Developmental competence of domestic cat vitrified oocytes in 3D enriched culture conditions
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ani9060329 http://hdl.handle.net/11449/187943 |
Resumo: | Cryoinjuries severely affect the competence of vitrified oocytes (VOs) to develop into embryos after warming. The use of culture conditions that provide physical and chemical support and resemble the in vivo microenvironment in which oocytes develop, such as 3D scaffolds and coculture systems, might be useful to improve VOs outcomes. In this study, an enriched culture system of 3D barium alginate microcapsules was employed for the in vitro embryo production of domestic cat VOs. Cryotop vitrified-warmed oocytes were in vitro matured for 24 h in the 3D system with or without fresh cumulus-oocyte complexes (COCs) in coculture, whereas a control group of VOs was cultured in traditional 2D microdrops of medium. After in vitro fertilization, presumptive embryos were cultured in 3D or 2D systems according to the maturation conditions. Vitrified oocytes were able to mature and develop into embryos in 3D microcapsules (17.42 ± 11.83%) as well as in 2D microdrops (14.96 ± 8.80%), but the coculture with companion COCs in 3D resulted in similar proportions of VOs embryo development (18.39 ± 16.67%; p = 1.00), although COCs presence allowed for blastocyst formation (0.95 ± 2.52%). In conclusion, embryos until late developmental stages were obtained from cat VOs, and 3D microcapsules were comparable to 2D microdrops, but improvements in post-warming conditions are still needed. |
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Developmental competence of domestic cat vitrified oocytes in 3D enriched culture conditionsAlginate microcapsuleBlastocystCryopreservationFelineIn vitro cultureThree-dimensionalCryoinjuries severely affect the competence of vitrified oocytes (VOs) to develop into embryos after warming. The use of culture conditions that provide physical and chemical support and resemble the in vivo microenvironment in which oocytes develop, such as 3D scaffolds and coculture systems, might be useful to improve VOs outcomes. In this study, an enriched culture system of 3D barium alginate microcapsules was employed for the in vitro embryo production of domestic cat VOs. Cryotop vitrified-warmed oocytes were in vitro matured for 24 h in the 3D system with or without fresh cumulus-oocyte complexes (COCs) in coculture, whereas a control group of VOs was cultured in traditional 2D microdrops of medium. After in vitro fertilization, presumptive embryos were cultured in 3D or 2D systems according to the maturation conditions. Vitrified oocytes were able to mature and develop into embryos in 3D microcapsules (17.42 ± 11.83%) as well as in 2D microdrops (14.96 ± 8.80%), but the coculture with companion COCs in 3D resulted in similar proportions of VOs embryo development (18.39 ± 16.67%; p = 1.00), although COCs presence allowed for blastocyst formation (0.95 ± 2.52%). In conclusion, embryos until late developmental stages were obtained from cat VOs, and 3D microcapsules were comparable to 2D microdrops, but improvements in post-warming conditions are still needed.Dipartimento di Scienze Veterinarie per la Salute la Produzione Animale e la Sicurezza Alimentare “Carlo Cantoni” Università degli Studi di Milano, Via Celoria, 10Departamento de Medicina Veterinária Preventiva e Reprodução Animal Universidade Estadual Paulista (UNESP), Via de Acesso Prof. Paulo Donato Castellane s/nDepartamento de Medicina Veterinária Preventiva e Reprodução Animal Universidade Estadual Paulista (UNESP), Via de Acesso Prof. Paulo Donato Castellane s/nUniversità degli Studi di MilanoUniversidade Estadual Paulista (Unesp)Colombo, MartinaMorselli, Maria GiorgiaTavares, Mariana Riboli [UNESP]Apparicio, Maricy [UNESP]Luvoni, Gaia Cecilia2019-10-06T15:52:06Z2019-10-06T15:52:06Z2019-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ani9060329Animals, v. 9, n. 6, 2019.2076-2615http://hdl.handle.net/11449/18794310.3390/ani90603292-s2.0-85070479261Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimalsinfo:eu-repo/semantics/openAccess2021-10-23T02:53:58Zoai:repositorio.unesp.br:11449/187943Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T02:53:58Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Developmental competence of domestic cat vitrified oocytes in 3D enriched culture conditions |
