Antibacterial activity of nanoemulsions based on essential oils compounds against species of xanthomonas that cause citrus canker
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.33263/BRIAC122.18351846 http://hdl.handle.net/11449/221955 |
Resumo: | Citrus canker is a bacterial disease that affects citrus crops, caused by microorganisms of the genus Xanthomonas responsible for great damage to citrus production. In this work, the antimicrobial effect of nanoemulsions based on the major compounds of essential oils (EOs) eugenol, thymol, geraniol and menthone were investigated against two strains of Xanthomonas that cause citrus canker (Xac and XauB). Nanoemulsions were produced for each compound using two different stirring speeds (7,000 and 12,000 rpm). All nanoemulsions underwent analysis to characterize particle sizes and stability. Thymol nanoemulsions had the smallest particle sizes (59.8 to 73.9 nm) and the highest stability and also showed the ability to inhibit the development of both strains of bacteria, with minimum inhibitory concentrations (MIC) of 0.03% (v/v), along with eugenol (0.03% (v/v) for Xac and 0.02% (v/v) for XauB) and geraniol nanoemulsions (0.06% (v/v) for both bacteria). Thymol was the compound with the highest minimum bactericidal concentrations (MBC), with values of 0.03% (v/v) for both bacterial strains. Eugenol showed MBC only against XauB, at a concentration of 0.03% (v/v). Geraniol did not show bactericidal activity, suggesting a bacteriostatic action of this compound with the tested microorganisms. In general, the antimicrobial activity of the nanoemulsions was increased with increased agitation speed, particle reduction and greater stability. Therefore, nanoemulsification can be an alternative to applying the antimicrobial activity of natural compounds in the control of citrus canker. |
id |
UNSP_b64ebc035b8dddde7b8c19fe39f6dfae |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/221955 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Antibacterial activity of nanoemulsions based on essential oils compounds against species of xanthomonas that cause citrus cankerCitrus cankerEugenolThymolXanthomonas citri subsp. citriXanthomonas fuscans subsp. aurantifolii type BCitrus canker is a bacterial disease that affects citrus crops, caused by microorganisms of the genus Xanthomonas responsible for great damage to citrus production. In this work, the antimicrobial effect of nanoemulsions based on the major compounds of essential oils (EOs) eugenol, thymol, geraniol and menthone were investigated against two strains of Xanthomonas that cause citrus canker (Xac and XauB). Nanoemulsions were produced for each compound using two different stirring speeds (7,000 and 12,000 rpm). All nanoemulsions underwent analysis to characterize particle sizes and stability. Thymol nanoemulsions had the smallest particle sizes (59.8 to 73.9 nm) and the highest stability and also showed the ability to inhibit the development of both strains of bacteria, with minimum inhibitory concentrations (MIC) of 0.03% (v/v), along with eugenol (0.03% (v/v) for Xac and 0.02% (v/v) for XauB) and geraniol nanoemulsions (0.06% (v/v) for both bacteria). Thymol was the compound with the highest minimum bactericidal concentrations (MBC), with values of 0.03% (v/v) for both bacterial strains. Eugenol showed MBC only against XauB, at a concentration of 0.03% (v/v). Geraniol did not show bactericidal activity, suggesting a bacteriostatic action of this compound with the tested microorganisms. In general, the antimicrobial activity of the nanoemulsions was increased with increased agitation speed, particle reduction and greater stability. Therefore, nanoemulsification can be an alternative to applying the antimicrobial activity of natural compounds in the control of citrus canker.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Biotechnology Federal University of São CarlosSão Paulo State University (UNESP) School of Pharmaceutical SciencesTanquímica Indústria e Comércio LtdaDepartment of Morphology and Patology Federal University of São CarlosBrazilian Agricultural Research Corporation Embrapa InstrumentaçãoSão Paulo State University (UNESP) School of Pharmaceutical SciencesCNPq: # 407956/2016-6CNPq: 101117/2018-3Universidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (UNESP)Tanquímica Indústria e Comércio LtdaEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)da Cruz Silva, Gulhermede Oliveira Filho, Josemar Golçalves [UNESP]Ribeiro, Marilene de Mori Morsellide Souza, Clovis Wesley OliveiraFerreira, Marcos David2022-04-28T19:41:33Z2022-04-28T19:41:33Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1835-1846http://dx.doi.org/10.33263/BRIAC122.18351846Biointerface Research in Applied Chemistry, v. 12, n. 2, p. 1835-1846, 2022.2069-5837http://hdl.handle.net/11449/22195510.33263/BRIAC122.183518462-s2.0-85110100804Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiointerface Research in Applied Chemistryinfo:eu-repo/semantics/openAccess2022-04-28T19:41:33Zoai:repositorio.unesp.br:11449/221955Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:21:30.317719Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antibacterial activity of nanoemulsions based on essential oils compounds against species of xanthomonas that cause citrus canker |
