Aquatic training after joint immobilization in rats promotes adaptations in myotendinous junctions
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ijms22136983 http://hdl.handle.net/11449/221855 |
Resumo: | The myotendinous junction (MTJ) is the muscle-tendon interface and constitutes an integrated mechanical unit to force transmission. Joint immobilization promotes muscle atrophy via disuse, while physical exercise can be used as an adaptative stimulus. In this study, we aimed to investigate the components of the MTJ and their adaptations and the associated elements triggered with aquatic training after joint immobilization. Forty-four male Wistar rats were divided into sedentary (SD), aquatic training (AT), immobilization (IM), and immobilization/aquatic training (IMAT) groups. The samples were processed to measure fiber area, nuclear fractal dimension, MTJ nuclear density, identification of telocytes, sarcomeres, and MTJ perimeter length. In the AT group, the maintenance of ultrastructure and elements in the MTJ region were observed; the IM group presented muscle atrophy effects with reduced MTJ perimeter; the IMAT group demonstrated that aquatic training after joint immobilization promotes benefits in the muscle fiber area and fractal dimension, in the MTJ region shows longer sarcomeres and MTJ perimeter. We identified the presence of telocytes in the MTJ region in all experimental groups. We concluded that aquatic training is an effective rehabilitation method after joint immobilization due to reduced muscle atrophy and regeneration effects on MTJ in rats. |
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Aquatic training after joint immobilization in rats promotes adaptations in myotendinous junctionsAquatic trainingJoint immobilizationMuscle-tendon perimeterNuclear domainSarcomereTelocyteThe myotendinous junction (MTJ) is the muscle-tendon interface and constitutes an integrated mechanical unit to force transmission. Joint immobilization promotes muscle atrophy via disuse, while physical exercise can be used as an adaptative stimulus. In this study, we aimed to investigate the components of the MTJ and their adaptations and the associated elements triggered with aquatic training after joint immobilization. Forty-four male Wistar rats were divided into sedentary (SD), aquatic training (AT), immobilization (IM), and immobilization/aquatic training (IMAT) groups. The samples were processed to measure fiber area, nuclear fractal dimension, MTJ nuclear density, identification of telocytes, sarcomeres, and MTJ perimeter length. In the AT group, the maintenance of ultrastructure and elements in the MTJ region were observed; the IM group presented muscle atrophy effects with reduced MTJ perimeter; the IMAT group demonstrated that aquatic training after joint immobilization promotes benefits in the muscle fiber area and fractal dimension, in the MTJ region shows longer sarcomeres and MTJ perimeter. We identified the presence of telocytes in the MTJ region in all experimental groups. We concluded that aquatic training is an effective rehabilitation method after joint immobilization due to reduced muscle atrophy and regeneration effects on MTJ in rats.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratory of Morphology and Physical Activity (LAMAF) Institute of Biosciences São Paulo State University (UNESP), SPDepartment of Sports Traumatology M51 Bispebjerg and Frederiksberg Hospital IOC Copenhagen Research CenterDepartment of Anatomy Institute of Biomedical Science III University of São Paulo-USP, SPLaboratory of Morphology and Physical Activity (LAMAF) Institute of Biosciences São Paulo State University (UNESP), SPCAPES: 001FAPESP: 2017/12525-1FAPESP: 2018/09199-8Universidade Estadual Paulista (UNESP)IOC Copenhagen Research CenterUniversidade de São Paulo (USP)Rocha, Lara Caetano [UNESP]Barbosa, Gabriela Klein [UNESP]Neto, Jurandyr Pimentel [UNESP]Jacob, Carolina Dos Santos [UNESP]Knudsen, Andreas B.Watanabe, Ii-SeiCiena, Adriano Polican [UNESP]2022-04-28T19:40:57Z2022-04-28T19:40:57Z2021-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ijms22136983International Journal of Molecular Sciences, v. 22, n. 13, 2021.1422-00671661-6596http://hdl.handle.net/11449/22185510.3390/ijms221369832-s2.0-85108830545Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Molecular Sciencesinfo:eu-repo/semantics/openAccess2022-04-28T19:40:57Zoai:repositorio.unesp.br:11449/221855Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:08:36.025279Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Aquatic training after joint immobilization in rats promotes adaptations in myotendinous junctions |
