Diplopods as Soil Bioindicators of Toxicity after Application of Residues from Sewage Treatment Plants and Ethanol Industry
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1017/S1431927616011739 http://hdl.handle.net/11449/178392 |
Resumo: | Residues like sewage sludge and vinasse have been reused as agricultural fertilizers, but they also present a potential to contaminate soils. Diplopods have been considered excellent bioindicators of soil contamination. In the present study, Rhinocricus padbergi were used to assess toxicity in samples of sewage sludge, biosolids, and sugarcane vinasse. The behavioral analysis, mortality rate, and histological, histochemical, and ultrastructural analyses of the midgut of diplopods were the parameters evaluated. Behaviorally, some diplopods avoided burying themselves after 30 days in soil with biosolid or vinasse. Besides, certain residue combinations were able to cause death of all individuals between 60 and 90 days of exposure. The main tissue responses were significant brush border thickening, induction of epithelial renovation, clustering of hemocytes, accumulation of cytoplasmic granules in hepatic cells, hepatic cells with heteropycnotic nuclei, and cytoplasmic degradation. Alterations were observed at various levels among treatments with different samples and exposure times. Ultrastructural analysis revealed elongation of microvilli coated with a layer of an amorphous substance, resulting in a thicker brush border as observed in the histological analysis. After 30 days of exposure, animals showed an accumulation of spherocrystals in hepatic cells and high absorption of substances, based on the elongation of microvilli. Results obtained in the chemical analysis and the behaviors observed in diplopods suggest that animals processed the residues. Therefore, caution should be exercised in the disposal of these residues in agriculture. |
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Diplopods as Soil Bioindicators of Toxicity after Application of Residues from Sewage Treatment Plants and Ethanol Industryhistopathologymillipedessoil toxicityResidues like sewage sludge and vinasse have been reused as agricultural fertilizers, but they also present a potential to contaminate soils. Diplopods have been considered excellent bioindicators of soil contamination. In the present study, Rhinocricus padbergi were used to assess toxicity in samples of sewage sludge, biosolids, and sugarcane vinasse. The behavioral analysis, mortality rate, and histological, histochemical, and ultrastructural analyses of the midgut of diplopods were the parameters evaluated. Behaviorally, some diplopods avoided burying themselves after 30 days in soil with biosolid or vinasse. Besides, certain residue combinations were able to cause death of all individuals between 60 and 90 days of exposure. The main tissue responses were significant brush border thickening, induction of epithelial renovation, clustering of hemocytes, accumulation of cytoplasmic granules in hepatic cells, hepatic cells with heteropycnotic nuclei, and cytoplasmic degradation. Alterations were observed at various levels among treatments with different samples and exposure times. Ultrastructural analysis revealed elongation of microvilli coated with a layer of an amorphous substance, resulting in a thicker brush border as observed in the histological analysis. After 30 days of exposure, animals showed an accumulation of spherocrystals in hepatic cells and high absorption of substances, based on the elongation of microvilli. Results obtained in the chemical analysis and the behaviors observed in diplopods suggest that animals processed the residues. Therefore, caution should be exercised in the disposal of these residues in agriculture.UNESP (São Paulo State University) Institute of Biosciences Department of Biology, Av. 24-A, n1515Hermínio Ometto University Center (UNIARARAS) Laboratory of Structural Biology, Av. Dr. Maximiliano Baruto, n 500UNESP (São Paulo State University) Institute of Biosciences Department of Biology, Av. 24-A, n1515Universidade Estadual Paulista (Unesp)Laboratory of Structural BiologyChristofoletti, Cintya A. [UNESP]Francisco, Annelise [UNESP]Pedro-Escher, Janaína [UNESP]Gastaldi, Vinícius D. [UNESP]Fontanetti, Carmem S. [UNESP]2018-12-11T17:30:04Z2018-12-11T17:30:04Z2016-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1098-1110application/pdfhttp://dx.doi.org/10.1017/S1431927616011739Microscopy and Microanalysis, v. 22, n. 5, p. 1098-1110, 2016.1435-81151431-9276http://hdl.handle.net/11449/17839210.1017/S14319276160117392-s2.0-849941034792-s2.0-84994103479.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicroscopy and Microanalysis0,2920,292info:eu-repo/semantics/openAccess2023-12-11T06:16:26Zoai:repositorio.unesp.br:11449/178392Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:02:38.430045Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Diplopods as Soil Bioindicators of Toxicity after Application of Residues from Sewage Treatment Plants and Ethanol Industry |
