Complete replacement of the mitochondrial genotype in a Bos indicus calf reconstructed by nuclear transfer to a bos taurus oocyte
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.ncbi.nlm.nih.gov/pubmed/11333243 http://hdl.handle.net/11449/66523 |
Resumo: | Due to the exclusively maternal inheritance of mitochondria, mitochondrial genotypes can be coupled to a particular nuclear genotype by continuous mating of founder females and their female offspring to males of the desired nuclear genotype. However, backcrossing is a gradual procedure that, apart from being lengthy, cannot ascertain that genetic and epigenetic changes will modify the original nuclear genotype. Animal cloning by nuclear transfer using host ooplasm carrying polymorphic mitochondrial genomes allows, among other biotechnology applications, the coupling of nuclear and mitochondrial genotypes of diverse origin within a single generation. Previous attempts to use Bos taurus oocytes as hosts to transfer nuclei from unrelated species led to the development to the blastocyst stage but none supported gestation to term. Our aim in this study was to determine whether B. taurus oocytes support development of nuclei from the closely related B. indicus cattle and to examine the fate of their mitochondrial genotypes throughout development. We show that indicus:taurus reconstructed oocytes develop to the blastocyst stage and produce live offspring after transfer to surrogate cows. We also demonstrate that, in reconstructed embryos, donor cell-derived mitochondria undergo a stringent genetic drift during early development leading, in most cases, to a reduction or complete elimination of B. indicus mtDNA. These results demonstrate that cross-subspecies animal cloning is a viable approach both for matching diverse nuclear and cytoplasmic genes to create novel breeds of cattle and for rescuing closely related endangered cattle. |
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Complete replacement of the mitochondrial genotype in a Bos indicus calf reconstructed by nuclear transfer to a bos taurus oocyteanimal cellanimal experimentblastocystBos indicusBos tauruscattlecross breedingdevelopmentfemalegene constructgenotypemalematingmitochondrionmolecular cloningnonhumanoocytepriority journalAnimalsBase SequenceCattleCell NucleusDNA PrimersEmbryoGenotypeMitochondriaOocytesDue to the exclusively maternal inheritance of mitochondria, mitochondrial genotypes can be coupled to a particular nuclear genotype by continuous mating of founder females and their female offspring to males of the desired nuclear genotype. However, backcrossing is a gradual procedure that, apart from being lengthy, cannot ascertain that genetic and epigenetic changes will modify the original nuclear genotype. Animal cloning by nuclear transfer using host ooplasm carrying polymorphic mitochondrial genomes allows, among other biotechnology applications, the coupling of nuclear and mitochondrial genotypes of diverse origin within a single generation. Previous attempts to use Bos taurus oocytes as hosts to transfer nuclei from unrelated species led to the development to the blastocyst stage but none supported gestation to term. Our aim in this study was to determine whether B. taurus oocytes support development of nuclei from the closely related B. indicus cattle and to examine the fate of their mitochondrial genotypes throughout development. We show that indicus:taurus reconstructed oocytes develop to the blastocyst stage and produce live offspring after transfer to surrogate cows. We also demonstrate that, in reconstructed embryos, donor cell-derived mitochondria undergo a stringent genetic drift during early development leading, in most cases, to a reduction or complete elimination of B. indicus mtDNA. These results demonstrate that cross-subspecies animal cloning is a viable approach both for matching diverse nuclear and cytoplasmic genes to create novel breeds of cattle and for rescuing closely related endangered cattle.Centre de Recherche en Reproduction Animale Faculté de Médecine Vétérinaire Université de Montréal, Saint-Hyacinthe, Que. J2S 7C6Departamento de Genética Faculdade de Medicina-USP, Ribeirão Preto, SP 14049-900Departamento de Reprodução Animal Faculdade de Ciências Agrárias e Veterinárias UNESP, Jaboticabal, SP 14884-900Centre de Recherche en Reproduction Animale Faculté de Médecine Vétérinaire Université de Montréal, 3200, rue Sicotte CP 5000, Saint-Hyacinthe, Que. J2S 7C6Departamento de Reprodução Animal Faculdade de Ciências Agrárias e Veterinárias UNESP, Jaboticabal, SP 14884-900Université de MontréalUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Meirelles, Flávio V.Bordignon, VilceuWatanabe, YedaWatanabe, Michelle [UNESP]Dayan, André [UNESP]Lôbo, Raysildo B.Garcia, Joaquim M. [UNESP]Smith, Lawrence C.2014-05-27T11:20:16Z2014-05-27T11:20:16Z2001-05-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article351-356application/pdfhttp://www.ncbi.nlm.nih.gov/pubmed/11333243Genetics, v. 158, n. 1, p. 351-356, 2001.0016-6731http://hdl.handle.net/11449/66523WOS:0001686370000312-s2.0-00350247122-s2.0-0035024712.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics4.0753,484info:eu-repo/semantics/openAccess2023-10-24T06:11:50Zoai:repositorio.unesp.br:11449/66523Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-24T06:11:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Complete replacement of the mitochondrial genotype in a Bos indicus calf reconstructed by nuclear transfer to a bos taurus oocyte |
