Highly Effective Fibrin Biopolymer Scaffold for Stem Cells Upgrading Bone Regeneration
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ma13122747 http://hdl.handle.net/11449/197088 |
Resumo: | Fibrin scaffold fits as a provisional platform promoting cell migration and proliferation, angiogenesis, connective tissue formation and growth factors stimulation. We evaluated a unique heterologous fibrin biopolymer as scaffold to mesenchymal stem cells (MSCs) to treat a critical-size bone defect. Femurs of 27 rats were treated with fibrin biopolymer (FBP); FBP + MSCs; and FBP + MSC differentiated in bone lineage (MSC-D). Bone repair was evaluated 03, 21 and 42 days later by radiographic, histological and scanning electron microscopy (SEM) imaging. The FBP + MSC-D association was the most effective treatment, since newly formed Bone was more abundant and early matured in just 21 days. We concluded that FBP is an excellent scaffold for MSCs and also use of differentiated cells should be encouraged in regenerative therapy researches. The FBP ability to maintain viable MSCs at Bone defect site has modified inflammatory environment and accelerating their regeneration. |
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Highly Effective Fibrin Biopolymer Scaffold for Stem Cells Upgrading Bone Regenerationbioproductbiomaterialscaffoldfibrinstem cellFibrin scaffold fits as a provisional platform promoting cell migration and proliferation, angiogenesis, connective tissue formation and growth factors stimulation. We evaluated a unique heterologous fibrin biopolymer as scaffold to mesenchymal stem cells (MSCs) to treat a critical-size bone defect. Femurs of 27 rats were treated with fibrin biopolymer (FBP); FBP + MSCs; and FBP + MSC differentiated in bone lineage (MSC-D). Bone repair was evaluated 03, 21 and 42 days later by radiographic, histological and scanning electron microscopy (SEM) imaging. The FBP + MSC-D association was the most effective treatment, since newly formed Bone was more abundant and early matured in just 21 days. We concluded that FBP is an excellent scaffold for MSCs and also use of differentiated cells should be encouraged in regenerative therapy researches. The FBP ability to maintain viable MSCs at Bone defect site has modified inflammatory environment and accelerating their regeneration.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESP Sao Paulo State Univ, Ctr Study Venoms & Venomous Anim CEVAP, BR-18610307 Botucatu, SP, BrazilUNESP Sao Paulo State Univ, Botucatu Med Sch, BR-18618687 Botucatu, SP, BrazilUNESP Sao Paulo State Univ, Coll Vet Med & Anim Husb FMVZ, BR-18618681 Botucatu, SP, BrazilUNESP Sao Paulo State Univ, Botucatu Biosci Inst, BR-18618689 Botucatu, SP, BrazilUNESP Sao Paulo State Univ, Ctr Study Venoms & Venomous Anim CEVAP, BR-18610307 Botucatu, SP, BrazilUNESP Sao Paulo State Univ, Botucatu Med Sch, BR-18618687 Botucatu, SP, BrazilUNESP Sao Paulo State Univ, Coll Vet Med & Anim Husb FMVZ, BR-18618681 Botucatu, SP, BrazilUNESP Sao Paulo State Univ, Botucatu Biosci Inst, BR-18618689 Botucatu, SP, BrazilCAPES: 23,038.000823/201121CNPq: 303224/20185FAPESP: FAPESP 2013/02004-3MdpiUniversidade Estadual Paulista (Unesp)Creste, Camila Fernanda Zorzella [UNESP]Orsi, Patricia Rodrigues [UNESP]Landim-Alvarenga, Fernanda Cruz [UNESP]Justulin, Luis Antonio [UNESP]Golim, Marjorie de Assis [UNESP]Barraviera, Benedito [UNESP]Ferreira, Rui Seabra [UNESP]2020-12-10T20:05:45Z2020-12-10T20:05:45Z2020-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14http://dx.doi.org/10.3390/ma13122747Materials. Basel: Mdpi, v. 13, n. 12, 14 p., 2020.http://hdl.handle.net/11449/19708810.3390/ma13122747WOS:00055025250000184564903008148330000-0002-2420-2550Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMaterialsinfo:eu-repo/semantics/openAccess2024-04-11T15:28:35Zoai:repositorio.unesp.br:11449/197088Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-11T15:28:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Highly Effective Fibrin Biopolymer Scaffold for Stem Cells Upgrading Bone Regeneration |
