Perinatal exposure to bisphenol A impacts in the mammary gland morphology of adult Mongolian gerbils
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.yexmp.2020.104374 http://hdl.handle.net/11449/201467 |
Resumo: | The endocrine disruptive effects caused by bisphenol A (BPA) are well known. Despite this, to date, evaluation of its long term effects is limited, meaning that there is still much to be unveiled in terms of alterations caused by perinatal exposure to BPA. Our aim was to determine if perinatal exposure to two different doses of BPA causes long term morphological and molecular alteration effects in the mammary gland (MG). We evaluated MG from Mongolian gerbil offspring exposed perinatally (during gestation and lactation) to 50 or 5000 μg/kg/day BPA. At 90 days of age the animals were subjected to a single dose of N-nitroso-N-methylurea in order to mimic a carcinogenic environment. At 6 months of age, animals in estrous were euthanized for morphological evaluation of the MGs. The MG architecture presented considerable changes in terms of detached epithelial cells, inflammation, glandular hyperplasia, and collagen fiber deposition. Furthermore, a higher index of epithelial cell proliferation was detected in comparison to the intact control group. In addition, we verified a higher molecular expression of EZH2 in the vehicle treated group, indicating that corn oil applied alone can alter the expression of this epigenetic biomarker. In conclusion, BPA perinatal exposure promotes significant changes in glandular cytoarchitecture and increases glandular epithelium proliferation rate, leading to the retention of stem-like properties. This event could compromise the fate and differentiation potential of mammary epithelium. |
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Perinatal exposure to bisphenol A impacts in the mammary gland morphology of adult Mongolian gerbilsBPAEnvironment pollutantEstrogenEZH2Morphologic alterationsPhospho-histone-h3The endocrine disruptive effects caused by bisphenol A (BPA) are well known. Despite this, to date, evaluation of its long term effects is limited, meaning that there is still much to be unveiled in terms of alterations caused by perinatal exposure to BPA. Our aim was to determine if perinatal exposure to two different doses of BPA causes long term morphological and molecular alteration effects in the mammary gland (MG). We evaluated MG from Mongolian gerbil offspring exposed perinatally (during gestation and lactation) to 50 or 5000 μg/kg/day BPA. At 90 days of age the animals were subjected to a single dose of N-nitroso-N-methylurea in order to mimic a carcinogenic environment. At 6 months of age, animals in estrous were euthanized for morphological evaluation of the MGs. The MG architecture presented considerable changes in terms of detached epithelial cells, inflammation, glandular hyperplasia, and collagen fiber deposition. Furthermore, a higher index of epithelial cell proliferation was detected in comparison to the intact control group. In addition, we verified a higher molecular expression of EZH2 in the vehicle treated group, indicating that corn oil applied alone can alter the expression of this epigenetic biomarker. In conclusion, BPA perinatal exposure promotes significant changes in glandular cytoarchitecture and increases glandular epithelium proliferation rate, leading to the retention of stem-like properties. This event could compromise the fate and differentiation potential of mammary epithelium.Department of Biology Institute of Biosciences Humanities and Exact Sciences São Paulo State University (UNESP), Rua Cristóvão Colombo 2265, Jardim NazarethFederal University of São João del Rei, Campus Centro Oeste Dona Lindu, Avenida Sebastião Gonçalves Coelho, 400, Bairro ChanadourLaboratory of Gynecology Institute of Experimental and Clinique Research Université Catholique de Louvain (UCL), Avenue Mounier 52, bte B1.52.02Department of Biology Institute of Biosciences Humanities and Exact Sciences São Paulo State University (UNESP), Rua Cristóvão Colombo 2265, Jardim NazarethUniversidade Estadual Paulista (Unesp)Federal University of São João del ReiUniversité Catholique de Louvain (UCL)Leonel, Ellen Cristina Rivas [UNESP]Campos, Silvana Gisele PegorinBedolo, Carolina Marques Baraldi [UNESP]Guerra, Luiz Henrique Alves [UNESP]Vilamaior, Patrícia Simone Leite [UNESP]Calmon, Marilia Freitas [UNESP]Rahal, Paula [UNESP]Amorim, Christiani AndradeTaboga, Sebastião Roberto [UNESP]2020-12-12T02:33:14Z2020-12-12T02:33:14Z2020-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.yexmp.2020.104374Experimental and Molecular Pathology, v. 113.1096-09450014-4800http://hdl.handle.net/11449/20146710.1016/j.yexmp.2020.1043742-s2.0-85077756192Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengExperimental and Molecular Pathologyinfo:eu-repo/semantics/openAccess2021-10-22T19:50:44Zoai:repositorio.unesp.br:11449/201467Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:29:28.786049Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Perinatal exposure to bisphenol A impacts in the mammary gland morphology of adult Mongolian gerbils |
