OCORRÊNCIA DE AEROMONAS SPP. EM ABATEDOURO BOVINO E SENSIBILIDADE A ANTIMICROBIANOS

Detalhes bibliográficos
Autor(a) principal: Martineli, T.m. [UNESP]
Data de Publicação: 2020
Outros Autores: Rossi, O.d [UNESP], Cereser, N.d. [UNESP], Cardozo, M.v. [UNESP], Kamimura, B.a. [UNESP], Nespolo, N.m. [UNESP], Pinto, F.r. [UNESP]
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1808-1657v77p1952010
http://hdl.handle.net/11449/211992
Resumo: The genus Aeromonas comprises important human pathogens, gastroenteritis being the most common type of infection attributed to these bacteria. Considering their importance as foodborne pathogens, the occurrence of Aeromonas spp. was studied on bovine carcasses and the environment of a slaughterhouse in São Paulo State, Brazil. A total of 285 samples were collected from 19 points. Direct counting was carried out by seeding on selective medium, with biochemical characterization of the species after selective enrichment and antimicrobial sensitivity testing. The direct count permitted bacteria quantification only in 12 samples ranging from 0.5 to 9.2 x 100 CFU/ cm2, of which 5 were from environment, ranging from 1.0 x 100 to 3.0 x 100 CFU/plate. However, after selective enrichment,Aeromonas spp. was isolated from 38 samples, which when added to the environmental samples not subjected to the enrichment provided 62 isolates for analysis. Biochemical characterization allowed for the verification of the occurrence of 59 isolates of A. caviae, 1 ofA. sobria, 1 ofA. trota and 1 ofA. schubertii. Antimicrobial testing revealed that all isolates were resistant to ampicillin and cephalotin, while the results for the other antimicrobials were variable. Resistance of all isolates to some antimicrobials indicates that these must be used carefully to avoid early development of multiresistantAeromonas spp. Also, the greatest prevalence of A. caviae must be considered relevant because it is one of the causative species of human gastroenteritis.
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spelling OCORRÊNCIA DE AEROMONAS SPP. EM ABATEDOURO BOVINO E SENSIBILIDADE A ANTIMICROBIANOSOCCURRENCE OF AEROMONAS SPP. IN BOVINE ABATTOIR AND ANTIMICROBIAL SENSITIVITYAeromonas sppantimicrobial sensitivitybovine abattoirAeromonas sppsensibilidade antimicrobianaabatedouro bovinoThe genus Aeromonas comprises important human pathogens, gastroenteritis being the most common type of infection attributed to these bacteria. Considering their importance as foodborne pathogens, the occurrence of Aeromonas spp. was studied on bovine carcasses and the environment of a slaughterhouse in São Paulo State, Brazil. A total of 285 samples were collected from 19 points. Direct counting was carried out by seeding on selective medium, with biochemical characterization of the species after selective enrichment and antimicrobial sensitivity testing. The direct count permitted bacteria quantification only in 12 samples ranging from 0.5 to 9.2 x 100 CFU/ cm2, of which 5 were from environment, ranging from 1.0 x 100 to 3.0 x 100 CFU/plate. However, after selective enrichment,Aeromonas spp. was isolated from 38 samples, which when added to the environmental samples not subjected to the enrichment provided 62 isolates for analysis. Biochemical characterization allowed for the verification of the occurrence of 59 isolates of A. caviae, 1 ofA. sobria, 1 ofA. trota and 1 ofA. schubertii. Antimicrobial testing revealed that all isolates were resistant to ampicillin and cephalotin, while the results for the other antimicrobials were variable. Resistance of all isolates to some antimicrobials indicates that these must be used carefully