Severity of prepregnancy diabetes on the fetal malformations and viability associated with early embryos in rats
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/biolre/ioaa151 http://hdl.handle.net/11449/206798 |
Resumo: | Preexisting/pregestational diabetes enhances the risk of birth defects. Several factors have been involved during the implantation process, such as cytokines (granulocyte-macrophage-colony-stimulating factor [GM-CSF]). The objective was to evaluate the effects of two levels of diabetes on the redox status of preimplantation embryos during the implantation process to comprehend how both are involved in embryo and fetal viability against maternal diabetes. Female Sprague-Dawley rats received streptozotocin at birth (mild diabetes [MD]) or at adulthood (severe diabetes [SD]) to obtain two experimental diabetes intensities. After confirming the diabetic status, the nondiabetic and diabetic groups were mated around day 110 of life. At gestational day (GD) 21, fetuses were assessed for viability and malformations and ovaries for embryo loss before implantation. Other pregnant nondiabetic and diabetic rats were sacrificed at GD2-4 for maternal and preimplantation embryo oxidative stress markers, maternal serum insulin, uterine fluid GM-CSF, and preimplantation embryo morphological analysis. MD and SD caused abnormal redox levels, lower GM-CSF and insulin levels during the preimplantation period, and embryonic loss before implantation. SD caused lower fetal viability and higher fetal malformation percentages at GD21. The SD dam-derived preimplantation embryos presented lower glutathione levels and higher thiobarbituric acid reactive substances concentration at GD3 and an increased frequency of abnormal preimplantation embryos at GD4. In conclusion, preexisting diabetes leads to complications in the implantation process. Furthermore, maternal oxidative stress and other metabolic changes alter the redox state and morphological structure of preimplantation embryos, contributing to damaged growth and development in late pregnancy. |
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Severity of prepregnancy diabetes on the fetal malformations and viability associated with early embryos in ratsfetushyperglycemiaoxidative stresspregestationalratPreexisting/pregestational diabetes enhances the risk of birth defects. Several factors have been involved during the implantation process, such as cytokines (granulocyte-macrophage-colony-stimulating factor [GM-CSF]). The objective was to evaluate the effects of two levels of diabetes on the redox status of preimplantation embryos during the implantation process to comprehend how both are involved in embryo and fetal viability against maternal diabetes. Female Sprague-Dawley rats received streptozotocin at birth (mild diabetes [MD]) or at adulthood (severe diabetes [SD]) to obtain two experimental diabetes intensities. After confirming the diabetic status, the nondiabetic and diabetic groups were mated around day 110 of life. At gestational day (GD) 21, fetuses were assessed for viability and malformations and ovaries for embryo loss before implantation. Other pregnant nondiabetic and diabetic rats were sacrificed at GD2-4 for maternal and preimplantation embryo oxidative stress markers, maternal serum insulin, uterine fluid GM-CSF, and preimplantation embryo morphological analysis. MD and SD caused abnormal redox levels, lower GM-CSF and insulin levels during the preimplantation period, and embryonic loss before implantation. SD caused lower fetal viability and higher fetal malformation percentages at GD21. The SD dam-derived preimplantation embryos presented lower glutathione levels and higher thiobarbituric acid reactive substances concentration at GD3 and an increased frequency of abnormal preimplantation embryos at GD4. In conclusion, preexisting diabetes leads to complications in the implantation process. Furthermore, maternal oxidative stress and other metabolic changes alter the redox state and morphological structure of preimplantation embryos, contributing to damaged growth and development in late pregnancy.Laboratory of Experimental Research on Gynecology and Obstetrics Postgrad. Course on Tocogynecology Botucatu Med. Sch. S. Paulo State Univ. (Unesp) Botucatu S. Paulo StateLaboratory of Experimental Research on Gynecology and Obstetrics Postgrad. Course on Tocogynecology Botucatu Med. Sch. S. Paulo State Univ. (Unesp) Botucatu S. Paulo StateUniversidade Estadual Paulista (Unesp)Bueno, Aline [UNESP]Sinzato, Yuri Karen [UNESP]Volpato, Gustavo Tadeu [UNESP]Gallego, Franciane Quintanilha [UNESP]Perecin, Felipe [UNESP]Rodrigues, Tiago [UNESP]Damasceno, Débora Cristina [UNESP]2021-06-25T10:44:03Z2021-06-25T10:44:03Z2020-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article938-950http://dx.doi.org/10.1093/biolre/ioaa151Biology of Reproduction, v. 103, n. 5, p. 938-950, 2020.1529-72680006-3363http://hdl.handle.net/11449/20679810.1093/biolre/ioaa1512-s2.0-85095673946Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiology of Reproductioninfo:eu-repo/semantics/openAccess2021-10-23T15:25:04Zoai:repositorio.unesp.br:11449/206798Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T15:25:04Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Severity of prepregnancy diabetes on the fetal malformations and viability associated with early embryos in rats |
