Metalloproteomic profile determination of muscle samples from nile Tilapia (Oreochromis niloticus) using AAS and ESI-MS/MS after 2D-PAGE separation
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015000200239&lng=en&nrm=iso&tlng=en http://hdl.handle.net/11449/128655 |
Resumo: | This study evaluated the metalloproteomic profile of muscle tissue samples from Nile tilapia (Oreochromis niloticus). Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) methods was used for protein fractionation and identification based on image analysis. Determination of Ca, Cu, Fe, Mn and Zn was performed in the protein spots by flame and/or graphite furnace atomic absorption spectrometry (FAAS, GFAAS), the protein spots were characterized by electrospray ionization-tandem mass spectrometry (ESI-MS/MS). FAAS and GFAAS determinations have indicated the presence of calcium in seven protein spots, iron in only one spot, manganese in six spots, and zinc in two spots. Of the seventeen protein spots where the presence of metal ions was detected, ten were characterized by ESI-MS/MS. |
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Repositório Institucional da UNESP |
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Metalloproteomic profile determination of muscle samples from nile Tilapia (Oreochromis niloticus) using AAS and ESI-MS/MS after 2D-PAGE separationMetalloproteinsESI-MS/MS2D-PAGEThis study evaluated the metalloproteomic profile of muscle tissue samples from Nile tilapia (Oreochromis niloticus). Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) methods was used for protein fractionation and identification based on image analysis. Determination of Ca, Cu, Fe, Mn and Zn was performed in the protein spots by flame and/or graphite furnace atomic absorption spectrometry (FAAS, GFAAS), the protein spots were characterized by electrospray ionization-tandem mass spectrometry (ESI-MS/MS). FAAS and GFAAS determinations have indicated the presence of calcium in seven protein spots, iron in only one spot, manganese in six spots, and zinc in two spots. Of the seventeen protein spots where the presence of metal ions was detected, ten were characterized by ESI-MS/MS.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESP, Coll Vet &Anim Sci, BR-18618970 Botucatu, SP, BrazilUNESP, Inst Biosci, Dept Chem &Biochem, BR-18618970 Botucatu, SP, BrazilUNESP, Dept Biol Sci, BR-18618970 Botucatu, SP, BrazilUNESP, Coll Vet &Anim Sci, BR-18618970 Botucatu, SP, BrazilUNESP, Inst Biosci, Dept Chem &Biochem, BR-18618970 Botucatu, SP, BrazilUNESP, Dept Biol Sci, BR-18618970 Botucatu, SP, BrazilFAPESP: 2011/14902-0FAPESP: 2010/51332-5Soc Brasileira QuimicaUniversidade Estadual Paulista (Unesp)Cavecci, Bruna [UNESP]Lima, Paula M. de [UNESP]Queiroz, Joao V. de [UNESP]Braga, Camila P. [UNESP]Padilha, Cilene C. F. [UNESP]Leite, Aline L. [UNESP]Buzalaf, Marilia A. R. [UNESP]Pezzato, Luiz E. [UNESP]Padilha, Pedro M. [UNESP]2015-10-21T13:11:57Z2015-10-21T13:11:57Z2015-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article239-246application/pdfhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015000200239&lng=en&nrm=iso&tlng=enJournal Of The Brazilian Chemical Society, v. 26, n. 2, p. 239-246, 2015.0103-5053http://hdl.handle.net/11449/12865510.5935/0103-5053.20140260S0103-50532015000200239WOS:000351850100005S0103-50532015000200239.pdf69814486374563910000-0003-4179-0574Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of The Brazilian Chemical Society1.4440,357info:eu-repo/semantics/openAccess2023-12-03T06:12:54Zoai:repositorio.unesp.br:11449/128655Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:21:53.114765Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Metalloproteomic profile determination of muscle samples from nile Tilapia (Oreochromis niloticus) using AAS and ESI-MS/MS after 2D-PAGE separation |
title |
Metalloproteomic profile determination of muscle samples from nile Tilapia (Oreochromis niloticus) using AAS and ESI-MS/MS after 2D-PAGE separation |
