Mello and Campos (1974) method adapted for the recovery of cestodes in birds ( Gallus domesticus )

Detalhes bibliográficos
Autor(a) principal: Silva, Giane Serafim Da
Data de Publicação: 2016
Outros Autores: Romera, Daiane Mompean, Prado, Maykon Bichoffi Do, Soares, Vando Edésio, Meireles, Marcelo Vasconcelos [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1808-1657000752015
http://hdl.handle.net/11449/213201
Resumo: The specific diagnosis and evaluation of the intensity of avian helminth infections are essential for efficacy studies and the determination of drug doses targeted to their control. This study evaluated the Mello and Campos method, originally described for parasitological diagnosis in dogs, in the recovery of scolices from cestode parasites of poultry (Gallus domesticus ). A total of 52 naturally infected birds obtained from farms underwent parasitological necropsy using the Mello and Campos method. The method consisted of four steps: content, soaking, scraping and evaluation. The number of scolices recovered per bird ranged from 1 to 4,345, and the highest number of scolices was recovered from material derived from the soaking step. The cestodes species diagnosed were Amoebotaenia cuneata , Choanotaenia infundibulum , Hymenolepis sp., Raillietina tetragona , Raillietina echinobothrida and Raillietina cesticillus . The Mello and Campos method, originally used to test for helminths in dogs, was effective in avian cestode testing because it includes a soaking step, which enables a more efficient recovery of scolices.
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spelling Mello and Campos (1974) method adapted for the recovery of cestodes in birds ( Gallus domesticus )Método Mello e Campos (1974) adaptado para recuperação de cestódeos de aves (Gallus domesticus)Gallus domesticusbirdscestodahelminthsMello and CamposGallus domesticusavescestódeoshelmintosMello e CamposThe specific diagnosis and evaluation of the intensity of avian helminth infections are essential for efficacy studies and the determination of drug doses targeted to their control. This study evaluated the Mello and Campos method, originally described for parasitological diagnosis in dogs, in the recovery of scolices from cestode parasites of poultry (Gallus domesticus ). A total of 52 naturally infected birds obtained from farms underwent parasitological necropsy using the Mello and Campos method. The method consisted of four steps: content, soaking, scraping and evaluation. The number of scolices recovered per bird ranged from 1 to 4,345, and the highest number of scolices was recovered from material derived from the soaking step. The cestodes species diagnosed were Amoebotaenia cuneata , Choanotaenia infundibulum , Hymenolepis sp., Raillietina tetragona , Raillietina echinobothrida and Raillietina cesticillus . The Mello and Campos method, originally used to test for helminths in dogs, was effective in avian cestode testing because it includes a soaking step, which enables a more efficient recovery of scolices.O diagnóstico específico e a avaliação da intensidade da infecção helmíntica em aves são fundamentais em estudos de eficácia e determinação de doses de medicamentos direcionados ao seu controle. O presente trabalho avaliou a aplicação e adaptação da metodologia de Mello e Campos, descrita originalmente para diagnóstico parasitológico em cães, na recuperação de escólices de cestódeos parasitos de aves domésticas (Gallus domesticus ). Foram empregadas 52 aves naturalmente infectadas e oriundas de produções rurais, as quais foram submetidas à necropsia parasitológica, adaptando-se a metodologia Mello e Campos. O método consistiu na realização de quatro etapas: conteúdo, imersão, raspado e avaliação. O número de escólices recuperadas por ave variou de 1 a 4.345, e o maior número de escólices foi recuperado do material oriundo da etapa de imersão. As espécies de cestódeos identificadas foram Amoebotaenia cuneata , Choanotaenia infundibulum , Hymenolepis sp., Raillietina tetragona , Raillietina echinobothrida e Raillietina cesticillus . Os resultados foram avaliados estatisticamente, concluindo-se que a metodologia adotada é eficaz para a recuperação de cestódeos de aves, uma vez que possui a etapa de imersão, que permite a recuperação mais eficiente de escólices.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Instituto Biológico, Laboratório de Parasitologia AnimalInstituto Agronômico de CampinasUniversidade Camilo Castelo BrancoUniversidade Estadual Paulista Júlio de Mesquita Filho, School of Veterinary MedicineUniversidade Estadual Paulista Júlio de Mesquita Filho, School of Veterinary MedicineFAPESP: 2012/18929-3; 2013/03599-0; 2014/04097-1Instituto BiológicoInstituto BiológicoInstituto Agronômico de CampinasUniversidade Camilo Castelo BrancoUniversidade Estadual Paulista (Unesp)Silva, Giane Serafim DaRomera, Daiane MompeanPrado, Maykon Bichoffi DoSoares, Vando EdésioMeireles, Marcelo Vasconcelos [UNESP]2021-07-14T10:51:34Z2021-07-14T10:51:34Z2016-12-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article-application/pdfhttp://dx.doi.org/10.1590/1808-1657000752015Arquivos do Instituto Biológico. Instituto Biológico, v. 83, p. -, 2016.1808-1657http://hdl.handle.net/11449/21320110.1590/1808-1657000752015S1808-16572016000100233S1808-16572016000100233.