key role in skeletal muscle regeneration and is necessary for MuSCs differentiation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAEPEX-UnicampUniv Estadual Campinas, Sch Appl Sci, Lab Cell & Tissue Biol, BR-13484350 Limeira, BrazilUniv Sao Paulo, Inst Biomed Sci, Dept Anat, BR-05508900 Sao Paulo, BrazilUniv Estadual Campinas, Sch Appl Sci, Lab Biotechnol, BR-13484350 Limeira, BrazilSao Paulo State Univ, Inst Biosci, BR-13506900 Rio Claro, BrazilUniv Calif San Diego, Skaggs Sch Pharm & Pharmacol Sci, La Jolla, CA 92093 USAUniv Estadual Campinas, Sch Appl Sci, Rua Pedro Zaccaria 1300, BR-13484350 Limeira, BrazilSao Paulo State Univ, Inst Biosci, BR-13506900 Rio Claro, BrazilFAPESP: 2016/25876-4FAPESP: 2019/016675FAPESP: 19/12236-5CAPES: 001CNPq: 420265/2018-0FAEPEX-Unicamp: 3269/16FAEPEX-Unicamp: 2155/18FAEPEX-Unicamp: 2486/19MdpiUniversidade Estadual de Campinas (UNICAMP)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Univ Calif San DiegoEsteca, MarcosSeverino, Matheus B.Silvestre, Joao G.Santos, Gustavo Palmeira dosTamborlin, Leticia [UNESP]Luchessi, Augusto D. [UNESP]Moriscot, Anselmo S.Gustafsson, Asa B.Baptista, Igor L.2021-06-25T11:43:42Z2021-06-25T11:43:42Z2020-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article19http://dx.doi.org/10.3390/ijms21218007International Journal Of Molecular Sciences. Basel: Mdpi, v. 21, n. 21, 19 p., 2020.http://hdl.handle.net/11449/20893710.3390/ijms21218007WOS:000589141400001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal Of Molecular Sciencesinfo:eu-repo/semantics/openAccess2021-10-23T19:23:25Zoai:repositorio.unesp.br:11449/208937Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:51:14.442668Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Loss of Parkin Results in Altered Muscle Stem Cell Differentiation during Regeneration |
title |
Loss of Parkin Results in Altered Muscle Stem Cell Differentiation during Regeneration |
spellingShingle |
Loss of Parkin Results in Altered Muscle Stem Cell Differentiation during Regeneration Esteca, Marcos satellite cells differentiation mitochondria mitophagy |
title_short |
Loss of Parkin Results in Altered Muscle Stem Cell Differentiation during Regeneration |
title_full |
Loss of Parkin Results in Altered Muscle Stem Cell Differentiation during Regeneration |
title_fullStr |
Loss of Parkin Results in Altered Muscle Stem Cell Differentiation during Regeneration |
title_full_unstemmed |
Loss of Parkin Results in Altered Muscle Stem Cell Differentiation during Regeneration |
title_sort |
Loss of Parkin Results in Altered Muscle Stem Cell Differentiation during Regeneration |
author |
Esteca, Marcos |
author_facet |
Esteca, Marcos Severino, Matheus B. Silvestre, Joao G. Santos, Gustavo Palmeira dos Tamborlin, Leticia [UNESP] Luchessi, Augusto D. [UNESP] Moriscot, Anselmo S. Gustafsson, Asa B. Baptista, Igor L. |
author_role |
author |
author2 |
Severino, Matheus B. Silvestre, Joao G. Santos, Gustavo Palmeira dos Tamborlin, Leticia [UNESP] Luchessi, Augusto D. [UNESP] Moriscot, Anselmo S. Gustafsson, Asa B. Baptista, Igor L. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Univ Calif San Diego |
dc.contributor.author.fl_str_mv |
Esteca, Marcos Severino, Matheus B. Silvestre, Joao G. Santos, Gustavo Palmeira dos Tamborlin, Leticia [UNESP] Luchessi, Augusto D. [UNESP] Moriscot, Anselmo S. Gustafsson, Asa B. Baptista, Igor L. |
dc.subject.por.fl_str_mv |
satellite cells differentiation mitochondria mitophagy |
topic |
satellite cells differentiation mitochondria mitophagy |
description |
The high capacity of the skeletal muscle to regenerate is due to the presence of muscle stem cells (MuSCs, or satellite cells). The E3 ubiquitin ligase Parkin is a key regulator of mitophagy and is recruited to mitochondria during differentiation of mouse myoblast cell line. However, the function of mitophagy during regeneration has not been investigated in vivo. Here, we have utilized Parkin deficient (Parkin(-/-)) mice to investigate the role of Parkin in skeletal muscle regeneration. We found a persistent deficiency in skeletal muscle regeneration in Parkin(-/-) mice after cardiotoxin (CTX) injury with increased area of fibrosis and decreased cross-sectional area (CSA) of myofibres post-injury. There was also a significant modulation of MuSCs differentiation and mitophagic markers, with altered mitochondrial proteins during skeletal muscle regeneration in Parkin(-/-) mice. Our data suggest that Parkin-mediated mitophagy plays a key role in skeletal muscle regeneration and is necessary for MuSCs differentiation. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-01 2021-06-25T11:43:42Z 2021-06-25T11:43:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ijms21218007 International Journal Of Molecular Sciences. Basel: Mdpi, v. 21, n. 21, 19 p., 2020. http://hdl.handle.net/11449/208937 10.3390/ijms21218007 WOS:000589141400001 |
url |
http://dx.doi.org/10.3390/ijms21218007 http://hdl.handle.net/11449/208937 |
identifier_str_mv |
International Journal Of Molecular Sciences. Basel: Mdpi, v. 21, n. 21, 19 p., 2020. 10.3390/ijms21218007 WOS:000589141400001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal Of Molecular Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
19 |
dc.publisher.none.fl_str_mv |
Mdpi |
publisher.none.fl_str_mv |
Mdpi |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128709949915136 |