Molecular detection of vector-borne agents in wild boars (Sus scrofa) and associated ticks from Brazil, with evidence of putative new genotypes of Ehrlichia, Anaplasma, and haemoplasmas

Detalhes bibliográficos
Autor(a) principal: Santana, Matheus de Souza [UNESP]
Data de Publicação: 2022
Outros Autores: Hoppe, Estevam Guilherme Lux [UNESP], Carraro, Paulo Eduardo [UNESP], Calchi, Ana Cláudia [UNESP], de Oliveira, Laryssa Borges [UNESP], do Amaral, Renan Bressianini [UNESP], Mongruel, Anna Claudia Baumel [UNESP], Machado, Dália Monique Ribeiro [UNESP], Burger, Karina Paes [UNESP], Barros-Batestti, Darci Moraes [UNESP], Machado, Rosangela Zacarias [UNESP], André, Marcos Rogério [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1111/tbed.14632
http://hdl.handle.net/11449/241295
Resumo: The present study aimed to investigate, by molecular techniques, the occurrence of Anaplasmataceae, Bartonellaceae, Rickettsiaceae, Mycoplasmataceae, Coxiellaceae, and Babesiidae/Theileriidae agents in blood samples of free-living wild boars (Sus scrofa) and associated ticks in south-eastern Brazil. For this purpose, 67 blood samples and 265 ticks (264 Amblyomma sculptum and one Amblyomma ovale) were analysed. In the screening for Anaplasmataceae agents by a PCR assay based on the 16S rRNA gene, 5.97% blood samples and 50.54% ticks were positive. In the PCR assay for Ehrlichia spp. based on the dsb gene, 9.24% of ticks were positive. Despite the low occurrence, a possible new 16S rRNA genotype of Anaplasma sp. was detected in a wild boar's blood sample. According to phylogenetic analyses based on the 16S rRNA, gltA, and sodB genes and ITS (23S-5S rRNA) intergenic region, it was found that A. sculptum and A. ovale ticks collected from wild boars carry Ehrlichia genotypes phylogenetically associated with Ehrlichia ewingii, Ehrlichia ruminantium, and new Ehrlichia genotypes previously detected in horses, peccaries, and ticks collected from jaguars. In the screening for haemoplasmas by a qPCR based on the 16S rRNA gene, 88.06% of blood samples and 8.69% of ticks were positive. Mycoplasma suis, Mycoplasma parvum, and a possible new haemoplasma genotype were detected in wild boars in south-eastern Brazil. In the screening for Bartonella spp. using a nuoG-based qPCR assay, 3.8% of tick samples were positive. Phylogenetic inferences positioned four nuoG and one r gltA Bartonella sequences into the same clade as Bartonella machadoae. No blood or tick samples from wild boars showed to be positive in the qPCR for Coxiella burnetii based on the IS1111 gene. On the other hand, only 1.6% of ticks were positive in the nested PCR assay for piroplasmids based on the 18S rRNA gene. A 18S rRNA sequence detected in a pool of A. sculptum nymphs was phylogenetically close to Cytauxzoon felis sequences previously detected in cats from the United States. Rickettsia sp. closely related to Rickettsia bellii was detected in a pool of A. sculptum nymphs. This is the first report of haemoplasmas, B. machadoae, and Cytauxzoon spp. in A. sculptum. Wild boars and associated ticks do not seem to participate in the epidemiological cycle of C. burnetii in the region studied. This invasive mammal species may act as a potential disperser of ticks infected with Ehrlichia spp., Bartonella spp., haemotropic mycoplasmas, and Cytauxzoon, and may bring important epidemiological implications in the transmission of bartonelosis, ehrlichiosis, haemoplasmosis, and cytauxzoonosis to humans and animals, more specifically to horses, rodents, pigs, and cats.
