Morphological correlates of sex differences in acoustic startle response and prepulse inhibition through projections from locus coeruleus to cochlear root neurons
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00429-017-1415-1 http://hdl.handle.net/11449/174440 |
Resumo: | The noradrenergic locus coeruleus (LC) plays an important role in the promotion and maintenance of arousal and alertness. Our group recently described coerulean projections to cochlear root neurons (CRNs), the first relay of the primary acoustic startle reflex (ASR) circuit. However, the role of the LC in the ASR and its modulation, prepulse inhibition (PPI), is not clear. In this study, we damaged LC neurons and fibers using a highly selective neurotoxin, DSP-4, and then assessed ASR and PPI in male and female rats. Our results showed that ASR amplitude was higher in males at 14 days after DSP-4 injection when compared to pre-administration values and those in the male control group. Such modifications in ASR amplitude did not occur in DSP-4-injected females, which exhibited ASR amplitude within the range of control values. PPI differences between males and females seen in controls were not observed in DSP-4-injected rats for any interstimulus interval tested. DSP-4 injection did not affect ASR and PPI latencies in either the male or the female groups, showing values that were consistent with the sex-related variability observed in control rats. Furthermore, we studied the noradrenergic receptor system in the cochlear nerve root using gene expression analysis. When compared to controls, DSP-4-injected males showed higher levels of expression in all adrenoceptor subtypes; however, DSP-4-injected females showed varied effects depending on the receptor type, with either up-, downregulations, or maintenance of expression levels. Lastly, we determined noradrenaline levels in CRNs and other LC-targeted areas using HPLC assays, and these results correlated with behavioral and adrenoceptor expression changes post DSP-4 injection. Our study supports the participation of LC in ASR and PPI, and contributes toward a better understanding of sex-related differences observed in somatosensory gating paradigms. |
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Morphological correlates of sex differences in acoustic startle response and prepulse inhibition through projections from locus coeruleus to cochlear root neuronsAdrenergic receptorsASR pathwayCatecholaminesDopamine beta-hydroxylaseDSP-4NoradrenalineSomatosensory gatingThe noradrenergic locus coeruleus (LC) plays an important role in the promotion and maintenance of arousal and alertness. Our group recently described coerulean projections to cochlear root neurons (CRNs), the first relay of the primary acoustic startle reflex (ASR) circuit. However, the role of the LC in the ASR and its modulation, prepulse inhibition (PPI), is not clear. In this study, we damaged LC neurons and fibers using a highly selective neurotoxin, DSP-4, and then assessed ASR and PPI in male and female rats. Our results showed that ASR amplitude was higher in males at 14 days after DSP-4 injection when compared to pre-administration values and those in the male control group. Such modifications in ASR amplitude did not occur in DSP-4-injected females, which exhibited ASR amplitude within the range of control values. PPI differences between males and females seen in controls were not observed in DSP-4-injected rats for any interstimulus interval tested. DSP-4 injection did not affect ASR and PPI latencies in either the male or the female groups, showing values that were consistent with the sex-related variability observed in control rats. Furthermore, we studied the noradrenergic receptor system in the cochlear nerve root using gene expression analysis. When compared to controls, DSP-4-injected males showed higher levels of expression in all adrenoceptor subtypes; however, DSP-4-injected females showed varied effects depending on the receptor type, with either up-, downregulations, or maintenance of expression levels. Lastly, we determined noradrenaline levels in CRNs and other LC-targeted areas using HPLC assays, and these results correlated with behavioral and adrenoceptor expression changes post DSP-4 injection. Our study supports the participation of LC in ASR and PPI, and contributes toward a better understanding of sex-related differences observed in somatosensory gating paradigms.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Ministerio de Ciencia e InnovaciónMinisterio de Economía y CompetitividadInstitute for Neuroscience of Castilla y León (INCYL) University of SalamancaInstitute of Biomedical Research of Salamanca (IBSAL) University of SalamancaDepartment of Cell Biology and Pathology University of SalamancaDepartment of Physiology and Pharmacology University of SalamancaDepartment of Anatomy Institute of Biosciences of Botucatu Univ. Estadual Paulista (UNESP), Distrito de Rubião Jr., S/N, PO.Box 510Department of Anatomy Institute of Biosciences of Botucatu Univ. Estadual Paulista (UNESP), Distrito de Rubião Jr., S/N, PO.Box 510FAPESP: 2008/02771-6Ministerio de Ciencia e Innovación: BFU2010-17754Ministerio de Economía y Competitividad: SAF2016-78898-C2-2RUniversity of SalamancaUniversidade Estadual Paulista (Unesp)Hormigo, SebastianGómez-Nieto, RicardoSancho, ConsueloHerrero-Turrión, JavierCarro, JuanLópez, Dolores E.Horta-Júnior, Joséde Anchietade Castroe [UNESP]2018-12-11T17:11:08Z2018-12-11T17:11:08Z2017-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3491-3508application/pdfhttp://dx.doi.org/10.1007/s00429-017-1415-1Brain Structure and Function, v. 222, n. 8, p. 3491-3508, 2017.1863-26611863-2653http://hdl.handle.net/11449/17444010.1007/s00429-017-1415-12-s2.0-850171821082-s2.0-85017182108.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrain Structure and Function2,0342,034info:eu-repo/semantics/openAccess2023-10-15T06:10:46Zoai:repositorio.unesp.br:11449/174440Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:02:28.625153Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Morphological correlates of sex differences in acoustic startle response and prepulse inhibition through projections from locus coeruleus to cochlear root neurons |
