MicroRNA-10 modulates Hox genes expression during Nile tilapia embryonic development

Detalhes bibliográficos
Autor(a) principal: Giusti, Juliana [UNESP]
Data de Publicação: 2016
Outros Autores: Pinhal, Danillo [UNESP], Moxon, Simon, Campos, Camila Lovaglio [UNESP], Munsterberg, Andrea, Martins, Cesar [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.mod.2016.03.002
http://hdl.handle.net/11449/161539
Resumo: Hox gene clusters encode a family of transcription factors that govern anterior-posterior axis patterning during embryogenesis in all bilaterian animals. The time and place of Hox gene expression are largely determined by the relative position of each gene within its cluster. Furthermore, Hox genes were shown to have their expression fine-tuned by regulatory microRNAs (miRNAs). However, the mechanisms of miRNA-mediated regulation of these transcription factors during fish early development remain largely unknown. Here we have profiled three highly expressed miR-10 family members of Nile tilapia at early embryonic development, determined their genomic organization as well as performed functional experiments for validation of target genes. Quantitative analysis during developmental stages showed miR-10 family expression negatively correlates with the expression of HoxA3a, HoxB3a and HoxD10a genes, as expected for bona fide miRNA-mRNA interactions. Moreover, luciferase assays demonstrated that HoxB3a and HoxD10a are targeted by miR-10b-5p. Overall, our data indicate that the miR-10 family directly regulates members of the Hox gene family during Nile tilapia embryogenesis. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
id UNSP_b59238b3513047f2d0106a83b2d61967
oai_identifier_str oai:repositorio.unesp.br:11449/161539
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling MicroRNA-10 modulates Hox genes expression during Nile tilapia embryonic developmentOreochromis niloticusEmbryosHoxA3aHoxB3aHoxD10amiRNAHox gene clusters encode a family of transcription factors that govern anterior-posterior axis patterning during embryogenesis in all bilaterian animals. The time and place of Hox gene expression are largely determined by the relative position of each gene within its cluster. Furthermore, Hox genes were shown to have their expression fine-tuned by regulatory microRNAs (miRNAs). However, the mechanisms of miRNA-mediated regulation of these transcription factors during fish early development remain largely unknown. Here we have profiled three highly expressed miR-10 family members of Nile tilapia at early embryonic development, determined their genomic organization as well as performed functional experiments for validation of target genes. Quantitative analysis during developmental stages showed miR-10 family expression negatively correlates with the expression of HoxA3a, HoxB3a and HoxD10a genes, as expected for bona fide miRNA-mRNA interactions. Moreover, luciferase assays demonstrated that HoxB3a and HoxD10a are targeted by miR-10b-5p. Overall, our data indicate that the miR-10 family directly regulates members of the Hox gene family during Nile tilapia embryogenesis. (C) 2016 Elsevier Ireland Ltd. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Paulo State Univ, Dept Morphol, Inst Biosci, Botucatu, SP, BrazilSao Paulo State Univ, Dept Genet, Inst Biosci, Botucatu, SP, BrazilGenome Anal Ctr, Norwich Res Pk, Norwich, Norfolk, EnglandUniv E Anglia, Sch Biol Sci, Norwich Res Pk, Norwich NR4 7TJ, Norfolk, EnglandSao Paulo State Univ, Dept Morphol, Inst Biosci, Botucatu, SP, BrazilSao Paulo State Univ, Dept Genet, Inst Biosci, Botucatu, SP, BrazilFAPESP: 2011/06465-0CAPES: 18311/12-1Elsevier B.V.Universidade Estadual Paulista (Unesp)Genome Anal CtrUniv E AngliaGiusti, Juliana [UNESP]Pinhal, Danillo [UNESP]Moxon, SimonCampos, Camila Lovaglio [UNESP]Munsterberg, AndreaMartins, Cesar [UNESP]2018-11-26T16:33:09Z2018-11-26T16:33:09Z2016-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12-18application/pdfhttp://dx.doi.org/10.1016/j.mod.2016.03.002Mechanisms Of Development. Amsterdam: Elsevier Science Bv, v. 140, p. 12-18, 2016.0925-4773http://hdl.handle.net/11449/16153910.1016/j.mod.2016.03.002WOS:000376545000002WOS000376545000002.pdf88588006994253520000-0003-3534-974XWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMechanisms Of Development1,049info:eu-repo/semantics/openAccess2023-11-02T06:09:00Zoai:repositorio.unesp.br:11449/161539Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:42:57.562528Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv MicroRNA-10 modulates Hox genes expression during Nile tilapia embryonic development