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spelling Molecular detection of vector-borne agents in wild boars (Sus scrofa) and associated ticks from Brazil, with evidence of putative new genotypes of Ehrlichia, Anaplasma, and haemoplasmasAmblyomma sppAnaplasmataceaeBartonella spCoxiella burnetiiCytauxzoon sphaemoplasmasRickettsia spThe present study aimed to investigate, by molecular techniques, the occurrence of Anaplasmataceae, Bartonellaceae, Rickettsiaceae, Mycoplasmataceae, Coxiellaceae, and Babesiidae/Theileriidae agents in blood samples of free-living wild boars (Sus scrofa) and associated ticks in south-eastern Brazil. For this purpose, 67 blood samples and 265 ticks (264 Amblyomma sculptum and one Amblyomma ovale) were analysed. In the screening for Anaplasmataceae agents by a PCR assay based on the 16S rRNA gene, 5.97% blood samples and 50.54% ticks were positive. In the PCR assay for Ehrlichia spp. based on the dsb gene, 9.24% of ticks were positive. Despite the low occurrence, a possible new 16S rRNA genotype of Anaplasma sp. was detected in a wild boar's blood sample. According to phylogenetic analyses based on the 16S rRNA, gltA, and sodB genes and ITS (23S-5S rRNA) intergenic region, it was found that A. sculptum and A. ovale ticks collected from wild boars carry Ehrlichia genotypes phylogenetically associated with Ehrlichia ewingii, Ehrlichia ruminantium, and new Ehrlichia genotypes previously detected in horses, peccaries, and ticks collected from jaguars. In the screening for haemoplasmas by a qPCR based on the 16S rRNA gene, 88.06% of blood samples and 8.69% of ticks were positive. Mycoplasma suis, Mycoplasma parvum, and a possible new haemoplasma genotype were detected in wild boars in south-eastern Brazil. In the screening for Bartonella spp. using a nuoG-based qPCR assay, 3.8% of tick samples were positive. Phylogenetic inferences positioned four nuoG and one r gltA Bartonella sequences into the same clade as Bartonella machadoae. No blood or tick samples from wild boars showed to be positive in the qPCR for Coxiella burnetii based on the IS1111 gene. On the other hand, only 1.6% of ticks were positive in the nested PCR assay for piroplasmids based on the 18S rRNA gene. A 18S rRNA sequence detected in a pool of A. sculptum nymphs was phylogenetically close to Cytauxzoon felis sequences previously detected in cats from the United States. Rickettsia sp. closely related to Rickettsia bellii was detected in a pool of A. sculptum nymphs. This is the first report of haemoplasmas, B. machadoae, and Cytauxzoon spp. in A. sculptum. Wild boars and associated ticks do not seem to participate in the epidemiological cycle of C. burnetii in the region studied. This invasive mammal species may act as a potential disperser of ticks infected with Ehrlichia spp., Bartonella spp., haemotropic mycoplasmas, and Cytauxzoon, and may bring important epidemiological implications in the transmission of bartonelosis, ehrlichiosis, haemoplasmosis, and cytauxzoonosis to humans and animals, more specifically to horses, rodents, pigs, and cats.Department of Pathology Reproduction and One Health Faculty of Agricultural and Veterinary Sciences/University State Paulista (FCAV/UNESP)Department of Pathology Reproduction and One Health Faculty of Agricultural and Veterinary Sciences/University State Paulista (FCAV/UNESP)Universidade Estadual Paulista (UNESP)Santana, Matheus de Souza [UNESP]Hoppe, Estevam Guilherme Lux [UNESP]Carraro, Paulo Eduardo [UNESP]Calchi, Ana Cláudia [UNESP]de Oliveira, Laryssa Borges [UNESP]do Amaral, Renan Bressianini [UNESP]Mongruel, Anna Claudia Baumel [UNESP]Machado, Dália Monique Ribeiro [UNESP]Burger, Karina Paes [UNESP]Barros-Batestti, Darci Moraes [UNESP]Machado, Rosangela Zacarias [UNESP]André, Marcos Rogério [UNESP]2023-03-01T20:55:40Z2023-03-01T20:55:40Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1111/tbed.14632Transboundary and Emerging Diseases.1865-16821865-1674http://hdl.handle.net/11449/24129510.1111/tbed.146322-s2.0-85133673616Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTransboundary and Emerging Diseasesinfo:eu-repo/semantics/openAccess2024-06-07T13:02:38Zoai:repositorio.unesp.br:11449/241295Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:02:04.168216Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Molecular detection of vector-borne agents in wild boars (Sus scrofa) and associated ticks from Brazil, with evidence of putative new genotypes of Ehrlichia, Anaplasma, and haemoplasmas
title Molecular detection of vector-borne agents in wild boars (Sus scrofa) and associated ticks from Brazil, with evidence of putative new genotypes of Ehrlichia, Anaplasma, and haemoplasmas
spellingShingle Molecular detection of vector-borne agents in wild boars (Sus scrofa) and associated ticks from Brazil, with evidence of putative new genotypes of Ehrlichia, Anaplasma, and haemoplasmas
Santana, Matheus de Souza [UNESP]
Amblyomma spp
Anaplasmataceae
Bartonella sp
Coxiella burnetii
Cytauxzoon sp
haemoplasmas
Rickettsia sp
title_short Molecular detection of vector-borne agents in wild boars (Sus scrofa) and associated ticks from Brazil, with evidence of putative new genotypes of Ehrlichia, Anaplasma, and haemoplasmas
title_full Molecular detection of vector-borne agents in wild boars (Sus scrofa) and associated ticks from Brazil, with evidence of putative new genotypes of Ehrlichia, Anaplasma, and haemoplasmas
title_fullStr Molecular detection of vector-borne agents in wild boars (Sus scrofa) and associated ticks from Brazil, with evidence of putative new genotypes of Ehrlichia, Anaplasma, and haemoplasmas
title_full_unstemmed Molecular detection of vector-borne agents in wild boars (Sus scrofa) and associated ticks from Brazil, with evidence of putative new genotypes of Ehrlichia, Anaplasma, and haemoplasmas
title_sort Molecular detection of vector-borne agents in wild boars (Sus scrofa) and associated ticks from Brazil, with evidence of putative new genotypes of Ehrlichia, Anaplasma, and haemoplasmas
author Santana, Matheus de Souza [UNESP]
author_facet Santana, Matheus de Souza [UNESP]
Hoppe, Estevam Guilherme Lux [UNESP]
Carraro, Paulo Eduardo [UNESP]
Calchi, Ana Cláudia [UNESP]
de Oliveira, Laryssa Borges [UNESP]
do Amaral, Renan Bressianini [UNESP]
Mongruel, Anna Claudia Baumel [UNESP]
Machado, Dália Monique Ribeiro [UNESP]
Burger, Karina Paes [UNESP]
Barros-Batestti, Darci Moraes [UNESP]
Machado, Rosangela Zacarias [UNESP]
André, Marcos Rogério [UNESP]
author_role author
author2 Hoppe, Estevam Guilherme Lux [UNESP]
Carraro, Paulo Eduardo [UNESP]
Calchi, Ana Cláudia [UNESP]
de Oliveira, Laryssa Borges [UNESP]
do Amaral, Renan Bressianini [UNESP]
Mongruel, Anna Claudia Baumel [UNESP]
Machado, Dália Monique Ribeiro [UNESP]
Burger, Karina Paes [UNESP]
Barros-Batestti, Darci Moraes [UNESP]
Machado, Rosangela Zacarias [UNESP]
André, Marcos Rogério [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Santana, Matheus de Souza [UNESP]
Hoppe, Estevam Guilherme Lux [UNESP]
Carraro, Paulo Eduardo [UNESP]
Calchi, Ana Cláudia [UNESP]
de Oliveira, Laryssa Borges [UNESP]
do Amaral, Renan Bressianini [UNESP]
Mongruel, Anna Claudia Baumel [UNESP]
Machado, Dália Monique Ribeiro [UNESP]
Burger, Karina Paes [UNESP]
Barros-Batestti, Darci Moraes [UNESP]
Machado, Rosangela Zacarias [UNESP]
