Gastroprotective effect of limonene in rats: Influence on oxidative stress, inflammation and gene expression
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.phymed.2018.09.027 http://hdl.handle.net/11449/189841 |
Resumo: | Background: In an increasing search for natural products that may heal the ulcers and avoid its recurrence, limonene appears as a promising candidate. Hypothesis/Purpose: The present study aimed to investigate the protective effect of limonene in ethanol-induced gastric ulcers, in addition, to investigate the involvement of antioxidant and anti-inflammatory activities, besides the modulation of gene expression. Study Design: Male Wistar rats were orally treated with vehicle (8% tween 80), carbenoxolone (100 mg/kg) or limonene (25, 50 or 100 mg/kg) and then orally received ethanol to induce gastric ulcers formation. Methods: The activity of myeloperoxidase (MPO) was measured. Levels of glutathione (GSH) and activities of glutathione peroxidase (GPx), glutathione reductase (GR) and superoxide dismutase (SOD) were measured. We investigated the anti-inflammatory effect of limonene measuring the levels of pro-inflammatory cytokines tumor necrosis factor-a (TNF-a), interleukin-6 (IL-6), interleukin-1β (IL-1β) and anti-inflammatory cytokine interleukin-10 (IL-10) by ELISA. Additionally, we investigate through real-time PCR (qPCR) the gene expression of nuclear factor-kappa B (Nf-κb), Gpx, Il-1β Mpo, and Il-10. Results: Our results showed that limonene 50 mg/kg was the lowest effective dose, offering 93% of reduction in gastric ulcer area compared with the vehicle. There was an increase in mucus production and higher preservation of gastric mucosa integrity after treatment with limonene.There was a reduction in the MPO activity, a biomarker of neutrophils infiltration, and an increase in GPx activity, suggesting an antioxidant effect. Limonene displayed anti-inflammatory activity through decreasing the levels of TNF-a, IL-6, and IL-1β and increasing the level of IL-10. Limonene could down-regulate the expression of Nf-κb, Il-1β and Mpo and up-regulate the expression of Gpx. Conclusion: Our results demonstrate that oral treatment with limonene exerts gastroprotection through local mucosal defense mechanisms, such as increasing the mucus production, modulation of the oxidative stress and inflammatory response and inhibition of Nf-κb expression. |
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Gastroprotective effect of limonene in rats: Influence on oxidative stress, inflammation and gene expressionAntioxidantGastric ulcerGene expressionInflammationLimoneneqPCRBackground: In an increasing search for natural products that may heal the ulcers and avoid its recurrence, limonene appears as a promising candidate. Hypothesis/Purpose: The present study aimed to investigate the protective effect of limonene in ethanol-induced gastric ulcers, in addition, to investigate the involvement of antioxidant and anti-inflammatory activities, besides the modulation of gene expression. Study Design: Male Wistar rats were orally treated with vehicle (8% tween 80), carbenoxolone (100 mg/kg) or limonene (25, 50 or 100 mg/kg) and then orally received ethanol to induce gastric ulcers formation. Methods: The activity of myeloperoxidase (MPO) was measured. Levels of glutathione (GSH) and activities of glutathione peroxidase (GPx), glutathione reductase (GR) and superoxide dismutase (SOD) were measured. We investigated the anti-inflammatory effect of limonene measuring the levels of pro-inflammatory cytokines tumor necrosis factor-a (TNF-a), interleukin-6 (IL-6), interleukin-1β (IL-1β) and anti-inflammatory cytokine interleukin-10 (IL-10) by ELISA. Additionally, we investigate through real-time PCR (qPCR) the gene expression of nuclear factor-kappa B (Nf-κb), Gpx, Il-1β Mpo, and Il-10. Results: Our results showed that limonene 50 mg/kg was the lowest effective dose, offering 93% of reduction in gastric ulcer area compared with the vehicle. There was an increase in mucus production and higher preservation of gastric mucosa integrity after treatment with limonene.There was a reduction in the MPO activity, a biomarker of neutrophils infiltration, and an increase in GPx activity, suggesting an antioxidant effect. Limonene displayed anti-inflammatory activity through decreasing the levels of TNF-a, IL-6, and IL-1β and increasing the level of IL-10. Limonene could down-regulate the expression of Nf-κb, Il-1β and Mpo and up-regulate the expression of Gpx. Conclusion: Our results demonstrate that oral treatment with limonene exerts gastroprotection through local mucosal defense mechanisms, such as increasing the mucus production, modulation of the oxidative stress and inflammatory response and inhibition of Nf-κb expression.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Morphology São Paulo State University (UNESP) Institute of BiosciencesDepartment of Morphology São Paulo State University (UNESP) Institute of BiosciencesFAPESP: 2016/25832-7Universidade Estadual Paulista (Unesp)de Souza, Matheus Chiaradia [UNESP]Vieira, Ana Júlia [UNESP]Beserra, Fernando Pereira [UNESP]Pellizzon, Cláudia Helena [UNESP]Nóbrega, Rafael Henrique [UNESP]Rozza, Ariane Leite [UNESP]2019-10-06T16:53:53Z2019-10-06T16:53:53Z2019-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article37-42http://dx.doi.org/10.1016/j.phymed.2018.09.027Phytomedicine, v. 53, p. 37-42.1618-095X0944-7113http://hdl.handle.net/11449/18984110.1016/j.phymed.2018.09.0272-s2.0-85055639066051570858525398500193937798010690000-0001-9796-50760000-0002-4494-4180Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhytomedicineinfo:eu-repo/semantics/openAccess2021-10-27T11:18:52Zoai:repositorio.unesp.br:11449/189841Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-27T11:18:52Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Gastroprotective effect of limonene in rats: Influence on oxidative stress, inflammation and gene expression |
