Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra

Detalhes bibliográficos
Autor(a) principal: Volpato, Rodrigo [UNESP]
Data de Publicação: 2015
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/138389
http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/09-05-2016/000857897.pdf
Resumo: The aim of this study was to evaluate the mechanisms involved in cervical opening in bitches with pyometra and healthy bitches. To this end, immunohistochemical evaluations were conducted in different regions of the cervix, epithelium luminal, glandular and musculene for e-NOS, i-NOS, FAS, FAS-L, PGF2α-R and COX-2 receptors. For this purpose, were used 11 bitches with open pyometra, 11 bitches with close pyometra, 6 healthy bitches on follicular phase and 6 on luteal phase of the estrous cycle. All females were subjected to ovariohysterectomy (OSH) and serum progesterone were performed to confirm the phase of the estrous cycle. The measurements were performed using solid phase commercial kits. For immunostaining evaluation two methods were used. In the first, five fields were evaluated in each of these tissues, where each field received a score from 0 to 4 (0 = 0 to 5% of positive field, 1 = 5 to 25% of positive field, 2 = 25 to 50%, 3 = 50 to 75% of positive field and 4 = 75 to 100% of positive field) being considered positive those stained in brown and blue the negative ones. In the second method the markings intensity was assessed subjectively in weak, moderate and strong, respectively receiving scores 1, 2 and 3 for statistical analysis. Readings were taken at five random fields, that were all homogeneous (more than 80% of the staining with the same intensity). The evaluations were performed using a light microscope at a magnification of 400x. Non-parametric statistical test of Mann-Whitney was performed to compare the presence, concentration of each receiver and the region between the closed and open pyometra groups. Among pyometra, follicular phase and luteal phase groups, Kruskall-Wallis test was used, once the comparison made was between three independent groups. The i-NOS and FAS receptors behave in the same way in bitches with open and closed pyometra, not being involved in cervical opening mechanism in this disease. The ...
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spelling Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometraCervix immunohistochemical evaluation of healthy bitches and bitches diagnosed with pyometraCães - ReproduçãoUtero - InfecçõesPiometraImunohistoquímicaImmunohistochemistryThe aim of this study was to evaluate the mechanisms involved in cervical opening in bitches with pyometra and healthy bitches. To this end, immunohistochemical evaluations were conducted in different regions of the cervix, epithelium luminal, glandular and musculene for e-NOS, i-NOS, FAS, FAS-L, PGF2α-R and COX-2 receptors. For this purpose, were used 11 bitches with open pyometra, 11 bitches with close pyometra, 6 healthy bitches on follicular phase and 6 on luteal phase of the estrous cycle. All females were subjected to ovariohysterectomy (OSH) and serum progesterone were performed to confirm the phase of the estrous cycle. The measurements were performed using solid phase commercial kits. For immunostaining evaluation two methods were used. In the first, five fields were evaluated in each of these tissues, where each field received a score from 0 to 4 (0 = 0 to 5% of positive field, 1 = 5 to 25% of positive field, 2 = 25 to 50%, 3 = 50 to 75% of positive field and 4 = 75 to 100% of positive field) being considered positive those stained in brown and blue the negative ones. In the second method the markings intensity was assessed subjectively in weak, moderate and strong, respectively receiving scores 1, 2 and 3 for statistical analysis. Readings were taken at five random fields, that were all homogeneous (more than 80% of the staining with the same intensity). The evaluations were performed using a light microscope at a magnification of 400x. Non-parametric statistical test of Mann-Whitney was performed to compare the presence, concentration of each receiver and the region between the closed and open pyometra groups. Among pyometra, follicular phase and luteal phase groups, Kruskall-Wallis test was used, once the comparison made was between three independent groups. The i-NOS and FAS receptors behave in the same way in bitches with open and closed pyometra, not being involved in cervical opening mechanism in this disease. The ...O objetivo desse estudo foi avaliar os mecanismos envolvidos na abertura cervical em cadelas saudáveis e com o diagnóstico piometra. Para tanto, foram realizadas avaliações imunoistoquímicas em diferentes regiões da cérvix: epitélio luminal, região glandular e região muscular, para os receptores e-NOS, i-NOS, FAS, FAS-L, PGF2α-R e COX-2. Foram utilizadas 11 cadelas com piometra aberta, 11 cadelas com piometra fechada, 6 cadelas saudáveis em fase folicular e 6 em fase luteal do ciclo estral. Todas as fêmeas foram submetidas a ovariohisterectomia (OSH) e dosagens de progesterona foram realizadas para confirmação da fase do ciclo estral. As dosagens foram realizadas utilizando-se kits comerciais em fase sólida. Para avaliar a imunomarcação foram utilizados dois métodos. No primeiro, em cada um desses tecidos foram avaliados cinco campos, cada campo recebeu um escore de 0 a 4 (sendo 0 = 0 a 5% do campo positivo, 1 = 5 a 25% do campo positivo, 2 = 25 a 50%, 3 = 50 a 75% do campo positivo e 4 = 75 a 100% do campo positivo) sendo considerados positivos aqueles corados em marrom e negativos aqueles corados em azul. No segundo método a intensidade das marcações foi avaliada de forma subjetiva em fraca, moderada e forte, recebendo respectivamente escore 1, 2 e 3 para a análise estatística. As leitura foram realizadas em cinco campos aleatórios, sendo todos os campos homogêneos (mais de 80% da coloração com a mesma intensidade). As avaliações foram realizadas em um microscópio de luz no aumento 400 vezes. Foram realizados os testes estatísticos não-paramétrico de Mann-Whitney para comparar a presença, a concentração de cada receptor e a região entre os grupos piometra fechada e aberta. Entre os grupos piometra, fase luteal e fase folicular, utilizou-se o teste Kruskall-Wallis, pois a comparação foi realizada entre 3 grupos independentes. Os receptores i-NOS e FAS comportam-se da mesma maneira em cadelas com piometra...Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual Paulista (Unesp)Lopes, Maria Denise [UNESP]Universidade Estadual Paulista (Unesp)Volpato, Rodrigo [UNESP]2016-05-17T16:51:04Z2016-05-17T16:51:04Z2015-01-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis149 f.application/pdfVOLPATO, Rodrigo. Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra. 2015. 149 f. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária e Zootecnia, 2015.http://hdl.handle.net/11449/138389000857897http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/09-05-2016/000857897.pdf33004064086P16666129914663018Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2024-09-09T17:26:42Zoai:repositorio.unesp.br:11449/138389Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T17:26:42Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra
Cervix immunohistochemical evaluation of healthy bitches and bitches diagnosed with pyometra
title Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra
spellingShingle Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra
Volpato, Rodrigo [UNESP]
Cães - Reprodução
Utero - Infecções
Piometra
Imunohistoquímica
Immunohistochemistry
title_short Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra
title_full Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra
title_fullStr Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra
title_full_unstemmed Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra
title_sort Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra
author Volpato, Rodrigo [UNESP]
author_facet Volpato, Rodrigo [UNESP]
author_role author
dc.contributor.none.fl_str_mv Lopes, Maria Denise [UNESP]
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Volpato, Rodrigo [UNESP]
dc.subject.por.fl_str_mv Cães - Reprodução
Utero - Infecções
Piometra
Imunohistoquímica
Immunohistochemistry
topic Cães - Reprodução
Utero - Infecções
Piometra
Imunohistoquímica
Immunohistochemistry
description The aim of this study was to evaluate the mechanisms involved in cervical opening in bitches with pyometra and healthy bitches. To this end, immunohistochemical evaluations were conducted in different regions of the cervix, epithelium luminal, glandular and musculene for e-NOS, i-NOS, FAS, FAS-L, PGF2α-R and COX-2 receptors. For this purpose, were used 11 bitches with open pyometra, 11 bitches with close pyometra, 6 healthy bitches on follicular phase and 6 on luteal phase of the estrous cycle. All females were subjected to ovariohysterectomy (OSH) and serum progesterone were performed to confirm the phase of the estrous cycle. The measurements were performed using solid phase commercial kits. For immunostaining evaluation two methods were used. In the first, five fields were evaluated in each of these tissues, where each field received a score from 0 to 4 (0 = 0 to 5% of positive field, 1 = 5 to 25% of positive field, 2 = 25 to 50%, 3 = 50 to 75% of positive field and 4 = 75 to 100% of positive field) being considered positive those stained in brown and blue the negative ones. In the second method the markings intensity was assessed subjectively in weak, moderate and strong, respectively receiving scores 1, 2 and 3 for statistical analysis. Readings were taken at five random fields, that were all homogeneous (more than 80% of the staining with the same intensity). The evaluations were performed using a light microscope at a magnification of 400x. Non-parametric statistical test of Mann-Whitney was performed to compare the presence, concentration of each receiver and the region between the closed and open pyometra groups. Among pyometra, follicular phase and luteal phase groups, Kruskall-Wallis test was used, once the comparison made was between three independent groups. The i-NOS and FAS receptors behave in the same way in bitches with open and closed pyometra, not being involved in cervical opening mechanism in this disease. The ...
publishDate 2015
dc.date.none.fl_str_mv 2015-01-21
2016-05-17T16:51:04Z
2016-05-17T16:51:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv VOLPATO, Rodrigo. Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra. 2015. 149 f. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária e Zootecnia, 2015.
http://hdl.handle.net/11449/138389
000857897
http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/09-05-2016/000857897.pdf
33004064086P1
6666129914663018
identifier_str_mv VOLPATO, Rodrigo. Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra. 2015. 149 f. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária e Zootecnia, 2015.
000857897
33004064086P1
6666129914663018
url http://hdl.handle.net/11449/138389
http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/09-05-2016/000857897.pdf
dc.language.iso.fl_str_mv por
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv 149 f.
application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.source.none.fl_str_mv Aleph
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
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instname_str Universidade Estadual Paulista (UNESP)
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reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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