Comparative stereologic study between secretory and maturation ameloblasts in rat incisors

Detalhes bibliográficos
Autor(a) principal: Assis, Gerson Francisco De
Data de Publicação: 2003
Outros Autores: Ribeiro, Daniel Araki [UNESP], Campos, Patrícia Danieli, Cestari, Tania Mary, Taga, Rumio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1678-77572003000200011
http://hdl.handle.net/11449/211800
Resumo: The ameloblasts both in secretion phase and in smooth-ended ameloblasts in maturation phase were studied using stereologic methods in transmission electron microscopy (TEM). From secretion to maturation phase of amelogenesis, the nucleus volume decreased 23% and cytoplasm volume did not show significant changes; the total volume and surface of the rough endoplasmic reticulum (RER) decreased 74% and 90%, respectively, and of the mitochondria increased 742% and 384%, respectively; the surface-to-volume ratio for RER and mitochondria decrease 59% and 42%, respectively; and the predominantly apical secretory granules disappeared joined at Tomes’ process and lysosomes and phagic vacuoles have appeared principly in supranuclear cytoplasm. Although significant morphologic variation occurs from the secretory to the maturation phase of the ameloblasts, their cytoplasm volume remains unaltered.
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spelling Comparative stereologic study between secretory and maturation ameloblasts in rat incisorsEstudo estereológico comparativo entre os ameloblastos de secreção e de maturação em incisivos de ratosMaturation and secretory ameloblastsRat incisorsStereologyUltrastructureAmeloblastos de secreção e de maturaçãoIncisivos de ratosEstereologiaUltraestruturaThe ameloblasts both in secretion phase and in smooth-ended ameloblasts in maturation phase were studied using stereologic methods in transmission electron microscopy (TEM). From secretion to maturation phase of amelogenesis, the nucleus volume decreased 23% and cytoplasm volume did not show significant changes; the total volume and surface of the rough endoplasmic reticulum (RER) decreased 74% and 90%, respectively, and of the mitochondria increased 742% and 384%, respectively; the surface-to-volume ratio for RER and mitochondria decrease 59% and 42%, respectively; and the predominantly apical secretory granules disappeared joined at Tomes’ process and lysosomes and phagic vacuoles have appeared principly in supranuclear cytoplasm. Although significant morphologic variation occurs from the secretory to the maturation phase of the ameloblasts, their cytoplasm volume remains unaltered.Ameloblastos são células de origem epitelial, que apresentam funções de síntese, secreção e maturação do esmalte dental. Assim sendo, ameloblastos passam por mudanças morfológicas durante o seu curso de desenvolvimento. Desse modo, cinco ratos adultos Wistar foram utilizados para avaliar a morfologia de ameloblastos da fase secretora e em maturação, usando de métodos estereológicos em microscopia eletrônica de transmissão. Os dados foram analisados pela análise de variância a um critério (ANOVA). Os resultados mostraram que da fase de secreção para a de maturação da amelogênese: a) ocorreu uma redução de 23% no volume absoluto nuclear e o volume absoluto citoplasmático não mostrou diferença estatisticamente significante (p>0.05); b) o volume total e a superfície total do retículo endoplasmático rugoso (RER) diminuíram 74% e 90%, respectivamente; c) o volume total e a superfície total das mitocôndrias aumentaram 742% e 384%, respectivamente; d) a relação superfície-volume do RER e das mitocôndrias diminuíram 59% e 42%, respectivamente; e) os pequenos grânulos de secreção, dispostos principalmente na área apical, desapareceram junto com o Processo de Tomes e apareceram os lisossomos e vacúolos digestivos principalmente na área supranuclear. Portanto, apesar de ocorrer uma significante adaptação morfológica durante a modulação da fase secretora para a de maturação do desenvolvimento do esmalte, o volume citoplasmático do ameloblasto permanece inalterado.Universidade de São Paulo, Faculdade de OdontologiaUniversidade Estadual Paulista, Faculdade de Medicina de BotucatuUniversidade Estadual Paulista, Faculdade de Medicina de BotucatuFaculdade De Odontologia De Bauru - USPUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Assis, Gerson Francisco DeRibeiro, Daniel Araki [UNESP]Campos, Patrícia DanieliCestari, Tania MaryTaga, Rumio2021-07-14T10:29:47Z2021-07-14T10:29:47Z2003-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article144-149application/pdfhttp://dx.doi.org/10.1590/S1678-77572003000200011Journal of Applied Oral Science. Bauru, SP, Brazil: Faculdade De Odontologia De Bauru - USP, v. 11, n. 2, p. 144-149, 2003.1678-77571678-7765http://hdl.handle.net/11449/21180010.1590/S1678-77572003000200011S1678-77572003000200011S1678-77572003000200011.