to avoid early development of multiresistantAeromonas spp. Also, the greatest prevalence of A. caviae must be considered relevant because it is one of the causative species of human gastroenteritis.O gênero Aeromonas compreende espécies consideradas importantes patógenos para os seres humanos, sendo as gastroenterites as infecções mais comumente atribuídas a estas bactérias. Tendo em vista sua importância como patógeno de origem alimentar, a ocorrência deAeromonas spp. foi estudada em carcaças bovinas e ambiente do abatedouro em uma indústria do Estado de São Paulo. Foram colhidas 285 amostras de 19 locais. Foi realizada a contagem direta por semeadura em meio seletivo, caracterização bioquímica das espécies após enriquecimento seletivo e teste de sensibilidade a antimicrobianos. A contagem direta permitiu a quantificação dessas bactérias em apenas 12 amostras, variando de 0,5 a 9,2 x 100 UFC/cm2, sendo cinco delas de ambiente, com populações que variaram de 1,0 x 100 a 3,0 x 100 UFC/placa. Entretanto, após o enriquecimento seletivo, Aeromonas spp. foram isoladas de 38 amostras que, somadas às amostras de ambiente não submetidas ao enriquecimento, forneceram 62 isolados para análise. A caracterização bioquímica das espécies permitiu verificar a ocorrência de 59 isolados de A. caviae, um de A. sobria, um de A. trota e um de A. schubertii. O teste com antimicrobianos revelou resistência de todos os isolados pela ampicilina e cefalotina, enquanto, para os demais antimicrobianos, esta foi variável. A resistência da totalidade dos isolados a determinados antimicrobianos indica que estes devem ser utilizados criteriosamente com a finalidade de evitar o surgimento precoce de cepas deAeromonas spp. multirresistentes. Ainda, a maior prevalência deA. caviae deve ser considerada relevante, pois trata-se de uma das espécies causadoras de gastroenterite em humanos.Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Ciências Agrárias e VeterináriasUniversidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Ciências Agrárias e VeterináriasInstituto BiológicoUniversidade Estadual Paulista (Unesp)Martineli, T.m. [UNESP]Rossi, O.d [UNESP]Cereser, N.d. [UNESP]Cardozo, M.v. [UNESP]Kamimura, B.a. [UNESP]Nespolo, N.m. [UNESP]Pinto, F.r. [UNESP]2021-07-14T10:32:42Z2021-07-14T10:32:42Z2020-11-16info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article195-202application/pdfhttp://dx.doi.org/10.1590/1808-1657v77p1952010Arquivos do Instituto Biológico. Instituto Biológico, v. 77, n. 2, p. 195-202, 2020.0020-36531808-1657http://hdl.handle.net/11449/21199210.1590/1808-1657v77p1952010S1808-16572010000200195S1808-16572010000200195.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporArquivos do Instituto Biológicoinfo:eu-repo/semantics/openAccess2023-12-08T06:25:06Zoai:repositorio.unesp.br:11449/211992Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-08T06:25:06Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv OCORRÊNCIA DE AEROMONAS SPP. EM ABATEDOURO BOVINO E SENSIBILIDADE A ANTIMICROBIANOS
OCCURRENCE OF AEROMONAS SPP. IN BOVINE ABATTOIR AND ANTIMICROBIAL SENSITIVITY
title OCORRÊNCIA DE AEROMONAS SPP. EM ABATEDOURO BOVINO E SENSIBILIDADE A ANTIMICROBIANOS
spellingShingle OCORRÊNCIA DE AEROMONAS SPP. EM ABATEDOURO BOVINO E SENSIBILIDADE A ANTIMICROBIANOS
Martineli, T.m. [UNESP]
Aeromonas spp
antimicrobial sensitivity
bovine abattoir
Aeromonas spp
sensibilidade antimicrobiana
abatedouro bovino
title_short OCORRÊNCIA DE AEROMONAS SPP. EM ABATEDOURO BOVINO E SENSIBILIDADE A ANTIMICROBIANOS
title_full OCORRÊNCIA DE AEROMONAS SPP. EM ABATEDOURO BOVINO E SENSIBILIDADE A ANTIMICROBIANOS
