Differential roles of hippocampal nNOS and iNOS in the control of baroreflex function in conscious rats
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.brainres.2018.12.044 http://hdl.handle.net/11449/187227 |
Resumo: | The baroreflex is a prominent moment-to-moment mechanism regulating the blood pressure. The hippocampus is a limbic structure in which has been pointed out as part of central network regulating baroreflex. However, the local neurochemical mechanisms involved in control of baroreflex function are not completely understood. Thus, this study aimed to investigate the involvement of nitrergic neurotransmission present in the dorsal hippocampus in baroreflex control of heart rate in conscious rats. For this, we evaluated the effect of bilateral microinjection into the dorsal hippocampus of either the nitric oxide (NO) scavenger carboxy-PTIO, the selective neuronal nitric oxide synthase (nNOS) inhibitor Nω-Propyl-L-arginine (NPLA) or the selective inducible nitric oxide synthase (iNOS) inhibitor 1400 W in bradycardia evoked by blood pressure increases in response to intravenous infusion of phenylephrine, and tachycardia caused by blood pressure decreases evoked by intravenous infusion of sodium nitroprusside. Bilateral microinjection of carboxy-PTIO into the dorsal hippocampus decreased the baroreflex tachycardic response without affecting the reflex bradycardia. Hippocampus treatment with NPLA increased the baroreflex bradycardia gain without affecting the reflex tachycardia. Bilateral hippocampal treatment with 1400 W decreased the reflex tachycardia and increased the baroreflex bradycardic response. Overall, these findings provide evidence that hippocampal nitrergic mechanisms acting in a NOS isoform-specific manner plays a prominent role in control of baroreflex function. Indeed, the results indicate that nNOS and iNOS exerts an inhibitory influence on reflex bradycardia, whereas iNOS mediates the reflex tachycardia. |
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Differential roles of hippocampal nNOS and iNOS in the control of baroreflex function in conscious rats1400 WArterial pressureCardiovascularHeart rateN-Propyl-L-arginineNOThe baroreflex is a prominent moment-to-moment mechanism regulating the blood pressure. The hippocampus is a limbic structure in which has been pointed out as part of central network regulating baroreflex. However, the local neurochemical mechanisms involved in control of baroreflex function are not completely understood. Thus, this study aimed to investigate the involvement of nitrergic neurotransmission present in the dorsal hippocampus in baroreflex control of heart rate in conscious rats. For this, we evaluated the effect of bilateral microinjection into the dorsal hippocampus of either the nitric oxide (NO) scavenger carboxy-PTIO, the selective neuronal nitric oxide synthase (nNOS) inhibitor Nω-Propyl-L-arginine (NPLA) or the selective inducible nitric oxide synthase (iNOS) inhibitor 1400 W in bradycardia evoked by blood pressure increases in response to intravenous infusion of phenylephrine, and tachycardia caused by blood pressure decreases evoked by intravenous infusion of sodium nitroprusside. Bilateral microinjection of carboxy-PTIO into the dorsal hippocampus decreased the baroreflex tachycardic response without affecting the reflex bradycardia. Hippocampus treatment with NPLA increased the baroreflex bradycardia gain without affecting the reflex tachycardia. Bilateral hippocampal treatment with 1400 W decreased the reflex tachycardia and increased the baroreflex bradycardic response. Overall, these findings provide evidence that hippocampal nitrergic mechanisms acting in a NOS isoform-specific manner plays a prominent role in control of baroreflex function. Indeed, the results indicate that nNOS and iNOS exerts an inhibitory influence on reflex bradycardia, whereas iNOS mediates the reflex tachycardia.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual PaulistaLaboratory of Pharmacology São Paulo State University (UNESP) School of Pharmaceutical SciencesJoint UFSCar-UNESP Graduate Program in Physiological SciencesLaboratory of Pharmacology São Paulo State University (UNESP) School of Pharmaceutical SciencesJoint UFSCar-UNESP Graduate Program in Physiological SciencesCNPq: 141992/2016-6FAPESP: 2015/05922-9FAPESP: 2017/19249-0CNPq: 456405/2014-3Universidade Estadual Paulista (Unesp)Almeida, Jeferson [UNESP]Oliveira, Leandro A. [UNESP]Benini, Ricardo [UNESP]Crestani, Carlos C. [UNESP]2019-10-06T15:29:26Z2019-10-06T15:29:26Z2019-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article109-116http://dx.doi.org/10.1016/j.brainres.2018.12.044Brain Research, v. 1710, p. 109-116.1872-62400006-8993http://hdl.handle.net/11449/18722710.1016/j.brainres.2018.12.0442-s2.0-85059431029Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrain Researchinfo:eu-repo/semantics/openAccess2021-10-22T23:59:51Zoai:repositorio.unesp.br:11449/187227Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:09:39.209014Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Differential roles of hippocampal nNOS and iNOS in the control of baroreflex function in conscious rats |
