Cardiac biomarkers as sensitive tools to evaluate the impact of xenobiotics on amphibians: the effects of anionic surfactant linear alkylbenzene sulfonate (LAS)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.ecoenv.2018.01.022 http://hdl.handle.net/11449/179552 |
Resumo: | Amphibian populations have been experiencing a drastic decline worldwide. Aquatic contaminants are among the main factors responsible for this decline, especially in the aquatic environment. The linear alkylbenzene sulfonate (LAS) is of particular concern, since it represents 84% of the anionic surfactants’ trade. In Brazil, the maximal LAS concentration allowed in fresh waters is 0.5 mg L−1, but its potential harmful effects in amphibians remain unknown. Therefore, this study aimed to analyze the effects of a sublethal concentration of LAS (0.5 mg L−1) for 96 h on sensitive cardiac biomarkers of bullfrog tadpoles, Lithobates catesbeianus (Shaw, 1802). For this, we measured the activity level (AL - % of animals), in situ heart rate (fH - bpm), relative ventricular mass (RVM - % of body mass), in vitro myocardial contractility and cardiac histology of the ventricles. Tadpoles’ AL and fH decreased in LAS group. In contrast, the RVM increased, as a result of a hypertrophy of the myocardium, which was corroborated by the enlargement of the nuclear measures and the increase of myocytes’ diameters. These cellular effects resulted in an elevation of the in vitro contractile force of ventricle strips. Acceleration in the contraction (TPT - ms) also occurred, although no alterations in the time to relaxation (THR -ms) were observed. Therefore, it can be concluded that even when exposed to an environmentally safe concentration, this surfactant promotes several alterations in the cardiac function of bullfrog tadpoles that can impair their development, making them more susceptible to predators and less competitive in terms of reproduction success. Thus, LAS concentrations that are considered safe by Brazilian by regulatory agencies must be revised in order to minimize a drastic impact over amphibian populations. This study demonstrates the relevance of employing cardiac biomarkers at different levels (e.g., morphological, physiological and cellular) to evaluate effects of xenobiotics in tadpoles. |
id |
UNSP_b251fba0f5078b7a35ec2daacd501ac2 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/179552 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Cardiac biomarkers as sensitive tools to evaluate the impact of xenobiotics on amphibians: the effects of anionic surfactant linear alkylbenzene sulfonate (LAS)AnuransCardiac function, ecotoxicologyHeartPollutantAmphibian populations have been experiencing a drastic decline worldwide. Aquatic contaminants are among the main factors responsible for this decline, especially in the aquatic environment. The linear alkylbenzene sulfonate (LAS) is of particular concern, since it represents 84% of the anionic surfactants’ trade. In Brazil, the maximal LAS concentration allowed in fresh waters is 0.5 mg L−1, but its potential harmful effects in amphibians remain unknown. Therefore, this study aimed to analyze the effects of a sublethal concentration of LAS (0.5 mg L−1) for 96 h on sensitive cardiac biomarkers of bullfrog tadpoles, Lithobates catesbeianus (Shaw, 1802). For this, we measured the activity level (AL - % of animals), in situ heart rate (fH - bpm), relative ventricular mass (RVM - % of body mass), in vitro myocardial contractility and cardiac histology of the ventricles. Tadpoles’ AL and fH decreased in LAS group. In contrast, the RVM increased, as a result of a hypertrophy of the myocardium, which was corroborated by the enlargement of the nuclear measures and the increase of myocytes’ diameters. These cellular effects resulted in an elevation of the in vitro contractile force of ventricle strips. Acceleration in the contraction (TPT - ms) also occurred, although no alterations in the time to relaxation (THR -ms) were observed. Therefore, it can be concluded that even when exposed to an environmentally safe concentration, this surfactant promotes several alterations in the cardiac function of bullfrog tadpoles that can impair their development, making them more susceptible to predators and less competitive in terms of reproduction success. Thus, LAS concentrations that are considered safe by Brazilian by regulatory agencies must be revised in order to minimize a drastic impact over amphibian populations. This study demonstrates the relevance of employing cardiac biomarkers at different levels (e.g., morphological, physiological and cellular) to evaluate effects of xenobiotics in tadpoles.