Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/138389 http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/09-05-2016/000857897.pdf |
Resumo: | The aim of this study was to evaluate the mechanisms involved in cervical opening in bitches with pyometra and healthy bitches. To this end, immunohistochemical evaluations were conducted in different regions of the cervix, epithelium luminal, glandular and musculene for e-NOS, i-NOS, FAS, FAS-L, PGF2α-R and COX-2 receptors. For this purpose, were used 11 bitches with open pyometra, 11 bitches with close pyometra, 6 healthy bitches on follicular phase and 6 on luteal phase of the estrous cycle. All females were subjected to ovariohysterectomy (OSH) and serum progesterone were performed to confirm the phase of the estrous cycle. The measurements were performed using solid phase commercial kits. For immunostaining evaluation two methods were used. In the first, five fields were evaluated in each of these tissues, where each field received a score from 0 to 4 (0 = 0 to 5% of positive field, 1 = 5 to 25% of positive field, 2 = 25 to 50%, 3 = 50 to 75% of positive field and 4 = 75 to 100% of positive field) being considered positive those stained in brown and blue the negative ones. In the second method the markings intensity was assessed subjectively in weak, moderate and strong, respectively receiving scores 1, 2 and 3 for statistical analysis. Readings were taken at five random fields, that were all homogeneous (more than 80% of the staining with the same intensity). The evaluations were performed using a light microscope at a magnification of 400x. Non-parametric statistical test of Mann-Whitney was performed to compare the presence, concentration of each receiver and the region between the closed and open pyometra groups. Among pyometra, follicular phase and luteal phase groups, Kruskall-Wallis test was used, once the comparison made was between three independent groups. The i-NOS and FAS receptors behave in the same way in bitches with open and closed pyometra, not being involved in cervical opening mechanism in this disease. The ... |
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Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometraCervix immunohistochemical evaluation of healthy bitches and bitches diagnosed with pyometraCães - ReproduçãoUtero - InfecçõesPiometraImunohistoquímicaImmunohistochemistryThe aim of this study was to evaluate the mechanisms involved in cervical opening in bitches with pyometra and healthy bitches. To this end, immunohistochemical evaluations were conducted in different regions of the cervix, epithelium luminal, glandular and musculene for e-NOS, i-NOS, FAS, FAS-L, PGF2α-R and COX-2 receptors. For this purpose, were used 11 bitches with open pyometra, 11 bitches with close pyometra, 6 healthy bitches on follicular phase and 6 on luteal phase of the estrous cycle. All females were subjected to ovariohysterectomy (OSH) and serum progesterone were performed to confirm the phase of the estrous cycle. The measurements were performed using solid phase commercial kits. For immunostaining evaluation two methods were used. In the first, five fields were evaluated in each of these tissues, where each field received a score from 0 to 4 (0 = 0 to 5% of positive field, 1 = 5 to 25% of positive field, 2 = 25 to 50%, 3 = 50 to 75% of positive field and 4 = 75 to 100% of positive field) being considered positive those stained in brown and blue the negative ones. In the second method the markings intensity was assessed subjectively in weak, moderate and strong, respectively receiving scores 1, 2 and 3 for statistical analysis. Readings were taken at five random fields, that were all homogeneous (more than 80% of the staining with the same intensity). The evaluations were performed using a light microscope at a magnification of 400x. Non-parametric statistical test of Mann-Whitney was performed to compare the presence, concentration of each receiver and the region between the closed and open pyometra groups. Among pyometra, follicular phase and luteal phase groups, Kruskall-Wallis test was used, once the comparison made was between three independent groups. The i-NOS and FAS receptors behave in the same way in bitches with open and closed pyometra, not being involved in cervical opening mechanism in this disease. The ...O objetivo desse estudo foi avaliar os mecanismos envolvidos na abertura cervical em cadelas saudáveis e com o diagnóstico piometra. Para tanto, foram realizadas avaliações imunoistoquímicas em diferentes regiões da cérvix: epitélio luminal, região glandular e região muscular, para os receptores e-NOS, i-NOS, FAS, FAS-L, PGF2α-R e COX-2. Foram utilizadas 11 cadelas com piometra aberta, 11 cadelas com piometra fechada, 6 cadelas saudáveis em fase folicular e 6 em fase luteal do ciclo estral. Todas as fêmeas foram submetidas a ovariohisterectomia (OSH) e dosagens de progesterona foram realizadas para confirmação da fase do ciclo estral. As dosagens foram realizadas utilizando-se kits comerciais em fase sólida. Para avaliar a imunomarcação foram utilizados dois métodos. No primeiro, em cada um desses tecidos foram avaliados cinco campos, cada campo recebeu um escore de 0 a 4 (sendo 0 = 0 a 5% do campo positivo, 1 = 5 a 25% do campo positivo, 2 = 25 a 50%, 3 = 50 a 75% do campo positivo e 4 = 75 a 100% do campo positivo) sendo considerados positivos aqueles corados em marrom e negativos aqueles corados em azul. No segundo método a intensidade das marcações foi avaliada de forma subjetiva em fraca, moderada e forte, recebendo respectivamente escore 1, 2 e 3 para a análise estatística. As leitura foram realizadas em cinco campos aleatórios, sendo todos os campos homogêneos (mais de 80% da coloração com a mesma intensidade). As avaliações foram realizadas em um microscópio de luz no aumento 400 vezes. Foram realizados os testes estatísticos não-paramétrico de Mann-Whitney para comparar a presença, a concentração de cada receptor e a região entre os grupos piometra fechada e aberta. Entre os grupos piometra, fase luteal e fase folicular, utilizou-se o teste Kruskall-Wallis, pois a comparação foi realizada entre 3 grupos independentes. Os receptores i-NOS e FAS comportam-se da mesma maneira em cadelas com piometra...Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual Paulista (Unesp)Lopes, Maria Denise [UNESP]Universidade Estadual Paulista (Unesp)Volpato, Rodrigo [UNESP]2016-05-17T16:51:04Z2016-05-17T16:51:04Z2015-01-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis149 f.application/pdfVOLPATO, Rodrigo. Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra. 2015. 149 f. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária e Zootecnia, 2015.http://hdl.handle.net/11449/138389000857897http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/09-05-2016/000857897.pdf33004064086P16666129914663018Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2024-09-09T17:26:42Zoai:repositorio.unesp.br:11449/138389Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T17:26:42Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra Cervix immunohistochemical evaluation of healthy bitches and bitches diagnosed with pyometra |
title |
Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra |
spellingShingle |
Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra Volpato, Rodrigo [UNESP] Cães - Reprodução Utero - Infecções Piometra Imunohistoquímica Immunohistochemistry |
title_short |
Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra |
title_full |
Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra |
title_fullStr |
Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra |
title_full_unstemmed |
Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra |
title_sort |
Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra |
author |
Volpato, Rodrigo [UNESP] |
author_facet |
Volpato, Rodrigo [UNESP] |
author_role |
author |
dc.contributor.none.fl_str_mv |
Lopes, Maria Denise [UNESP] Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Volpato, Rodrigo [UNESP] |
dc.subject.por.fl_str_mv |
Cães - Reprodução Utero - Infecções Piometra Imunohistoquímica Immunohistochemistry |
topic |
Cães - Reprodução Utero - Infecções Piometra Imunohistoquímica Immunohistochemistry |
description |
The aim of this study was to evaluate the mechanisms involved in cervical opening in bitches with pyometra and healthy bitches. To this end, immunohistochemical evaluations were conducted in different regions of the cervix, epithelium luminal, glandular and musculene for e-NOS, i-NOS, FAS, FAS-L, PGF2α-R and COX-2 receptors. For this purpose, were used 11 bitches with open pyometra, 11 bitches with close pyometra, 6 healthy bitches on follicular phase and 6 on luteal phase of the estrous cycle. All females were subjected to ovariohysterectomy (OSH) and serum progesterone were performed to confirm the phase of the estrous cycle. The measurements were performed using solid phase commercial kits. For immunostaining evaluation two methods were used. In the first, five fields were evaluated in each of these tissues, where each field received a score from 0 to 4 (0 = 0 to 5% of positive field, 1 = 5 to 25% of positive field, 2 = 25 to 50%, 3 = 50 to 75% of positive field and 4 = 75 to 100% of positive field) being considered positive those stained in brown and blue the negative ones. In the second method the markings intensity was assessed subjectively in weak, moderate and strong, respectively receiving scores 1, 2 and 3 for statistical analysis. Readings were taken at five random fields, that were all homogeneous (more than 80% of the staining with the same intensity). The evaluations were performed using a light microscope at a magnification of 400x. Non-parametric statistical test of Mann-Whitney was performed to compare the presence, concentration of each receiver and the region between the closed and open pyometra groups. Among pyometra, follicular phase and luteal phase groups, Kruskall-Wallis test was used, once the comparison made was between three independent groups. The i-NOS and FAS receptors behave in the same way in bitches with open and closed pyometra, not being involved in cervical opening mechanism in this disease. The ... |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-21 2016-05-17T16:51:04Z 2016-05-17T16:51:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
VOLPATO, Rodrigo. Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra. 2015. 149 f. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária e Zootecnia, 2015. http://hdl.handle.net/11449/138389 000857897 http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/09-05-2016/000857897.pdf 33004064086P1 6666129914663018 |
identifier_str_mv |
VOLPATO, Rodrigo. Avaliação imunoistoquimica da cérvix de cadelas saudáveis e com o diagnóstico de piometra. 2015. 149 f. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária e Zootecnia, 2015. 000857897 33004064086P1 6666129914663018 |
url |
http://hdl.handle.net/11449/138389 http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/09-05-2016/000857897.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
149 f. application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.source.none.fl_str_mv |
Aleph reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546576055894016 |