Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/215986 |
Resumo: | : The increasing incidence of antifungal resistance represents a great challenge in the medical area and, for this reason, new therapeutic alternatives for the treatment of fungal infections are urgently required. Cold atmospheric plasma (CAP) has been proposed as a promising alternative technique for the treatment of superficial candidiasis, with inhibitory effect both in vitro and in vivo. However, little is known on the association of CAP with conventional antifungals. The aim of this study was to evaluate the effects of the association between CAP and conventional polyene antifungals on Candida albicans biofilms. C. albicans SC 5314 and a clinical isolate were used to grow 24 or 48 h biofilms, under standardized conditions. After that, the biofilms were exposed to nystatin, amphotericin B and CAP, separately or in combination. Different concentrations of the antifungals and sequences of treatment were evaluated to establish the most effective protocol. Biofilms viability after the treatments was compared to negative control. Data were compared by One-way ANOVA and post hoc Tukey (5%). The results demonstrate that 5 min exposure to CAP showed more effective antifungal effect on biofilms when compared to nystatin and amphotericin B. Additionally, it was detected that CAP showed similar (but smaller in magnitude) effects when applied in association with nystatin and amphotericin B at 40 µg/mL and 60 µg/mL. Therefore, it can be concluded that the application of CAP alone was more effective against C. albicans biofilms than in combination with conventional polyene antifungal agents. |
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Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilmsCandida albicansAntifungalCold plasma: The increasing incidence of antifungal resistance represents a great challenge in the medical area and, for this reason, new therapeutic alternatives for the treatment of fungal infections are urgently required. Cold atmospheric plasma (CAP) has been proposed as a promising alternative technique for the treatment of superficial candidiasis, with inhibitory effect both in vitro and in vivo. However, little is known on the association of CAP with conventional antifungals. The aim of this study was to evaluate the effects of the association between CAP and conventional polyene antifungals on Candida albicans biofilms. C. albicans SC 5314 and a clinical isolate were used to grow 24 or 48 h biofilms, under standardized conditions. After that, the biofilms were exposed to nystatin, amphotericin B and CAP, separately or in combination. Different concentrations of the antifungals and sequences of treatment were evaluated to establish the most effective protocol. Biofilms viability after the treatments was compared to negative control. Data were compared by One-way ANOVA and post hoc Tukey (5%). The results demonstrate that 5 min exposure to CAP showed more effective antifungal effect on biofilms when compared to nystatin and amphotericin B. Additionally, it was detected that CAP showed similar (but smaller in magnitude) effects when applied in association with nystatin and amphotericin B at 40 µg/mL and 60 µg/mL. Therefore, it can be concluded that the application of CAP alone was more effective against C. albicans biofilms than in combination with conventional polyene antifungal agents.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Science Applied to Oral Health, Graduate Program of Institute of Science and Technology, São Paulo State University (Unesp), São José dos CamposDepartment of Environment Engineering, Institute of Science and Technology, São Paulo State University (Unesp), São José dos CamposOral Biopathology, Graduate Program of Institute of Science and Technology, São Paulo State University (Unesp), São José dos CamposLeibniz Institute for Plasma Science and TechnologyDepartment of Physics, Guaratinguetá Faculty of Engineering, São Paulo State University (Unesp), GuaratinguetáFAPESP: 2019/05856-7CAPES: 001CNPq: 308127/2018-80MDPIUniversidade Estadual Paulista (Unesp)Koga-Ito, Cristiane Yumi [UNESP]Leite, Lady Daiane PereiraOliveira, Maria Alcionéia Carvalho deVegian, Mariana Raquel da Cruz [UNESP]Sampaio, Aline da Graça [UNESP]Nishime, Thalita Mayumi Castaldelli [UNESP]Kostov, Konstantin Georgiev2022-01-20T17:48:24Z2022-01-20T17:48:24Z2021-09-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfLEITE, L. D. P.; OLIVEIRA, M. A. C.; VEGIAN, M. R. C.; SAMPAIO, A. G.; NISHIME, T. M. C.; KOSTOV, K. G.; KOGA-ITO, C. Y. Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms. Molecules, v. 26, n. 19, p. 5815, 2021.1420-3049http://hdl.handle.net/11449/21598665435631614034210000-0002-2416-2173enghttps://doi.org/10.3390/molecules26195815Moleculesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESP2023-10-10T06:02:37Zoai:repositorio.unesp.br:11449/215986Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:29:51.130044Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms |
