Mediadores da via intrínseca da morte celular programada relacionados à infecção in vitro pelo herpesvirus bovino tipo 5

• Autor(a) principal: Garcia, Andréa Fontes [UNESP]
• Data de Publicação: 2013
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/121912
• topic: Apoptose; Viroses; Encefalite; Imunidade celular; Apoptosis

Bovine herpesvirus 5 (BoHV-5) is α-herpesvirus responsible for neurological disease in young cattle and also occasionally incriminated in reproductive disorders.In spite of many studies describing the apoptotic pathways induced by viruses belonging to the family Herpesviridae, scare information about intrinsic programmed cell death pathway in host-BoHV-5 interactions is currently available. BoHV-5 was able to replicate and to produce cytopathology characterized by cellular swelling and cell fusion on both mesenchymal and epithelial cell lines. Viral antigens were detected in infected cells by immunofluorescence assay at 48 to 96 h post-infection (p.i.). According to sequential p.i observation, at 48 to 72 h p.i. higher fluorescent signals were visible for anti-apoptotic BCl-2 antigens in comparison to Bax, considered a proapoptotic protein. Infected cells revealed an increase of BCl-2 phenotype population from 48 to 96 h p.i. by flow cytometric analysis. At 48 to 96 h p.i. Bax mRNA was not expressed among of any infected cell monolayers. In contrast, BCl-2 mRNA was quantified Log10 1,6 x 102 cDNA copies at all p.i. for both cells. BoHV-5 replication seems to modulate BCl-2 expression and respective gene transcription in order to enhance production of progeny virus