Effect of Nellore (Bos indicus) donor age on in-vitro embryo production and pregnancy rate
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/rda.14164 http://hdl.handle.net/11449/240212 |
Resumo: | The aim of this study was to evaluate the effect of age of Nellore (Bos indicus) donors on the efficiency of in vitro embryo production (IVEP) and pregnancy rate. Thirty-six donors, including 11 female calves (13 ± 0.61 months), 17 prepubertal heifers (25 ± 0.78 months) and 8 cows (83 ± 28 months), were submitted to 3 procedures of ovum pickup (OPU) on random days of the estrous cycle at intervals of 21 days. Caspase-3 and IGFBP2 were quantified in oocytes and blastocysts for the evaluation of oocyte and embryo quality. The produced embryos were vitrified (n = 445) and transferred to synchronized recipients. Cows produced a larger number of follicles (cows: 54.5 ± 6.2; calves: 20.0 ± 0.57; prepubertal heifers: 20.8 ± 0.46), total oocytes (cows: 45.97 ± 7.22; calves: 28.93 ± 6.14; prepubertal heifers: 27.21 ± 4.94) and cleaved oocytes (cows: 21.14 ± 4.22; calves: 13.09 ± 3.72; prepubertal heifers: 12.4 ± 3.19). The cleavage rate was similar between age categories; however, cows tended (p < 0.07) to produce a larger number of blastocysts (9.74 ± 2.26) per OPU than calves (5.57 ± 1.99) and prepubertal heifers tended to have a higher blastocyst yield (35.4%) than calves (27.1%) (p <.07). The expression levels of IGFBP2 and caspase-3 were higher in oocytes derived from calves compared to the other two categories. The pregnancy rate was higher in calves (43.1%) and cows (40.4%) than in prepubertal heifers (33.8%) (p =.03). Despite the larger numbers of follicles and viable oocytes in cows, the blastocyst production results and pregnancy rates obtained indicate that the use of young females as oocyte donors in IVEP is feasible and may contribute to reduce the generation interval. |
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Effect of Nellore (Bos indicus) donor age on in-vitro embryo production and pregnancy ratecaspase-3embryo qualityIGFBP 2IVEPoocyte qualityThe aim of this study was to evaluate the effect of age of Nellore (Bos indicus) donors on the efficiency of in vitro embryo production (IVEP) and pregnancy rate. Thirty-six donors, including 11 female calves (13 ± 0.61 months), 17 prepubertal heifers (25 ± 0.78 months) and 8 cows (83 ± 28 months), were submitted to 3 procedures of ovum pickup (OPU) on random days of the estrous cycle at intervals of 21 days. Caspase-3 and IGFBP2 were quantified in oocytes and blastocysts for the evaluation of oocyte and embryo quality. The produced embryos were vitrified (n = 445) and transferred to synchronized recipients. Cows produced a larger number of follicles (cows: 54.5 ± 6.2; calves: 20.0 ± 0.57; prepubertal heifers: 20.8 ± 0.46), total oocytes (cows: 45.97 ± 7.22; calves: 28.93 ± 6.14; prepubertal heifers: 27.21 ± 4.94) and cleaved oocytes (cows: 21.14 ± 4.22; calves: 13.09 ± 3.72; prepubertal heifers: 12.4 ± 3.19). The cleavage rate was similar between age categories; however, cows tended (p < 0.07) to produce a larger number of blastocysts (9.74 ± 2.26) per OPU than calves (5.57 ± 1.99) and prepubertal heifers tended to have a higher blastocyst yield (35.4%) than calves (27.1%) (p <.07). The expression levels of IGFBP2 and caspase-3 were higher in oocytes derived from calves compared to the other two categories. The pregnancy rate was higher in calves (43.1%) and cows (40.4%) than in prepubertal heifers (33.8%) (p =.03). Despite the larger numbers of follicles and viable oocytes in cows, the blastocyst production results and pregnancy rates obtained indicate that the use of young females as oocyte donors in IVEP is feasible and may contribute to reduce the generation interval.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Institute of Animal Science Beef Cattle Research CenterFaculty of Agricultural and Veterinarian Sciences São Paulo State University (UNESP)Vitrogen-WTAvetAnimal Reproduction Laboratory Embrapa Dairy Cattle Gado de LeiteFaculty of Agricultural and Veterinarian Sciences São Paulo State University (UNESP)Beef Cattle Research CenterUniversidade Estadual Paulista (UNESP)Vitrogen-WTAvetEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Silva, Marina Oliveira deBorges, Marcelo Sant'AnaFernandes, Luana Gomes [UNESP]Rodrigues, Naiara Nantes [UNESP]Watanabe, Yeda FumieJoaquim, Daniel CarlinoOliveira, Clara SladeFeuchard, Viviane Luzia Silva daCyrillo, Joslaine Noely Santos Gonçalves dosMercadante, Maria Eugênia ZerlottiMonteiro, Fabio Morato [UNESP]2023-03-01T20:06:37Z2023-03-01T20:06:37Z2022-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article980-988http://dx.doi.org/10.1111/rda.14164Reproduction in Domestic Animals, v. 57, n. 9, p. 980-988, 2022.1439-05310936-6768http://hdl.handle.net/11449/24021210.1111/rda.141642-s2.0-85131626699Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengReproduction in Domestic Animalsinfo:eu-repo/semantics/openAccess2023-03-01T20:06:37Zoai:repositorio.unesp.br:11449/240212Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T20:06:37Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of Nellore (Bos indicus) donor age on in-vitro embryo production and pregnancy rate |
