SAXS study of crotapotin at low pH

Detalhes bibliográficos
Autor(a) principal: Abrego, José Ramon Beltran [UNESP]
Data de Publicação: 1993
Outros Autores: Craievich, Aldo Felix, Mascarenhas, Yvonne Primerano, Laure, Carlos J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/S0006-3495(93)81395-X
http://hdl.handle.net/11449/33433
Resumo: The structure of crotapotin, a protein extracted from the venom of the Crotalus durissus terrificus, in solution at pH = 1.5, was studied by SAXS. The experimental results yield structural parameter values of the molecular radius of gyration R(g) = 13.6 angstrom, volume v = 16.2 x 10(3) angstrom3 and maximal dimension D(max) = 46 angstrom. The distance distribution function deduced from the scattering measurements is consistent with an overall molecular shape of an oblate ellipsoid of revolution with assymetry parameter nu = 0.45.
id UNSP_b3db825dccba98f741a1810652dba004
oai_identifier_str oai:repositorio.unesp.br:11449/33433
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling SAXS study of crotapotin at low pHThe structure of crotapotin, a protein extracted from the venom of the Crotalus durissus terrificus, in solution at pH = 1.5, was studied by SAXS. The experimental results yield structural parameter values of the molecular radius of gyration R(g) = 13.6 angstrom, volume v = 16.2 x 10(3) angstrom3 and maximal dimension D(max) = 46 angstrom. The distance distribution function deduced from the scattering measurements is consistent with an overall molecular shape of an oblate ellipsoid of revolution with assymetry parameter nu = 0.45.Departamento de Física, Instituto de Biociências, Letras e Ciências Exatas (IBILCE), Universidade Estadual Paulista (UNESP), São José do Rio Preto, SP, BrasilLaboratório Nacional de Luz Síncroton, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Campinas, SP, BrasilInstituto de Física, Universidade de São Paulo (USP), São Paulo, SP, BrasilDepartamento de Bioquímica, Faculdade de Medicina de Ribeirão Preto (FMRP), Universidade de São Paulo (USP), Ribeirão Preto, SP, BrasilDepartamento de Física, Instituto de Biociências, Letras e Ciências Exatas (IBILCE), Universidade Estadual Paulista (UNESP), São José do Rio Preto, SP, BrasilBiophysical SocietyUniversidade Estadual Paulista (Unesp)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade de São Paulo (USP)Abrego, José Ramon Beltran [UNESP]Craievich, Aldo FelixMascarenhas, Yvonne PrimeranoLaure, Carlos J.2014-05-20T15:22:28Z2014-05-20T15:22:28Z1993-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article560-564application/pdfhttp://dx.doi.org/10.1016/S0006-3495(93)81395-XBiophysical Journal. Bethesda: Biophysical Society, v. 64, n. 2, p. 560-564, 1993.0006-3495http://hdl.handle.net/11449/3343310.1016/S0006-3495(93)81395-XWOS:A1993KN87500030WOSA1993KN87500030.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiophysical Journal3.4951,949info:eu-repo/semantics/openAccess2024-01-21T06:23:48Zoai:repositorio.unesp.br:11449/33433Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:35:32.774069Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv SAXS study of crotapotin at low pH
title SAXS study of crotapotin at low pH
spellingShingle SAXS study of crotapotin at low pH
Abrego, José Ramon Beltran [UNESP]
title_short SAXS study of crotapotin at low pH
title_full SAXS study of crotapotin at low pH
title_fullStr SAXS study of crotapotin at low pH
title_full_unstemmed SAXS study of crotapotin at low pH
title_sort SAXS study of crotapotin at low pH
author Abrego, José Ramon Beltran [UNESP]
author_facet Abrego, José Ramon Beltran [UNESP]
Craievich, Aldo Felix
Mascarenhas, Yvonne Primerano
Laure, Carlos J.
author_role author
author2 Craievich, Aldo Felix
Mascarenhas, Yvonne Primerano
Laure, Carlos J.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Abrego, José Ramon Beltran [UNESP]
Craievich, Aldo Felix
Mascarenhas, Yvonne Primerano
Laure, Carlos J.
description The structure of crotapotin, a protein extracted from the venom of the Crotalus durissus terrificus, in solution at pH = 1.5, was studied by SAXS. The experimental results yield structural parameter values of the molecular radius of gyration R(g) = 13.6 angstrom, volume v = 16.2 x 10(3) angstrom3 and maximal dimension D(max) = 46 angstrom. The distance distribution function deduced from the scattering measurements is consistent with an overall molecular shape of an oblate ellipsoid of revolution with assymetry parameter nu = 0.45.
publishDate 1993
dc.date.none.fl_str_mv 1993-02-01
2014-05-20T15:22:28Z
2014-05-20T15:22:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/S0006-3495(93)81395-X
Biophysical Journal. Bethesda: Biophysical Society, v. 64, n. 2, p. 560-564, 1993.
0006-3495
http://hdl.handle.net/11449/33433
10.1016/S0006-3495(93)81395-X
WOS:A1993KN87500030
WOSA1993KN87500030.pdf
url http://dx.doi.org/10.1016/S0006-3495(93)81395-X
http://hdl.handle.net/11449/33433
identifier_str_mv Biophysical Journal. Bethesda: Biophysical Society, v. 64, n. 2, p. 560-564, 1993.
0006-3495
10.1016/S0006-3495(93)81395-X
WOS:A1993KN87500030
WOSA1993KN87500030.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biophysical Journal
3.495
1,949
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 560-564
application/pdf
dc.publisher.none.fl_str_mv Biophysical Society
publisher.none.fl_str_mv Biophysical Society
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129534553227264

Registros relacionados