title |
Aquatic training after joint immobilization in rats promotes adaptations in myotendinous junctions |
spellingShingle |
Aquatic training after joint immobilization in rats promotes adaptations in myotendinous junctions Rocha, Lara Caetano [UNESP] Aquatic training Joint immobilization Muscle-tendon perimeter Nuclear domain Sarcomere Telocyte |
title_short |
Aquatic training after joint immobilization in rats promotes adaptations in myotendinous junctions |
title_full |
Aquatic training after joint immobilization in rats promotes adaptations in myotendinous junctions |
title_fullStr |
Aquatic training after joint immobilization in rats promotes adaptations in myotendinous junctions |
title_full_unstemmed |
Aquatic training after joint immobilization in rats promotes adaptations in myotendinous junctions |
title_sort |
Aquatic training after joint immobilization in rats promotes adaptations in myotendinous junctions |
author |
Rocha, Lara Caetano [UNESP] |
author_facet |
Rocha, Lara Caetano [UNESP] Barbosa, Gabriela Klein [UNESP] Neto, Jurandyr Pimentel [UNESP] Jacob, Carolina Dos Santos [UNESP] Knudsen, Andreas B. Watanabe, Ii-Sei Ciena, Adriano Polican [UNESP] |
author_role |
author |
author2 |
Barbosa, Gabriela Klein [UNESP] Neto, Jurandyr Pimentel [UNESP] Jacob, Carolina Dos Santos [UNESP] Knudsen, Andreas B. Watanabe, Ii-Sei Ciena, Adriano Polican [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) IOC Copenhagen Research Center Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Rocha, Lara Caetano [UNESP] Barbosa, Gabriela Klein [UNESP] Neto, Jurandyr Pimentel [UNESP] Jacob, Carolina Dos Santos [UNESP] Knudsen, Andreas B. Watanabe, Ii-Sei Ciena, Adriano Polican [UNESP] |
dc.subject.por.fl_str_mv |
Aquatic training Joint immobilization Muscle-tendon perimeter Nuclear domain Sarcomere Telocyte |
topic |
Aquatic training Joint immobilization Muscle-tendon perimeter Nuclear domain Sarcomere Telocyte |
description |
The myotendinous junction (MTJ) is the muscle-tendon interface and constitutes an integrated mechanical unit to force transmission. Joint immobilization promotes muscle atrophy via disuse, while physical exercise can be used as an adaptative stimulus. In this study, we aimed to investigate the components of the MTJ and their adaptations and the associated elements triggered with aquatic training after joint immobilization. Forty-four male Wistar rats were divided into sedentary (SD), aquatic training (AT), immobilization (IM), and immobilization/aquatic training (IMAT) groups. The samples were processed to measure fiber area, nuclear fractal dimension, MTJ nuclear density, identification of telocytes, sarcomeres, and MTJ perimeter length. In the AT group, the maintenance of ultrastructure and elements in the MTJ region were observed; the IM group presented muscle atrophy effects with reduced MTJ perimeter; the IMAT group demonstrated that aquatic training after joint immobilization promotes benefits in the muscle fiber area and fractal dimension, in the MTJ region shows longer sarcomeres and MTJ perimeter. We identified the presence of telocytes in the MTJ region in all experimental groups. We concluded that aquatic training is an effective rehabilitation method after joint immobilization due to reduced muscle atrophy and regeneration effects on MTJ in rats. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07-01 2022-04-28T19:40:57Z 2022-04-28T19:40:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ijms22136983 International Journal of Molecular Sciences, v. 22, n. 13, 2021. 1422-0067 1661-6596 http://hdl.handle.net/11449/221855 10.3390/ijms22136983 2-s2.0-85108830545 |
url |
http://dx.doi.org/10.3390/ijms22136983 http://hdl.handle.net/11449/221855 |
identifier_str_mv |
International Journal of Molecular Sciences, v. 22, n. 13, 2021. 1422-0067 1661-6596 10.3390/ijms22136983 2-s2.0-85108830545 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Molecular Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129494288957440 |