title |
Complete replacement of the mitochondrial genotype in a Bos indicus calf reconstructed by nuclear transfer to a bos taurus oocyte |
spellingShingle |
Complete replacement of the mitochondrial genotype in a Bos indicus calf reconstructed by nuclear transfer to a bos taurus oocyte Meirelles, Flávio V. animal cell animal experiment blastocyst Bos indicus Bos taurus cattle cross breeding development female gene construct genotype male mating mitochondrion molecular cloning nonhuman oocyte priority journal Animals Base Sequence Cattle Cell Nucleus DNA Primers Embryo Genotype Mitochondria Oocytes |
title_short |
Complete replacement of the mitochondrial genotype in a Bos indicus calf reconstructed by nuclear transfer to a bos taurus oocyte |
title_full |
Complete replacement of the mitochondrial genotype in a Bos indicus calf reconstructed by nuclear transfer to a bos taurus oocyte |
title_fullStr |
Complete replacement of the mitochondrial genotype in a Bos indicus calf reconstructed by nuclear transfer to a bos taurus oocyte |
title_full_unstemmed |
Complete replacement of the mitochondrial genotype in a Bos indicus calf reconstructed by nuclear transfer to a bos taurus oocyte |
title_sort |
Complete replacement of the mitochondrial genotype in a Bos indicus calf reconstructed by nuclear transfer to a bos taurus oocyte |
author |
Meirelles, Flávio V. |
author_facet |
Meirelles, Flávio V. Bordignon, Vilceu Watanabe, Yeda Watanabe, Michelle [UNESP] Dayan, André [UNESP] Lôbo, Raysildo B. Garcia, Joaquim M. [UNESP] Smith, Lawrence C. |
author_role |
author |
author2 |
Bordignon, Vilceu Watanabe, Yeda Watanabe, Michelle [UNESP] Dayan, André [UNESP] Lôbo, Raysildo B. Garcia, Joaquim M. [UNESP] Smith, Lawrence C. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Université de Montréal Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Meirelles, Flávio V. Bordignon, Vilceu Watanabe, Yeda Watanabe, Michelle [UNESP] Dayan, André [UNESP] Lôbo, Raysildo B. Garcia, Joaquim M. [UNESP] Smith, Lawrence C. |
dc.subject.por.fl_str_mv |
animal cell animal experiment blastocyst Bos indicus Bos taurus cattle cross breeding development female gene construct genotype male mating mitochondrion molecular cloning nonhuman oocyte priority journal Animals Base Sequence Cattle Cell Nucleus DNA Primers Embryo Genotype Mitochondria Oocytes |
topic |
animal cell animal experiment blastocyst Bos indicus Bos taurus cattle cross breeding development female gene construct genotype male mating mitochondrion molecular cloning nonhuman oocyte priority journal Animals Base Sequence Cattle Cell Nucleus DNA Primers Embryo Genotype Mitochondria Oocytes |
description |
Due to the exclusively maternal inheritance of mitochondria, mitochondrial genotypes can be coupled to a particular nuclear genotype by continuous mating of founder females and their female offspring to males of the desired nuclear genotype. However, backcrossing is a gradual procedure that, apart from being lengthy, cannot ascertain that genetic and epigenetic changes will modify the original nuclear genotype. Animal cloning by nuclear transfer using host ooplasm carrying polymorphic mitochondrial genomes allows, among other biotechnology applications, the coupling of nuclear and mitochondrial genotypes of diverse origin within a single generation. Previous attempts to use Bos taurus oocytes as hosts to transfer nuclei from unrelated species led to the development to the blastocyst stage but none supported gestation to term. Our aim in this study was to determine whether B. taurus oocytes support development of nuclei from the closely related B. indicus cattle and to examine the fate of their mitochondrial genotypes throughout development. We show that indicus:taurus reconstructed oocytes develop to the blastocyst stage and produce live offspring after transfer to surrogate cows. We also demonstrate that, in reconstructed embryos, donor cell-derived mitochondria undergo a stringent genetic drift during early development leading, in most cases, to a reduction or complete elimination of B. indicus mtDNA. These results demonstrate that cross-subspecies animal cloning is a viable approach both for matching diverse nuclear and cytoplasmic genes to create novel breeds of cattle and for rescuing closely related endangered cattle. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-05-22 2014-05-27T11:20:16Z 2014-05-27T11:20:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.ncbi.nlm.nih.gov/pubmed/11333243 Genetics, v. 158, n. 1, p. 351-356, 2001. 0016-6731 http://hdl.handle.net/11449/66523 WOS:000168637000031 2-s2.0-0035024712 2-s2.0-0035024712.pdf |
url |
http://www.ncbi.nlm.nih.gov/pubmed/11333243 http://hdl.handle.net/11449/66523 |
identifier_str_mv |
Genetics, v. 158, n. 1, p. 351-356, 2001. 0016-6731 WOS:000168637000031 2-s2.0-0035024712 2-s2.0-0035024712.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics 4.075 3,484 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
351-356 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964684298747904 |