Periapical lesions decrease Akt serine phosphorylation and plasma membrane GLUT4 content in rat skeletal muscle
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00784-015-1664-4 http://hdl.handle.net/11449/172237 |
Resumo: | Objectives: Periapical lesion (PL) promotes insulin resistance; however, the mechanisms underlying this alteration are not fully understood. Therefore, in this study, we aimed to evaluate the Akt serine phosphorylation status and GLUT4 expression levels in the gastrocnemius muscle (GM) of rats with PL. Materials and methods: Male Wistar rats (n = 42) were distributed equally into control (CN) and PL groups. The pulpal tissue of the PL group rats was exposed to the oral environment for 30 days. Thereafter, glucose and insulin levels were assessed, followed by homeostasis model assessment of insulin resistance (HOMA-IR). The Akt serine phosphorylation and GLUT4 levels of microsomal (M) and plasma membrane (PM) fractions were evaluated by western blotting and analyzed statistically. Results: Compared to CN group rats, PL group rats had lower insulin sensitivity (as observed by HOMA-IR), lower Akt serine phosphorylation status after insulin stimulus, and lower GLUT4 levels in the PM fraction. However, the M fraction in the PL group did not differ significantly from that of the CN group. Conclusions: PL decreases insulin sensitivity, Akt phosphorylation, and PM GLUT4 content. Clinical relevance: The present study indicates that preventing endodontic disease can thwart insulin resistance. |
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Periapical lesions decrease Akt serine phosphorylation and plasma membrane GLUT4 content in rat skeletal muscleDiabetes mellitusGlucose transporter type 4Insulin resistancePeriapical lesionsObjectives: Periapical lesion (PL) promotes insulin resistance; however, the mechanisms underlying this alteration are not fully understood. Therefore, in this study, we aimed to evaluate the Akt serine phosphorylation status and GLUT4 expression levels in the gastrocnemius muscle (GM) of rats with PL. Materials and methods: Male Wistar rats (n = 42) were distributed equally into control (CN) and PL groups. The pulpal tissue of the PL group rats was exposed to the oral environment for 30 days. Thereafter, glucose and insulin levels were assessed, followed by homeostasis model assessment of insulin resistance (HOMA-IR). The Akt serine phosphorylation and GLUT4 levels of microsomal (M) and plasma membrane (PM) fractions were evaluated by western blotting and analyzed statistically. Results: Compared to CN group rats, PL group rats had lower insulin sensitivity (as observed by HOMA-IR), lower Akt serine phosphorylation status after insulin stimulus, and lower GLUT4 levels in the PM fraction. However, the M fraction in the PL group did not differ significantly from that of the CN group. Conclusions: PL decreases insulin sensitivity, Akt phosphorylation, and PM GLUT4 content. Clinical relevance: The present study indicates that preventing endodontic disease can thwart insulin resistance.Programa de Pós-graduação Multicêntrico em Ciências Fisiológicas-SBFis Department of Basic Sciences Araçatuba Dental School Univ Estadual Paulista (UNESP), Rod. Marechal Rondom, km 527/528Department of Child and Social Dentistry Araçatuba Dental School Univ Estadual Paulista (UNESP)Endodontics Araçatuba Dental School Univ Estadual Paulista (UNESP)Department of Physiology and Biophysics São Paulo Institute of Biomedical Sciences University of São Paulo (USP), Av. Prof. Lineu Prestes, 2415Programa de Pós-graduação Multicêntrico em Ciências Fisiológicas-SBFis Department of Basic Sciences Araçatuba Dental School Univ Estadual Paulista (UNESP), Rod. Marechal Rondom, km 527/528Department of Child and Social Dentistry Araçatuba Dental School Univ Estadual Paulista (UNESP)Endodontics Araçatuba Dental School Univ Estadual Paulista (UNESP)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Pereira, Renato Felipe [UNESP]de Oliveira da Mota, Max Sander [UNESP]de Lima Coutinho Mattera, Maria Sara [UNESP]Tsosura, Thaís Verônica Saori [UNESP]Chiba, Fernando Yamamoto [UNESP]Garbin, Cléa Adas Saliba [UNESP]Ervolino, Edilson [UNESP]Cintra, Luciano Tavares Angelo [UNESP]Okamoto, Maristela MitikoMachado, Ubiratan FabresSumida, Doris Hissako [UNESP]2018-12-11T16:59:19Z2018-12-11T16:59:19Z2016-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1625-1630application/pdfhttp://dx.doi.org/10.1007/s00784-015-1664-4Clinical Oral Investigations, v. 20, n. 7, p. 1625-1630, 2016.1436-37711432-6981http://hdl.handle.net/11449/17223710.1007/s00784-015-1664-42-s2.0-849481718362-s2.0-84948171836.pdf4408095517346846923574308166736244191585257096860000-0003-4859-05830000-0003-4859-05830000-0001-5069-8812Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigations0,9860,986info:eu-repo/semantics/openAccess2024-01-22T06:23:05Zoai:repositorio.unesp.br:11449/172237Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-22T06:23:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Periapical lesions decrease Akt serine phosphorylation and plasma membrane GLUT4 content in rat skeletal muscle |
