Photobiomodulation using LLLT and LED of cells involved in osseointegration and peri-implant soft tissue healing
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10103-021-03299-w http://hdl.handle.net/11449/206223 |
Resumo: | This study evaluated the influence of photobiomodulation (PBM) using low-level laser therapy (PBM/LLLT) or light-emitting diode (PBM/LED) therapy on peri-implant tissue healing. A laboratory model was used to assess the adhesion and metabolism of osteoblasts (SaOs-2), human gingival fibroblasts (HGF), and normal oral keratinocytes (NOK) seeded on a titanium (Ti) surface. After seeding the cells on disks of Ti placed in wells of 24-well plates, three irradiations were performed every 24 h at energy density of 3 J/cm2. For PBM/LLLT, a LaserTABLE device was used with a wavelength of 780 nm and 25 mW, while for PBM/LED irradiation, a LEDTABLE device was used at 810 nm, 20 mW, at a density of 3 J/cm2. After irradiations, the number of cells (NC) attached and spread on the Ti surface, cell viability (CV), total protein (TP), and collagen (Col) synthesis were assessed. Alkaline phosphate activity (ALP) was evaluated only for SaOs-2. Data were submitted to ANOVA complemented by Turkey statistical tests at a 5% significance level. PBM significantly increased adherence of NOK to the Ti surface, while no significant effect was observed for SaOs-2 and HGF. PBM positively affected CV, as well as Col and TP synthesis, in distinct patterns according to the cell line. Increased ALP activity was observed only in those cells exposed to PBM/LLLT. Considering cell specificity, this investigation reports that photobiomodulation with low-power laser and LED at determined parameters enhances cellular functions related to peri-implant tissue healing in a laboratory model. |
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Repositório Institucional da UNESP |
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spelling |
Photobiomodulation using LLLT and LED of cells involved in osseointegration and peri-implant soft tissue healingCell cultureImplantologyLight-emitting diodesLow-level laser therapyTitaniumThis study evaluated the influence of photobiomodulation (PBM) using low-level laser therapy (PBM/LLLT) or light-emitting diode (PBM/LED) therapy on peri-implant tissue healing. A laboratory model was used to assess the adhesion and metabolism of osteoblasts (SaOs-2), human gingival fibroblasts (HGF), and normal oral keratinocytes (NOK) seeded on a titanium (Ti) surface. After seeding the cells on disks of Ti placed in wells of 24-well plates, three irradiations were performed every 24 h at energy density of 3 J/cm2. For PBM/LLLT, a LaserTABLE device was used with a wavelength of 780 nm and 25 mW, while for PBM/LED irradiation, a LEDTABLE device was used at 810 nm, 20 mW, at a density of 3 J/cm2. After irradiations, the number of cells (NC) attached and spread on the Ti surface, cell viability (CV), total protein (TP), and collagen (Col) synthesis were assessed. Alkaline phosphate activity (ALP) was evaluated only for SaOs-2. Data were submitted to ANOVA complemented by Turkey statistical tests at a 5% significance level. PBM significantly increased adherence of NOK to the Ti surface, while no significant effect was observed for SaOs-2 and HGF. PBM positively affected CV, as well as Col and TP synthesis, in distinct patterns according to the cell line. Increased ALP activity was observed only in those cells exposed to PBM/LLLT. Considering cell specificity, this investigation reports that photobiomodulation with low-power laser and LED at determined parameters enhances cellular functions related to peri-implant tissue healing in a laboratory model.Universidade de Ribeirão Preto UNAERPAraraquara Dental School UNESPDepartment of Dentistry Ribeirão Preto University (UNAERP), 2201 Costábile Romano AvenueAraraquara Dental School UNESPUNAERPUniversidade Estadual Paulista (Unesp)Ribeirão Preto University (UNAERP)Rech, Carlos AlbertoPansani, Taisa Nogueira [UNESP]Cardoso, Lais Medeiros [UNESP]Ribeiro, Isabela MassaroSilva-Sousa, Yara Teresinha Correade Souza Costa, Carlos Alberto [UNESP]Basso, Fernanda Gonçalves2021-06-25T10:28:31Z2021-06-25T10:28:31Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s10103-021-03299-wLasers in Medical Science.1435-604X0268-8921http://hdl.handle.net/11449/20622310.1007/s10103-021-03299-w2-s2.0-85104462607Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLasers in Medical Scienceinfo:eu-repo/semantics/openAccess2021-10-23T00:00:06Zoai:repositorio.unesp.br:11449/206223Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T00:00:06Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Photobiomodulation using LLLT and LED of cells involved in osseointegration and peri-implant soft tissue healing |
