Endocrine disruptive action of diclofenac and caffeine on Astyanax altiparanae males (Teleostei: Characiformes: Characidae)
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.cbpc.2020.108720 http://hdl.handle.net/11449/200023 |
Resumo: | Diclofenac (DCF) and caffeine (CAF) are persistent pharmaceuticals that occur in mixtures in the aquatic ecosystems causing effects in the reproductive physiology of aquatic organisms. This study evaluated the physiological reproductive responses of Astyanax altiparanae males exposed to nominal concentrations of DCF (3.08 mg L− 1) and CAF (9.59 mg L− 1) separately and combined, for 96 h. The steroids profile, estrogenic biomarker vitellogenin (vtgA), testes and liver morphology, and also mortality of males were assessed. DCF and CAF degradation was 5% of the initial concentration for 24 h. The LC50 of the DCF and CAF were 30.8 mg L− 1 and 95.9 mg L− 1, respectively. Males exposed to DCF and CAF exhibited a reduction of 17β-Estradiol (E2) concentration compared to control (CTL). Similarly, testosterone (T) was also reduced in the DCF treatment, but this response was not observed in 11-Ketotestosterone (11-KT). Males exposed to DCF + CAF combined did not exhibit differences in T, E2 and 11-KT steroids. The vtgA gene expression and the sperm concentration did not change among the treatments. Moreover, acute exposure revealed a hypertrophy of hepatocytes cells in the DCF and DCF + CAF treatments. In conclusion, DCF and CAF, isolated, exhibit an endocrine disruptive activity in A. altiparanae male, an opposite response observed with the mixture of both compounds that abolishes the endocrine disruptive effects. DCF seems to be more toxic for this species, altering also hepatocytes morphology. |
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Endocrine disruptive action of diclofenac and caffeine on Astyanax altiparanae males (Teleostei: Characiformes: Characidae)Endocrine disrupting chemicalsFishPharmaceuticalsSteroidogenesisVitellogenin aDiclofenac (DCF) and caffeine (CAF) are persistent pharmaceuticals that occur in mixtures in the aquatic ecosystems causing effects in the reproductive physiology of aquatic organisms. This study evaluated the physiological reproductive responses of Astyanax altiparanae males exposed to nominal concentrations of DCF (3.08 mg L− 1) and CAF (9.59 mg L− 1) separately and combined, for 96 h. The steroids profile, estrogenic biomarker vitellogenin (vtgA), testes and liver morphology, and also mortality of males were assessed. DCF and CAF degradation was 5% of the initial concentration for 24 h. The LC50 of the DCF and CAF were 30.8 mg L− 1 and 95.9 mg L− 1, respectively. Males exposed to DCF and CAF exhibited a reduction of 17β-Estradiol (E2) concentration compared to control (CTL). Similarly, testosterone (T) was also reduced in the DCF treatment, but this response was not observed in 11-Ketotestosterone (11-KT). Males exposed to DCF + CAF combined did not exhibit differences in T, E2 and 11-KT steroids. The vtgA gene expression and the sperm concentration did not change among the treatments. Moreover, acute exposure revealed a hypertrophy of hepatocytes cells in the DCF and DCF + CAF treatments. In conclusion, DCF and CAF, isolated, exhibit an endocrine disruptive activity in A. altiparanae male, an opposite response observed with the mixture of both compounds that abolishes the endocrine disruptive effects. DCF seems to be more toxic for this species, altering also hepatocytes morphology.Companhia Energética de São PauloCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Fisiologia Instituto de Biociências Universidade de São Paulo, Rua do Matão, Trav.14, n° 321Universidade Anhembi-Morumbi, Campus MoocaUNESP - Universidade Estadual Paulista - Centro de Aquicultura (CAUNESP)Lab. de Ecotoxicología Acuática Departamento de Biodiversidad y Biología Experimental Facultad de Ciencias Exactas y Naturales Universidad de Buenos Aires & IBBEA CONICET-UBAUNESP - Universidade Estadual Paulista - Centro de Aquicultura (CAUNESP)CAPES: 001CNPq: 130190/2017-9FAPESP: 2014/16320-7FAPESP: 2017/07139-5FAPESP: 2017/11530-1Universidade de São Paulo (USP)Universidade Anhembi-MorumbiUniversidade Estadual Paulista (Unesp)CONICET-UBAGodoi, Filipe G.A.Muñoz-Peñuela, MarcelaGomes, Aline D. OlioTolussi, Carlos E.Brambila-Souza, Gabriela [UNESP]Branco, Giovana S. [UNESP]Lo Nostro, Fabiana L.Moreira, Renata G.2020-12-12T01:55:30Z2020-12-12T01:55:30Z2020-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.cbpc.2020.108720Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, v. 231.1878-16591532-0456http://hdl.handle.net/11449/20002310.1016/j.cbpc.2020.1087202-s2.0-85078837000Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengComparative Biochemistry and Physiology Part - C: Toxicology and Pharmacologyinfo:eu-repo/semantics/openAccess2024-04-09T15:36:42Zoai:repositorio.unesp.br:11449/200023Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:17:19.599017Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Endocrine disruptive action of diclofenac and caffeine on Astyanax altiparanae males (Teleostei: Characiformes: Characidae) |