title |
Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms |
spellingShingle |
Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms Koga-Ito, Cristiane Yumi [UNESP] Candida albicans Antifungal Cold plasma |
title_short |
Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms |
title_full |
Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms |
title_fullStr |
Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms |
title_full_unstemmed |
Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms |
title_sort |
Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms |
author |
Koga-Ito, Cristiane Yumi [UNESP] |
author_facet |
Koga-Ito, Cristiane Yumi [UNESP] Leite, Lady Daiane Pereira Oliveira, Maria Alcionéia Carvalho de Vegian, Mariana Raquel da Cruz [UNESP] Sampaio, Aline da Graça [UNESP] Nishime, Thalita Mayumi Castaldelli [UNESP] Kostov, Konstantin Georgiev |
author_role |
author |
author2 |
Leite, Lady Daiane Pereira Oliveira, Maria Alcionéia Carvalho de Vegian, Mariana Raquel da Cruz [UNESP] Sampaio, Aline da Graça [UNESP] Nishime, Thalita Mayumi Castaldelli [UNESP] Kostov, Konstantin Georgiev |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Koga-Ito, Cristiane Yumi [UNESP] Leite, Lady Daiane Pereira Oliveira, Maria Alcionéia Carvalho de Vegian, Mariana Raquel da Cruz [UNESP] Sampaio, Aline da Graça [UNESP] Nishime, Thalita Mayumi Castaldelli [UNESP] Kostov, Konstantin Georgiev |
dc.subject.por.fl_str_mv |
Candida albicans Antifungal Cold plasma |
topic |
Candida albicans Antifungal Cold plasma |
description |
: The increasing incidence of antifungal resistance represents a great challenge in the medical area and, for this reason, new therapeutic alternatives for the treatment of fungal infections are urgently required. Cold atmospheric plasma (CAP) has been proposed as a promising alternative technique for the treatment of superficial candidiasis, with inhibitory effect both in vitro and in vivo. However, little is known on the association of CAP with conventional antifungals. The aim of this study was to evaluate the effects of the association between CAP and conventional polyene antifungals on Candida albicans biofilms. C. albicans SC 5314 and a clinical isolate were used to grow 24 or 48 h biofilms, under standardized conditions. After that, the biofilms were exposed to nystatin, amphotericin B and CAP, separately or in combination. Different concentrations of the antifungals and sequences of treatment were evaluated to establish the most effective protocol. Biofilms viability after the treatments was compared to negative control. Data were compared by One-way ANOVA and post hoc Tukey (5%). The results demonstrate that 5 min exposure to CAP showed more effective antifungal effect on biofilms when compared to nystatin and amphotericin B. Additionally, it was detected that CAP showed similar (but smaller in magnitude) effects when applied in association with nystatin and amphotericin B at 40 µg/mL and 60 µg/mL. Therefore, it can be concluded that the application of CAP alone was more effective against C. albicans biofilms than in combination with conventional polyene antifungal agents. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-09-25 2022-01-20T17:48:24Z 2022-01-20T17:48:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
LEITE, L. D. P.; OLIVEIRA, M. A. C.; VEGIAN, M. R. C.; SAMPAIO, A. G.; NISHIME, T. M. C.; KOSTOV, K. G.; KOGA-ITO, C. Y. Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms. Molecules, v. 26, n. 19, p. 5815, 2021. 1420-3049 http://hdl.handle.net/11449/215986 6543563161403421 0000-0002-2416-2173 |
identifier_str_mv |
LEITE, L. D. P.; OLIVEIRA, M. A. C.; VEGIAN, M. R. C.; SAMPAIO, A. G.; NISHIME, T. M. C.; KOSTOV, K. G.; KOGA-ITO, C. Y. Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms. Molecules, v. 26, n. 19, p. 5815, 2021. 1420-3049 6543563161403421 0000-0002-2416-2173 |
url |
http://hdl.handle.net/11449/215986 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://doi.org/10.3390/molecules26195815 Molecules |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128368723361792 |