Cytogenetic analysis in Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil

Detalhes bibliográficos
Autor(a) principal: Silva, Edson Lourenco da [UNESP]
Data de Publicação: 2012
Outros Autores: Borba, Rafael Splendore de [UNESP], Centofante, Liano, Miyazawa, Carlos Suetoshi, Parise-Maltempi, Patricia Pasquali [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3897/CompCytogen.v6i3.3637
http://hdl.handle.net/11449/196024
Resumo: Thoracocharaz stellatus (Characiformes, Gasteropelecidae) is a small Neotropical species of fish, widely distributed in several rivers of South America. Evidence for karyotype heteromorphysm in populations from different geographical regions has been reported for this species. In this way, populations of T stellatus from the Paraguay River basin were cytogenetically characterized and the results were compared with other studies performed in the same species but from different basins. The results showed a diploid number of 2n = 54 for T stellatus, with chromosomes arranged in 6 metacentric (m), 6 submetacentric (sm), 2 subtelocentric (st) and 40 acrocentric (a), for both sexes, with a simple Nucleolus Organiser Region (NOR) system reported by the techniques of silver nitrate impregnation and fluorescent in situ hybridisation (FISH) using 18S rDNA sequences as probe. The distribution of constitutive heterochromatin, observed by the C-band technique and Chromomycin A3 staining showed great similarity among the analyzed populations and consists mainly of discrete blocks in the pericentromeric and telomeric regions of most chromosomes. The presence of female heterogamety was also observed indicating a ZZ/ZW system with W chromosome almost totally heterochromatic. The results also show cytogenetic diversity of the group and are useful to understand the mechanisms of karyotype evolution of the family.
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spelling Cytogenetic analysis in Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, BrazilThoracocharaz stellatusAg-NORC-bandFISH18S rDNAThoracocharaz stellatus (Characiformes, Gasteropelecidae) is a small Neotropical species of fish, widely distributed in several rivers of South America. Evidence for karyotype heteromorphysm in populations from different geographical regions has been reported for this species. In this way, populations of T stellatus from the Paraguay River basin were cytogenetically characterized and the results were compared with other studies performed in the same species but from different basins. The results showed a diploid number of 2n = 54 for T stellatus, with chromosomes arranged in 6 metacentric (m), 6 submetacentric (sm), 2 subtelocentric (st) and 40 acrocentric (a), for both sexes, with a simple Nucleolus Organiser Region (NOR) system reported by the techniques of silver nitrate impregnation and fluorescent in situ hybridisation (FISH) using 18S rDNA sequences as probe. The distribution of constitutive heterochromatin, observed by the C-band technique and Chromomycin A3 staining showed great similarity among the analyzed populations and consists mainly of discrete blocks in the pericentromeric and telomeric regions of most chromosomes. The presence of female heterogamety was also observed indicating a ZZ/ZW system with W chromosome almost totally heterochromatic. The results also show cytogenetic diversity of the group and are useful to understand the mechanisms of karyotype evolution of the family.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)FAPEMATBASAUFMTUNESP Univ Estadual Paulista, Inst Biociencias, Dept Biol, Lab Citogenet, BR-13506900 Rio Claro, SP, BrazilUFMT Univ Fed Mato Grosso, Inst Biociencias, Dept Biol & Zool, Lab Citogenet Anim, BR-78060900 Cuiaba, MT, BrazilUniv Fed ABC, CCNH, BR-09210170 Santo Andre, SP, BrazilUNESP Univ Estadual Paulista, Inst Biociencias, Dept Biol, Lab Citogenet, BR-13506900 Rio Claro, SP, BrazilPensoft PublishersUniversidade Estadual Paulista (Unesp)Universidade Federal de Mato Grosso do Sul (UFMS)Universidade Federal do ABC (UFABC)Silva, Edson Lourenco da [UNESP]Borba, Rafael Splendore de [UNESP]Centofante, LianoMiyazawa, Carlos SuetoshiParise-Maltempi, Patricia Pasquali [UNESP]2020-12-10T19:30:48Z2020-12-10T19:30:48Z2012-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article323-333http://dx.doi.org/10.3897/CompCytogen.v6i3.3637Comparative Cytogenetics. Sofia: Pensoft Publishers, v. 6, n. 3, p. 323-333, 2012.1993-0771http://hdl.handle.net/11449/19602410.3897/CompCytogen.v6i3.3637WOS:000311066300009Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengComparative Cytogeneticsinfo:eu-repo/semantics/openAccess2021-10-23T02:54:13Zoai:repositorio.unesp.br:11449/196024Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:23:33.773523Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cytogenetic analysis in Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil
title Cytogenetic analysis in Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil
spellingShingle Cytogenetic analysis in Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil
Silva, Edson Lourenco da [UNESP]
Thoracocharaz stellatus
Ag-NOR
C-band
FISH
18S rDNA
title_short Cytogenetic analysis in Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil
title_full Cytogenetic analysis in Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil
title_fullStr Cytogenetic analysis in Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil
title_full_unstemmed Cytogenetic analysis in Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil
title_sort Cytogenetic analysis in Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil
author Silva, Edson Lourenco da [UNESP]
author_facet Silva, Edson Lourenco da [UNESP]
Borba, Rafael Splendore de [UNESP]
Centofante, Liano
Miyazawa, Carlos Suetoshi
Parise-Maltempi, Patricia Pasquali [UNESP]
author_role author
author2 Borba, Rafael Splendore de [UNESP]
Centofante, Liano
Miyazawa, Carlos Suetoshi
Parise-Maltempi, Patricia Pasquali [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal de Mato Grosso do Sul (UFMS)
Universidade Federal do ABC (UFABC)
dc.contributor.author.fl_str_mv Silva, Edson Lourenco da [UNESP]
Borba, Rafael Splendore de [UNESP]
Centofante, Liano
Miyazawa, Carlos Suetoshi
Parise-Maltempi, Patricia Pasquali [UNESP]
dc.subject.por.fl_str_mv Thoracocharaz stellatus
Ag-NOR
C-band
FISH
18S rDNA
topic Thoracocharaz stellatus
Ag-NOR
C-band
FISH
18S rDNA
description Thoracocharaz stellatus (Characiformes, Gasteropelecidae) is a small Neotropical species of fish, widely distributed in several rivers of South America. Evidence for karyotype heteromorphysm in populations from different geographical regions has been reported for this species. In this way, populations of T stellatus from the Paraguay River basin were cytogenetically characterized and the results were compared with other studies performed in the same species but from different basins. The results showed a diploid number of 2n = 54 for T stellatus, with chromosomes arranged in 6 metacentric (m), 6 submetacentric (sm), 2 subtelocentric (st) and 40 acrocentric (a), for both sexes, with a simple Nucleolus Organiser Region (NOR) system reported by the techniques of silver nitrate impregnation and fluorescent in situ hybridisation (FISH) using 18S rDNA sequences as probe. The distribution of constitutive heterochromatin, observed by the C-band technique and Chromomycin A3 staining showed great similarity among the analyzed populations and consists mainly of discrete blocks in the pericentromeric and telomeric regions of most chromosomes. The presence of female heterogamety was also observed indicating a ZZ/ZW system with W chromosome almost totally heterochromatic. The results also show cytogenetic diversity of the group and are useful to understand the mechanisms of karyotype evolution of the family.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
2020-12-10T19:30:48Z
2020-12-10T19:30:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3897/CompCytogen.v6i3.3637
Comparative Cytogenetics. Sofia: Pensoft Publishers, v. 6, n. 3, p. 323-333, 2012.
1993-0771
http://hdl.handle.net/11449/196024
10.3897/CompCytogen.v6i3.3637
WOS:000311066300009
url http://dx.doi.org/10.3897/CompCytogen.v6i3.3637
http://hdl.handle.net/11449/196024
identifier_str_mv Comparative Cytogenetics. Sofia: Pensoft Publishers, v. 6, n. 3, p. 323-333, 2012.
1993-0771
10.3897/CompCytogen.v6i3.3637
WOS:000311066300009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Comparative Cytogenetics
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 323-333
dc.publisher.none.fl_str_mv Pensoft Publishers
publisher.none.fl_str_mv Pensoft Publishers
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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