OCORRÊNCIA DE AEROMONAS SPP. EM ABATEDOURO BOVINO E SENSIBILIDADE A ANTIMICROBIANOS
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1808-1657v77p1952010 http://hdl.handle.net/11449/211992 |
Resumo: | The genus Aeromonas comprises important human pathogens, gastroenteritis being the most common type of infection attributed to these bacteria. Considering their importance as foodborne pathogens, the occurrence of Aeromonas spp. was studied on bovine carcasses and the environment of a slaughterhouse in São Paulo State, Brazil. A total of 285 samples were collected from 19 points. Direct counting was carried out by seeding on selective medium, with biochemical characterization of the species after selective enrichment and antimicrobial sensitivity testing. The direct count permitted bacteria quantification only in 12 samples ranging from 0.5 to 9.2 x 100 CFU/ cm2, of which 5 were from environment, ranging from 1.0 x 100 to 3.0 x 100 CFU/plate. However, after selective enrichment,Aeromonas spp. was isolated from 38 samples, which when added to the environmental samples not subjected to the enrichment provided 62 isolates for analysis. Biochemical characterization allowed for the verification of the occurrence of 59 isolates of A. caviae, 1 ofA. sobria, 1 ofA. trota and 1 ofA. schubertii. Antimicrobial testing revealed that all isolates were resistant to ampicillin and cephalotin, while the results for the other antimicrobials were variable. Resistance of all isolates to some antimicrobials indicates that these must be used carefully to avoid early development of multiresistantAeromonas spp. Also, the greatest prevalence of A. caviae must be considered relevant because it is one of the causative species of human gastroenteritis. |
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OCORRÊNCIA DE AEROMONAS SPP. EM ABATEDOURO BOVINO E SENSIBILIDADE A ANTIMICROBIANOSOCCURRENCE OF AEROMONAS SPP. IN BOVINE ABATTOIR AND ANTIMICROBIAL SENSITIVITYAeromonas sppantimicrobial sensitivitybovine abattoirAeromonas sppsensibilidade antimicrobianaabatedouro bovinoThe genus Aeromonas comprises important human pathogens, gastroenteritis being the most common type of infection attributed to these bacteria. Considering their importance as foodborne pathogens, the occurrence of Aeromonas spp. was studied on bovine carcasses and the environment of a slaughterhouse in São Paulo State, Brazil. A total of 285 samples were collected from 19 points. Direct counting was carried out by seeding on selective medium, with biochemical characterization of the species after selective enrichment and antimicrobial sensitivity testing. The direct count permitted bacteria quantification only in 12 samples ranging from 0.5 to 9.2 x 100 CFU/ cm2, of which 5 were from environment, ranging from 1.0 x 100 to 3.0 x 100 CFU/plate. However, after selective enrichment,Aeromonas spp. was isolated from 38 samples, which when added to the environmental samples not subjected to the enrichment provided 62 isolates for analysis. Biochemical characterization allowed for the verification of the occurrence of 59 isolates of A. caviae, 1 ofA. sobria, 1 ofA. trota and 1 ofA. schubertii. Antimicrobial testing revealed that all isolates were resistant to ampicillin and cephalotin, while the results for the other antimicrobials were variable. Resistance of all isolates to some antimicrobials indicates that these must be used carefully to avoid early development of multiresistantAeromonas spp. Also, the greatest prevalence of A. caviae must be considered relevant because it is one of the causative species of human gastroenteritis.O gênero Aeromonas compreende espécies consideradas importantes patógenos para os seres humanos, sendo as gastroenterites as infecções mais comumente atribuídas a estas bactérias. Tendo em vista sua importância como patógeno de origem alimentar, a ocorrência deAeromonas spp. foi estudada em carcaças bovinas e ambiente do abatedouro em uma indústria do Estado de São Paulo. Foram colhidas 285 amostras de 19 locais. Foi realizada a contagem direta por semeadura em meio seletivo, caracterização bioquímica das espécies após enriquecimento seletivo e teste de sensibilidade a antimicrobianos. A contagem direta permitiu a quantificação dessas bactérias em apenas 12 amostras, variando de 0,5 a 9,2 x 100 UFC/cm2, sendo cinco delas de ambiente, com populações que variaram de 1,0 x 100 a 3,0 x 100 UFC/placa. Entretanto, após o enriquecimento seletivo, Aeromonas spp. foram isoladas de 38 amostras que, somadas às amostras de ambiente não submetidas ao enriquecimento, forneceram 62 isolados para análise. A caracterização bioquímica das espécies permitiu verificar a ocorrência de 59 isolados de A. caviae, um de A. sobria, um de A. trota e um de A. schubertii. O teste com antimicrobianos revelou resistência de todos os isolados pela ampicilina e cefalotina, enquanto, para os demais antimicrobianos, esta foi variável. A resistência da totalidade dos isolados a determinados antimicrobianos indica que estes devem ser utilizados criteriosamente com a finalidade de evitar o surgimento precoce de cepas deAeromonas spp. multirresistentes. Ainda, a maior prevalência deA. caviae deve ser considerada relevante, pois trata-se de uma das espécies causadoras de gastroenterite em humanos.Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Ciências Agrárias e VeterináriasUniversidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Ciências Agrárias e VeterináriasInstituto BiológicoUniversidade Estadual Paulista (Unesp)Martineli, T.m. [UNESP]Rossi, O.d [UNESP]Cereser, N.d. [UNESP]Cardozo, M.v. [UNESP]Kamimura, B.a. [UNESP]Nespolo, N.m. [UNESP]Pinto, F.r. [UNESP]2021-07-14T10:32:42Z2021-07-14T10:32:42Z2020-11-16info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article195-202application/pdfhttp://dx.doi.org/10.1590/1808-1657v77p1952010Arquivos do Instituto Biológico. Instituto Biológico, v. 77, n. 2, p. 195-202, 2020.0020-36531808-1657http://hdl.handle.net/11449/21199210.1590/1808-1657v77p1952010S1808-16572010000200195S1808-16572010000200195.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporArquivos do Instituto Biológicoinfo:eu-repo/semantics/openAccess2023-12-08T06:25:06Zoai:repositorio.unesp.br:11449/211992Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:48:51.308742Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
OCORRÊNCIA DE AEROMONAS SPP. EM ABATEDOURO BOVINO E SENSIBILIDADE A ANTIMICROBIANOS OCCURRENCE OF AEROMONAS SPP. IN BOVINE ABATTOIR AND ANTIMICROBIAL SENSITIVITY |
title |
OCORRÊNCIA DE AEROMONAS SPP. EM ABATEDOURO BOVINO E SENSIBILIDADE A ANTIMICROBIANOS |
spellingShingle |
OCORRÊNCIA DE AEROMONAS SPP. EM ABATEDOURO BOVINO E SENSIBILIDADE A ANTIMICROBIANOS Martineli, T.m. [UNESP] Aeromonas spp antimicrobial sensitivity bovine abattoir Aeromonas spp sensibilidade antimicrobiana abatedouro bovino |
title_short |
OCORRÊNCIA DE AEROMONAS SPP. EM ABATEDOURO BOVINO E SENSIBILIDADE A ANTIMICROBIANOS |
title_full |
OCORRÊNCIA DE AEROMONAS SPP. EM ABATEDOURO BOVINO E SENSIBILIDADE A ANTIMICROBIANOS |
title_fullStr |
OCORRÊNCIA DE AEROMONAS SPP. EM ABATEDOURO BOVINO E SENSIBILIDADE A ANTIMICROBIANOS |
title_full_unstemmed |
OCORRÊNCIA DE AEROMONAS SPP. EM ABATEDOURO BOVINO E SENSIBILIDADE A ANTIMICROBIANOS |
title_sort |
OCORRÊNCIA DE AEROMONAS SPP. EM ABATEDOURO BOVINO E SENSIBILIDADE A ANTIMICROBIANOS |
author |
Martineli, T.m. [UNESP] |
author_facet |
Martineli, T.m. [UNESP] Rossi, O.d [UNESP] Cereser, N.d. [UNESP] Cardozo, M.v. [UNESP] Kamimura, B.a. [UNESP] Nespolo, N.m. [UNESP] Pinto, F.r. [UNESP] |
author_role |
author |
author2 |
Rossi, O.d [UNESP] Cereser, N.d. [UNESP] Cardozo, M.v. [UNESP] Kamimura, B.a. [UNESP] Nespolo, N.m. [UNESP] Pinto, F.r. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Martineli, T.m. [UNESP] Rossi, O.d [UNESP] Cereser, N.d. [UNESP] Cardozo, M.v. [UNESP] Kamimura, B.a. [UNESP] Nespolo, N.m. [UNESP] Pinto, F.r. [UNESP] |
dc.subject.por.fl_str_mv |
Aeromonas spp antimicrobial sensitivity bovine abattoir Aeromonas spp sensibilidade antimicrobiana abatedouro bovino |
topic |
Aeromonas spp antimicrobial sensitivity bovine abattoir Aeromonas spp sensibilidade antimicrobiana abatedouro bovino |
description |
The genus Aeromonas comprises important human pathogens, gastroenteritis being the most common type of infection attributed to these bacteria. Considering their importance as foodborne pathogens, the occurrence of Aeromonas spp. was studied on bovine carcasses and the environment of a slaughterhouse in São Paulo State, Brazil. A total of 285 samples were collected from 19 points. Direct counting was carried out by seeding on selective medium, with biochemical characterization of the species after selective enrichment and antimicrobial sensitivity testing. The direct count permitted bacteria quantification only in 12 samples ranging from 0.5 to 9.2 x 100 CFU/ cm2, of which 5 were from environment, ranging from 1.0 x 100 to 3.0 x 100 CFU/plate. However, after selective enrichment,Aeromonas spp. was isolated from 38 samples, which when added to the environmental samples not subjected to the enrichment provided 62 isolates for analysis. Biochemical characterization allowed for the verification of the occurrence of 59 isolates of A. caviae, 1 ofA. sobria, 1 ofA. trota and 1 ofA. schubertii. Antimicrobial testing revealed that all isolates were resistant to ampicillin and cephalotin, while the results for the other antimicrobials were variable. Resistance of all isolates to some antimicrobials indicates that these must be used carefully to avoid early development of multiresistantAeromonas spp. Also, the greatest prevalence of A. caviae must be considered relevant because it is one of the causative species of human gastroenteritis. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-16 2021-07-14T10:32:42Z 2021-07-14T10:32:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1808-1657v77p1952010 Arquivos do Instituto Biológico. Instituto Biológico, v. 77, n. 2, p. 195-202, 2020. 0020-3653 1808-1657 http://hdl.handle.net/11449/211992 10.1590/1808-1657v77p1952010 S1808-16572010000200195 S1808-16572010000200195.pdf |
url |
http://dx.doi.org/10.1590/1808-1657v77p1952010 http://hdl.handle.net/11449/211992 |
identifier_str_mv |
Arquivos do Instituto Biológico. Instituto Biológico, v. 77, n. 2, p. 195-202, 2020. 0020-3653 1808-1657 10.1590/1808-1657v77p1952010 S1808-16572010000200195 S1808-16572010000200195.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Arquivos do Instituto Biológico |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
195-202 application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Biológico |
publisher.none.fl_str_mv |
Instituto Biológico |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129121679572992 |