title |
Perinatal exposure to bisphenol A impacts in the mammary gland morphology of adult Mongolian gerbils |
spellingShingle |
Perinatal exposure to bisphenol A impacts in the mammary gland morphology of adult Mongolian gerbils Leonel, Ellen Cristina Rivas [UNESP] BPA Environment pollutant Estrogen EZH2 Morphologic alterations Phospho-histone-h3 |
title_short |
Perinatal exposure to bisphenol A impacts in the mammary gland morphology of adult Mongolian gerbils |
title_full |
Perinatal exposure to bisphenol A impacts in the mammary gland morphology of adult Mongolian gerbils |
title_fullStr |
Perinatal exposure to bisphenol A impacts in the mammary gland morphology of adult Mongolian gerbils |
title_full_unstemmed |
Perinatal exposure to bisphenol A impacts in the mammary gland morphology of adult Mongolian gerbils |
title_sort |
Perinatal exposure to bisphenol A impacts in the mammary gland morphology of adult Mongolian gerbils |
author |
Leonel, Ellen Cristina Rivas [UNESP] |
author_facet |
Leonel, Ellen Cristina Rivas [UNESP] Campos, Silvana Gisele Pegorin Bedolo, Carolina Marques Baraldi [UNESP] Guerra, Luiz Henrique Alves [UNESP] Vilamaior, Patrícia Simone Leite [UNESP] Calmon, Marilia Freitas [UNESP] Rahal, Paula [UNESP] Amorim, Christiani Andrade Taboga, Sebastião Roberto [UNESP] |
author_role |
author |
author2 |
Campos, Silvana Gisele Pegorin Bedolo, Carolina Marques Baraldi [UNESP] Guerra, Luiz Henrique Alves [UNESP] Vilamaior, Patrícia Simone Leite [UNESP] Calmon, Marilia Freitas [UNESP] Rahal, Paula [UNESP] Amorim, Christiani Andrade Taboga, Sebastião Roberto [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Federal University of São João del Rei Université Catholique de Louvain (UCL) |
dc.contributor.author.fl_str_mv |
Leonel, Ellen Cristina Rivas [UNESP] Campos, Silvana Gisele Pegorin Bedolo, Carolina Marques Baraldi [UNESP] Guerra, Luiz Henrique Alves [UNESP] Vilamaior, Patrícia Simone Leite [UNESP] Calmon, Marilia Freitas [UNESP] Rahal, Paula [UNESP] Amorim, Christiani Andrade Taboga, Sebastião Roberto [UNESP] |
dc.subject.por.fl_str_mv |
BPA Environment pollutant Estrogen EZH2 Morphologic alterations Phospho-histone-h3 |
topic |
BPA Environment pollutant Estrogen EZH2 Morphologic alterations Phospho-histone-h3 |
description |
The endocrine disruptive effects caused by bisphenol A (BPA) are well known. Despite this, to date, evaluation of its long term effects is limited, meaning that there is still much to be unveiled in terms of alterations caused by perinatal exposure to BPA. Our aim was to determine if perinatal exposure to two different doses of BPA causes long term morphological and molecular alteration effects in the mammary gland (MG). We evaluated MG from Mongolian gerbil offspring exposed perinatally (during gestation and lactation) to 50 or 5000 μg/kg/day BPA. At 90 days of age the animals were subjected to a single dose of N-nitroso-N-methylurea in order to mimic a carcinogenic environment. At 6 months of age, animals in estrous were euthanized for morphological evaluation of the MGs. The MG architecture presented considerable changes in terms of detached epithelial cells, inflammation, glandular hyperplasia, and collagen fiber deposition. Furthermore, a higher index of epithelial cell proliferation was detected in comparison to the intact control group. In addition, we verified a higher molecular expression of EZH2 in the vehicle treated group, indicating that corn oil applied alone can alter the expression of this epigenetic biomarker. In conclusion, BPA perinatal exposure promotes significant changes in glandular cytoarchitecture and increases glandular epithelium proliferation rate, leading to the retention of stem-like properties. This event could compromise the fate and differentiation potential of mammary epithelium. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:33:14Z 2020-12-12T02:33:14Z 2020-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.yexmp.2020.104374 Experimental and Molecular Pathology, v. 113. 1096-0945 0014-4800 http://hdl.handle.net/11449/201467 10.1016/j.yexmp.2020.104374 2-s2.0-85077756192 |
url |
http://dx.doi.org/10.1016/j.yexmp.2020.104374 http://hdl.handle.net/11449/201467 |
identifier_str_mv |
Experimental and Molecular Pathology, v. 113. 1096-0945 0014-4800 10.1016/j.yexmp.2020.104374 2-s2.0-85077756192 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Experimental and Molecular Pathology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129325662208000 |