Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms

Detalhes bibliográficos
Autor(a) principal: Koga-Ito, Cristiane Yumi [UNESP]
Data de Publicação: 2021
Outros Autores: Leite, Lady Daiane Pereira, Oliveira, Maria Alcionéia Carvalho de, Vegian, Mariana Raquel da Cruz [UNESP], Sampaio, Aline da Graça [UNESP], Nishime, Thalita Mayumi Castaldelli [UNESP], Kostov, Konstantin Georgiev
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/215986
Resumo: : The increasing incidence of antifungal resistance represents a great challenge in the medical area and, for this reason, new therapeutic alternatives for the treatment of fungal infections are urgently required. Cold atmospheric plasma (CAP) has been proposed as a promising alternative technique for the treatment of superficial candidiasis, with inhibitory effect both in vitro and in vivo. However, little is known on the association of CAP with conventional antifungals. The aim of this study was to evaluate the effects of the association between CAP and conventional polyene antifungals on Candida albicans biofilms. C. albicans SC 5314 and a clinical isolate were used to grow 24 or 48 h biofilms, under standardized conditions. After that, the biofilms were exposed to nystatin, amphotericin B and CAP, separately or in combination. Different concentrations of the antifungals and sequences of treatment were evaluated to establish the most effective protocol. Biofilms viability after the treatments was compared to negative control. Data were compared by One-way ANOVA and post hoc Tukey (5%). The results demonstrate that 5 min exposure to CAP showed more effective antifungal effect on biofilms when compared to nystatin and amphotericin B. Additionally, it was detected that CAP showed similar (but smaller in magnitude) effects when applied in association with nystatin and amphotericin B at 40 µg/mL and 60 µg/mL. Therefore, it can be concluded that the application of CAP alone was more effective against C. albicans biofilms than in combination with conventional polyene antifungal agents.
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spelling Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilmsCandida albicansAntifungalCold plasma: The increasing incidence of antifungal resistance represents a great challenge in the medical area and, for this reason, new therapeutic alternatives for the treatment of fungal infections are urgently required. Cold atmospheric plasma (CAP) has been proposed as a promising alternative technique for the treatment of superficial candidiasis, with inhibitory effect both in vitro and in vivo. However, little is known on the association of CAP with conventional antifungals. The aim of this study was to evaluate the effects of the association between CAP and conventional polyene antifungals on Candida albicans biofilms. C. albicans SC 5314 and a clinical isolate were used to grow 24 or 48 h biofilms, under standardized conditions. After that, the biofilms were exposed to nystatin, amphotericin B and CAP, separately or in combination. Different concentrations of the antifungals and sequences of treatment were evaluated to establish the most effective protocol. Biofilms viability after the treatments was compared to negative control. Data were compared by One-way ANOVA and post hoc Tukey (5%). The results demonstrate that 5 min exposure to CAP showed more effective antifungal effect on biofilms when compared to nystatin and amphotericin B. Additionally, it was detected that CAP showed similar (but smaller in magnitude) effects when applied in association with nystatin and amphotericin B at 40 µg/mL and 60 µg/mL. Therefore, it can be concluded that the application of CAP alone was more effective against C. albicans biofilms than in combination with conventional polyene antifungal agents.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Science Applied to Oral Health, Graduate Program of Institute of Science and Technology, São Paulo State University (Unesp), São José dos CamposDepartment of Environment Engineering, Institute of Science and Technology, São Paulo State University (Unesp), São José dos CamposOral Biopathology, Graduate Program of Institute of Science and Technology, São Paulo State University (Unesp), São José dos CamposLeibniz Institute for Plasma Science and TechnologyDepartment of Physics, Guaratinguetá Faculty of Engineering, São Paulo State University (Unesp), GuaratinguetáFAPESP: 2019/05856-7CAPES: 001CNPq: 308127/2018-80MDPIUniversidade Estadual Paulista (Unesp)Koga-Ito, Cristiane Yumi [UNESP]Leite, Lady Daiane PereiraOliveira, Maria Alcionéia Carvalho deVegian, Mariana Raquel da Cruz [UNESP]Sampaio, Aline da Graça [UNESP]Nishime, Thalita Mayumi Castaldelli [UNESP]Kostov, Konstantin Georgiev2022-01-20T17:48:24Z2022-01-20T17:48:24Z2021-09-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfLEITE, L. D. P.; OLIVEIRA, M. A. C.; VEGIAN, M. R. C.; SAMPAIO, A. G.; NISHIME, T. M. C.; KOSTOV, K. G.; KOGA-ITO, C. Y. Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms. Molecules, v. 26, n. 19, p. 5815, 2021.1420-3049http://hdl.handle.net/11449/21598665435631614034210000-0002-2416-2173enghttps://doi.org/10.3390/molecules26195815Moleculesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESP2023-10-10T06:02:37Zoai:repositorio.unesp.br:11449/215986Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:29:51.130044Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms
title Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms
spellingShingle Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms
Koga-Ito, Cristiane Yumi [UNESP]
Candida albicans
Antifungal
Cold plasma
title_short Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms
title_full Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms
title_fullStr Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms
title_full_unstemmed Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms
title_sort Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms
author Koga-Ito, Cristiane Yumi [UNESP]
author_facet Koga-Ito, Cristiane Yumi [UNESP]
Leite, Lady Daiane Pereira
Oliveira, Maria Alcionéia Carvalho de
Vegian, Mariana Raquel da Cruz [UNESP]
Sampaio, Aline da Graça [UNESP]
Nishime, Thalita Mayumi Castaldelli [UNESP]
Kostov, Konstantin Georgiev
author_role author
author2 Leite, Lady Daiane Pereira
Oliveira, Maria Alcionéia Carvalho de
Vegian, Mariana Raquel da Cruz [UNESP]
Sampaio, Aline da Graça [UNESP]
Nishime, Thalita Mayumi Castaldelli [UNESP]
Kostov, Konstantin Georgiev
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Koga-Ito, Cristiane Yumi [UNESP]
Leite, Lady Daiane Pereira
Oliveira, Maria Alcionéia Carvalho de
Vegian, Mariana Raquel da Cruz [UNESP]
Sampaio, Aline da Graça [UNESP]
Nishime, Thalita Mayumi Castaldelli [UNESP]
Kostov, Konstantin Georgiev
dc.subject.por.fl_str_mv Candida albicans
Antifungal
Cold plasma
topic Candida albicans
Antifungal
Cold plasma
description : The increasing incidence of antifungal resistance represents a great challenge in the medical area and, for this reason, new therapeutic alternatives for the treatment of fungal infections are urgently required. Cold atmospheric plasma (CAP) has been proposed as a promising alternative technique for the treatment of superficial candidiasis, with inhibitory effect both in vitro and in vivo. However, little is known on the association of CAP with conventional antifungals. The aim of this study was to evaluate the effects of the association between CAP and conventional polyene antifungals on Candida albicans biofilms. C. albicans SC 5314 and a clinical isolate were used to grow 24 or 48 h biofilms, under standardized conditions. After that, the biofilms were exposed to nystatin, amphotericin B and CAP, separately or in combination. Different concentrations of the antifungals and sequences of treatment were evaluated to establish the most effective protocol. Biofilms viability after the treatments was compared to negative control. Data were compared by One-way ANOVA and post hoc Tukey (5%). The results demonstrate that 5 min exposure to CAP showed more effective antifungal effect on biofilms when compared to nystatin and amphotericin B. Additionally, it was detected that CAP showed similar (but smaller in magnitude) effects when applied in association with nystatin and amphotericin B at 40 µg/mL and 60 µg/mL. Therefore, it can be concluded that the application of CAP alone was more effective against C. albicans biofilms than in combination with conventional polyene antifungal agents.
publishDate 2021
dc.date.none.fl_str_mv 2021-09-25
2022-01-20T17:48:24Z
2022-01-20T17:48:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv LEITE, L. D. P.; OLIVEIRA, M. A. C.; VEGIAN, M. R. C.; SAMPAIO, A. G.; NISHIME, T. M. C.; KOSTOV, K. G.; KOGA-ITO, C. Y. Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms. Molecules, v. 26, n. 19, p. 5815, 2021.
1420-3049
http://hdl.handle.net/11449/215986
6543563161403421
0000-0002-2416-2173
identifier_str_mv LEITE, L. D. P.; OLIVEIRA, M. A. C.; VEGIAN, M. R. C.; SAMPAIO, A. G.; NISHIME, T. M. C.; KOSTOV, K. G.; KOGA-ITO, C. Y. Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms. Molecules, v. 26, n. 19, p. 5815, 2021.
1420-3049
6543563161403421
0000-0002-2416-2173
url http://hdl.handle.net/11449/215986
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://doi.org/10.3390/molecules26195815
Molecules
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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