title |
Severity of prepregnancy diabetes on the fetal malformations and viability associated with early embryos in rats |
spellingShingle |
Severity of prepregnancy diabetes on the fetal malformations and viability associated with early embryos in rats Bueno, Aline [UNESP] fetus hyperglycemia oxidative stress pregestational rat |
title_short |
Severity of prepregnancy diabetes on the fetal malformations and viability associated with early embryos in rats |
title_full |
Severity of prepregnancy diabetes on the fetal malformations and viability associated with early embryos in rats |
title_fullStr |
Severity of prepregnancy diabetes on the fetal malformations and viability associated with early embryos in rats |
title_full_unstemmed |
Severity of prepregnancy diabetes on the fetal malformations and viability associated with early embryos in rats |
title_sort |
Severity of prepregnancy diabetes on the fetal malformations and viability associated with early embryos in rats |
author |
Bueno, Aline [UNESP] |
author_facet |
Bueno, Aline [UNESP] Sinzato, Yuri Karen [UNESP] Volpato, Gustavo Tadeu [UNESP] Gallego, Franciane Quintanilha [UNESP] Perecin, Felipe [UNESP] Rodrigues, Tiago [UNESP] Damasceno, Débora Cristina [UNESP] |
author_role |
author |
author2 |
Sinzato, Yuri Karen [UNESP] Volpato, Gustavo Tadeu [UNESP] Gallego, Franciane Quintanilha [UNESP] Perecin, Felipe [UNESP] Rodrigues, Tiago [UNESP] Damasceno, Débora Cristina [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Bueno, Aline [UNESP] Sinzato, Yuri Karen [UNESP] Volpato, Gustavo Tadeu [UNESP] Gallego, Franciane Quintanilha [UNESP] Perecin, Felipe [UNESP] Rodrigues, Tiago [UNESP] Damasceno, Débora Cristina [UNESP] |
dc.subject.por.fl_str_mv |
fetus hyperglycemia oxidative stress pregestational rat |
topic |
fetus hyperglycemia oxidative stress pregestational rat |
description |
Preexisting/pregestational diabetes enhances the risk of birth defects. Several factors have been involved during the implantation process, such as cytokines (granulocyte-macrophage-colony-stimulating factor [GM-CSF]). The objective was to evaluate the effects of two levels of diabetes on the redox status of preimplantation embryos during the implantation process to comprehend how both are involved in embryo and fetal viability against maternal diabetes. Female Sprague-Dawley rats received streptozotocin at birth (mild diabetes [MD]) or at adulthood (severe diabetes [SD]) to obtain two experimental diabetes intensities. After confirming the diabetic status, the nondiabetic and diabetic groups were mated around day 110 of life. At gestational day (GD) 21, fetuses were assessed for viability and malformations and ovaries for embryo loss before implantation. Other pregnant nondiabetic and diabetic rats were sacrificed at GD2-4 for maternal and preimplantation embryo oxidative stress markers, maternal serum insulin, uterine fluid GM-CSF, and preimplantation embryo morphological analysis. MD and SD caused abnormal redox levels, lower GM-CSF and insulin levels during the preimplantation period, and embryonic loss before implantation. SD caused lower fetal viability and higher fetal malformation percentages at GD21. The SD dam-derived preimplantation embryos presented lower glutathione levels and higher thiobarbituric acid reactive substances concentration at GD3 and an increased frequency of abnormal preimplantation embryos at GD4. In conclusion, preexisting diabetes leads to complications in the implantation process. Furthermore, maternal oxidative stress and other metabolic changes alter the redox state and morphological structure of preimplantation embryos, contributing to damaged growth and development in late pregnancy. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-01 2021-06-25T10:44:03Z 2021-06-25T10:44:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/biolre/ioaa151 Biology of Reproduction, v. 103, n. 5, p. 938-950, 2020. 1529-7268 0006-3363 http://hdl.handle.net/11449/206798 10.1093/biolre/ioaa151 2-s2.0-85095673946 |
url |
http://dx.doi.org/10.1093/biolre/ioaa151 http://hdl.handle.net/11449/206798 |
identifier_str_mv |
Biology of Reproduction, v. 103, n. 5, p. 938-950, 2020. 1529-7268 0006-3363 10.1093/biolre/ioaa151 2-s2.0-85095673946 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biology of Reproduction |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
938-950 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964550393495552 |