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratório de Fisiologia da Conservação Universidade Federal de São Carlos Rodovia João Leme dos Santos (SP-264), Km 110, Bairro do ItingaPrograma de Pós-Graduação em Biotecnologia e Monitoramento Ambiental Universidade Federal de São Carlos Rodovia João Leme dos Santos (SP-264), Km 110, Bairro do ItingaDepartamento de Biologia Universidade Estadual Paulista (UNESP)Núcleo de Pesquisa em Ecotoxicologia de Abelhas Universidade Federal de São Carlos Rodovia João Leme dos Santos (SP-264), Km 110, Bairro do ItingaLaboratório de Biologia Estrutural e Funcional Universidade Federal de São Carlos Rodovia João Leme dos Santos (SP-264), Km 110, Bairro do ItingaLaboratório de Microbiologia Ambiental (LaMA) Universidade Federal de São Carlos Rodovia João Leme dos Santos (SP-264), Km 110, Bairro do ItingaDepartamento de Biologia Universidade Estadual Paulista (UNESP)CNPq: # 165801/2013-1FAPESP: 08/57712-4FAPESP: 2014/00946-4CNPq: 305081/2015-2CNPq: 573921/2008-3Universidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (Unesp)Jones-Costa, MonicaFranco-Belussi, Lilian [UNESP]Vidal, Felipe Augusto PintoGongora, Nathália PenteadoCastanho, Luciano Mendesdos Santos Carvalho, CleoniSilva-Zacarin, Elaine Cristina MathiasAbdalla, Fabio CamargoDuarte, Iolanda Cristina SilveiraOliveira, Classius De [UNESP]de Oliveira, Cristiane Ronchi [UNESP]Salla, Raquel Fernanda2018-12-11T17:35:38Z2018-12-11T17:35:38Z2018-04-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article184-190application/pdfhttp://dx.doi.org/10.1016/j.ecoenv.2018.01.022Ecotoxicology and Environmental Safety, v. 151, p. 184-190.1090-24140147-6513http://hdl.handle.net/11449/17955210.1016/j.ecoenv.2018.01.0222-s2.0-850413804542-s2.0-85041380454.pdf57318566502178590000-0002-2029-7900Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEcotoxicology and Environmental Safety1,2011,201info:eu-repo/semantics/openAccess2023-10-20T06:07:37Zoai:repositorio.unesp.br:11449/179552Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:27:41.309079Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cardiac biomarkers as sensitive tools to evaluate the impact of xenobiotics on amphibians: the effects of anionic surfactant linear alkylbenzene sulfonate (LAS) |
title |
Cardiac biomarkers as sensitive tools to evaluate the impact of xenobiotics on amphibians: the effects of anionic surfactant linear alkylbenzene sulfonate (LAS) |
spellingShingle |
Cardiac biomarkers as sensitive tools to evaluate the impact of xenobiotics on amphibians: the effects of anionic surfactant linear alkylbenzene sulfonate (LAS) Jones-Costa, Monica Anurans Cardiac function, ecotoxicology Heart Pollutant |
title_short |
Cardiac biomarkers as sensitive tools to evaluate the impact of xenobiotics on amphibians: the effects of anionic surfactant linear alkylbenzene sulfonate (LAS) |
title_full |
Cardiac biomarkers as sensitive tools to evaluate the impact of xenobiotics on amphibians: the effects of anionic surfactant linear alkylbenzene sulfonate (LAS) |
title_fullStr |
Cardiac biomarkers as sensitive tools to evaluate the impact of xenobiotics on amphibians: the effects of anionic surfactant linear alkylbenzene sulfonate (LAS) |
title_full_unstemmed |
Cardiac biomarkers as sensitive tools to evaluate the impact of xenobiotics on amphibians: the effects of anionic surfactant linear alkylbenzene sulfonate (LAS) |
title_sort |
Cardiac biomarkers as sensitive tools to evaluate the impact of xenobiotics on amphibians: the effects of anionic surfactant linear alkylbenzene sulfonate (LAS) |
author |
Jones-Costa, Monica |
author_facet |
