Novel viruses of the family Partitiviridae discovered in Saccharomyces cerevisiae
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.ppat.1011418 http://hdl.handle.net/11449/245662 |
Resumo: | It has been 49 years since the last discovery of a new virus family in the model yeast Saccharomyces cerevisiae. A large-scale screen to determine the diversity of double-stranded RNA (dsRNA) viruses in S. cerevisiae has identified multiple novel viruses from the family Partitiviridae that have been previously shown to infect plants, fungi, protozoans, and insects. Most S. cerevisiae partitiviruses (ScPVs) are associated with strains of yeasts isolated from coffee and cacao beans. The presence of partitiviruses was confirmed by sequencing the viral dsRNAs and purifying and visualizing isometric, non-enveloped viral particles. ScPVs have a typical bipartite genome encoding an RNA-dependent RNA polymerase (RdRP) and a coat protein (CP). Phylogenetic analysis of ScPVs identified three species of ScPV, which are most closely related to viruses of the genus Cryspovirus from the mammalian pathogenic protozoan Cryptosporidium parvum. Molecular modeling of the ScPV RdRP revealed a conserved tertiary structure and catalytic site organization when compared to the RdRPs of the Picornaviridae. The ScPV CP is the smallest so far identified in the Partitiviridae and has structural homology with the CP of other partitiviruses but likely lacks a protrusion domain that is a conspicuous feature of other partitivirus particles. ScPVs were stably maintained during laboratory growth and were successfully transferred to haploid progeny after sporulation, which provides future opportunities to study partitivirus-host interactions using the powerful genetic tools available for the model organism S. cerevisiae. Author summaryThis article describes the discovery and characterization of multiple strains and species of viruses from the family Partitiviridae in the brewer's and baker's yeast S. cerevisiae. These novel viruses have bipartite genomes packaged in spherical viral particles with structural homology to members of the family Partitiviridae. Strikingly, yeast partitiviruses are most closely related to viruses from human pathogenic protozoa and not partitiviruses of other fungi. As partitiviruses can positively and negatively contribute to a host's physiology (including important human and plant pathogens), the presence of partitiviruses in S. cerevisiae offers a unique opportunity to study the biology of these viruses in a well-developed model system. |
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Novel viruses of the family Partitiviridae discovered in Saccharomyces cerevisiaeIt has been 49 years since the last discovery of a new virus family in the model yeast Saccharomyces cerevisiae. A large-scale screen to determine the diversity of double-stranded RNA (dsRNA) viruses in S. cerevisiae has identified multiple novel viruses from the family Partitiviridae that have been previously shown to infect plants, fungi, protozoans, and insects. Most S. cerevisiae partitiviruses (ScPVs) are associated with strains of yeasts isolated from coffee and cacao beans. The presence of partitiviruses was confirmed by sequencing the viral dsRNAs and purifying and visualizing isometric, non-enveloped viral particles. ScPVs have a typical bipartite genome encoding an RNA-dependent RNA polymerase (RdRP) and a coat protein (CP). Phylogenetic analysis of ScPVs identified three species of ScPV, which are most closely related to viruses of the genus Cryspovirus from the mammalian pathogenic protozoan Cryptosporidium parvum. Molecular modeling of the ScPV RdRP revealed a conserved tertiary structure and catalytic site organization when compared to the RdRPs of the Picornaviridae. The ScPV CP is the smallest so far identified in the Partitiviridae and has structural homology with the CP of other partitiviruses but likely lacks a protrusion domain that is a conspicuous feature of other partitivirus particles. ScPVs were stably maintained during laboratory growth and were successfully transferred to haploid progeny after sporulation, which provides future opportunities to study partitivirus-host