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivos do Instituto Biológicoinfo:eu-repo/semantics/openAccess2024-09-04T18:04:10Zoai:repositorio.unesp.br:11449/213201Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T18:04:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Mello and Campos (1974) method adapted for the recovery of cestodes in birds ( Gallus domesticus )
Método Mello e Campos (1974) adaptado para recuperação de cestódeos de aves (Gallus domesticus)
title Mello and Campos (1974) method adapted for the recovery of cestodes in birds ( Gallus domesticus )
spellingShingle Mello and Campos (1974) method adapted for the recovery of cestodes in birds ( Gallus domesticus )
Silva, Giane Serafim Da
Gallus domesticus
birds
cestoda
helminths
Mello and Campos
Gallus domesticus
aves
cestódeos
helmintos
Mello e Campos
title_short Mello and Campos (1974) method adapted for the recovery of cestodes in birds ( Gallus domesticus )
title_full Mello and Campos (1974) method adapted for the recovery of cestodes in birds ( Gallus domesticus )
title_fullStr Mello and Campos (1974) method adapted for the recovery of cestodes in birds ( Gallus domesticus )
title_full_unstemmed Mello and Campos (1974) method adapted for the recovery of cestodes in birds ( Gallus domesticus )
title_sort Mello and Campos (1974) method adapted for the recovery of cestodes in birds ( Gallus domesticus )
author Silva, Giane Serafim Da
author_facet Silva, Giane Serafim Da
Romera, Daiane Mompean
Prado, Maykon Bichoffi Do
Soares, Vando Edésio
Meireles, Marcelo Vasconcelos [UNESP]
author_role author
author2 Romera, Daiane Mompean
Prado, Maykon Bichoffi Do
Soares, Vando Edésio
Meireles, Marcelo Vasconcelos [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Instituto Biológico
Instituto Agronômico de Campinas
Universidade Camilo Castelo Branco
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Silva, Giane Serafim Da
Romera, Daiane Mompean
Prado, Maykon Bichoffi Do
Soares, Vando Edésio
Meireles, Marcelo Vasconcelos [UNESP]
dc.subject.por.fl_str_mv Gallus domesticus
birds
cestoda
helminths
Mello and Campos
Gallus domesticus
aves
cestódeos
helmintos
Mello e Campos
topic Gallus domesticus
birds
cestoda
helminths
Mello and Campos
Gallus domesticus
aves
cestódeos
helmintos
Mello e Campos
description The specific diagnosis and evaluation of the intensity of avian helminth infections are essential for efficacy studies and the determination of drug doses targeted to their control. This study evaluated the Mello and Campos method, originally described for parasitological diagnosis in dogs, in the recovery of scolices from cestode parasites of poultry (Gallus domesticus ). A total of 52 naturally infected birds obtained from farms underwent parasitological necropsy using the Mello and Campos method. The method consisted of four steps: content, soaking, scraping and evaluation. The number of scolices recovered per bird ranged from 1 to 4,345, and the highest number of scolices was recovered from material derived from the soaking step. The cestodes species diagnosed were Amoebotaenia cuneata , Choanotaenia infundibulum , Hymenolepis sp., Raillietina tetragona , Raillietina echinobothrida and Raillietina cesticillus . The Mello and Campos method, originally used to test for helminths in dogs, was effective in avian cestode testing because it includes a soaking step, which enables a more efficient recovery of scolices.
publishDate 2016
dc.date.none.fl_str_mv 2016-12-15
2021-07-14T10:51:34Z
2021-07-14T10:51:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1808-1657000752015
Arquivos do Instituto Biológico. Instituto Biológico, v. 83, p. -, 2016.
1808-1657
http://hdl.handle.net/11449/213201
10.1590/1808-1657000752015
S1808-16572016000100233
S1808-16572016000100233.pdf
url http://dx.doi.org/10.1590/1808-1657000752015
http://hdl.handle.net/11449/213201
identifier_str_mv Arquivos do Instituto Biológico. Instituto Biológico, v. 83, p. -, 2016.
1808-1657
10.1590/1808-1657000752015
S1808-16572016000100233
S1808-16572016000100233.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivos do Instituto Biológico
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv -
application/pdf
dc.publisher.none.fl_str_mv Instituto Biológico
publisher.none.fl_str_mv Instituto Biológico
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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