Survey of begomoviruses and the crinivirus, tomato chlorosis virus, in solanaceous in Southeast/Midwest of Brazil

Detalhes bibliográficos
Autor(a) principal: Mituti, Tatiana
Data de Publicação: 2019
Outros Autores: Moura, Mônika F. [UNESP], Macedo, Mônica A., Silva, Talita N. Z., Pinto, Luiz R., Costa, Hélcio, Krause-Sakate, Renate [UNESP], Inoue-Nagata, Alice K., Nunes, Gabriela G., Lima, Mirtes F., Rezende, Jorge A. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s40858-019-00294-z
http://hdl.handle.net/11449/198064
Resumo: The golden mosaic (begomovirus) and the yellowing (crinivirus) diseases are among the main viral diseases occurring in solanaceous crops in Brazil. A survey of viruses associated with both diseases was conducted on cultivated solanaceous plants from 2013 to 2017 to study their diversity and distribution in the Southeast/Midwest regions of Brazil. Samples from potato, eggplant, sweet pepper and tomato plants were collected in fields of seven Brazilian states (Bahia, Espírito Santo, Goiás, Minas Gerais, Paraná, Rio de Janeiro and São Paulo) and in the Federal District. Total RNA/DNA was extracted and tested by RT-PCR/PCR to detect the crinivirus tomato chlorosis virus (ToCV) and begomoviruses, respectively. Representative amplicons were directly sequenced for virus identification. Out of 343 samples, 54 were positive for ToCV: 38.6% in potato, 0.9% in sweet pepper, and 20.9% in tomato. For begomovirus detection, 234 samples were positive. In potato and sweet pepper plants, only tomato severe rugose virus (ToSRV) was detected, while four begomoviruses were detected in tomato plants. ToSRV was detected in 80.1% of the tomato samples, and was the predominant begomovirus. These results indicate a low diversity of crinivirus and begomovirus species infecting cultivated solanaceous crops in Brazil during the survey period.
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spelling Survey of begomoviruses and the crinivirus, tomato chlorosis virus, in solanaceous in Southeast/Midwest of BrazilBemisia tabaciTomato chlorosis virusTomato severe rugose virusThe golden mosaic (begomovirus) and the yellowing (crinivirus) diseases are among the main viral diseases occurring in solanaceous crops in Brazil. A survey of viruses associated with both diseases was conducted on cultivated solanaceous plants from 2013 to 2017 to study their diversity and distribution in the Southeast/Midwest regions of Brazil. Samples from potato, eggplant, sweet pepper and tomato plants were collected in fields of seven Brazilian states (Bahia, Espírito Santo, Goiás, Minas Gerais, Paraná, Rio de Janeiro and São Paulo) and in the Federal District. Total RNA/DNA was extracted and tested by RT-PCR/PCR to detect the crinivirus tomato chlorosis virus (ToCV) and begomoviruses, respectively. Representative amplicons were directly sequenced for virus identification. Out of 343 samples, 54 were positive for ToCV: 38.6% in potato, 0.9% in sweet pepper, and 20.9% in tomato. For begomovirus detection, 234 samples were positive. In potato and sweet pepper plants, only tomato severe rugose virus (ToSRV) was detected, while four begomoviruses were detected in tomato plants. ToSRV was detected in 80.1% of the tomato samples, and was the predominant begomovirus. These results indicate a low diversity of crinivirus and begomovirus species infecting cultivated solanaceous crops in Brazil during the survey period.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)ESALQ/USPUNESP/FCAINCAPEREmbrapa VegetablesCentro de Ensino Unificado de Brasília – CEUBUNESP/FCAFAPESP: 2012/51771-4Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)INCAPEREmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Centro de Ensino Unificado de Brasília – CEUBMituti, TatianaMoura, Mônika F. [UNESP]Macedo, Mônica A.Silva, Talita N. Z.Pinto, Luiz R.Costa, HélcioKrause-Sakate, Renate [UNESP]Inoue-Nagata, Alice K.Nunes, Gabriela G.Lima, Mirtes F.Rezende, Jorge A. M.2020-12-12T00:58:09Z2020-12-12T00:58:09Z2019-08-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article468-472http://dx.doi.org/10.1007/s40858-019-00294-zTropical Plant Pathology, v. 44, n. 5, p. 468-472, 2019.1983-20521982-5676http://hdl.handle.net/11449/19806410.1007/s40858-019-00294-z2-s2.0-85074210865Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTropical Plant Pathologyinfo:eu-repo/semantics/openAccess2021-10-23T08:18:15Zoai:repositorio.unesp.br:11449/198064Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T08:18:15Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Survey of begomoviruses and the crinivirus, tomato chlorosis virus, in solanaceous in Southeast/Midwest of Brazil
title Survey of begomoviruses and the crinivirus, tomato chlorosis virus, in solanaceous in Southeast/Midwest of Brazil
spellingShingle Survey of begomoviruses and the crinivirus, tomato chlorosis virus, in solanaceous in Southeast/Midwest of Brazil
Mituti, Tatiana
Bemisia tabaci
Tomato chlorosis virus
Tomato severe rugose virus
title_short Survey of begomoviruses and the crinivirus, tomato chlorosis virus, in solanaceous in Southeast/Midwest of Brazil
title_full Survey of begomoviruses and the crinivirus, tomato chlorosis virus, in solanaceous in Southeast/Midwest of Brazil
title_fullStr Survey of begomoviruses and the crinivirus, tomato chlorosis virus, in solanaceous in Southeast/Midwest of Brazil
title_full_unstemmed Survey of begomoviruses and the crinivirus, tomato chlorosis virus, in solanaceous in Southeast/Midwest of Brazil
title_sort Survey of begomoviruses and the crinivirus, tomato chlorosis virus, in solanaceous in Southeast/Midwest of Brazil
author Mituti, Tatiana
author_facet Mituti, Tatiana
Moura, Mônika F. [UNESP]
Macedo, Mônica A.
