Avaliação dos produtos de degradação oxidativa da Hb S em eritrócitos de doentes falcêmicos
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1516-84842001000100009 http://hdl.handle.net/11449/66694 |
Resumo: | Analysis of the products of oxidative degradation of Hb S was made by methahemoglobin measurement and a count of red blood cells with Heinz bodies. Free radicals originating from oxidation cause extensive injury to erythrocytes, decreasing their useful survival period especially in Hb S carriers. The Superoxide ion (O 2) is the most responsible for the oxidation process of Hb forming membrane-bound haemachromes which afterwards evolve to Heinz bodies, damaging the membrane and provoking erythrocytes hemolysis. The results from this work showed that the SS genotype is more susceptible to the action of the free radicals than the S/Tal genotype. The β genotype has a lower oxidative susceptibility than the SS because it has only one β s mutation. The results allowed us to conclude that: a) the simple presence of Hb S, independent of its genotype and its concentration, is sufficient to produce methaemoglobin from this Hb; b) there is not a direct relationship between methaetnoglobin concentration and the Heinz bodies count; c) the intensity of Heinz bodies in the sickled erythrocytes seems to be independent of the Hb Fetal concentration; d) The genotype SS is more susceptible to Hb oxidation with the release of products of oxidative degradation; e) methaemoglobin formation in blood of people with Hb AA and Hb AS, assessed over 24, 48, 72 and 163 hourly-periods, showed greater oxidative intensity in the Hb AS compared with Hb AA. |
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Avaliação dos produtos de degradação oxidativa da Hb S em eritrócitos de doentes falcêmicosEvaluation of the oxidative degradation products of Hb S in sickle cell diseaseAnalysis of the products of oxidative degradation of Hb S was made by methahemoglobin measurement and a count of red blood cells with Heinz bodies. Free radicals originating from oxidation cause extensive injury to erythrocytes, decreasing their useful survival period especially in Hb S carriers. The Superoxide ion (O 2) is the most responsible for the oxidation process of Hb forming membrane-bound haemachromes which afterwards evolve to Heinz bodies, damaging the membrane and provoking erythrocytes hemolysis. The results from this work showed that the SS genotype is more susceptible to the action of the free radicals than the S/Tal genotype. The β genotype has a lower oxidative susceptibility than the SS because it has only one β s mutation. The results allowed us to conclude that: a) the simple presence of Hb S, independent of its genotype and its concentration, is sufficient to produce methaemoglobin from this Hb; b) there is not a direct relationship between methaetnoglobin concentration and the Heinz bodies count; c) the intensity of Heinz bodies in the sickled erythrocytes seems to be independent of the Hb Fetal concentration; d) The genotype SS is more susceptible to Hb oxidation with the release of products of oxidative degradation; e) methaemoglobin formation in blood of people with Hb AA and Hb AS, assessed over 24, 48, 72 and 163 hourly-periods, showed greater oxidative intensity in the Hb AS compared with Hb AA.Instituto de Biociências, Letras e Ciências Exatas/UNESP Câmpus de São José do Rio Preto Uniuersidade Estadual Paulista Júlio de Mesquita Filho, SPInstituto de Biociências, Letras e Ciências Exatas/UNESP Câmpus de São José do Rio Preto Uniuersidade Estadual Paulista Júlio de Mesquita Filho, SPUniversidade Estadual Paulista (Unesp)Souza, Patricia C. [UNESP]2014-05-27T11:20:21Z2014-05-27T11:20:21Z2001-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article53-54application/pdfhttp://dx.doi.org/10.1590/S1516-84842001000100009Revista Brasileira de Hematologia e Hemoterapia, v. 23, n. 1, p. 53-54, 2001.1516-8484http://hdl.handle.net/11449/6669410.1590/S1516-84842001000100009S1516-848420010001000092-s2.0-525491155532-s2.0-52549115553.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporRevista Brasileira de Hematologia e Hemoterapia0,335info:eu-repo/semantics/openAccess2023-11-01T06:12:26Zoai:repositorio.unesp.br:11449/66694Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:37:52.625273Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Avaliação dos produtos de degradação oxidativa da Hb S em eritrócitos de doentes falcêmicos Evaluation of the oxidative degradation products of Hb S in sickle cell disease |
title |
Avaliação dos produtos de degradação oxidativa da Hb S em eritrócitos de doentes falcêmicos |
spellingShingle |
Avaliação dos produtos de degradação oxidativa da Hb S em eritrócitos de doentes falcêmicos Souza, Patricia C. [UNESP] |
title_short |
Avaliação dos produtos de degradação oxidativa da Hb S em eritrócitos de doentes falcêmicos |
title_full |
Avaliação dos produtos de degradação oxidativa da Hb S em eritrócitos de doentes falcêmicos |
title_fullStr |
Avaliação dos produtos de degradação oxidativa da Hb S em eritrócitos de doentes falcêmicos |
title_full_unstemmed |
Avaliação dos produtos de degradação oxidativa da Hb S em eritrócitos de doentes falcêmicos |
title_sort |
Avaliação dos produtos de degradação oxidativa da Hb S em eritrócitos de doentes falcêmicos |
author |
Souza, Patricia C. [UNESP] |
author_facet |
Souza, Patricia C. [UNESP] |
author_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Souza, Patricia C. [UNESP] |
description |
Analysis of the products of oxidative degradation of Hb S was made by methahemoglobin measurement and a count of red blood cells with Heinz bodies. Free radicals originating from oxidation cause extensive injury to erythrocytes, decreasing their useful survival period especially in Hb S carriers. The Superoxide ion (O 2) is the most responsible for the oxidation process of Hb forming membrane-bound haemachromes which afterwards evolve to Heinz bodies, damaging the membrane and provoking erythrocytes hemolysis. The results from this work showed that the SS genotype is more susceptible to the action of the free radicals than the S/Tal genotype. The β genotype has a lower oxidative susceptibility than the SS because it has only one β s mutation. The results allowed us to conclude that: a) the simple presence of Hb S, independent of its genotype and its concentration, is sufficient to produce methaemoglobin from this Hb; b) there is not a direct relationship between methaetnoglobin concentration and the Heinz bodies count; c) the intensity of Heinz bodies in the sickled erythrocytes seems to be independent of the Hb Fetal concentration; d) The genotype SS is more susceptible to Hb oxidation with the release of products of oxidative degradation; e) methaemoglobin formation in blood of people with Hb AA and Hb AS, assessed over 24, 48, 72 and 163 hourly-periods, showed greater oxidative intensity in the Hb AS compared with Hb AA. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-12-01 2014-05-27T11:20:21Z 2014-05-27T11:20:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1516-84842001000100009 Revista Brasileira de Hematologia e Hemoterapia, v. 23, n. 1, p. 53-54, 2001. 1516-8484 http://hdl.handle.net/11449/66694 10.1590/S1516-84842001000100009 S1516-84842001000100009 2-s2.0-52549115553 2-s2.0-52549115553.pdf |
url |
http://dx.doi.org/10.1590/S1516-84842001000100009 http://hdl.handle.net/11449/66694 |
identifier_str_mv |
Revista Brasileira de Hematologia e Hemoterapia, v. 23, n. 1, p. 53-54, 2001. 1516-8484 10.1590/S1516-84842001000100009 S1516-84842001000100009 2-s2.0-52549115553 2-s2.0-52549115553.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Revista Brasileira de Hematologia e Hemoterapia 0,335 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
53-54 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128680797405184 |