interactions using the powerful genetic tools available for the model organism S. cerevisiae. Author summaryThis article describes the discovery and characterization of multiple strains and species of viruses from the family Partitiviridae in the brewer's and baker's yeast S. cerevisiae. These novel viruses have bipartite genomes packaged in spherical viral particles with structural homology to members of the family Partitiviridae. Strikingly, yeast partitiviruses are most closely related to viruses from human pathogenic protozoa and not partitiviruses of other fungi. As partitiviruses can positively and negatively contribute to a host's physiology (including important human and plant pathogens), the presence of partitiviruses in S. cerevisiae offers a unique opportunity to study the biology of these viruses in a well-developed model system.National Institute of General Medical Sciences of the National Institutes of HealthNational Science Foundation Division of Molecular and Cellular BiosciencesNational Science Foundation EPSCoR Research Infrastructure Improvement Program: Track-2National Institutes of HealthOffice of Nuclear Energy of the U.S. DOENuclear Science User FacilitiesFunda��o de Amparo � Pesquisa do Estado de S�o Paulo (FAPESP)University of Idaho Office of Undergraduate ResearchDepartment of Biological SciencesDyess Faculty Fellowship, University of Idaho College of ScienceUniv Idaho, Dept Biol Sci, Moscow, ID 83844 USASao Paulo State Univ UNESP, Dept Gen & Appl Biol, Rio Claro, SP, BrazilSao Paulo State Univ UNESP, Ctr Study Social Insects, Rio Claro, SP, BrazilPortland State Univ, Ctr Life Extreme Environm, Dept Biol, Portland, OR USAUniv Idaho, Inst Modeling Collaborat & Innovat, Moscow, ID USAUniv Idaho, Dept Phys, Moscow, ID USASao Paulo State Univ UNESP, Dept Gen & Appl Biol, Rio Claro, SP, BrazilSao Paulo State Univ UNESP, Ctr Study Social Insects, Rio Claro, SP, BrazilNational Institute of General Medical Sciences of the National Institutes of Health: P20 GM104420National Science Foundation Division of Molecular and Cellular Biosciences: 1818368National Science Foundation Division of Molecular and Cellular Biosciences: 2025305National Science Foundation EPSCoR Research Infrastructure Improvement Program: Track-2: OIA-1736253National Institutes of Health: P30 GM103324Nuclear Science User Facilities: DE-AC07-05ID14517FAPESP: 2021/09980-4Public Library ScienceUniv IdahoUniversidade Estadual Paulista (UNESP)Portland State UnivTaggart, Nathan T. E.Crabtree, Angela M. A.Creagh, Jack W. M.Bizarria, Rodolfo M. [UNESP]Li, Shunjila Higuera, Ignacio A. deBarnes, Jonathan E.Shipley, Mason A.Boyer, Josephine M.Stedman, Kenneth M.Ytreberg, F. MartyRowley, Paul A.2023-07-29T12:01:23Z2023-07-29T12:01:23Z2023-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article22http://dx.doi.org/10.1371/journal.ppat.1011418Plos Pathogens. San Francisco: Public Library Science, v. 19, n. 6, 22 p., 2023.1553-7366http://hdl.handle.net/11449/24566210.1371/journal.ppat.1011418WOS:001003237700002Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos Pathogensinfo:eu-repo/semantics/openAccess2023-07-29T12:01:23Zoai:repositorio.unesp.br:11449/245662Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:21:05.103642Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Novel viruses of the family Partitiviridae discovered in Saccharomyces cerevisiae |
title |
Novel viruses of the family Partitiviridae discovered in Saccharomyces cerevisiae |
spellingShingle |
Novel viruses of the family Partitiviridae discovered in Saccharomyces cerevisiae Taggart, Nathan T. E. |
title_short |
Novel viruses of the family Partitiviridae discovered in Saccharomyces cerevisiae |
title_full |
Novel viruses of the family Partitiviridae discovered in Saccharomyces cerevisiae |
title_fullStr |
Novel viruses of the family Partitiviridae discovered in Saccharomyces cerevisiae |
title_full_unstemmed |
Novel viruses of the family Partitiviridae discovered in Saccharomyces cerevisiae |
title_sort |
Novel viruses of the family Partitiviridae discovered in Saccharomyces cerevisiae |
author |
Taggart, Nathan T. E. |
author_facet |