title |
Photobiomodulation using LLLT and LED of cells involved in osseointegration and peri-implant soft tissue healing |
spellingShingle |
Photobiomodulation using LLLT and LED of cells involved in osseointegration and peri-implant soft tissue healing Rech, Carlos Alberto Cell culture Implantology Light-emitting diodes Low-level laser therapy Titanium |
title_short |
Photobiomodulation using LLLT and LED of cells involved in osseointegration and peri-implant soft tissue healing |
title_full |
Photobiomodulation using LLLT and LED of cells involved in osseointegration and peri-implant soft tissue healing |
title_fullStr |
Photobiomodulation using LLLT and LED of cells involved in osseointegration and peri-implant soft tissue healing |
title_full_unstemmed |
Photobiomodulation using LLLT and LED of cells involved in osseointegration and peri-implant soft tissue healing |
title_sort |
Photobiomodulation using LLLT and LED of cells involved in osseointegration and peri-implant soft tissue healing |
author |
Rech, Carlos Alberto |
author_facet |
Rech, Carlos Alberto Pansani, Taisa Nogueira [UNESP] Cardoso, Lais Medeiros [UNESP] Ribeiro, Isabela Massaro Silva-Sousa, Yara Teresinha Correa de Souza Costa, Carlos Alberto [UNESP] Basso, Fernanda Gonçalves |
author_role |
author |
author2 |
Pansani, Taisa Nogueira [UNESP] Cardoso, Lais Medeiros [UNESP] Ribeiro, Isabela Massaro Silva-Sousa, Yara Teresinha Correa de Souza Costa, Carlos Alberto [UNESP] Basso, Fernanda Gonçalves |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
UNAERP Universidade Estadual Paulista (Unesp) Ribeirão Preto University (UNAERP) |
dc.contributor.author.fl_str_mv |
Rech, Carlos Alberto Pansani, Taisa Nogueira [UNESP] Cardoso, Lais Medeiros [UNESP] Ribeiro, Isabela Massaro Silva-Sousa, Yara Teresinha Correa de Souza Costa, Carlos Alberto [UNESP] Basso, Fernanda Gonçalves |
dc.subject.por.fl_str_mv |
Cell culture Implantology Light-emitting diodes Low-level laser therapy Titanium |
topic |
Cell culture Implantology Light-emitting diodes Low-level laser therapy Titanium |
description |
This study evaluated the influence of photobiomodulation (PBM) using low-level laser therapy (PBM/LLLT) or light-emitting diode (PBM/LED) therapy on peri-implant tissue healing. A laboratory model was used to assess the adhesion and metabolism of osteoblasts (SaOs-2), human gingival fibroblasts (HGF), and normal oral keratinocytes (NOK) seeded on a titanium (Ti) surface. After seeding the cells on disks of Ti placed in wells of 24-well plates, three irradiations were performed every 24 h at energy density of 3 J/cm2. For PBM/LLLT, a LaserTABLE device was used with a wavelength of 780 nm and 25 mW, while for PBM/LED irradiation, a LEDTABLE device was used at 810 nm, 20 mW, at a density of 3 J/cm2. After irradiations, the number of cells (NC) attached and spread on the Ti surface, cell viability (CV), total protein (TP), and collagen (Col) synthesis were assessed. Alkaline phosphate activity (ALP) was evaluated only for SaOs-2. Data were submitted to ANOVA complemented by Turkey statistical tests at a 5% significance level. PBM significantly increased adherence of NOK to the Ti surface, while no significant effect was observed for SaOs-2 and HGF. PBM positively affected CV, as well as Col and TP synthesis, in distinct patterns according to the cell line. Increased ALP activity was observed only in those cells exposed to PBM/LLLT. Considering cell specificity, this investigation reports that photobiomodulation with low-power laser and LED at determined parameters enhances cellular functions related to peri-implant tissue healing in a laboratory model. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:28:31Z 2021-06-25T10:28:31Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10103-021-03299-w Lasers in Medical Science. 1435-604X 0268-8921 http://hdl.handle.net/11449/206223 10.1007/s10103-021-03299-w 2-s2.0-85104462607 |
url |
http://dx.doi.org/10.1007/s10103-021-03299-w http://hdl.handle.net/11449/206223 |
identifier_str_mv |
Lasers in Medical Science. 1435-604X 0268-8921 10.1007/s10103-021-03299-w 2-s2.0-85104462607 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lasers in Medical Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965534960222208 |