Porcine Primordial Germ Cell-Like Cells Generated from Induced Pluripotent Stem Cells Under Different Culture Conditions

• Autor(a) principal: Pieri, Naira Caroline Godoy
• Data de Publicação: 2021
• Outros Autores: de Souza, Aline Fernanda, Botigelli, Ramon Cesar [UNESP], Pessôa, Laís Vicari de Figueiredo, Recchia, Kaiana, Machado, Lucas Simões, Glória, Mayra Hirakawa, de Castro, Raquel Vasconcelos Guimarães [UNESP], Leal, Diego Feitosa, Fantinato Neto, Paulo, Martins, Simone Maria Massami Kitamura, dos Santos Martins, Daniele, Bressan, Fabiana Fernandes, de Andrade, André Furugen Cesar
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1007/s12015-021-10198-8; http://hdl.handle.net/11449/207956
• topic: PGCs; Porcine; Regenerative medicine; Reproduction; Translational medicine

Culture conditions regulate the process of pluripotency acquisition and self-renewal. This study aimed to analyse the influence of the in vitro environment on the induction of porcine induced pluripotent stem cell (piPSCs) differentiation into primordial germ cell-like cells (pPGCLCs). piPSC culture with different supplementation strategies (LIF, bFGF, or LIF plus bFGF) promoted heterogeneous phenotypic profiles. Continuous bFGF supplementation during piPSCs culture was beneficial to support a pluripotent state and the differentiation of piPSCs into pPGCLCs. The pPGCLCs were positive for the gene and protein expression of pluripotent and germinative markers. This study can provide a suitable in vitro model for use in translational studies and to help answer numerous remaining questions about germ cells. Graphical abstract: [Figure not available: see fulltext.]