Production and evaluation of leukocyte-and thrombocyte-rich fibrin membranes in birds

• Autor(a) principal: Fernandes, Laís L. [UNESP]
• Data de Publicação: 2019
• Outros Autores: Rahal, Sheila C. [UNESP], Fabro, Alexandre T., Batah, Sabrina S., Hippólito, Alicia G. [UNESP], Bisca, Jacqueline M. [UNESP], Hirot, Inajara N. [UNESP], Teixeira, Carlos R. [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1556/004.2019.030; http://hdl.handle.net/11449/189395
• topic: Avian; Fibrin membrane; Histology; L-TRF; Protocol

The aim of this study was the preparation and histological evaluation of Leukocyte- and Thrombocyte-Rich Fibrin (L-TRF) membranes obtained from the blood of four bird species. Forty adult healthy birds were divided into four groups of equal size: G1 – macaws, G2 – domestic chickens, G3 – parrots, G4 – toco toucans. A total of 0.5 mL of blood was collected from each bird, put into a glass tube without anticoagulant and centrifuged at 3000 rpm for 10 min. L-TRF membranes produced after compression of the clot were processed for histological analysis. The ratio of thrombocytes/area was not significantly different among Groups G2, G3 and G4, but a significant difference was found between Groups G1 and G2 with the highest thrombocyte concentration/area in G1. The groups did not differ statistically in the number of leukocytes/area. The fibrin-to-cells ratio did not vary statistically among Groups G1, G2 and G3, but this ratio was significantly higher in Group G4 than in the other groups. The thrombocyte-to-leukocyte ratio was the highest in Group G1, but it did not differ among Groups G2, G3 and G4. In conclusion, the centrifugation protocol allowed the production of L-TRF membranes in the four bird species studied. Histologically, cell ratios were analogous in domestic chickens and parrots, and macaws had the highest ratio of thrombocytes.