Physicochemical, biological, and antibacterial evaluation of tricalcium silicate-based reparative cements with different radiopacifiers
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.dental.2020.11.014 http://hdl.handle.net/11449/205591 |
Resumo: | Objective: To evaluate tricalcium silicate-based (TCS) experimental materials, associated with zirconium oxide (ZrO2), calcium tungstate (CaWO4) or niobium oxide (Nb2O5) radiopacifiers, in comparison with MTA Repair HP (Angelus). Methods: Physicochemical tests: setting time, radiopacity, pH and solubility. In vitro assays: cytotoxicity: MTT and Neutral Red – NR; cell bioactivity: alkaline phosphatase activity (ALP), Alzarin red staining (ARS) and real time PCR (qPCR). Antibacterial activity: direct contact on Enterococcus faecalis in the planktonic form. Physicochemical and ARS data were submitted to ANOVA/Tukey tests; antibacterial activity, to Kruskall–Wallis and Dunn tests; MTT, NR, ALP and qPCR were analyzed by ANOVA/Bonferroni tests (α = 0.05). Results: TCS + CaWO4 presented the longest setting time and MTA HP the shortest. Except for TCS, all the materials presented radiopacity above 3 mmAl. The cements had alkaline pH, antibacterial activity, low solubility and no cytotoxic effects. The highest ALP activity occurred in 14 days, especially to TCS, TCS + ZrO2 and TCS + CaWO4. TCS + ZrO2, TCS + Nb2O5 and MTAHP had higher mineralized nodule formation than those of the negative control (NC). After 7 days, there was no difference in mRNA expression for ALP, when compared to NC. However, after 14 days there was no overexpressed ALP mRNA, especially TCS + Nb2O5, in relation to the CN. All the materials presented antimicrobial action. Significance: The pure tricalcium silicate associated with ZrO2, CaWO4 or Nb2O5 had appropriate physicochemical properties, antibacterial activity, cytocompatibility and induced mineralization in Saos-2, indicating their use as reparative materials. |
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Physicochemical, biological, and antibacterial evaluation of tricalcium silicate-based reparative cements with different radiopacifiersBiological propertiesCalcium silicateChemical propertiesDental materialsEndodonticsPhysical propertiesObjective: To evaluate tricalcium silicate-based (TCS) experimental materials, associated with zirconium oxide (ZrO2), calcium tungstate (CaWO4) or niobium oxide (Nb2O5) radiopacifiers, in comparison with MTA Repair HP (Angelus). Methods: Physicochemical tests: setting time, radiopacity, pH and solubility. In vitro assays: cytotoxicity: MTT and Neutral Red – NR; cell bioactivity: alkaline phosphatase activity (ALP), Alzarin red staining (ARS) and real time PCR (qPCR). Antibacterial activity: direct contact on Enterococcus faecalis in the planktonic form. Physicochemical and ARS data were submitted to ANOVA/Tukey tests; antibacterial activity, to Kruskall–Wallis and Dunn tests; MTT, NR, ALP and qPCR were analyzed by ANOVA/Bonferroni tests (α = 0.05). Results: TCS + CaWO4 presented the longest setting time and MTA HP the shortest. Except for TCS, all the materials presented radiopacity above 3 mmAl. The cements had alkaline pH, antibacterial activity, low solubility and no cytotoxic effects. The highest ALP activity occurred in 14 days, especially to TCS, TCS + ZrO2 and TCS + CaWO4. TCS + ZrO2, TCS + Nb2O5 and MTAHP had higher mineralized nodule formation than those of the negative control (NC). After 7 days, there was no difference in mRNA expression for ALP, when compared to NC. However, after 14 days there was no overexpressed ALP mRNA, especially TCS + Nb2O5, in relation to the CN. All the materials presented antimicrobial action. Significance: The pure tricalcium silicate associated with ZrO2, CaWO4 or Nb2O5 had appropriate physicochemical properties, antibacterial activity, cytocompatibility and induced mineralization in Saos-2, indicating their use as reparative materials.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Restorative Dentistry São Paulo State University (UNESP) School of DentistryDepartment of Dentistry Bahia Federal University (UFBA) School of DentistryDepartment of Restorative Dentistry São Paulo State University (UNESP) School of DentistryUniversidade Estadual Paulista (Unesp)Universidade Federal da Bahia (UFBA)Queiroz, Marcela Borsatto [UNESP]Torres, Fernanda Ferrari Esteves [UNESP]Rodrigues, Elisandra Marcia [UNESP]Viola, Kennia Scapin [UNESP]Bosso-Martelo, RobertaChavez-Andrade, Gisselle Moraima [UNESP]Guerreiro-Tanomaru, Juliane Maria [UNESP]Tanomaru-Filho, Mario [UNESP]2021-06-25T10:18:00Z2021-06-25T10:18:00Z2021-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article311-320http://dx.doi.org/10.1016/j.dental.2020.11.014Dental Materials, v. 37, n. 2, p. 311-320, 2021.0109-5641http://hdl.handle.net/11449/20559110.1016/j.dental.2020.11.0142-s2.0-85097682413Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDental Materialsinfo:eu-repo/semantics/openAccess2021-10-23T15:01:25Zoai:repositorio.unesp.br:11449/205591Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:21:53.157017Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Physicochemical, biological, and antibacterial evaluation of tricalcium silicate-based reparative cements with different radiopacifiers |
