Diversity of haemogregarine parasites infecting Brazilian snakes from the Midwest and Southeast regions with a description of two new species of Hepatozoon (Apicomplexa: Adeleorina: Hepatozoidae)

Detalhes bibliográficos
Autor(a) principal: Úngari, Letícia Pereira [UNESP]
Data de Publicação: 2022
Outros Autores: Netherlands, Edward C., Santos, André Luiz Quagliatto, Alcantara, Edna Paulino de [UNESP], Emmerich, Enzo [UNESP], Silva, Reinaldo José da [UNESP], O'Dwyer, Lucia Helena [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.parint.2022.102587
http://hdl.handle.net/11449/239980
Resumo: Although Brazil is a hotspot for snake species, there is a lack of information on the biodiversity of haemoparasites infecting these hosts. Thus, the present study aimed to bring new insights on the diversity of species of Hepatozoon (Apicomplexa: Adeleorina: Hepatozoidae) infecting Brazilian snakes from the Midwest and Southeast regions. The snakes were captured from 2018 to 2020 from the states of Mato Grosso, Mato Grosso do Sul, Goiás, and São Paulo. Three to five blood smears were made and the remaining blood sample was stored for further molecular analysis. Moreover, histopathological slides of the organs were stained with haematoxylin-eosin. Regarding molecular diagnosis, PCR was performed targeting different regions of the 18S rRNA gene of apicomplexan parasites. From the 13 free-living snakes screened, ten (76.92%) were found infected with Hepatozoon spp. Based on morphological and morphometric tools, five different morphotypes of species of Hepatozoon gamonts were detected. Molecular data and phylogenetic analysis support the morphological data, identifying five species of Hepatozoon from snakes, of which three species belong to previously described species, Hepatozoon cevapii, Hepatozoon cuestensis, and Hepatozoon quagliattus, with a genetic similarity of 100% (based on the 18S rRNA genetic marker). The present study identifies and describes two new species of Hepatozoon, Hepatozoon annulatum sp. nov. infecting the snake Leptodeira annulata and Hepatozoon trigeminum sp. nov. infecting the snake Oxyrhopus trigeminus. Thus, based on morphological and molecular data the present study provides new insights on haemogregarine diversity infecting Brazilian snakes from the Midwest and Southeast regions.
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spelling Diversity of haemogregarine parasites infecting Brazilian snakes from the Midwest and Southeast regions with a description of two new species of Hepatozoon (Apicomplexa: Adeleorina: Hepatozoidae)18S rRNABiodiversityHaemogregarineSnakeAlthough Brazil is a hotspot for snake species, there is a lack of information on the biodiversity of haemoparasites infecting these hosts. Thus, the present study aimed to bring new insights on the diversity of species of Hepatozoon (Apicomplexa: Adeleorina: Hepatozoidae) infecting Brazilian snakes from the Midwest and Southeast regions. The snakes were captured from 2018 to 2020 from the states of Mato Grosso, Mato Grosso do Sul, Goiás, and São Paulo. Three to five blood smears were made and the remaining blood sample was stored for further molecular analysis. Moreover, histopathological slides of the organs were stained with haematoxylin-eosin. Regarding molecular diagnosis, PCR was performed targeting different regions of the 18S rRNA gene of apicomplexan parasites. From the 13 free-living snakes screened, ten (76.92%) were found infected with Hepatozoon spp. Based on morphological and morphometric tools, five different morphotypes of species of Hepatozoon gamonts were detected. Molecular data and phylogenetic analysis support the morphological data, identifying five species of Hepatozoon from snakes, of which three species belong to previously described species, Hepatozoon cevapii, Hepatozoon cuestensis, and Hepatozoon quagliattus, with a genetic similarity of 100% (based on the 18S rRNA genetic marker). The present study identifies and describes two new species of Hepatozoon, Hepatozoon annulatum sp. nov. infecting the snake Leptodeira annulata and Hepatozoon trigeminum sp. nov. infecting the snake Oxyrhopus trigeminus. Thus, based on morphological and molecular data the present study provides new insights on haemogregarine diversity infecting Brazilian snakes from the Midwest and Southeast regions.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Instituto de Biociências Universidade Estadual Paulista UNESP DBBVPZ Setor de Parasitologia, Distrito de Ribeirão Junior, São PauloDepartment Zoology and Entomology University of the Free State, PO Box 339Laboratório de Ensino e Pesquisa em Animais Silvestres Faculdade de Medicina Veterinária Universidade Federal de UberlândiaInstituto de Biociências Universidade Estadual Paulista UNESP DBBVPZ Setor de Parasitologia, Distrito de Ribeirão Junior, São PauloFAPESP: 2016/50377-1FAPESP: 2018/00754-9FAPESP: 2018/09623-4CNPq: 309125/2017-0Universidade Estadual Paulista (UNESP)University of the Free StateUniversidade Federal de Uberlândia (UFU)Úngari, Letícia Pereira [UNESP]Netherlands, Edward C.Santos, André Luiz QuagliattoAlcantara, Edna Paulino de [UNESP]Emmerich, Enzo [UNESP]Silva, Reinaldo José da [UNESP]O'Dwyer, Lucia Helena [UNESP]2023-03-01T19:56:12Z2023-03-01T19:56:12Z2022-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.parint.2022.102587Parasitology International, v. 89.1873-03291383-5769http://hdl.handle.net/11449/23998010.1016/j.parint.2022.1025872-s2.0-85129308859Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengParasitology Internationalinfo:eu-repo/semantics/openAccess2023-03-01T19:56:13Zoai:repositorio.unesp.br:11449/239980Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:29:46.541060Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Diversity of haemogregarine parasites infecting Brazilian snakes from the Midwest and Southeast regions with a description of two new species of Hepatozoon (Apicomplexa: Adeleorina: Hepatozoidae)