title MicroRNA-10 modulates Hox genes expression during Nile tilapia embryonic development
spellingShingle MicroRNA-10 modulates Hox genes expression during Nile tilapia embryonic development
Giusti, Juliana [UNESP]
Oreochromis niloticus
Embryos
HoxA3a
HoxB3a
HoxD10a
miRNA
title_short MicroRNA-10 modulates Hox genes expression during Nile tilapia embryonic development
title_full MicroRNA-10 modulates Hox genes expression during Nile tilapia embryonic development
title_fullStr MicroRNA-10 modulates Hox genes expression during Nile tilapia embryonic development
title_full_unstemmed MicroRNA-10 modulates Hox genes expression during Nile tilapia embryonic development
title_sort MicroRNA-10 modulates Hox genes expression during Nile tilapia embryonic development
author Giusti, Juliana [UNESP]
author_facet Giusti, Juliana [UNESP]
Pinhal, Danillo [UNESP]
Moxon, Simon
Campos, Camila Lovaglio [UNESP]
Munsterberg, Andrea
Martins, Cesar [UNESP]
author_role author
author2 Pinhal, Danillo [UNESP]
Moxon, Simon
Campos, Camila Lovaglio [UNESP]
Munsterberg, Andrea
Martins, Cesar [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Genome Anal Ctr
Univ E Anglia
dc.contributor.author.fl_str_mv Giusti, Juliana [UNESP]
Pinhal, Danillo [UNESP]
Moxon, Simon
Campos, Camila Lovaglio [UNESP]
Munsterberg, Andrea
Martins, Cesar [UNESP]
dc.subject.por.fl_str_mv Oreochromis niloticus
Embryos
HoxA3a
HoxB3a
HoxD10a
miRNA
topic Oreochromis niloticus
Embryos
HoxA3a
HoxB3a
HoxD10a
miRNA
description Hox gene clusters encode a family of transcription factors that govern anterior-posterior axis patterning during embryogenesis in all bilaterian animals. The time and place of Hox gene expression are largely determined by the relative position of each gene within its cluster. Furthermore, Hox genes were shown to have their expression fine-tuned by regulatory microRNAs (miRNAs). However, the mechanisms of miRNA-mediated regulation of these transcription factors during fish early development remain largely unknown. Here we have profiled three highly expressed miR-10 family members of Nile tilapia at early embryonic development, determined their genomic organization as well as performed functional experiments for validation of target genes. Quantitative analysis during developmental stages showed miR-10 family expression negatively correlates with the expression of HoxA3a, HoxB3a and HoxD10a genes, as expected for bona fide miRNA-mRNA interactions. Moreover, luciferase assays demonstrated that HoxB3a and HoxD10a are targeted by miR-10b-5p. Overall, our data indicate that the miR-10 family directly regulates members of the Hox gene family during Nile tilapia embryogenesis. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
publishDate 2016
dc.date.none.fl_str_mv 2016-05-01
2018-11-26T16:33:09Z
2018-11-26T16:33:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.mod.2016.03.002
Mechanisms Of Development. Amsterdam: Elsevier Science Bv, v. 140, p. 12-18, 2016.
0925-4773
http://hdl.handle.net/11449/161539
10.1016/j.mod.2016.03.002
WOS:000376545000002
WOS000376545000002.pdf
8858800699425352
0000-0003-3534-974X
url http://dx.doi.org/10.1016/j.mod.2016.03.002
http://hdl.handle.net/11449/161539
identifier_str_mv Mechanisms Of Development. Amsterdam: Elsevier Science Bv, v. 140, p. 12-18, 2016.
0925-4773
10.1016/j.mod.2016.03.002
WOS:000376545000002
WOS000376545000002.pdf
8858800699425352
0000-0003-3534-974X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Mechanisms Of Development
1,049
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12-18
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128690956009472