Endocrine disruptive action of diclofenac and caffeine on Astyanax altiparanae males (Teleostei: Characiformes: Characidae)

Detalhes bibliográficos
Autor(a) principal: Godoi, Filipe G.A.
Data de Publicação: 2020
Outros Autores: Muñoz-Peñuela, Marcela, Gomes, Aline D. Olio, Tolussi, Carlos E., Brambila-Souza, Gabriela [UNESP], Branco, Giovana S. [UNESP], Lo Nostro, Fabiana L., Moreira, Renata G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.cbpc.2020.108720
http://hdl.handle.net/11449/200023
Resumo: Diclofenac (DCF) and caffeine (CAF) are persistent pharmaceuticals that occur in mixtures in the aquatic ecosystems causing effects in the reproductive physiology of aquatic organisms. This study evaluated the physiological reproductive responses of Astyanax altiparanae males exposed to nominal concentrations of DCF (3.08 mg L− 1) and CAF (9.59 mg L− 1) separately and combined, for 96 h. The steroids profile, estrogenic biomarker vitellogenin (vtgA), testes and liver morphology, and also mortality of males were assessed. DCF and CAF degradation was 5% of the initial concentration for 24 h. The LC50 of the DCF and CAF were 30.8 mg L− 1 and 95.9 mg L− 1, respectively. Males exposed to DCF and CAF exhibited a reduction of 17β-Estradiol (E2) concentration compared to control (CTL). Similarly, testosterone (T) was also reduced in the DCF treatment, but this response was not observed in 11-Ketotestosterone (11-KT). Males exposed to DCF + CAF combined did not exhibit differences in T, E2 and 11-KT steroids. The vtgA gene expression and the sperm concentration did not change among the treatments. Moreover, acute exposure revealed a hypertrophy of hepatocytes cells in the DCF and DCF + CAF treatments. In conclusion, DCF and CAF, isolated, exhibit an endocrine disruptive activity in A. altiparanae male, an opposite response observed with the mixture of both compounds that abolishes the endocrine disruptive effects. DCF seems to be more toxic for this species, altering also hepatocytes morphology.
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spelling Endocrine disruptive action of diclofenac and caffeine on Astyanax altiparanae males (Teleostei: Characiformes: Characidae)Endocrine disrupting chemicalsFishPharmaceuticalsSteroidogenesisVitellogenin aDiclofenac (DCF) and caffeine (CAF) are persistent pharmaceuticals that occur in mixtures in the aquatic ecosystems causing effects in the reproductive physiology of aquatic organisms. This study evaluated the physiological reproductive responses of Astyanax altiparanae males exposed to nominal concentrations of DCF (3.08 mg L− 1) and CAF (9.59 mg L− 1) separately and combined, for 96 h. The steroids profile, estrogenic biomarker vitellogenin (vtgA), testes and liver morphology, and also mortality of males were assessed. DCF and CAF degradation was 5% of the initial concentration for 24 h. The LC50 of the DCF and CAF were 30.8 mg L− 1 and 95.9 mg L− 1, respectively. Males exposed to DCF and CAF exhibited a reduction of 17β-Estradiol (E2) concentration compared to control (CTL). Similarly, testosterone (T) was also reduced in the DCF treatment, but this response was not observed in 11-Ketotestosterone (11-KT). Males exposed to DCF + CAF combined did not exhibit differences in T, E2 and 11-KT steroids. The vtgA gene expression and the sperm concentration did not change among the treatments. Moreover, acute exposure revealed a hypertrophy of hepatocytes cells in the DCF and DCF + CAF treatments. In conclusion, DCF and CAF, isolated, exhibit an endocrine disruptive activity in A. altiparanae male, an opposite response observed with the mixture of both compounds that abolishes the endocrine disruptive effects. DCF seems to be more toxic for this species, altering also hepatocytes morphology.Companhia Energética de São PauloCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Fisiologia Instituto de Biociências Universidade de São Paulo, Rua do Matão, Trav.14, n° 321Universidade Anhembi-Morumbi, Campus MoocaUNESP - Universidade Estadual Paulista - Centro de Aquicultura (CAUNESP)Lab. de Ecotoxicología Acuática Departamento de Biodiversidad y Biología Experimental Facultad de Ciencias Exactas y Naturales Universidad de Buenos Aires & IBBEA CONICET-UBAUNESP - Universidade Estadual Paulista - Centro de Aquicultura (CAUNESP)CAPES: 001CNPq: 130190/2017-9FAPESP: 2014/16320-7FAPESP: 2017/07139-5FAPESP: 2017/11530-1Universidade de São Paulo (USP)Universidade Anhembi-MorumbiUniversidade Estadual Paulista (Unesp)CONICET-UBAGodoi, Filipe G.A.Muñoz-Peñuela, MarcelaGomes, Aline D. OlioTolussi, Carlos E.Brambila-Souza, Gabriela [UNESP]Branco, Giovana S. [UNESP]Lo Nostro, Fabiana L.Moreira, Renata G.2020-12-12T01:55:30Z2020-12-12T01:55:30Z2020-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.cbpc.2020.108720Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, v. 231.1878-16591532-0456http://hdl.handle.net/11449/20002310.1016/j.cbpc.2020.1087202-s2.0-85078837000Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengComparative Biochemistry and Physiology Part - C: Toxicology and Pharmacologyinfo:eu-repo/semantics/openAccess2024-04-09T15:36:42Zoai:repositorio.unesp.br:11449/200023Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:17:19.599017Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Endocrine disruptive action of diclofenac and caffeine on Astyanax altiparanae males (Teleostei: Characiformes: Characidae)
title Endocrine disruptive action of diclofenac and caffeine on Astyanax altiparanae males (Teleostei: Characiformes: Characidae)
spellingShingle Endocrine disruptive action of diclofenac and caffeine on Astyanax altiparanae males (Teleostei: Characiformes: Characidae)
Godoi, Filipe G.A.