title_fullStr OCORRÊNCIA DE AEROMONAS SPP. EM ABATEDOURO BOVINO E SENSIBILIDADE A ANTIMICROBIANOS
title_full_unstemmed OCORRÊNCIA DE AEROMONAS SPP. EM ABATEDOURO BOVINO E SENSIBILIDADE A ANTIMICROBIANOS
title_sort OCORRÊNCIA DE AEROMONAS SPP. EM ABATEDOURO BOVINO E SENSIBILIDADE A ANTIMICROBIANOS
author Martineli, T.m. [UNESP]
author_facet Martineli, T.m. [UNESP]
Rossi, O.d [UNESP]
Cereser, N.d. [UNESP]
Cardozo, M.v. [UNESP]
Kamimura, B.a. [UNESP]
Nespolo, N.m. [UNESP]
Pinto, F.r. [UNESP]
author_role author
author2 Rossi, O.d [UNESP]
Cereser, N.d. [UNESP]
Cardozo, M.v. [UNESP]
Kamimura, B.a. [UNESP]
Nespolo, N.m. [UNESP]
Pinto, F.r. [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Martineli, T.m. [UNESP]
Rossi, O.d [UNESP]
Cereser, N.d. [UNESP]
Cardozo, M.v. [UNESP]
Kamimura, B.a. [UNESP]
Nespolo, N.m. [UNESP]
Pinto, F.r. [UNESP]
dc.subject.por.fl_str_mv Aeromonas spp
antimicrobial sensitivity
bovine abattoir
Aeromonas spp
sensibilidade antimicrobiana
abatedouro bovino
topic Aeromonas spp
antimicrobial sensitivity
bovine abattoir
Aeromonas spp
sensibilidade antimicrobiana
abatedouro bovino
description The genus Aeromonas comprises important human pathogens, gastroenteritis being the most common type of infection attributed to these bacteria. Considering their importance as foodborne pathogens, the occurrence of Aeromonas spp. was studied on bovine carcasses and the environment of a slaughterhouse in São Paulo State, Brazil. A total of 285 samples were collected from 19 points. Direct counting was carried out by seeding on selective medium, with biochemical characterization of the species after selective enrichment and antimicrobial sensitivity testing. The direct count permitted bacteria quantification only in 12 samples ranging from 0.5 to 9.2 x 100 CFU/ cm2, of which 5 were from environment, ranging from 1.0 x 100 to 3.0 x 100 CFU/plate. However, after selective enrichment,Aeromonas spp. was isolated from 38 samples, which when added to the environmental samples not subjected to the enrichment provided 62 isolates for analysis. Biochemical characterization allowed for the verification of the occurrence of 59 isolates of A. caviae, 1 ofA. sobria, 1 ofA. trota and 1 ofA. schubertii. Antimicrobial testing revealed that all isolates were resistant to ampicillin and cephalotin, while the results for the other antimicrobials were variable. Resistance of all isolates to some antimicrobials indicates that these must be used carefully to avoid early development of multiresistantAeromonas spp. Also, the greatest prevalence of A. caviae must be considered relevant because it is one of the causative species of human gastroenteritis.
publishDate 2020
dc.date.none.fl_str_mv 2020-11-16
2021-07-14T10:32:42Z
2021-07-14T10:32:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1808-1657v77p1952010
Arquivos do Instituto Biológico. Instituto Biológico, v. 77, n. 2, p. 195-202, 2020.
0020-3653
1808-1657
http://hdl.handle.net/11449/211992
10.1590/1808-1657v77p1952010
S1808-16572010000200195
S1808-16572010000200195.pdf
url http://dx.doi.org/10.1590/1808-1657v77p1952010
http://hdl.handle.net/11449/211992
identifier_str_mv Arquivos do Instituto Biológico. Instituto Biológico, v. 77, n. 2, p. 195-202, 2020.
0020-3653
1808-1657
10.1590/1808-1657v77p1952010
S1808-16572010000200195
S1808-16572010000200195.pdf
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Arquivos do Instituto Biológico
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 195-202
application/pdf
dc.publisher.none.fl_str_mv Instituto Biológico
publisher.none.fl_str_mv Instituto Biológico
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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