title |
Morphological correlates of sex differences in acoustic startle response and prepulse inhibition through projections from locus coeruleus to cochlear root neurons |
spellingShingle |
Morphological correlates of sex differences in acoustic startle response and prepulse inhibition through projections from locus coeruleus to cochlear root neurons Hormigo, Sebastian Adrenergic receptors ASR pathway Catecholamines Dopamine beta-hydroxylase DSP-4 Noradrenaline Somatosensory gating |
title_short |
Morphological correlates of sex differences in acoustic startle response and prepulse inhibition through projections from locus coeruleus to cochlear root neurons |
title_full |
Morphological correlates of sex differences in acoustic startle response and prepulse inhibition through projections from locus coeruleus to cochlear root neurons |
title_fullStr |
Morphological correlates of sex differences in acoustic startle response and prepulse inhibition through projections from locus coeruleus to cochlear root neurons |
title_full_unstemmed |
Morphological correlates of sex differences in acoustic startle response and prepulse inhibition through projections from locus coeruleus to cochlear root neurons |
title_sort |
Morphological correlates of sex differences in acoustic startle response and prepulse inhibition through projections from locus coeruleus to cochlear root neurons |
author |
Hormigo, Sebastian |
author_facet |
Hormigo, Sebastian Gómez-Nieto, Ricardo Sancho, Consuelo Herrero-Turrión, Javier Carro, Juan López, Dolores E. Horta-Júnior, Joséde Anchietade Castroe [UNESP] |
author_role |
author |
author2 |
Gómez-Nieto, Ricardo Sancho, Consuelo Herrero-Turrión, Javier Carro, Juan López, Dolores E. Horta-Júnior, Joséde Anchietade Castroe [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
University of Salamanca Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Hormigo, Sebastian Gómez-Nieto, Ricardo Sancho, Consuelo Herrero-Turrión, Javier Carro, Juan López, Dolores E. Horta-Júnior, Joséde Anchietade Castroe [UNESP] |
dc.subject.por.fl_str_mv |
Adrenergic receptors ASR pathway Catecholamines Dopamine beta-hydroxylase DSP-4 Noradrenaline Somatosensory gating |
topic |
Adrenergic receptors ASR pathway Catecholamines Dopamine beta-hydroxylase DSP-4 Noradrenaline Somatosensory gating |
description |
The noradrenergic locus coeruleus (LC) plays an important role in the promotion and maintenance of arousal and alertness. Our group recently described coerulean projections to cochlear root neurons (CRNs), the first relay of the primary acoustic startle reflex (ASR) circuit. However, the role of the LC in the ASR and its modulation, prepulse inhibition (PPI), is not clear. In this study, we damaged LC neurons and fibers using a highly selective neurotoxin, DSP-4, and then assessed ASR and PPI in male and female rats. Our results showed that ASR amplitude was higher in males at 14 days after DSP-4 injection when compared to pre-administration values and those in the male control group. Such modifications in ASR amplitude did not occur in DSP-4-injected females, which exhibited ASR amplitude within the range of control values. PPI differences between males and females seen in controls were not observed in DSP-4-injected rats for any interstimulus interval tested. DSP-4 injection did not affect ASR and PPI latencies in either the male or the female groups, showing values that were consistent with the sex-related variability observed in control rats. Furthermore, we studied the noradrenergic receptor system in the cochlear nerve root using gene expression analysis. When compared to controls, DSP-4-injected males showed higher levels of expression in all adrenoceptor subtypes; however, DSP-4-injected females showed varied effects depending on the receptor type, with either up-, downregulations, or maintenance of expression levels. Lastly, we determined noradrenaline levels in CRNs and other LC-targeted areas using HPLC assays, and these results correlated with behavioral and adrenoceptor expression changes post DSP-4 injection. Our study supports the participation of LC in ASR and PPI, and contributes toward a better understanding of sex-related differences observed in somatosensory gating paradigms. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-01 2018-12-11T17:11:08Z 2018-12-11T17:11:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00429-017-1415-1 Brain Structure and Function, v. 222, n. 8, p. 3491-3508, 2017. 1863-2661 1863-2653 http://hdl.handle.net/11449/174440 10.1007/s00429-017-1415-1 2-s2.0-85017182108 2-s2.0-85017182108.pdf |
url |
http://dx.doi.org/10.1007/s00429-017-1415-1 http://hdl.handle.net/11449/174440 |
identifier_str_mv |
Brain Structure and Function, v. 222, n. 8, p. 3491-3508, 2017. 1863-2661 1863-2653 10.1007/s00429-017-1415-1 2-s2.0-85017182108 2-s2.0-85017182108.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brain Structure and Function 2,034 2,034 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3491-3508 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128451293478912 |