spellingShingle |
Metalloproteomic profile determination of muscle samples from nile Tilapia (Oreochromis niloticus) using AAS and ESI-MS/MS after 2D-PAGE separation Cavecci, Bruna [UNESP] Metalloproteins ESI-MS/MS 2D-PAGE |
title_short |
Metalloproteomic profile determination of muscle samples from nile Tilapia (Oreochromis niloticus) using AAS and ESI-MS/MS after 2D-PAGE separation |
title_full |
Metalloproteomic profile determination of muscle samples from nile Tilapia (Oreochromis niloticus) using AAS and ESI-MS/MS after 2D-PAGE separation |
title_fullStr |
Metalloproteomic profile determination of muscle samples from nile Tilapia (Oreochromis niloticus) using AAS and ESI-MS/MS after 2D-PAGE separation |
title_full_unstemmed |
Metalloproteomic profile determination of muscle samples from nile Tilapia (Oreochromis niloticus) using AAS and ESI-MS/MS after 2D-PAGE separation |
title_sort |
Metalloproteomic profile determination of muscle samples from nile Tilapia (Oreochromis niloticus) using AAS and ESI-MS/MS after 2D-PAGE separation |
author |
Cavecci, Bruna [UNESP] |
author_facet |
Cavecci, Bruna [UNESP] Lima, Paula M. de [UNESP] Queiroz, Joao V. de [UNESP] Braga, Camila P. [UNESP] Padilha, Cilene C. F. [UNESP] Leite, Aline L. [UNESP] Buzalaf, Marilia A. R. [UNESP] Pezzato, Luiz E. [UNESP] Padilha, Pedro M. [UNESP] |
author_role |
author |
author2 |
Lima, Paula M. de [UNESP] Queiroz, Joao V. de [UNESP] Braga, Camila P. [UNESP] Padilha, Cilene C. F. [UNESP] Leite, Aline L. [UNESP] Buzalaf, Marilia A. R. [UNESP] Pezzato, Luiz E. [UNESP] Padilha, Pedro M. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Cavecci, Bruna [UNESP] Lima, Paula M. de [UNESP] Queiroz, Joao V. de [UNESP] Braga, Camila P. [UNESP] Padilha, Cilene C. F. [UNESP] Leite, Aline L. [UNESP] Buzalaf, Marilia A. R. [UNESP] Pezzato, Luiz E. [UNESP] Padilha, Pedro M. [UNESP] |
dc.subject.por.fl_str_mv |
Metalloproteins ESI-MS/MS 2D-PAGE |
topic |
Metalloproteins ESI-MS/MS 2D-PAGE |
description |
This study evaluated the metalloproteomic profile of muscle tissue samples from Nile tilapia (Oreochromis niloticus). Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) methods was used for protein fractionation and identification based on image analysis. Determination of Ca, Cu, Fe, Mn and Zn was performed in the protein spots by flame and/or graphite furnace atomic absorption spectrometry (FAAS, GFAAS), the protein spots were characterized by electrospray ionization-tandem mass spectrometry (ESI-MS/MS). FAAS and GFAAS determinations have indicated the presence of calcium in seven protein spots, iron in only one spot, manganese in six spots, and zinc in two spots. Of the seventeen protein spots where the presence of metal ions was detected, ten were characterized by ESI-MS/MS. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-21T13:11:57Z 2015-10-21T13:11:57Z 2015-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015000200239&lng=en&nrm=iso&tlng=en Journal Of The Brazilian Chemical Society, v. 26, n. 2, p. 239-246, 2015. 0103-5053 http://hdl.handle.net/11449/128655 10.5935/0103-5053.20140260 S0103-50532015000200239 WOS:000351850100005 S0103-50532015000200239.pdf 6981448637456391 0000-0003-4179-0574 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015000200239&lng=en&nrm=iso&tlng=en http://hdl.handle.net/11449/128655 |
identifier_str_mv |
Journal Of The Brazilian Chemical Society, v. 26, n. 2, p. 239-246, 2015. 0103-5053 10.5935/0103-5053.20140260 S0103-50532015000200239 WOS:000351850100005 S0103-50532015000200239.pdf 6981448637456391 0000-0003-4179-0574 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of The Brazilian Chemical Society 1.444 0,357 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
239-246 application/pdf |
dc.publisher.none.fl_str_mv |
Soc Brasileira Quimica |
publisher.none.fl_str_mv |
Soc Brasileira Quimica |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129059966681088 |