Silva, Talita N. Z.
Pinto, Luiz R.
Costa, Hélcio
Krause-Sakate, Renate [UNESP]
Inoue-Nagata, Alice K.
Nunes, Gabriela G.
Lima, Mirtes F.
Rezende, Jorge A. M.
author_role author
author2 Moura, Mônika F. [UNESP]
Macedo, Mônica A.
Silva, Talita N. Z.
Pinto, Luiz R.
Costa, Hélcio
Krause-Sakate, Renate [UNESP]
Inoue-Nagata, Alice K.
Nunes, Gabriela G.
Lima, Mirtes F.
Rezende, Jorge A. M.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
INCAPER
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Centro de Ensino Unificado de Brasília – CEUB
dc.contributor.author.fl_str_mv Mituti, Tatiana
Moura, Mônika F. [UNESP]
Macedo, Mônica A.
Silva, Talita N. Z.
Pinto, Luiz R.
Costa, Hélcio
Krause-Sakate, Renate [UNESP]
Inoue-Nagata, Alice K.
Nunes, Gabriela G.
Lima, Mirtes F.
Rezende, Jorge A. M.
dc.subject.por.fl_str_mv Bemisia tabaci
Tomato chlorosis virus
Tomato severe rugose virus
topic Bemisia tabaci
Tomato chlorosis virus
Tomato severe rugose virus
description The golden mosaic (begomovirus) and the yellowing (crinivirus) diseases are among the main viral diseases occurring in solanaceous crops in Brazil. A survey of viruses associated with both diseases was conducted on cultivated solanaceous plants from 2013 to 2017 to study their diversity and distribution in the Southeast/Midwest regions of Brazil. Samples from potato, eggplant, sweet pepper and tomato plants were collected in fields of seven Brazilian states (Bahia, Espírito Santo, Goiás, Minas Gerais, Paraná, Rio de Janeiro and São Paulo) and in the Federal District. Total RNA/DNA was extracted and tested by RT-PCR/PCR to detect the crinivirus tomato chlorosis virus (ToCV) and begomoviruses, respectively. Representative amplicons were directly sequenced for virus identification. Out of 343 samples, 54 were positive for ToCV: 38.6% in potato, 0.9% in sweet pepper, and 20.9% in tomato. For begomovirus detection, 234 samples were positive. In potato and sweet pepper plants, only tomato severe rugose virus (ToSRV) was detected, while four begomoviruses were detected in tomato plants. ToSRV was detected in 80.1% of the tomato samples, and was the predominant begomovirus. These results indicate a low diversity of crinivirus and begomovirus species infecting cultivated solanaceous crops in Brazil during the survey period.
publishDate 2019
dc.date.none.fl_str_mv 2019-08-15
2020-12-12T00:58:09Z
2020-12-12T00:58:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s40858-019-00294-z
Tropical Plant Pathology, v. 44, n. 5, p. 468-472, 2019.
1983-2052
1982-5676
http://hdl.handle.net/11449/198064
10.1007/s40858-019-00294-z
2-s2.0-85074210865
url http://dx.doi.org/10.1007/s40858-019-00294-z
http://hdl.handle.net/11449/198064
identifier_str_mv Tropical Plant Pathology, v. 44, n. 5, p. 468-472, 2019.
1983-2052
1982-5676
10.1007/s40858-019-00294-z
2-s2.0-85074210865
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Tropical Plant Pathology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 468-472
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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