Determination of Sudan II dye in ethanol fuel by chromatographic and electroanalytical methods

Detalhes bibliográficos
Autor(a) principal: Trindade, Magno Aparecido Gonçalves
Data de Publicação: 2012
Outros Autores: Romanini, Daiane Cristina [UNESP], Zanoni, Maria Valnice Boldrin [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.brjac.com.br/Revista_BrJAC_8.pdf
http://hdl.handle.net/11449/73973
Resumo: Chromatographic and electroanalytical methods were developed to detect and quantify Sudan II (SD-II) dye in fuel ethanol samples. Sudan II is reduced at +0.50 V vs. Ag/AgCl on a glassy carbon electrode using Britton-Robinson buffer (pH 4.0) and N,N-dimethylformamide (70:30, v/v) + sodium dioctyl sulfosuccinate surfactant as supporting electrolyte, due to the azo group. This is the basis for its determination by square-wave voltammetry (SWV). Using the optimized conditions, it is possible to get a linear calibration curve from 3.00×10-6 to 1.80×10-5 mol L-1 (r = 0.998) with limits of detection (LOD) and quantification (LOQ) of 2.05×10-6 and 6.76×10-6 mol L-1, respectively. In addition, the hydroxyl substituent in the SD-II dye is also oxidized at +0.85 V vs. Ag/AgCl, which was conveniently used for its determination by high-performance liquid chromatography coupled to electrochemical detection (HPLC-ED). Under the optimized condition, the SD-II dye was eluted and separated using a reversed-phase column (cyanopropyl, CN) using isocratic elution with the mobile phase containing acetonitrile and aqueous lithium chloride (5.00×10-4 mol L-1) at 70:30 (v/v) and a flow rate of 1.2 mL min-1. Linear calibration curves were obtained from 3.00×10-7 to 2.00×10-6 mol L-1 (r = 0.999) with LOD and LOQ of 3.10×10-8 and 1.05×10-7 mol L-1, respectively. Both methods were simple, fast and suitable to detect and quantify the dye in fuel ethanol samples at recovery values between 83.0 to 102% (SWV) and 88.0 to 112% (HPLC-ED) with satisfactory precision and accuracy.
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spelling Determination of Sudan II dye in ethanol fuel by chromatographic and electroanalytical methodsDye markerElectroanalysisFuel analysisHPLC-EDSudan IIChromatographic and electroanalytical methods were developed to detect and quantify Sudan II (SD-II) dye in fuel ethanol samples. Sudan II is reduced at +0.50 V vs. Ag/AgCl on a glassy carbon electrode using Britton-Robinson buffer (pH 4.0) and N,N-dimethylformamide (70:30, v/v) + sodium dioctyl sulfosuccinate surfactant as supporting electrolyte, due to the azo group. This is the basis for its determination by square-wave voltammetry (SWV). Using the optimized conditions, it is possible to get a linear calibration curve from 3.00×10-6 to 1.80×10-5 mol L-1 (r = 0.998) with limits of detection (LOD) and quantification (LOQ) of 2.05×10-6 and 6.76×10-6 mol L-1, respectively. In addition, the hydroxyl substituent in the SD-II dye is also oxidized at +0.85 V vs. Ag/AgCl, which was conveniently used for its determination by high-performance liquid chromatography coupled to electrochemical detection (HPLC-ED). Under the optimized condition, the SD-II dye was eluted and separated using a reversed-phase column (cyanopropyl, CN) using isocratic elution with the mobile phase containing acetonitrile and aqueous lithium chloride (5.00×10-4 mol L-1) at 70:30 (v/v) and a flow rate of 1.2 mL min-1. Linear calibration curves were obtained from 3.00×10-7 to 2.00×10-6 mol L-1 (r = 0.999) with LOD and LOQ of 3.10×10-8 and 1.05×10-7 mol L-1, respectively. Both methods were simple, fast and suitable to detect and quantify the dye in fuel ethanol samples at recovery values between 83.0 to 102% (SWV) and 88.0 to 112% (HPLC-ED) with satisfactory precision and accuracy.School of Science and Technology Federal University of Grande Dourados, Rodovia Dourados-Itahum, km 12, Dourados, MS 79804-970São Paulo State University - UNESP Department of Analytical Chemistry Institute of Chemistry, Rua Francisco Degni, s/no, Bairro Quitandinha, Araraquara, SP 14800-900São Paulo State University - UNESP Department of Analytical Chemistry Institute of Chemistry, Rua Francisco Degni, s/no, Bairro Quitandinha, Araraquara, SP 14800-900Federal University of Grande DouradosUniversidade Estadual Paulista (Unesp)Trindade, Magno Aparecido GonçalvesRomanini, Daiane Cristina [UNESP]Zanoni, Maria Valnice Boldrin [UNESP]2014-05-27T11:27:23Z2014-05-27T11:27:23Z2012-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article368-374http://www.brjac.com.br/Revista_BrJAC_8.pdfBrazilian Journal of Analytical Chemistry, v. 2, n. 8, p. 368-374, 2012.2179-34252179-3433http://hdl.handle.net/11449/739732-s2.0-848788471480000-0002-2296-1393Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Analytical Chemistry0,1380,138info:eu-repo/semantics/openAccess2021-10-23T11:48:13Zoai:repositorio.unesp.br:11449/73973Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:25:19.894202Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Determination of Sudan II dye in ethanol fuel by chromatographic and electroanalytical methods