title |
Differential roles of hippocampal nNOS and iNOS in the control of baroreflex function in conscious rats |
spellingShingle |
Differential roles of hippocampal nNOS and iNOS in the control of baroreflex function in conscious rats Almeida, Jeferson [UNESP] 1400 W Arterial pressure Cardiovascular Heart rate N-Propyl-L-arginine NO |
title_short |
Differential roles of hippocampal nNOS and iNOS in the control of baroreflex function in conscious rats |
title_full |
Differential roles of hippocampal nNOS and iNOS in the control of baroreflex function in conscious rats |
title_fullStr |
Differential roles of hippocampal nNOS and iNOS in the control of baroreflex function in conscious rats |
title_full_unstemmed |
Differential roles of hippocampal nNOS and iNOS in the control of baroreflex function in conscious rats |
title_sort |
Differential roles of hippocampal nNOS and iNOS in the control of baroreflex function in conscious rats |
author |
Almeida, Jeferson [UNESP] |
author_facet |
Almeida, Jeferson [UNESP] Oliveira, Leandro A. [UNESP] Benini, Ricardo [UNESP] Crestani, Carlos C. [UNESP] |
author_role |
author |
author2 |
Oliveira, Leandro A. [UNESP] Benini, Ricardo [UNESP] Crestani, Carlos C. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Almeida, Jeferson [UNESP] Oliveira, Leandro A. [UNESP] Benini, Ricardo [UNESP] Crestani, Carlos C. [UNESP] |
dc.subject.por.fl_str_mv |
1400 W Arterial pressure Cardiovascular Heart rate N-Propyl-L-arginine NO |
topic |
1400 W Arterial pressure Cardiovascular Heart rate N-Propyl-L-arginine NO |
description |
The baroreflex is a prominent moment-to-moment mechanism regulating the blood pressure. The hippocampus is a limbic structure in which has been pointed out as part of central network regulating baroreflex. However, the local neurochemical mechanisms involved in control of baroreflex function are not completely understood. Thus, this study aimed to investigate the involvement of nitrergic neurotransmission present in the dorsal hippocampus in baroreflex control of heart rate in conscious rats. For this, we evaluated the effect of bilateral microinjection into the dorsal hippocampus of either the nitric oxide (NO) scavenger carboxy-PTIO, the selective neuronal nitric oxide synthase (nNOS) inhibitor Nω-Propyl-L-arginine (NPLA) or the selective inducible nitric oxide synthase (iNOS) inhibitor 1400 W in bradycardia evoked by blood pressure increases in response to intravenous infusion of phenylephrine, and tachycardia caused by blood pressure decreases evoked by intravenous infusion of sodium nitroprusside. Bilateral microinjection of carboxy-PTIO into the dorsal hippocampus decreased the baroreflex tachycardic response without affecting the reflex bradycardia. Hippocampus treatment with NPLA increased the baroreflex bradycardia gain without affecting the reflex tachycardia. Bilateral hippocampal treatment with 1400 W decreased the reflex tachycardia and increased the baroreflex bradycardic response. Overall, these findings provide evidence that hippocampal nitrergic mechanisms acting in a NOS isoform-specific manner plays a prominent role in control of baroreflex function. Indeed, the results indicate that nNOS and iNOS exerts an inhibitory influence on reflex bradycardia, whereas iNOS mediates the reflex tachycardia. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T15:29:26Z 2019-10-06T15:29:26Z 2019-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.brainres.2018.12.044 Brain Research, v. 1710, p. 109-116. 1872-6240 0006-8993 http://hdl.handle.net/11449/187227 10.1016/j.brainres.2018.12.044 2-s2.0-85059431029 |
url |
http://dx.doi.org/10.1016/j.brainres.2018.12.044 http://hdl.handle.net/11449/187227 |
identifier_str_mv |
Brain Research, v. 1710, p. 109-116. 1872-6240 0006-8993 10.1016/j.brainres.2018.12.044 2-s2.0-85059431029 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brain Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
109-116 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128763797438464 |