André, Marcos Rogério [UNESP]
dc.subject.por.fl_str_mv Amblyomma spp
Anaplasmataceae
Bartonella sp
Coxiella burnetii
Cytauxzoon sp
haemoplasmas
Rickettsia sp
topic Amblyomma spp
Anaplasmataceae
Bartonella sp
Coxiella burnetii
Cytauxzoon sp
haemoplasmas
Rickettsia sp
description The present study aimed to investigate, by molecular techniques, the occurrence of Anaplasmataceae, Bartonellaceae, Rickettsiaceae, Mycoplasmataceae, Coxiellaceae, and Babesiidae/Theileriidae agents in blood samples of free-living wild boars (Sus scrofa) and associated ticks in south-eastern Brazil. For this purpose, 67 blood samples and 265 ticks (264 Amblyomma sculptum and one Amblyomma ovale) were analysed. In the screening for Anaplasmataceae agents by a PCR assay based on the 16S rRNA gene, 5.97% blood samples and 50.54% ticks were positive. In the PCR assay for Ehrlichia spp. based on the dsb gene, 9.24% of ticks were positive. Despite the low occurrence, a possible new 16S rRNA genotype of Anaplasma sp. was detected in a wild boar's blood sample. According to phylogenetic analyses based on the 16S rRNA, gltA, and sodB genes and ITS (23S-5S rRNA) intergenic region, it was found that A. sculptum and A. ovale ticks collected from wild boars carry Ehrlichia genotypes phylogenetically associated with Ehrlichia ewingii, Ehrlichia ruminantium, and new Ehrlichia genotypes previously detected in horses, peccaries, and ticks collected from jaguars. In the screening for haemoplasmas by a qPCR based on the 16S rRNA gene, 88.06% of blood samples and 8.69% of ticks were positive. Mycoplasma suis, Mycoplasma parvum, and a possible new haemoplasma genotype were detected in wild boars in south-eastern Brazil. In the screening for Bartonella spp. using a nuoG-based qPCR assay, 3.8% of tick samples were positive. Phylogenetic inferences positioned four nuoG and one r gltA Bartonella sequences into the same clade as Bartonella machadoae. No blood or tick samples from wild boars showed to be positive in the qPCR for Coxiella burnetii based on the IS1111 gene. On the other hand, only 1.6% of ticks were positive in the nested PCR assay for piroplasmids based on the 18S rRNA gene. A 18S rRNA sequence detected in a pool of A. sculptum nymphs was phylogenetically close to Cytauxzoon felis sequences previously detected in cats from the United States. Rickettsia sp. closely related to Rickettsia bellii was detected in a pool of A. sculptum nymphs. This is the first report of haemoplasmas, B. machadoae, and Cytauxzoon spp. in A. sculptum. Wild boars and associated ticks do not seem to participate in the epidemiological cycle of C. burnetii in the region studied. This invasive mammal species may act as a potential disperser of ticks infected with Ehrlichia spp., Bartonella spp., haemotropic mycoplasmas, and Cytauxzoon, and may bring important epidemiological implications in the transmission of bartonelosis, ehrlichiosis, haemoplasmosis, and cytauxzoonosis to humans and animals, more specifically to horses, rodents, pigs, and cats.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
2023-03-01T20:55:40Z
2023-03-01T20:55:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1111/tbed.14632
Transboundary and Emerging Diseases.
1865-1682
1865-1674
http://hdl.handle.net/11449/241295
10.1111/tbed.14632
2-s2.0-85133673616
url http://dx.doi.org/10.1111/tbed.14632
http://hdl.handle.net/11449/241295
identifier_str_mv Transboundary and Emerging Diseases.
1865-1682
1865-1674
10.1111/tbed.14632
2-s2.0-85133673616
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Transboundary and Emerging Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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