title |
Effect of Nellore (Bos indicus) donor age on in-vitro embryo production and pregnancy rate |
spellingShingle |
Effect of Nellore (Bos indicus) donor age on in-vitro embryo production and pregnancy rate Silva, Marina Oliveira de caspase-3 embryo quality IGFBP 2 IVEP oocyte quality |
title_short |
Effect of Nellore (Bos indicus) donor age on in-vitro embryo production and pregnancy rate |
title_full |
Effect of Nellore (Bos indicus) donor age on in-vitro embryo production and pregnancy rate |
title_fullStr |
Effect of Nellore (Bos indicus) donor age on in-vitro embryo production and pregnancy rate |
title_full_unstemmed |
Effect of Nellore (Bos indicus) donor age on in-vitro embryo production and pregnancy rate |
title_sort |
Effect of Nellore (Bos indicus) donor age on in-vitro embryo production and pregnancy rate |
author |
Silva, Marina Oliveira de |
author_facet |
Silva, Marina Oliveira de Borges, Marcelo Sant'Ana Fernandes, Luana Gomes [UNESP] Rodrigues, Naiara Nantes [UNESP] Watanabe, Yeda Fumie Joaquim, Daniel Carlino Oliveira, Clara Slade Feuchard, Viviane Luzia Silva da Cyrillo, Joslaine Noely Santos Gonçalves dos Mercadante, Maria Eugênia Zerlotti Monteiro, Fabio Morato [UNESP] |
author_role |
author |
author2 |
Borges, Marcelo Sant'Ana Fernandes, Luana Gomes [UNESP] Rodrigues, Naiara Nantes [UNESP] Watanabe, Yeda Fumie Joaquim, Daniel Carlino Oliveira, Clara Slade Feuchard, Viviane Luzia Silva da Cyrillo, Joslaine Noely Santos Gonçalves dos Mercadante, Maria Eugênia Zerlotti Monteiro, Fabio Morato [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Beef Cattle Research Center Universidade Estadual Paulista (UNESP) Vitrogen-WTAvet Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Silva, Marina Oliveira de Borges, Marcelo Sant'Ana Fernandes, Luana Gomes [UNESP] Rodrigues, Naiara Nantes [UNESP] Watanabe, Yeda Fumie Joaquim, Daniel Carlino Oliveira, Clara Slade Feuchard, Viviane Luzia Silva da Cyrillo, Joslaine Noely Santos Gonçalves dos Mercadante, Maria Eugênia Zerlotti Monteiro, Fabio Morato [UNESP] |
dc.subject.por.fl_str_mv |
caspase-3 embryo quality IGFBP 2 IVEP oocyte quality |
topic |
caspase-3 embryo quality IGFBP 2 IVEP oocyte quality |
description |
The aim of this study was to evaluate the effect of age of Nellore (Bos indicus) donors on the efficiency of in vitro embryo production (IVEP) and pregnancy rate. Thirty-six donors, including 11 female calves (13 ± 0.61 months), 17 prepubertal heifers (25 ± 0.78 months) and 8 cows (83 ± 28 months), were submitted to 3 procedures of ovum pickup (OPU) on random days of the estrous cycle at intervals of 21 days. Caspase-3 and IGFBP2 were quantified in oocytes and blastocysts for the evaluation of oocyte and embryo quality. The produced embryos were vitrified (n = 445) and transferred to synchronized recipients. Cows produced a larger number of follicles (cows: 54.5 ± 6.2; calves: 20.0 ± 0.57; prepubertal heifers: 20.8 ± 0.46), total oocytes (cows: 45.97 ± 7.22; calves: 28.93 ± 6.14; prepubertal heifers: 27.21 ± 4.94) and cleaved oocytes (cows: 21.14 ± 4.22; calves: 13.09 ± 3.72; prepubertal heifers: 12.4 ± 3.19). The cleavage rate was similar between age categories; however, cows tended (p < 0.07) to produce a larger number of blastocysts (9.74 ± 2.26) per OPU than calves (5.57 ± 1.99) and prepubertal heifers tended to have a higher blastocyst yield (35.4%) than calves (27.1%) (p <.07). The expression levels of IGFBP2 and caspase-3 were higher in oocytes derived from calves compared to the other two categories. The pregnancy rate was higher in calves (43.1%) and cows (40.4%) than in prepubertal heifers (33.8%) (p =.03). Despite the larger numbers of follicles and viable oocytes in cows, the blastocyst production results and pregnancy rates obtained indicate that the use of young females as oocyte donors in IVEP is feasible and may contribute to reduce the generation interval. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-09-01 2023-03-01T20:06:37Z 2023-03-01T20:06:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/rda.14164 Reproduction in Domestic Animals, v. 57, n. 9, p. 980-988, 2022. 1439-0531 0936-6768 http://hdl.handle.net/11449/240212 10.1111/rda.14164 2-s2.0-85131626699 |
url |
http://dx.doi.org/10.1111/rda.14164 http://hdl.handle.net/11449/240212 |
identifier_str_mv |
Reproduction in Domestic Animals, v. 57, n. 9, p. 980-988, 2022. 1439-0531 0936-6768 10.1111/rda.14164 2-s2.0-85131626699 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Reproduction in Domestic Animals |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
980-988 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046424011603968 |