title |
Developmental competence of domestic cat vitrified oocytes in 3D enriched culture conditions |
spellingShingle |
Developmental competence of domestic cat vitrified oocytes in 3D enriched culture conditions Colombo, Martina Alginate microcapsule Blastocyst Cryopreservation Feline In vitro culture Three-dimensional |
title_short |
Developmental competence of domestic cat vitrified oocytes in 3D enriched culture conditions |
title_full |
Developmental competence of domestic cat vitrified oocytes in 3D enriched culture conditions |
title_fullStr |
Developmental competence of domestic cat vitrified oocytes in 3D enriched culture conditions |
title_full_unstemmed |
Developmental competence of domestic cat vitrified oocytes in 3D enriched culture conditions |
title_sort |
Developmental competence of domestic cat vitrified oocytes in 3D enriched culture conditions |
author |
Colombo, Martina |
author_facet |
Colombo, Martina Morselli, Maria Giorgia Tavares, Mariana Riboli [UNESP] Apparicio, Maricy [UNESP] Luvoni, Gaia Cecilia |
author_role |
author |
author2 |
Morselli, Maria Giorgia Tavares, Mariana Riboli [UNESP] Apparicio, Maricy [UNESP] Luvoni, Gaia Cecilia |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Università degli Studi di Milano Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Colombo, Martina Morselli, Maria Giorgia Tavares, Mariana Riboli [UNESP] Apparicio, Maricy [UNESP] Luvoni, Gaia Cecilia |
dc.subject.por.fl_str_mv |
Alginate microcapsule Blastocyst Cryopreservation Feline In vitro culture Three-dimensional |
topic |
Alginate microcapsule Blastocyst Cryopreservation Feline In vitro culture Three-dimensional |
description |
Cryoinjuries severely affect the competence of vitrified oocytes (VOs) to develop into embryos after warming. The use of culture conditions that provide physical and chemical support and resemble the in vivo microenvironment in which oocytes develop, such as 3D scaffolds and coculture systems, might be useful to improve VOs outcomes. In this study, an enriched culture system of 3D barium alginate microcapsules was employed for the in vitro embryo production of domestic cat VOs. Cryotop vitrified-warmed oocytes were in vitro matured for 24 h in the 3D system with or without fresh cumulus-oocyte complexes (COCs) in coculture, whereas a control group of VOs was cultured in traditional 2D microdrops of medium. After in vitro fertilization, presumptive embryos were cultured in 3D or 2D systems according to the maturation conditions. Vitrified oocytes were able to mature and develop into embryos in 3D microcapsules (17.42 ± 11.83%) as well as in 2D microdrops (14.96 ± 8.80%), but the coculture with companion COCs in 3D resulted in similar proportions of VOs embryo development (18.39 ± 16.67%; p = 1.00), although COCs presence allowed for blastocyst formation (0.95 ± 2.52%). In conclusion, embryos until late developmental stages were obtained from cat VOs, and 3D microcapsules were comparable to 2D microdrops, but improvements in post-warming conditions are still needed. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T15:52:06Z 2019-10-06T15:52:06Z 2019-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ani9060329 Animals, v. 9, n. 6, 2019. 2076-2615 http://hdl.handle.net/11449/187943 10.3390/ani9060329 2-s2.0-85070479261 |
url |
http://dx.doi.org/10.3390/ani9060329 http://hdl.handle.net/11449/187943 |
identifier_str_mv |
Animals, v. 9, n. 6, 2019. 2076-2615 10.3390/ani9060329 2-s2.0-85070479261 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animals |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964787195510784 |