title |
Gastroprotective effect of limonene in rats: Influence on oxidative stress, inflammation and gene expression |
spellingShingle |
Gastroprotective effect of limonene in rats: Influence on oxidative stress, inflammation and gene expression de Souza, Matheus Chiaradia [UNESP] Antioxidant Gastric ulcer Gene expression Inflammation Limonene qPCR |
title_short |
Gastroprotective effect of limonene in rats: Influence on oxidative stress, inflammation and gene expression |
title_full |
Gastroprotective effect of limonene in rats: Influence on oxidative stress, inflammation and gene expression |
title_fullStr |
Gastroprotective effect of limonene in rats: Influence on oxidative stress, inflammation and gene expression |
title_full_unstemmed |
Gastroprotective effect of limonene in rats: Influence on oxidative stress, inflammation and gene expression |
title_sort |
Gastroprotective effect of limonene in rats: Influence on oxidative stress, inflammation and gene expression |
author |
de Souza, Matheus Chiaradia [UNESP] |
author_facet |
de Souza, Matheus Chiaradia [UNESP] Vieira, Ana Júlia [UNESP] Beserra, Fernando Pereira [UNESP] Pellizzon, Cláudia Helena [UNESP] Nóbrega, Rafael Henrique [UNESP] Rozza, Ariane Leite [UNESP] |
author_role |
author |
author2 |
Vieira, Ana Júlia [UNESP] Beserra, Fernando Pereira [UNESP] Pellizzon, Cláudia Helena [UNESP] Nóbrega, Rafael Henrique [UNESP] Rozza, Ariane Leite [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
de Souza, Matheus Chiaradia [UNESP] Vieira, Ana Júlia [UNESP] Beserra, Fernando Pereira [UNESP] Pellizzon, Cláudia Helena [UNESP] Nóbrega, Rafael Henrique [UNESP] Rozza, Ariane Leite [UNESP] |
dc.subject.por.fl_str_mv |
Antioxidant Gastric ulcer Gene expression Inflammation Limonene qPCR |
topic |
Antioxidant Gastric ulcer Gene expression Inflammation Limonene qPCR |
description |
Background: In an increasing search for natural products that may heal the ulcers and avoid its recurrence, limonene appears as a promising candidate. Hypothesis/Purpose: The present study aimed to investigate the protective effect of limonene in ethanol-induced gastric ulcers, in addition, to investigate the involvement of antioxidant and anti-inflammatory activities, besides the modulation of gene expression. Study Design: Male Wistar rats were orally treated with vehicle (8% tween 80), carbenoxolone (100 mg/kg) or limonene (25, 50 or 100 mg/kg) and then orally received ethanol to induce gastric ulcers formation. Methods: The activity of myeloperoxidase (MPO) was measured. Levels of glutathione (GSH) and activities of glutathione peroxidase (GPx), glutathione reductase (GR) and superoxide dismutase (SOD) were measured. We investigated the anti-inflammatory effect of limonene measuring the levels of pro-inflammatory cytokines tumor necrosis factor-a (TNF-a), interleukin-6 (IL-6), interleukin-1β (IL-1β) and anti-inflammatory cytokine interleukin-10 (IL-10) by ELISA. Additionally, we investigate through real-time PCR (qPCR) the gene expression of nuclear factor-kappa B (Nf-κb), Gpx, Il-1β Mpo, and Il-10. Results: Our results showed that limonene 50 mg/kg was the lowest effective dose, offering 93% of reduction in gastric ulcer area compared with the vehicle. There was an increase in mucus production and higher preservation of gastric mucosa integrity after treatment with limonene.There was a reduction in the MPO activity, a biomarker of neutrophils infiltration, and an increase in GPx activity, suggesting an antioxidant effect. Limonene displayed anti-inflammatory activity through decreasing the levels of TNF-a, IL-6, and IL-1β and increasing the level of IL-10. Limonene could down-regulate the expression of Nf-κb, Il-1β and Mpo and up-regulate the expression of Gpx. Conclusion: Our results demonstrate that oral treatment with limonene exerts gastroprotection through local mucosal defense mechanisms, such as increasing the mucus production, modulation of the oxidative stress and inflammatory response and inhibition of Nf-κb expression. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:53:53Z 2019-10-06T16:53:53Z 2019-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.phymed.2018.09.027 Phytomedicine, v. 53, p. 37-42. 1618-095X 0944-7113 http://hdl.handle.net/11449/189841 10.1016/j.phymed.2018.09.027 2-s2.0-85055639066 0515708585253985 0019393779801069 0000-0001-9796-5076 0000-0002-4494-4180 |
url |
http://dx.doi.org/10.1016/j.phymed.2018.09.027 http://hdl.handle.net/11449/189841 |
identifier_str_mv |
Phytomedicine, v. 53, p. 37-42. 1618-095X 0944-7113 10.1016/j.phymed.2018.09.027 2-s2.0-85055639066 0515708585253985 0019393779801069 0000-0001-9796-5076 0000-0002-4494-4180 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Phytomedicine |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
37-42 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965482859626496 |