Jones-Costa, Monica Franco-Belussi, Lilian [UNESP] Vidal, Felipe Augusto Pinto Gongora, Nathália Penteado Castanho, Luciano Mendes dos Santos Carvalho, Cleoni Silva-Zacarin, Elaine Cristina Mathias Abdalla, Fabio Camargo Duarte, Iolanda Cristina Silveira Oliveira, Classius De [UNESP] de Oliveira, Cristiane Ronchi [UNESP] Salla, Raquel Fernanda |
author_role |
author |
author2 |
Franco-Belussi, Lilian [UNESP] Vidal, Felipe Augusto Pinto Gongora, Nathália Penteado Castanho, Luciano Mendes dos Santos Carvalho, Cleoni Silva-Zacarin, Elaine Cristina Mathias Abdalla, Fabio Camargo Duarte, Iolanda Cristina Silveira Oliveira, Classius De [UNESP] de Oliveira, Cristiane Ronchi [UNESP] Salla, Raquel Fernanda |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Carlos (UFSCar) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Jones-Costa, Monica Franco-Belussi, Lilian [UNESP] Vidal, Felipe Augusto Pinto Gongora, Nathália Penteado Castanho, Luciano Mendes dos Santos Carvalho, Cleoni Silva-Zacarin, Elaine Cristina Mathias Abdalla, Fabio Camargo Duarte, Iolanda Cristina Silveira Oliveira, Classius De [UNESP] de Oliveira, Cristiane Ronchi [UNESP] Salla, Raquel Fernanda |
dc.subject.por.fl_str_mv |
Anurans Cardiac function, ecotoxicology Heart Pollutant |
topic |
Anurans Cardiac function, ecotoxicology Heart Pollutant |
description |
Amphibian populations have been experiencing a drastic decline worldwide. Aquatic contaminants are among the main factors responsible for this decline, especially in the aquatic environment. The linear alkylbenzene sulfonate (LAS) is of particular concern, since it represents 84% of the anionic surfactants’ trade. In Brazil, the maximal LAS concentration allowed in fresh waters is 0.5 mg L−1, but its potential harmful effects in amphibians remain unknown. Therefore, this study aimed to analyze the effects of a sublethal concentration of LAS (0.5 mg L−1) for 96 h on sensitive cardiac biomarkers of bullfrog tadpoles, Lithobates catesbeianus (Shaw, 1802). For this, we measured the activity level (AL - % of animals), in situ heart rate (fH - bpm), relative ventricular mass (RVM - % of body mass), in vitro myocardial contractility and cardiac histology of the ventricles. Tadpoles’ AL and fH decreased in LAS group. In contrast, the RVM increased, as a result of a hypertrophy of the myocardium, which was corroborated by the enlargement of the nuclear measures and the increase of myocytes’ diameters. These cellular effects resulted in an elevation of the in vitro contractile force of ventricle strips. Acceleration in the contraction (TPT - ms) also occurred, although no alterations in the time to relaxation (THR -ms) were observed. Therefore, it can be concluded that even when exposed to an environmentally safe concentration, this surfactant promotes several alterations in the cardiac function of bullfrog tadpoles that can impair their development, making them more susceptible to predators and less competitive in terms of reproduction success. Thus, LAS concentrations that are considered safe by Brazilian by regulatory agencies must be revised in order to minimize a drastic impact over amphibian populations. This study demonstrates the relevance of employing cardiac biomarkers at different levels (e.g., morphological, physiological and cellular) to evaluate effects of xenobiotics in tadpoles. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:35:38Z 2018-12-11T17:35:38Z 2018-04-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ecoenv.2018.01.022 Ecotoxicology and Environmental Safety, v. 151, p. 184-190. 1090-2414 0147-6513 http://hdl.handle.net/11449/179552 10.1016/j.ecoenv.2018.01.022 2-s2.0-85041380454 2-s2.0-85041380454.pdf 5731856650217859 0000-0002-2029-7900 |
url |
http://dx.doi.org/10.1016/j.ecoenv.2018.01.022 http://hdl.handle.net/11449/179552 |
identifier_str_mv |
Ecotoxicology and Environmental Safety, v. 151, p. 184-190. 1090-2414 0147-6513 10.1016/j.ecoenv.2018.01.022 2-s2.0-85041380454 2-s2.0-85041380454.pdf 5731856650217859 0000-0002-2029-7900 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ecotoxicology and Environmental Safety 1,201 1,201 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
184-190 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128514694578176 |