Acute hypercortisolemia inhibits innate immune parameters in Piaractus mesopotamicus experimentally infected with Aeromonas hydrophila
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.aquaculture.2020.735231 http://hdl.handle.net/11449/200191 |
Resumo: | Cortisol is a key modulator of the immune response in fish, and considered an essential molecule for adaptation of animals to stress and overcoming the stressor. Antagonist effects, pro and anti-inflammatory have been associated to acute increases in cortisol levels, however knowledge about the mechanisms of this regulation in tropical teleosts is scarce. The effects of pharmacological inhibition of cortisol release with metyrapone (MTP) on innate immune response were investigated for the freshwater teleost, Piaractus mesopotamicus, using Aeromonas hydrophila to induce an inflammatory-immune response. Pacus were randomly distributed into four experimental groups. T1: basal control, without any previous handling. T2: without MTP treatment, inoculated via swim bladder with lethal concentration 50%-96 h (LC50- 96h) of A. hydrophila. T3: supplemented with 30mgkg−1 MTP in the diet for five days, injected on the sixth day with 50mgkg−1 live weight (LW) MTP and immediately inoculated with the bacteria. T4: supplemented with MTP, without bacterial inoculation. Blood samples were taken at 1, 3, 6 and 9 h post infection (hpi), for assessment of cortisolemia, glycemia, hematological and innate immunity parameters. The results revealed that high cortisol concentrations, during the initial phase of A. hydrophila infection, inhibited the immune-inflammatory response. Fish treated with MTP and inoculated with the bacterium showed significant higher total blood leukocytes and lymphocytes numbers, blood phagocyte respiratory activity and plasma lytic activity when compare to infected fish that did not receive MTP. It was concluded that the inhibition of cortisol release favored the immune-inflammatory response during the initial phase of bacterial infection. |
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Repositório Institucional da UNESP |
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Acute hypercortisolemia inhibits innate immune parameters in Piaractus mesopotamicus experimentally infected with Aeromonas hydrophilaCortisolInflammationMetyraponePacuSepsisCortisol is a key modulator of the immune response in fish, and considered an essential molecule for adaptation of animals to stress and overcoming the stressor. Antagonist effects, pro and anti-inflammatory have been associated to acute increases in cortisol levels, however knowledge about the mechanisms of this regulation in tropical teleosts is scarce. The effects of pharmacological inhibition of cortisol release with metyrapone (MTP) on innate immune response were investigated for the freshwater teleost, Piaractus mesopotamicus, using Aeromonas hydrophila to induce an inflammatory-immune response. Pacus were randomly distributed into four experimental groups. T1: basal control, without any previous handling. T2: without MTP treatment, inoculated via swim bladder with lethal concentration 50%-96 h (LC50- 96h) of A. hydrophila. T3: supplemented with 30mgkg−1 MTP in the diet for five days, injected on the sixth day with 50mgkg−1 live weight (LW) MTP and immediately inoculated with the bacteria. T4: supplemented with MTP, without bacterial inoculation. Blood samples were taken at 1, 3, 6 and 9 h post infection (hpi), for assessment of cortisolemia, glycemia, hematological and innate immunity parameters. The results revealed that high cortisol concentrations, during the initial phase of A. hydrophila infection, inhibited the immune-inflammatory response. Fish treated with MTP and inoculated with the bacterium showed significant higher total blood leukocytes and lymphocytes numbers, blood phagocyte respiratory activity and plasma lytic activity when compare to infected fish that did not receive MTP. It was concluded that the inhibition of cortisol release favored the immune-inflammatory response during the initial phase of bacterial infection.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Aquaculture Center of Unesp (Caunesp) Sao Paulo State University (Unesp), Via Prof. Paulo Donato Castellane, km 05, 14Laboratory of Aquatic Pathobiology Sea Institute of Peru (IMARPE), Esquina Gamarra y General Valle S/NDepartment of Veterinary Pathology School of Agricultural and Veterinary Studies (FCAV), Unesp. Via Prof. Paulo Donato Castellane, km 05, 14Aquaculture Center of Unesp (Caunesp) Sao Paulo State University (Unesp), Via Prof. Paulo Donato Castellane, km 05, 14CNPq: 141835/2018-4FAPESP: 2015/14289-8FAPESP: 2016/18345-2CNPq: 446917/2014-1Universidade Estadual Paulista (Unesp)Sea Institute of Peru (IMARPE)Cueva-Quiroz, Victor Alexander [UNESP]Yunis-Aguinaga, Jefferson [UNESP]Ramos-Espinoza, Fernando Carlos [UNESP]Ruas de Moraes, FlávioEngrácia de Moraes, Julieta Rodini [UNESP]2020-12-12T02:00:03Z2020-12-12T02:00:03Z2020-06-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.aquaculture.2020.735231Aquaculture, v. 523.0044-8486http://hdl.handle.net/11449/20019110.1016/j.aquaculture.2020.7352312-s2.0-85081958466Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquacultureinfo:eu-repo/semantics/openAccess2024-04-09T15:43:44Zoai:repositorio.unesp.br:11449/200191Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:43:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Acute hypercortisolemia inhibits innate immune parameters in Piaractus mesopotamicus experimentally infected with Aeromonas hydrophila |