Endocrine disrupting chemicals
Fish
Pharmaceuticals
Steroidogenesis
Vitellogenin a
title_short Endocrine disruptive action of diclofenac and caffeine on Astyanax altiparanae males (Teleostei: Characiformes: Characidae)
title_full Endocrine disruptive action of diclofenac and caffeine on Astyanax altiparanae males (Teleostei: Characiformes: Characidae)
title_fullStr Endocrine disruptive action of diclofenac and caffeine on Astyanax altiparanae males (Teleostei: Characiformes: Characidae)
title_full_unstemmed Endocrine disruptive action of diclofenac and caffeine on Astyanax altiparanae males (Teleostei: Characiformes: Characidae)
title_sort Endocrine disruptive action of diclofenac and caffeine on Astyanax altiparanae males (Teleostei: Characiformes: Characidae)
author Godoi, Filipe G.A.
author_facet Godoi, Filipe G.A.
Muñoz-Peñuela, Marcela
Gomes, Aline D. Olio
Tolussi, Carlos E.
Brambila-Souza, Gabriela [UNESP]
Branco, Giovana S. [UNESP]
Lo Nostro, Fabiana L.
Moreira, Renata G.
author_role author
author2 Muñoz-Peñuela, Marcela
Gomes, Aline D. Olio
Tolussi, Carlos E.
Brambila-Souza, Gabriela [UNESP]
Branco, Giovana S. [UNESP]
Lo Nostro, Fabiana L.
Moreira, Renata G.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Anhembi-Morumbi
Universidade Estadual Paulista (Unesp)
CONICET-UBA
dc.contributor.author.fl_str_mv Godoi, Filipe G.A.
Muñoz-Peñuela, Marcela
Gomes, Aline D. Olio
Tolussi, Carlos E.
Brambila-Souza, Gabriela [UNESP]
Branco, Giovana S. [UNESP]
Lo Nostro, Fabiana L.
Moreira, Renata G.
dc.subject.por.fl_str_mv Endocrine disrupting chemicals
Fish
Pharmaceuticals
Steroidogenesis
Vitellogenin a
topic Endocrine disrupting chemicals
Fish
Pharmaceuticals
Steroidogenesis
Vitellogenin a
description Diclofenac (DCF) and caffeine (CAF) are persistent pharmaceuticals that occur in mixtures in the aquatic ecosystems causing effects in the reproductive physiology of aquatic organisms. This study evaluated the physiological reproductive responses of Astyanax altiparanae males exposed to nominal concentrations of DCF (3.08 mg L− 1) and CAF (9.59 mg L− 1) separately and combined, for 96 h. The steroids profile, estrogenic biomarker vitellogenin (vtgA), testes and liver morphology, and also mortality of males were assessed. DCF and CAF degradation was 5% of the initial concentration for 24 h. The LC50 of the DCF and CAF were 30.8 mg L− 1 and 95.9 mg L− 1, respectively. Males exposed to DCF and CAF exhibited a reduction of 17β-Estradiol (E2) concentration compared to control (CTL). Similarly, testosterone (T) was also reduced in the DCF treatment, but this response was not observed in 11-Ketotestosterone (11-KT). Males exposed to DCF + CAF combined did not exhibit differences in T, E2 and 11-KT steroids. The vtgA gene expression and the sperm concentration did not change among the treatments. Moreover, acute exposure revealed a hypertrophy of hepatocytes cells in the DCF and DCF + CAF treatments. In conclusion, DCF and CAF, isolated, exhibit an endocrine disruptive activity in A. altiparanae male, an opposite response observed with the mixture of both compounds that abolishes the endocrine disruptive effects. DCF seems to be more toxic for this species, altering also hepatocytes morphology.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T01:55:30Z
2020-12-12T01:55:30Z
2020-05-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.cbpc.2020.108720
Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, v. 231.
1878-1659
1532-0456
http://hdl.handle.net/11449/200023
10.1016/j.cbpc.2020.108720
2-s2.0-85078837000
url http://dx.doi.org/10.1016/j.cbpc.2020.108720
http://hdl.handle.net/11449/200023
identifier_str_mv Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, v. 231.
1878-1659
1532-0456
10.1016/j.cbpc.2020.108720
2-s2.0-85078837000
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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