Efeitos biológicos do laser de baixa potência sobre o cultivo de células-tronco mesenquimais provenientes do tecido adiposo e da medula óssea de cães

• Autor(a) principal: Heckler, Marta Cristina Thomas [UNESP]
• Data de Publicação: 2014
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/123665
• topic: Anexina A5; Lasers; Cariotipagem; Citometria de fluxo; Celulas - Proliferação; Células-Tronco; Stem cells

Low power laser (LPL) is widely used in different fields of regenerative medicine and dentistry, where it is used to improve the healing process and is beneficial in a variety of disease processes, which can increase the rate of proliferation of various cell lineages. However, there are few studies evaluating the effects of LPL on canine mesenchymal stem cells (MSC). Thus, the aims of this study are: to characterize canine mesenchymal stem cells derived from adipose tissue (AT-MSC) and bone marrow (BM-MSC), to investigate the effect of low power laser aluminium gallium indium phosphide at densities of 1.1 and 8.7 J/cm2 on cellular viability and proliferation, immunogenicity by MHC-II expression and evaluate the karyotype of the irradiated and non-irradiated cells in order to identify the absence of chromosomal abnormalities with the use of LPL. The study revealed that LPL did not affect cell viability and proliferation, MHC-II expression and karyotype of the samples analyzed between groups. Thus, we can conclude that LPL cause no biological effects to canine AT-MSC and BM-MSC with the 1.1 and 8.7 J/cm2 dosages used. Therefore, other dosages to stimulate proliferation without causing deleterious cellular changes of canine MSC must be investigated