title |
Endocrine disruptive action of diclofenac and caffeine on Astyanax altiparanae males (Teleostei: Characiformes: Characidae) |
spellingShingle |
Endocrine disruptive action of diclofenac and caffeine on Astyanax altiparanae males (Teleostei: Characiformes: Characidae) Godoi, Filipe G.A. Endocrine disrupting chemicals Fish Pharmaceuticals Steroidogenesis Vitellogenin a |
title_short |
Endocrine disruptive action of diclofenac and caffeine on Astyanax altiparanae males (Teleostei: Characiformes: Characidae) |
title_full |
Endocrine disruptive action of diclofenac and caffeine on Astyanax altiparanae males (Teleostei: Characiformes: Characidae) |
title_fullStr |
Endocrine disruptive action of diclofenac and caffeine on Astyanax altiparanae males (Teleostei: Characiformes: Characidae) |
title_full_unstemmed |
Endocrine disruptive action of diclofenac and caffeine on Astyanax altiparanae males (Teleostei: Characiformes: Characidae) |
title_sort |
Endocrine disruptive action of diclofenac and caffeine on Astyanax altiparanae males (Teleostei: Characiformes: Characidae) |
author |
Godoi, Filipe G.A. |
author_facet |
Godoi, Filipe G.A. Muñoz-Peñuela, Marcela Gomes, Aline D. Olio Tolussi, Carlos E. Brambila-Souza, Gabriela [UNESP] Branco, Giovana S. [UNESP] Lo Nostro, Fabiana L. Moreira, Renata G. |
author_role |
author |
author2 |
Muñoz-Peñuela, Marcela Gomes, Aline D. Olio Tolussi, Carlos E. Brambila-Souza, Gabriela [UNESP] Branco, Giovana S. [UNESP] Lo Nostro, Fabiana L. Moreira, Renata G. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Anhembi-Morumbi Universidade Estadual Paulista (Unesp) CONICET-UBA |
dc.contributor.author.fl_str_mv |
Godoi, Filipe G.A. Muñoz-Peñuela, Marcela Gomes, Aline D. Olio Tolussi, Carlos E. Brambila-Souza, Gabriela [UNESP] Branco, Giovana S. [UNESP] Lo Nostro, Fabiana L. Moreira, Renata G. |
dc.subject.por.fl_str_mv |
Endocrine disrupting chemicals Fish Pharmaceuticals Steroidogenesis Vitellogenin a |
topic |
Endocrine disrupting chemicals Fish Pharmaceuticals Steroidogenesis Vitellogenin a |
description |
Diclofenac (DCF) and caffeine (CAF) are persistent pharmaceuticals that occur in mixtures in the aquatic ecosystems causing effects in the reproductive physiology of aquatic organisms. This study evaluated the physiological reproductive responses of Astyanax altiparanae males exposed to nominal concentrations of DCF (3.08 mg L− 1) and CAF (9.59 mg L− 1) separately and combined, for 96 h. The steroids profile, estrogenic biomarker vitellogenin (vtgA), testes and liver morphology, and also mortality of males were assessed. DCF and CAF degradation was 5% of the initial concentration for 24 h. The LC50 of the DCF and CAF were 30.8 mg L− 1 and 95.9 mg L− 1, respectively. Males exposed to DCF and CAF exhibited a reduction of 17β-Estradiol (E2) concentration compared to control (CTL). Similarly, testosterone (T) was also reduced in the DCF treatment, but this response was not observed in 11-Ketotestosterone (11-KT). Males exposed to DCF + CAF combined did not exhibit differences in T, E2 and 11-KT steroids. The vtgA gene expression and the sperm concentration did not change among the treatments. Moreover, acute exposure revealed a hypertrophy of hepatocytes cells in the DCF and DCF + CAF treatments. In conclusion, DCF and CAF, isolated, exhibit an endocrine disruptive activity in A. altiparanae male, an opposite response observed with the mixture of both compounds that abolishes the endocrine disruptive effects. DCF seems to be more toxic for this species, altering also hepatocytes morphology. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:55:30Z 2020-12-12T01:55:30Z 2020-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.cbpc.2020.108720 Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, v. 231. 1878-1659 1532-0456 http://hdl.handle.net/11449/200023 10.1016/j.cbpc.2020.108720 2-s2.0-85078837000 |
url |
http://dx.doi.org/10.1016/j.cbpc.2020.108720 http://hdl.handle.net/11449/200023 |
identifier_str_mv |
Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, v. 231. 1878-1659 1532-0456 10.1016/j.cbpc.2020.108720 2-s2.0-85078837000 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129047186636800 |