title Diversity of haemogregarine parasites infecting Brazilian snakes from the Midwest and Southeast regions with a description of two new species of Hepatozoon (Apicomplexa: Adeleorina: Hepatozoidae)
spellingShingle Diversity of haemogregarine parasites infecting Brazilian snakes from the Midwest and Southeast regions with a description of two new species of Hepatozoon (Apicomplexa: Adeleorina: Hepatozoidae)
Úngari, Letícia Pereira [UNESP]
18S rRNA
Biodiversity
Haemogregarine
Snake
title_short Diversity of haemogregarine parasites infecting Brazilian snakes from the Midwest and Southeast regions with a description of two new species of Hepatozoon (Apicomplexa: Adeleorina: Hepatozoidae)
title_full Diversity of haemogregarine parasites infecting Brazilian snakes from the Midwest and Southeast regions with a description of two new species of Hepatozoon (Apicomplexa: Adeleorina: Hepatozoidae)
title_fullStr Diversity of haemogregarine parasites infecting Brazilian snakes from the Midwest and Southeast regions with a description of two new species of Hepatozoon (Apicomplexa: Adeleorina: Hepatozoidae)
title_full_unstemmed Diversity of haemogregarine parasites infecting Brazilian snakes from the Midwest and Southeast regions with a description of two new species of Hepatozoon (Apicomplexa: Adeleorina: Hepatozoidae)
title_sort Diversity of haemogregarine parasites infecting Brazilian snakes from the Midwest and Southeast regions with a description of two new species of Hepatozoon (Apicomplexa: Adeleorina: Hepatozoidae)
author Úngari, Letícia Pereira [UNESP]
author_facet Úngari, Letícia Pereira [UNESP]
Netherlands, Edward C.
Santos, André Luiz Quagliatto
Alcantara, Edna Paulino de [UNESP]
Emmerich, Enzo [UNESP]
Silva, Reinaldo José da [UNESP]
O'Dwyer, Lucia Helena [UNESP]
author_role author
author2 Netherlands, Edward C.
Santos, André Luiz Quagliatto
Alcantara, Edna Paulino de [UNESP]
Emmerich, Enzo [UNESP]
Silva, Reinaldo José da [UNESP]
O'Dwyer, Lucia Helena [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
University of the Free State
Universidade Federal de Uberlândia (UFU)
dc.contributor.author.fl_str_mv Úngari, Letícia Pereira [UNESP]
Netherlands, Edward C.
Santos, André Luiz Quagliatto
Alcantara, Edna Paulino de [UNESP]
Emmerich, Enzo [UNESP]
Silva, Reinaldo José da [UNESP]
O'Dwyer, Lucia Helena [UNESP]
dc.subject.por.fl_str_mv 18S rRNA
Biodiversity
Haemogregarine
Snake
topic 18S rRNA
Biodiversity
Haemogregarine
Snake
description Although Brazil is a hotspot for snake species, there is a lack of information on the biodiversity of haemoparasites infecting these hosts. Thus, the present study aimed to bring new insights on the diversity of species of Hepatozoon (Apicomplexa: Adeleorina: Hepatozoidae) infecting Brazilian snakes from the Midwest and Southeast regions. The snakes were captured from 2018 to 2020 from the states of Mato Grosso, Mato Grosso do Sul, Goiás, and São Paulo. Three to five blood smears were made and the remaining blood sample was stored for further molecular analysis. Moreover, histopathological slides of the organs were stained with haematoxylin-eosin. Regarding molecular diagnosis, PCR was performed targeting different regions of the 18S rRNA gene of apicomplexan parasites. From the 13 free-living snakes screened, ten (76.92%) were found infected with Hepatozoon spp. Based on morphological and morphometric tools, five different morphotypes of species of Hepatozoon gamonts were detected. Molecular data and phylogenetic analysis support the morphological data, identifying five species of Hepatozoon from snakes, of which three species belong to previously described species, Hepatozoon cevapii, Hepatozoon cuestensis, and Hepatozoon quagliattus, with a genetic similarity of 100% (based on the 18S rRNA genetic marker). The present study identifies and describes two new species of Hepatozoon, Hepatozoon annulatum sp. nov. infecting the snake Leptodeira annulata and Hepatozoon trigeminum sp. nov. infecting the snake Oxyrhopus trigeminus. Thus, based on morphological and molecular data the present study provides new insights on haemogregarine diversity infecting Brazilian snakes from the Midwest and Southeast regions.
publishDate 2022
dc.date.none.fl_str_mv 2022-08-01
2023-03-01T19:56:12Z
2023-03-01T19:56:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.parint.2022.102587
Parasitology International, v. 89.
1873-0329
1383-5769
http://hdl.handle.net/11449/239980
10.1016/j.parint.2022.102587
2-s2.0-85129308859
url http://dx.doi.org/10.1016/j.parint.2022.102587
http://hdl.handle.net/11449/239980
identifier_str_mv Parasitology International, v. 89.
1873-0329
1383-5769
10.1016/j.parint.2022.102587
2-s2.0-85129308859
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Parasitology International
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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