A5 noradrenergic neurons and breathing control in neonate rats

Detalhes bibliográficos
Autor(a) principal: Taxini, Camila L. [UNESP]
Data de Publicação: 2021
Outros Autores: Marques, Danuzia A., Bícego, Kênia C. [UNESP], Gargaglioni, Luciane H. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00424-021-02550-1
http://hdl.handle.net/11449/207608
Resumo: The pontine A5 noradrenergic group contributes to the maturation of the respiratory system before birth in rats. These neurons are connected to the neural network responsible for respiratory rhythmogenesis. In the present study, we investigated the participation of A5 noradrenergic neurons in neonates (P7–8 and P14–15) in the control of ventilation during hypoxia and hypercapnia in in vivo experiments using conjugated saporin anti-dopamine beta-hydroxylase (DβH-SAP) to specifically ablate noradrenergic neurons. Thus, DβH-SAP (420 ng/μL) or saporin (SAP, control) was injected into the A5 region of neonatal male Wistar rats. Hypoxia reduced respiratory variability in control animals; however, A5 lesion prevented this effect in P7–8 rats. Our data suggest that noradrenergic neurons of the A5 region in neonate rats do not participate in the control of ventilation under baseline and hypercapnic conditions, but exert an inhibitory modulation on breathing variability under hypoxic challenge in early life (P7–8).
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spelling A5 noradrenergic neurons and breathing control in neonate ratsBrainstemCatecholaminergic neuronsDevelopmentHypercapniaHypoxiaThe pontine A5 noradrenergic group contributes to the maturation of the respiratory system before birth in rats. These neurons are connected to the neural network responsible for respiratory rhythmogenesis. In the present study, we investigated the participation of A5 noradrenergic neurons in neonates (P7–8 and P14–15) in the control of ventilation during hypoxia and hypercapnia in in vivo experiments using conjugated saporin anti-dopamine beta-hydroxylase (DβH-SAP) to specifically ablate noradrenergic neurons. Thus, DβH-SAP (420 ng/μL) or saporin (SAP, control) was injected into the A5 region of neonatal male Wistar rats. Hypoxia reduced respiratory variability in control animals; however, A5 lesion prevented this effect in P7–8 rats. Our data suggest that noradrenergic neurons of the A5 region in neonate rats do not participate in the control of ventilation under baseline and hypercapnic conditions, but exert an inhibitory modulation on breathing variability under hypoxic challenge in early life (P7–8).Department of Morphology and Animal Physiology – FCAV São Paulo State University (UNESP), Via de acesso Paulo Donato Castellane s/nDepartment of Pediatrics Centre de Recherche de L’Institut Universitaire de Cardiologie Et de Pneumologie de Québec Université LavalDepartment of Morphology and Animal Physiology – FCAV São Paulo State University (UNESP), Via de acesso Paulo Donato Castellane s/nUniversidade Estadual Paulista (Unesp)Université LavalTaxini, Camila L. [UNESP]Marques, Danuzia A.Bícego, Kênia C. [UNESP]Gargaglioni, Luciane H. [UNESP]2021-06-25T10:58:04Z2021-06-25T10:58:04Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s00424-021-02550-1Pflugers Archiv European Journal of Physiology.1432-20130031-6768http://hdl.handle.net/11449/20760810.1007/s00424-021-02550-12-s2.0-85104292353Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPflugers Archiv European Journal of Physiologyinfo:eu-repo/semantics/openAccess2024-06-06T18:42:13Zoai:repositorio.unesp.br:11449/207608Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:29:05.180272Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A5 noradrenergic neurons and breathing control in neonate rats
title A5 noradrenergic neurons and breathing control in neonate rats
spellingShingle A5 noradrenergic neurons and breathing control in neonate rats
Taxini, Camila L. [UNESP]
Brainstem
Catecholaminergic neurons
Development
Hypercapnia
Hypoxia
title_short A5 noradrenergic neurons and breathing control in neonate rats
title_full A5 noradrenergic neurons and breathing control in neonate rats
title_fullStr A5 noradrenergic neurons and breathing control in neonate rats
title_full_unstemmed A5 noradrenergic neurons and breathing control in neonate rats
title_sort A5 noradrenergic neurons and breathing control in neonate rats
author Taxini, Camila L. [UNESP]
author_facet Taxini, Camila L. [UNESP]
Marques, Danuzia A.
Bícego, Kênia C. [UNESP]
Gargaglioni, Luciane H. [UNESP]
author_role author
author2 Marques, Danuzia A.
Bícego, Kênia C. [UNESP]
Gargaglioni, Luciane H. [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Université Laval
dc.contributor.author.fl_str_mv Taxini, Camila L. [UNESP]
Marques, Danuzia A.
Bícego, Kênia C. [UNESP]
Gargaglioni, Luciane H. [UNESP]
dc.subject.por.fl_str_mv Brainstem
Catecholaminergic neurons
Development
Hypercapnia
Hypoxia
topic Brainstem
Catecholaminergic neurons
Development
Hypercapnia
Hypoxia
description The pontine A5 noradrenergic group contributes to the maturation of the respiratory system before birth in rats. These neurons are connected to the neural network responsible for respiratory rhythmogenesis. In the present study, we investigated the participation of A5 noradrenergic neurons in neonates (P7–8 and P14–15) in the control of ventilation during hypoxia and hypercapnia in in vivo experiments using conjugated saporin anti-dopamine beta-hydroxylase (DβH-SAP) to specifically ablate noradrenergic neurons. Thus, DβH-SAP (420 ng/μL) or saporin (SAP, control) was injected into the A5 region of neonatal male Wistar rats. Hypoxia reduced respiratory variability in control animals; however, A5 lesion prevented this effect in P7–8 rats. Our data suggest that noradrenergic neurons of the A5 region in neonate rats do not participate in the control of ventilation under baseline and hypercapnic conditions, but exert an inhibitory modulation on breathing variability under hypoxic challenge in early life (P7–8).
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:58:04Z
2021-06-25T10:58:04Z
2021-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00424-021-02550-1
Pflugers Archiv European Journal of Physiology.
1432-2013
0031-6768
http://hdl.handle.net/11449/207608
10.1007/s00424-021-02550-1
2-s2.0-85104292353
url http://dx.doi.org/10.1007/s00424-021-02550-1
http://hdl.handle.net/11449/207608
identifier_str_mv Pflugers Archiv European Journal of Physiology.
1432-2013
0031-6768
10.1007/s00424-021-02550-1
2-s2.0-85104292353
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pflugers Archiv European Journal of Physiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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