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Oral Scienceinfo:eu-repo/semantics/openAccess2024-01-13T06:36:44Zoai:repositorio.unesp.br:11449/211800Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:53:45.681035Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Comparative stereologic study between secretory and maturation ameloblasts in rat incisors
Estudo estereológico comparativo entre os ameloblastos de secreção e de maturação em incisivos de ratos
title Comparative stereologic study between secretory and maturation ameloblasts in rat incisors
spellingShingle Comparative stereologic study between secretory and maturation ameloblasts in rat incisors
Assis, Gerson Francisco De
Maturation and secretory ameloblasts
Rat incisors
Stereology
Ultrastructure
Ameloblastos de secreção e de maturação
Incisivos de ratos
Estereologia
Ultraestrutura
title_short Comparative stereologic study between secretory and maturation ameloblasts in rat incisors
title_full Comparative stereologic study between secretory and maturation ameloblasts in rat incisors
title_fullStr Comparative stereologic study between secretory and maturation ameloblasts in rat incisors
title_full_unstemmed Comparative stereologic study between secretory and maturation ameloblasts in rat incisors
title_sort Comparative stereologic study between secretory and maturation ameloblasts in rat incisors
author Assis, Gerson Francisco De
author_facet Assis, Gerson Francisco De
Ribeiro, Daniel Araki [UNESP]
Campos, Patrícia Danieli
Cestari, Tania Mary
Taga, Rumio
author_role author
author2 Ribeiro, Daniel Araki [UNESP]
Campos, Patrícia Danieli
Cestari, Tania Mary
Taga, Rumio
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Assis, Gerson Francisco De
Ribeiro, Daniel Araki [UNESP]
Campos, Patrícia Danieli
Cestari, Tania Mary
Taga, Rumio
dc.subject.por.fl_str_mv Maturation and secretory ameloblasts
Rat incisors
Stereology
Ultrastructure
Ameloblastos de secreção e de maturação
Incisivos de ratos
Estereologia
Ultraestrutura
topic Maturation and secretory ameloblasts
Rat incisors
Stereology
Ultrastructure
Ameloblastos de secreção e de maturação
Incisivos de ratos
Estereologia
Ultraestrutura
description The ameloblasts both in secretion phase and in smooth-ended ameloblasts in maturation phase were studied using stereologic methods in transmission electron microscopy (TEM). From secretion to maturation phase of amelogenesis, the nucleus volume decreased 23% and cytoplasm volume did not show significant changes; the total volume and surface of the rough endoplasmic reticulum (RER) decreased 74% and 90%, respectively, and of the mitochondria increased 742% and 384%, respectively; the surface-to-volume ratio for RER and mitochondria decrease 59% and 42%, respectively; and the predominantly apical secretory granules disappeared joined at Tomes’ process and lysosomes and phagic vacuoles have appeared principly in supranuclear cytoplasm. Although significant morphologic variation occurs from the secretory to the maturation phase of the ameloblasts, their cytoplasm volume remains unaltered.
publishDate 2003
dc.date.none.fl_str_mv 2003-06
2021-07-14T10:29:47Z
2021-07-14T10:29:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1678-77572003000200011
Journal of Applied Oral Science. Bauru, SP, Brazil: Faculdade De Odontologia De Bauru - USP, v. 11, n. 2, p. 144-149, 2003.
1678-7757
1678-7765
http://hdl.handle.net/11449/211800
10.1590/S1678-77572003000200011
S1678-77572003000200011
S1678-77572003000200011.pdf
url http://dx.doi.org/10.1590/S1678-77572003000200011
http://hdl.handle.net/11449/211800
identifier_str_mv Journal of Applied Oral Science. Bauru, SP, Brazil: Faculdade De Odontologia De Bauru - USP, v. 11, n. 2, p. 144-149, 2003.
1678-7757
1678-7765
10.1590/S1678-77572003000200011
S1678-77572003000200011
S1678-77572003000200011.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Applied Oral Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 144-149
application/pdf
dc.publisher.none.fl_str_mv Faculdade De Odontologia De Bauru - USP
publisher.none.fl_str_mv Faculdade De Odontologia De Bauru - USP
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129471080824832

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