Taggart, Nathan T. E. Crabtree, Angela M. A. Creagh, Jack W. M. Bizarria, Rodolfo M. [UNESP] Li, Shunji la Higuera, Ignacio A. de Barnes, Jonathan E. Shipley, Mason A. Boyer, Josephine M. Stedman, Kenneth M. Ytreberg, F. Marty Rowley, Paul A. |
author_role |
author |
author2 |
Crabtree, Angela M. A. Creagh, Jack W. M. Bizarria, Rodolfo M. [UNESP] Li, Shunji la Higuera, Ignacio A. de Barnes, Jonathan E. Shipley, Mason A. Boyer, Josephine M. Stedman, Kenneth M. Ytreberg, F. Marty Rowley, Paul A. |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Idaho Universidade Estadual Paulista (UNESP) Portland State Univ |
dc.contributor.author.fl_str_mv |
Taggart, Nathan T. E. Crabtree, Angela M. A. Creagh, Jack W. M. Bizarria, Rodolfo M. [UNESP] Li, Shunji la Higuera, Ignacio A. de Barnes, Jonathan E. Shipley, Mason A. Boyer, Josephine M. Stedman, Kenneth M. Ytreberg, F. Marty Rowley, Paul A. |
description |
It has been 49 years since the last discovery of a new virus family in the model yeast Saccharomyces cerevisiae. A large-scale screen to determine the diversity of double-stranded RNA (dsRNA) viruses in S. cerevisiae has identified multiple novel viruses from the family Partitiviridae that have been previously shown to infect plants, fungi, protozoans, and insects. Most S. cerevisiae partitiviruses (ScPVs) are associated with strains of yeasts isolated from coffee and cacao beans. The presence of partitiviruses was confirmed by sequencing the viral dsRNAs and purifying and visualizing isometric, non-enveloped viral particles. ScPVs have a typical bipartite genome encoding an RNA-dependent RNA polymerase (RdRP) and a coat protein (CP). Phylogenetic analysis of ScPVs identified three species of ScPV, which are most closely related to viruses of the genus Cryspovirus from the mammalian pathogenic protozoan Cryptosporidium parvum. Molecular modeling of the ScPV RdRP revealed a conserved tertiary structure and catalytic site organization when compared to the RdRPs of the Picornaviridae. The ScPV CP is the smallest so far identified in the Partitiviridae and has structural homology with the CP of other partitiviruses but likely lacks a protrusion domain that is a conspicuous feature of other partitivirus particles. ScPVs were stably maintained during laboratory growth and were successfully transferred to haploid progeny after sporulation, which provides future opportunities to study partitivirus-host interactions using the powerful genetic tools available for the model organism S. cerevisiae. Author summaryThis article describes the discovery and characterization of multiple strains and species of viruses from the family Partitiviridae in the brewer's and baker's yeast S. cerevisiae. These novel viruses have bipartite genomes packaged in spherical viral particles with structural homology to members of the family Partitiviridae. Strikingly, yeast partitiviruses are most closely related to viruses from human pathogenic protozoa and not partitiviruses of other fungi. As partitiviruses can positively and negatively contribute to a host's physiology (including important human and plant pathogens), the presence of partitiviruses in S. cerevisiae offers a unique opportunity to study the biology of these viruses in a well-developed model system. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T12:01:23Z 2023-07-29T12:01:23Z 2023-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.ppat.1011418 Plos Pathogens. San Francisco: Public Library Science, v. 19, n. 6, 22 p., 2023. 1553-7366 http://hdl.handle.net/11449/245662 10.1371/journal.ppat.1011418 WOS:001003237700002 |
url |
http://dx.doi.org/10.1371/journal.ppat.1011418 http://hdl.handle.net/11449/245662 |
identifier_str_mv |
Plos Pathogens. San Francisco: Public Library Science, v. 19, n. 6, 22 p., 2023. 1553-7366 10.1371/journal.ppat.1011418 WOS:001003237700002 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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Plos Pathogens |
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info:eu-repo/semantics/openAccess |
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openAccess |
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22 |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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