title |
Antibacterial activity of nanoemulsions based on essential oils compounds against species of xanthomonas that cause citrus canker |
spellingShingle |
Antibacterial activity of nanoemulsions based on essential oils compounds against species of xanthomonas that cause citrus canker da Cruz Silva, Gulherme Citrus canker Eugenol Thymol Xanthomonas citri subsp. citri Xanthomonas fuscans subsp. aurantifolii type B |
title_short |
Antibacterial activity of nanoemulsions based on essential oils compounds against species of xanthomonas that cause citrus canker |
title_full |
Antibacterial activity of nanoemulsions based on essential oils compounds against species of xanthomonas that cause citrus canker |
title_fullStr |
Antibacterial activity of nanoemulsions based on essential oils compounds against species of xanthomonas that cause citrus canker |
title_full_unstemmed |
Antibacterial activity of nanoemulsions based on essential oils compounds against species of xanthomonas that cause citrus canker |
title_sort |
Antibacterial activity of nanoemulsions based on essential oils compounds against species of xanthomonas that cause citrus canker |
author |
da Cruz Silva, Gulherme |
author_facet |
da Cruz Silva, Gulherme de Oliveira Filho, Josemar Golçalves [UNESP] Ribeiro, Marilene de Mori Morselli de Souza, Clovis Wesley Oliveira Ferreira, Marcos David |
author_role |
author |
author2 |
de Oliveira Filho, Josemar Golçalves [UNESP] Ribeiro, Marilene de Mori Morselli de Souza, Clovis Wesley Oliveira Ferreira, Marcos David |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Carlos (UFSCar) Universidade Estadual Paulista (UNESP) Tanquímica Indústria e Comércio Ltda Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
da Cruz Silva, Gulherme de Oliveira Filho, Josemar Golçalves [UNESP] Ribeiro, Marilene de Mori Morselli de Souza, Clovis Wesley Oliveira Ferreira, Marcos David |
dc.subject.por.fl_str_mv |
Citrus canker Eugenol Thymol Xanthomonas citri subsp. citri Xanthomonas fuscans subsp. aurantifolii type B |
topic |
Citrus canker Eugenol Thymol Xanthomonas citri subsp. citri Xanthomonas fuscans subsp. aurantifolii type B |
description |
Citrus canker is a bacterial disease that affects citrus crops, caused by microorganisms of the genus Xanthomonas responsible for great damage to citrus production. In this work, the antimicrobial effect of nanoemulsions based on the major compounds of essential oils (EOs) eugenol, thymol, geraniol and menthone were investigated against two strains of Xanthomonas that cause citrus canker (Xac and XauB). Nanoemulsions were produced for each compound using two different stirring speeds (7,000 and 12,000 rpm). All nanoemulsions underwent analysis to characterize particle sizes and stability. Thymol nanoemulsions had the smallest particle sizes (59.8 to 73.9 nm) and the highest stability and also showed the ability to inhibit the development of both strains of bacteria, with minimum inhibitory concentrations (MIC) of 0.03% (v/v), along with eugenol (0.03% (v/v) for Xac and 0.02% (v/v) for XauB) and geraniol nanoemulsions (0.06% (v/v) for both bacteria). Thymol was the compound with the highest minimum bactericidal concentrations (MBC), with values of 0.03% (v/v) for both bacterial strains. Eugenol showed MBC only against XauB, at a concentration of 0.03% (v/v). Geraniol did not show bactericidal activity, suggesting a bacteriostatic action of this compound with the tested microorganisms. In general, the antimicrobial activity of the nanoemulsions was increased with increased agitation speed, particle reduction and greater stability. Therefore, nanoemulsification can be an alternative to applying the antimicrobial activity of natural compounds in the control of citrus canker. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-28T19:41:33Z 2022-04-28T19:41:33Z 2022-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.33263/BRIAC122.18351846 Biointerface Research in Applied Chemistry, v. 12, n. 2, p. 1835-1846, 2022. 2069-5837 http://hdl.handle.net/11449/221955 10.33263/BRIAC122.18351846 2-s2.0-85110100804 |
url |
http://dx.doi.org/10.33263/BRIAC122.18351846 http://hdl.handle.net/11449/221955 |
identifier_str_mv |
Biointerface Research in Applied Chemistry, v. 12, n. 2, p. 1835-1846, 2022. 2069-5837 10.33263/BRIAC122.18351846 2-s2.0-85110100804 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biointerface Research in Applied Chemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1835-1846 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128797609820160 |