title |
Acute hypercortisolemia inhibits innate immune parameters in Piaractus mesopotamicus experimentally infected with Aeromonas hydrophila |
spellingShingle |
Acute hypercortisolemia inhibits innate immune parameters in Piaractus mesopotamicus experimentally infected with Aeromonas hydrophila Cueva-Quiroz, Victor Alexander [UNESP] Cortisol Inflammation Metyrapone Pacu Sepsis |
title_short |
Acute hypercortisolemia inhibits innate immune parameters in Piaractus mesopotamicus experimentally infected with Aeromonas hydrophila |
title_full |
Acute hypercortisolemia inhibits innate immune parameters in Piaractus mesopotamicus experimentally infected with Aeromonas hydrophila |
title_fullStr |
Acute hypercortisolemia inhibits innate immune parameters in Piaractus mesopotamicus experimentally infected with Aeromonas hydrophila |
title_full_unstemmed |
Acute hypercortisolemia inhibits innate immune parameters in Piaractus mesopotamicus experimentally infected with Aeromonas hydrophila |
title_sort |
Acute hypercortisolemia inhibits innate immune parameters in Piaractus mesopotamicus experimentally infected with Aeromonas hydrophila |
author |
Cueva-Quiroz, Victor Alexander [UNESP] |
author_facet |
Cueva-Quiroz, Victor Alexander [UNESP] Yunis-Aguinaga, Jefferson [UNESP] Ramos-Espinoza, Fernando Carlos [UNESP] Ruas de Moraes, Flávio Engrácia de Moraes, Julieta Rodini [UNESP] |
author_role |
author |
author2 |
Yunis-Aguinaga, Jefferson [UNESP] Ramos-Espinoza, Fernando Carlos [UNESP] Ruas de Moraes, Flávio Engrácia de Moraes, Julieta Rodini [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Sea Institute of Peru (IMARPE) |
dc.contributor.author.fl_str_mv |
Cueva-Quiroz, Victor Alexander [UNESP] Yunis-Aguinaga, Jefferson [UNESP] Ramos-Espinoza, Fernando Carlos [UNESP] Ruas de Moraes, Flávio Engrácia de Moraes, Julieta Rodini [UNESP] |
dc.subject.por.fl_str_mv |
Cortisol Inflammation Metyrapone Pacu Sepsis |
topic |
Cortisol Inflammation Metyrapone Pacu Sepsis |
description |
Cortisol is a key modulator of the immune response in fish, and considered an essential molecule for adaptation of animals to stress and overcoming the stressor. Antagonist effects, pro and anti-inflammatory have been associated to acute increases in cortisol levels, however knowledge about the mechanisms of this regulation in tropical teleosts is scarce. The effects of pharmacological inhibition of cortisol release with metyrapone (MTP) on innate immune response were investigated for the freshwater teleost, Piaractus mesopotamicus, using Aeromonas hydrophila to induce an inflammatory-immune response. Pacus were randomly distributed into four experimental groups. T1: basal control, without any previous handling. T2: without MTP treatment, inoculated via swim bladder with lethal concentration 50%-96 h (LC50- 96h) of A. hydrophila. T3: supplemented with 30mgkg−1 MTP in the diet for five days, injected on the sixth day with 50mgkg−1 live weight (LW) MTP and immediately inoculated with the bacteria. T4: supplemented with MTP, without bacterial inoculation. Blood samples were taken at 1, 3, 6 and 9 h post infection (hpi), for assessment of cortisolemia, glycemia, hematological and innate immunity parameters. The results revealed that high cortisol concentrations, during the initial phase of A. hydrophila infection, inhibited the immune-inflammatory response. Fish treated with MTP and inoculated with the bacterium showed significant higher total blood leukocytes and lymphocytes numbers, blood phagocyte respiratory activity and plasma lytic activity when compare to infected fish that did not receive MTP. It was concluded that the inhibition of cortisol release favored the immune-inflammatory response during the initial phase of bacterial infection. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:00:03Z 2020-12-12T02:00:03Z 2020-06-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.aquaculture.2020.735231 Aquaculture, v. 523. 0044-8486 http://hdl.handle.net/11449/200191 10.1016/j.aquaculture.2020.735231 2-s2.0-85081958466 |
url |
http://dx.doi.org/10.1016/j.aquaculture.2020.735231 http://hdl.handle.net/11449/200191 |
identifier_str_mv |
Aquaculture, v. 523. 0044-8486 10.1016/j.aquaculture.2020.735231 2-s2.0-85081958466 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Aquaculture |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965731247357952 |