First successful frozen semen of the maned wolf (Chrysocyon brachyurus)

Detalhes bibliográficos
Autor(a) principal: Teodoro, Laura O. [UNESP]
Data de Publicação: 2021
Outros Autores: Camargo, Laiza S. [UNESP], Scheeren, Verônica F. C. [UNESP], Freitas-Dell'Aqua, Camila P. [UNESP], Papa, Frederico O. [UNESP], Honsho, Cristiane S., Souza, Fabiana F. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1111/rda.13999
http://hdl.handle.net/11449/229375
Resumo: This study aimed to describe successful cryopreservation of sperm from maned wolves (Chrysocyon brachyurus). Three ejaculates from 2 maned wolves were collected by digital manipulation of the penis and evaluated subjectively, centrifuged and frozen in BotuCrio® (Botupharma, Botucatu, Brazil) or Tris–yolk egg extender. Spermatozoa were thawed at 37ºC/30s or 70ºC/4s and evaluated for kinetics, morphology, plasma and acrosome membrane integrity, mitochondrial potential, hydrogen peroxide, superoxide anion and lipid peroxidation. From 5 thawed samples, two had sperm total motility >55% (56.0% and 64.0%) and progressive motility ~35% (35% and 40%), both frozen with Tris–yolk egg. Plasma and acrosome membrane integrity decreased and percentage of sperm defects increased post-thawing. We concluded that is possible to freeze spermatozoa from maned wolves using semen collection and processing methods applied for domestic dogs.
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spelling First successful frozen semen of the maned wolf (Chrysocyon brachyurus)captive-wolfcryopreservationspermatozoaThis study aimed to describe successful cryopreservation of sperm from maned wolves (Chrysocyon brachyurus). Three ejaculates from 2 maned wolves were collected by digital manipulation of the penis and evaluated subjectively, centrifuged and frozen in BotuCrio® (Botupharma, Botucatu, Brazil) or Tris–yolk egg extender. Spermatozoa were thawed at 37ºC/30s or 70ºC/4s and evaluated for kinetics, morphology, plasma and acrosome membrane integrity, mitochondrial potential, hydrogen peroxide, superoxide anion and lipid peroxidation. From 5 thawed samples, two had sperm total motility >55% (56.0% and 64.0%) and progressive motility ~35% (35% and 40%), both frozen with Tris–yolk egg. Plasma and acrosome membrane integrity decreased and percentage of sperm defects increased post-thawing. We concluded that is possible to freeze spermatozoa from maned wolves using semen collection and processing methods applied for domestic dogs.Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)University of Franca (UNIFRAN)Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)Universidade Estadual Paulista (UNESP)University of Franca (UNIFRAN)Teodoro, Laura O. [UNESP]Camargo, Laiza S. [UNESP]Scheeren, Verônica F. C. [UNESP]Freitas-Dell'Aqua, Camila P. [UNESP]Papa, Frederico O. [UNESP]Honsho, Cristiane S.Souza, Fabiana F. [UNESP]2022-04-29T08:32:10Z2022-04-29T08:32:10Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1111/rda.13999Reproduction in Domestic Animals.1439-05310936-6768http://hdl.handle.net/11449/22937510.1111/rda.139992-s2.0-85113169581Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengReproduction in Domestic Animalsinfo:eu-repo/semantics/openAccess2024-09-09T14:05:33Zoai:repositorio.unesp.br:11449/229375Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:05:33Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv First successful frozen semen of the maned wolf (Chrysocyon brachyurus)
title First successful frozen semen of the maned wolf (Chrysocyon brachyurus)
spellingShingle First successful frozen semen of the maned wolf (Chrysocyon brachyurus)
Teodoro, Laura O. [UNESP]
captive-wolf
cryopreservation
spermatozoa
title_short First successful frozen semen of the maned wolf (Chrysocyon brachyurus)
title_full First successful frozen semen of the maned wolf (Chrysocyon brachyurus)
title_fullStr First successful frozen semen of the maned wolf (Chrysocyon brachyurus)
title_full_unstemmed First successful frozen semen of the maned wolf (Chrysocyon brachyurus)
title_sort First successful frozen semen of the maned wolf (Chrysocyon brachyurus)
author Teodoro, Laura O. [UNESP]
author_facet Teodoro, Laura O. [UNESP]
Camargo, Laiza S. [UNESP]
Scheeren, Verônica F. C. [UNESP]
Freitas-Dell'Aqua, Camila P. [UNESP]
Papa, Frederico O. [UNESP]
Honsho, Cristiane S.
Souza, Fabiana F. [UNESP]
author_role author
author2 Camargo, Laiza S. [UNESP]
Scheeren, Verônica F. C. [UNESP]
Freitas-Dell'Aqua, Camila P. [UNESP]
Papa, Frederico O. [UNESP]
Honsho, Cristiane S.
Souza, Fabiana F. [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
University of Franca (UNIFRAN)
dc.contributor.author.fl_str_mv Teodoro, Laura O. [UNESP]
Camargo, Laiza S. [UNESP]
Scheeren, Verônica F. C. [UNESP]
Freitas-Dell'Aqua, Camila P. [UNESP]
Papa, Frederico O. [UNESP]
Honsho, Cristiane S.
Souza, Fabiana F. [UNESP]
dc.subject.por.fl_str_mv captive-wolf
cryopreservation
spermatozoa
topic captive-wolf
cryopreservation
spermatozoa
description This study aimed to describe successful cryopreservation of sperm from maned wolves (Chrysocyon brachyurus). Three ejaculates from 2 maned wolves were collected by digital manipulation of the penis and evaluated subjectively, centrifuged and frozen in BotuCrio® (Botupharma, Botucatu, Brazil) or Tris–yolk egg extender. Spermatozoa were thawed at 37ºC/30s or 70ºC/4s and evaluated for kinetics, morphology, plasma and acrosome membrane integrity, mitochondrial potential, hydrogen peroxide, superoxide anion and lipid peroxidation. From 5 thawed samples, two had sperm total motility >55% (56.0% and 64.0%) and progressive motility ~35% (35% and 40%), both frozen with Tris–yolk egg. Plasma and acrosome membrane integrity decreased and percentage of sperm defects increased post-thawing. We concluded that is possible to freeze spermatozoa from maned wolves using semen collection and processing methods applied for domestic dogs.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
2022-04-29T08:32:10Z
2022-04-29T08:32:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1111/rda.13999
Reproduction in Domestic Animals.
1439-0531
0936-6768
http://hdl.handle.net/11449/229375
10.1111/rda.13999
2-s2.0-85113169581
url http://dx.doi.org/10.1111/rda.13999
http://hdl.handle.net/11449/229375
identifier_str_mv Reproduction in Domestic Animals.
1439-0531
0936-6768
10.1111/rda.13999
2-s2.0-85113169581
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Reproduction in Domestic Animals
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1813546595160948736

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