title |
Periapical lesions decrease Akt serine phosphorylation and plasma membrane GLUT4 content in rat skeletal muscle |
spellingShingle |
Periapical lesions decrease Akt serine phosphorylation and plasma membrane GLUT4 content in rat skeletal muscle Pereira, Renato Felipe [UNESP] Diabetes mellitus Glucose transporter type 4 Insulin resistance Periapical lesions |
title_short |
Periapical lesions decrease Akt serine phosphorylation and plasma membrane GLUT4 content in rat skeletal muscle |
title_full |
Periapical lesions decrease Akt serine phosphorylation and plasma membrane GLUT4 content in rat skeletal muscle |
title_fullStr |
Periapical lesions decrease Akt serine phosphorylation and plasma membrane GLUT4 content in rat skeletal muscle |
title_full_unstemmed |
Periapical lesions decrease Akt serine phosphorylation and plasma membrane GLUT4 content in rat skeletal muscle |
title_sort |
Periapical lesions decrease Akt serine phosphorylation and plasma membrane GLUT4 content in rat skeletal muscle |
author |
Pereira, Renato Felipe [UNESP] |
author_facet |
Pereira, Renato Felipe [UNESP] de Oliveira da Mota, Max Sander [UNESP] de Lima Coutinho Mattera, Maria Sara [UNESP] Tsosura, Thaís Verônica Saori [UNESP] Chiba, Fernando Yamamoto [UNESP] Garbin, Cléa Adas Saliba [UNESP] Ervolino, Edilson [UNESP] Cintra, Luciano Tavares Angelo [UNESP] Okamoto, Maristela Mitiko Machado, Ubiratan Fabres Sumida, Doris Hissako [UNESP] |
author_role |
author |
author2 |
de Oliveira da Mota, Max Sander [UNESP] de Lima Coutinho Mattera, Maria Sara [UNESP] Tsosura, Thaís Verônica Saori [UNESP] Chiba, Fernando Yamamoto [UNESP] Garbin, Cléa Adas Saliba [UNESP] Ervolino, Edilson [UNESP] Cintra, Luciano Tavares Angelo [UNESP] Okamoto, Maristela Mitiko Machado, Ubiratan Fabres Sumida, Doris Hissako [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Pereira, Renato Felipe [UNESP] de Oliveira da Mota, Max Sander [UNESP] de Lima Coutinho Mattera, Maria Sara [UNESP] Tsosura, Thaís Verônica Saori [UNESP] Chiba, Fernando Yamamoto [UNESP] Garbin, Cléa Adas Saliba [UNESP] Ervolino, Edilson [UNESP] Cintra, Luciano Tavares Angelo [UNESP] Okamoto, Maristela Mitiko Machado, Ubiratan Fabres Sumida, Doris Hissako [UNESP] |
dc.subject.por.fl_str_mv |
Diabetes mellitus Glucose transporter type 4 Insulin resistance Periapical lesions |
topic |
Diabetes mellitus Glucose transporter type 4 Insulin resistance Periapical lesions |
description |
Objectives: Periapical lesion (PL) promotes insulin resistance; however, the mechanisms underlying this alteration are not fully understood. Therefore, in this study, we aimed to evaluate the Akt serine phosphorylation status and GLUT4 expression levels in the gastrocnemius muscle (GM) of rats with PL. Materials and methods: Male Wistar rats (n = 42) were distributed equally into control (CN) and PL groups. The pulpal tissue of the PL group rats was exposed to the oral environment for 30 days. Thereafter, glucose and insulin levels were assessed, followed by homeostasis model assessment of insulin resistance (HOMA-IR). The Akt serine phosphorylation and GLUT4 levels of microsomal (M) and plasma membrane (PM) fractions were evaluated by western blotting and analyzed statistically. Results: Compared to CN group rats, PL group rats had lower insulin sensitivity (as observed by HOMA-IR), lower Akt serine phosphorylation status after insulin stimulus, and lower GLUT4 levels in the PM fraction. However, the M fraction in the PL group did not differ significantly from that of the CN group. Conclusions: PL decreases insulin sensitivity, Akt phosphorylation, and PM GLUT4 content. Clinical relevance: The present study indicates that preventing endodontic disease can thwart insulin resistance. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09-01 2018-12-11T16:59:19Z 2018-12-11T16:59:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-015-1664-4 Clinical Oral Investigations, v. 20, n. 7, p. 1625-1630, 2016. 1436-3771 1432-6981 http://hdl.handle.net/11449/172237 10.1007/s00784-015-1664-4 2-s2.0-84948171836 2-s2.0-84948171836.pdf 4408095517346846 9235743081667362 4419158525709686 0000-0003-4859-0583 0000-0003-4859-0583 0000-0001-5069-8812 |
url |
http://dx.doi.org/10.1007/s00784-015-1664-4 http://hdl.handle.net/11449/172237 |
identifier_str_mv |
Clinical Oral Investigations, v. 20, n. 7, p. 1625-1630, 2016. 1436-3771 1432-6981 10.1007/s00784-015-1664-4 2-s2.0-84948171836 2-s2.0-84948171836.pdf 4408095517346846 9235743081667362 4419158525709686 0000-0003-4859-0583 0000-0001-5069-8812 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations 0,986 0,986 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1625-1630 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965693428367360 |