title |
Highly Effective Fibrin Biopolymer Scaffold for Stem Cells Upgrading Bone Regeneration |
spellingShingle |
Highly Effective Fibrin Biopolymer Scaffold for Stem Cells Upgrading Bone Regeneration Creste, Camila Fernanda Zorzella [UNESP] bioproduct biomaterial scaffold fibrin stem cell |
title_short |
Highly Effective Fibrin Biopolymer Scaffold for Stem Cells Upgrading Bone Regeneration |
title_full |
Highly Effective Fibrin Biopolymer Scaffold for Stem Cells Upgrading Bone Regeneration |
title_fullStr |
Highly Effective Fibrin Biopolymer Scaffold for Stem Cells Upgrading Bone Regeneration |
title_full_unstemmed |
Highly Effective Fibrin Biopolymer Scaffold for Stem Cells Upgrading Bone Regeneration |
title_sort |
Highly Effective Fibrin Biopolymer Scaffold for Stem Cells Upgrading Bone Regeneration |
author |
Creste, Camila Fernanda Zorzella [UNESP] |
author_facet |
Creste, Camila Fernanda Zorzella [UNESP] Orsi, Patricia Rodrigues [UNESP] Landim-Alvarenga, Fernanda Cruz [UNESP] Justulin, Luis Antonio [UNESP] Golim, Marjorie de Assis [UNESP] Barraviera, Benedito [UNESP] Ferreira, Rui Seabra [UNESP] |
author_role |
author |
author2 |
Orsi, Patricia Rodrigues [UNESP] Landim-Alvarenga, Fernanda Cruz [UNESP] Justulin, Luis Antonio [UNESP] Golim, Marjorie de Assis [UNESP] Barraviera, Benedito [UNESP] Ferreira, Rui Seabra [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Creste, Camila Fernanda Zorzella [UNESP] Orsi, Patricia Rodrigues [UNESP] Landim-Alvarenga, Fernanda Cruz [UNESP] Justulin, Luis Antonio [UNESP] Golim, Marjorie de Assis [UNESP] Barraviera, Benedito [UNESP] Ferreira, Rui Seabra [UNESP] |
dc.subject.por.fl_str_mv |
bioproduct biomaterial scaffold fibrin stem cell |
topic |
bioproduct biomaterial scaffold fibrin stem cell |
description |
Fibrin scaffold fits as a provisional platform promoting cell migration and proliferation, angiogenesis, connective tissue formation and growth factors stimulation. We evaluated a unique heterologous fibrin biopolymer as scaffold to mesenchymal stem cells (MSCs) to treat a critical-size bone defect. Femurs of 27 rats were treated with fibrin biopolymer (FBP); FBP + MSCs; and FBP + MSC differentiated in bone lineage (MSC-D). Bone repair was evaluated 03, 21 and 42 days later by radiographic, histological and scanning electron microscopy (SEM) imaging. The FBP + MSC-D association was the most effective treatment, since newly formed Bone was more abundant and early matured in just 21 days. We concluded that FBP is an excellent scaffold for MSCs and also use of differentiated cells should be encouraged in regenerative therapy researches. The FBP ability to maintain viable MSCs at Bone defect site has modified inflammatory environment and accelerating their regeneration. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T20:05:45Z 2020-12-10T20:05:45Z 2020-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ma13122747 Materials. Basel: Mdpi, v. 13, n. 12, 14 p., 2020. http://hdl.handle.net/11449/197088 10.3390/ma13122747 WOS:000550252500001 8456490300814833 0000-0002-2420-2550 |
url |
http://dx.doi.org/10.3390/ma13122747 http://hdl.handle.net/11449/197088 |
identifier_str_mv |
Materials. Basel: Mdpi, v. 13, n. 12, 14 p., 2020. 10.3390/ma13122747 WOS:000550252500001 8456490300814833 0000-0002-2420-2550 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Materials |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
14 |
dc.publisher.none.fl_str_mv |
Mdpi |
publisher.none.fl_str_mv |
Mdpi |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965756179349504 |