title Determination of Sudan II dye in ethanol fuel by chromatographic and electroanalytical methods
spellingShingle Determination of Sudan II dye in ethanol fuel by chromatographic and electroanalytical methods
Trindade, Magno Aparecido Gonçalves
Dye marker
Electroanalysis
Fuel analysis
HPLC-ED
Sudan II
title_short Determination of Sudan II dye in ethanol fuel by chromatographic and electroanalytical methods
title_full Determination of Sudan II dye in ethanol fuel by chromatographic and electroanalytical methods
title_fullStr Determination of Sudan II dye in ethanol fuel by chromatographic and electroanalytical methods
title_full_unstemmed Determination of Sudan II dye in ethanol fuel by chromatographic and electroanalytical methods
title_sort Determination of Sudan II dye in ethanol fuel by chromatographic and electroanalytical methods
author Trindade, Magno Aparecido Gonçalves
author_facet Trindade, Magno Aparecido Gonçalves
Romanini, Daiane Cristina [UNESP]
Zanoni, Maria Valnice Boldrin [UNESP]
author_role author
author2 Romanini, Daiane Cristina [UNESP]
Zanoni, Maria Valnice Boldrin [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Federal University of Grande Dourados
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Trindade, Magno Aparecido Gonçalves
Romanini, Daiane Cristina [UNESP]
Zanoni, Maria Valnice Boldrin [UNESP]
dc.subject.por.fl_str_mv Dye marker
Electroanalysis
Fuel analysis
HPLC-ED
Sudan II
topic Dye marker
Electroanalysis
Fuel analysis
HPLC-ED
Sudan II
description Chromatographic and electroanalytical methods were developed to detect and quantify Sudan II (SD-II) dye in fuel ethanol samples. Sudan II is reduced at +0.50 V vs. Ag/AgCl on a glassy carbon electrode using Britton-Robinson buffer (pH 4.0) and N,N-dimethylformamide (70:30, v/v) + sodium dioctyl sulfosuccinate surfactant as supporting electrolyte, due to the azo group. This is the basis for its determination by square-wave voltammetry (SWV). Using the optimized conditions, it is possible to get a linear calibration curve from 3.00×10-6 to 1.80×10-5 mol L-1 (r = 0.998) with limits of detection (LOD) and quantification (LOQ) of 2.05×10-6 and 6.76×10-6 mol L-1, respectively. In addition, the hydroxyl substituent in the SD-II dye is also oxidized at +0.85 V vs. Ag/AgCl, which was conveniently used for its determination by high-performance liquid chromatography coupled to electrochemical detection (HPLC-ED). Under the optimized condition, the SD-II dye was eluted and separated using a reversed-phase column (cyanopropyl, CN) using isocratic elution with the mobile phase containing acetonitrile and aqueous lithium chloride (5.00×10-4 mol L-1) at 70:30 (v/v) and a flow rate of 1.2 mL min-1. Linear calibration curves were obtained from 3.00×10-7 to 2.00×10-6 mol L-1 (r = 0.999) with LOD and LOQ of 3.10×10-8 and 1.05×10-7 mol L-1, respectively. Both methods were simple, fast and suitable to detect and quantify the dye in fuel ethanol samples at recovery values between 83.0 to 102% (SWV) and 88.0 to 112% (HPLC-ED) with satisfactory precision and accuracy.
publishDate 2012
dc.date.none.fl_str_mv 2012-12-01
2014-05-27T11:27:23Z
2014-05-27T11:27:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.brjac.com.br/Revista_BrJAC_8.pdf
Brazilian Journal of Analytical Chemistry, v. 2, n. 8, p. 368-374, 2012.
2179-3425
2179-3433
http://hdl.handle.net/11449/73973
2-s2.0-84878847148
0000-0002-2296-1393
url http://www.brjac.com.br/Revista_BrJAC_8.pdf
http://hdl.handle.net/11449/73973
identifier_str_mv Brazilian Journal of Analytical Chemistry, v. 2, n. 8, p. 368-374, 2012.
2179-3425
2179-3433
2-s2.0-84878847148
0000-0002-2296-1393
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Analytical Chemistry
0,138
0,138
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 368-374
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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