title |
Physicochemical, biological, and antibacterial evaluation of tricalcium silicate-based reparative cements with different radiopacifiers |
spellingShingle |
Physicochemical, biological, and antibacterial evaluation of tricalcium silicate-based reparative cements with different radiopacifiers Queiroz, Marcela Borsatto [UNESP] Biological properties Calcium silicate Chemical properties Dental materials Endodontics Physical properties |
title_short |
Physicochemical, biological, and antibacterial evaluation of tricalcium silicate-based reparative cements with different radiopacifiers |
title_full |
Physicochemical, biological, and antibacterial evaluation of tricalcium silicate-based reparative cements with different radiopacifiers |
title_fullStr |
Physicochemical, biological, and antibacterial evaluation of tricalcium silicate-based reparative cements with different radiopacifiers |
title_full_unstemmed |
Physicochemical, biological, and antibacterial evaluation of tricalcium silicate-based reparative cements with different radiopacifiers |
title_sort |
Physicochemical, biological, and antibacterial evaluation of tricalcium silicate-based reparative cements with different radiopacifiers |
author |
Queiroz, Marcela Borsatto [UNESP] |
author_facet |
Queiroz, Marcela Borsatto [UNESP] Torres, Fernanda Ferrari Esteves [UNESP] Rodrigues, Elisandra Marcia [UNESP] Viola, Kennia Scapin [UNESP] Bosso-Martelo, Roberta Chavez-Andrade, Gisselle Moraima [UNESP] Guerreiro-Tanomaru, Juliane Maria [UNESP] Tanomaru-Filho, Mario [UNESP] |
author_role |
author |
author2 |
Torres, Fernanda Ferrari Esteves [UNESP] Rodrigues, Elisandra Marcia [UNESP] Viola, Kennia Scapin [UNESP] Bosso-Martelo, Roberta Chavez-Andrade, Gisselle Moraima [UNESP] Guerreiro-Tanomaru, Juliane Maria [UNESP] Tanomaru-Filho, Mario [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal da Bahia (UFBA) |
dc.contributor.author.fl_str_mv |
Queiroz, Marcela Borsatto [UNESP] Torres, Fernanda Ferrari Esteves [UNESP] Rodrigues, Elisandra Marcia [UNESP] Viola, Kennia Scapin [UNESP] Bosso-Martelo, Roberta Chavez-Andrade, Gisselle Moraima [UNESP] Guerreiro-Tanomaru, Juliane Maria [UNESP] Tanomaru-Filho, Mario [UNESP] |
dc.subject.por.fl_str_mv |
Biological properties Calcium silicate Chemical properties Dental materials Endodontics Physical properties |
topic |
Biological properties Calcium silicate Chemical properties Dental materials Endodontics Physical properties |
description |
Objective: To evaluate tricalcium silicate-based (TCS) experimental materials, associated with zirconium oxide (ZrO2), calcium tungstate (CaWO4) or niobium oxide (Nb2O5) radiopacifiers, in comparison with MTA Repair HP (Angelus). Methods: Physicochemical tests: setting time, radiopacity, pH and solubility. In vitro assays: cytotoxicity: MTT and Neutral Red – NR; cell bioactivity: alkaline phosphatase activity (ALP), Alzarin red staining (ARS) and real time PCR (qPCR). Antibacterial activity: direct contact on Enterococcus faecalis in the planktonic form. Physicochemical and ARS data were submitted to ANOVA/Tukey tests; antibacterial activity, to Kruskall–Wallis and Dunn tests; MTT, NR, ALP and qPCR were analyzed by ANOVA/Bonferroni tests (α = 0.05). Results: TCS + CaWO4 presented the longest setting time and MTA HP the shortest. Except for TCS, all the materials presented radiopacity above 3 mmAl. The cements had alkaline pH, antibacterial activity, low solubility and no cytotoxic effects. The highest ALP activity occurred in 14 days, especially to TCS, TCS + ZrO2 and TCS + CaWO4. TCS + ZrO2, TCS + Nb2O5 and MTAHP had higher mineralized nodule formation than those of the negative control (NC). After 7 days, there was no difference in mRNA expression for ALP, when compared to NC. However, after 14 days there was no overexpressed ALP mRNA, especially TCS + Nb2O5, in relation to the CN. All the materials presented antimicrobial action. Significance: The pure tricalcium silicate associated with ZrO2, CaWO4 or Nb2O5 had appropriate physicochemical properties, antibacterial activity, cytocompatibility and induced mineralization in Saos-2, indicating their use as reparative materials. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:18:00Z 2021-06-25T10:18:00Z 2021-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.dental.2020.11.014 Dental Materials, v. 37, n. 2, p. 311-320, 2021. 0109-5641 http://hdl.handle.net/11449/205591 10.1016/j.dental.2020.11.014 2-s2.0-85097682413 |
url |
http://dx.doi.org/10.1016/j.dental.2020.11.014 http://hdl.handle.net/11449/205591 |
identifier_str_mv |
Dental Materials, v. 37, n. 2, p. 311-320, 2021. 0109-5641 10.1016/j.dental.2020.11.014 2-s2.0-85097682413 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Dental Materials |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
311-320 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129510538739712 |