title |
Diplopods as Soil Bioindicators of Toxicity after Application of Residues from Sewage Treatment Plants and Ethanol Industry |
spellingShingle |
Diplopods as Soil Bioindicators of Toxicity after Application of Residues from Sewage Treatment Plants and Ethanol Industry Christofoletti, Cintya A. [UNESP] histopathology millipedes soil toxicity |
title_short |
Diplopods as Soil Bioindicators of Toxicity after Application of Residues from Sewage Treatment Plants and Ethanol Industry |
title_full |
Diplopods as Soil Bioindicators of Toxicity after Application of Residues from Sewage Treatment Plants and Ethanol Industry |
title_fullStr |
Diplopods as Soil Bioindicators of Toxicity after Application of Residues from Sewage Treatment Plants and Ethanol Industry |
title_full_unstemmed |
Diplopods as Soil Bioindicators of Toxicity after Application of Residues from Sewage Treatment Plants and Ethanol Industry |
title_sort |
Diplopods as Soil Bioindicators of Toxicity after Application of Residues from Sewage Treatment Plants and Ethanol Industry |
author |
Christofoletti, Cintya A. [UNESP] |
author_facet |
Christofoletti, Cintya A. [UNESP] Francisco, Annelise [UNESP] Pedro-Escher, Janaína [UNESP] Gastaldi, Vinícius D. [UNESP] Fontanetti, Carmem S. [UNESP] |
author_role |
author |
author2 |
Francisco, Annelise [UNESP] Pedro-Escher, Janaína [UNESP] Gastaldi, Vinícius D. [UNESP] Fontanetti, Carmem S. [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Laboratory of Structural Biology |
dc.contributor.author.fl_str_mv |
Christofoletti, Cintya A. [UNESP] Francisco, Annelise [UNESP] Pedro-Escher, Janaína [UNESP] Gastaldi, Vinícius D. [UNESP] Fontanetti, Carmem S. [UNESP] |
dc.subject.por.fl_str_mv |
histopathology millipedes soil toxicity |
topic |
histopathology millipedes soil toxicity |
description |
Residues like sewage sludge and vinasse have been reused as agricultural fertilizers, but they also present a potential to contaminate soils. Diplopods have been considered excellent bioindicators of soil contamination. In the present study, Rhinocricus padbergi were used to assess toxicity in samples of sewage sludge, biosolids, and sugarcane vinasse. The behavioral analysis, mortality rate, and histological, histochemical, and ultrastructural analyses of the midgut of diplopods were the parameters evaluated. Behaviorally, some diplopods avoided burying themselves after 30 days in soil with biosolid or vinasse. Besides, certain residue combinations were able to cause death of all individuals between 60 and 90 days of exposure. The main tissue responses were significant brush border thickening, induction of epithelial renovation, clustering of hemocytes, accumulation of cytoplasmic granules in hepatic cells, hepatic cells with heteropycnotic nuclei, and cytoplasmic degradation. Alterations were observed at various levels among treatments with different samples and exposure times. Ultrastructural analysis revealed elongation of microvilli coated with a layer of an amorphous substance, resulting in a thicker brush border as observed in the histological analysis. After 30 days of exposure, animals showed an accumulation of spherocrystals in hepatic cells and high absorption of substances, based on the elongation of microvilli. Results obtained in the chemical analysis and the behaviors observed in diplopods suggest that animals processed the residues. Therefore, caution should be exercised in the disposal of these residues in agriculture. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10-01 2018-12-11T17:30:04Z 2018-12-11T17:30:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1017/S1431927616011739 Microscopy and Microanalysis, v. 22, n. 5, p. 1098-1110, 2016. 1435-8115 1431-9276 http://hdl.handle.net/11449/178392 10.1017/S1431927616011739 2-s2.0-84994103479 2-s2.0-84994103479.pdf |
url |
http://dx.doi.org/10.1017/S1431927616011739 http://hdl.handle.net/11449/178392 |
identifier_str_mv |
Microscopy and Microanalysis, v. 22, n. 5, p. 1098-1110, 2016. 1435-8115 1431-9276 10.1017/S1431927616011739 2-s2.0-84994103479 2-s2.0-84994103479.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microscopy and Microanalysis 0,292 0,292 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1098-1110 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129154480078848 |