Micropropagación de Pothomorphe umbellata (L.) Miq. vía organogénesis directa
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | spa |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://scielo.sld.cu/scielo.php?script=sci_abstract&pid=S1028-47962007000400006&lng=es&nrm=iso&tlng=en http://hdl.handle.net/11449/69922 |
Resumo: | The present research was aimed at standardizing the protocol of seed disinfection, seed germination and organogenesis via callus of Pothomorphe umbellata (L.) Miq.The germinated seeds were inoculated in different concentrations of BAP (benzylamine purine) and NAA (naphthalene acetic acid) in order to stimulate the callus induction. After 60 days of culture, the calluses with some shoots were taken to an organogenesis medium (GA3 0.1 mg.L -1, BAP 0.5 mg.L-1) during 40 days, to be transferred later to a development medium. Finally, the plantules were acclimatized, presenting a good index of survival. |
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Micropropagación de Pothomorphe umbellata (L.) Miq. vía organogénesis directaMicropropagation of Pothomorphe umbellata (L.) Miq. via direct organogenesisGrowth regulatorsMedicinal plantsPothomorphe umbellataLepianthes peltataThe present research was aimed at standardizing the protocol of seed disinfection, seed germination and organogenesis via callus of Pothomorphe umbellata (L.) Miq.The germinated seeds were inoculated in different concentrations of BAP (benzylamine purine) and NAA (naphthalene acetic acid) in order to stimulate the callus induction. After 60 days of culture, the calluses with some shoots were taken to an organogenesis medium (GA3 0.1 mg.L -1, BAP 0.5 mg.L-1) during 40 days, to be transferred later to a development medium. Finally, the plantules were acclimatized, presenting a good index of survival.Laboratório de Micropropagação e Análises Bioquímicas Instituto de Biociências Universidade Estadual Paulista (UNESP), Botucatu, São PauloLaboratório de Micropropagação e Análises Químicas Instituto de Biociências Universidade Estadual Paulista, UNESP, Botucatu, São PauloUniversidade Estadual Paulista (UNESP), Botucatu, São PauloLaboratório de Micropropagação e Análises Bioquímicas Instituto de Biociências Universidade Estadual Paulista (UNESP), Botucatu, São PauloLaboratório de Micropropagação e Análises Químicas Instituto de Biociências Universidade Estadual Paulista, UNESP, Botucatu, São PauloUniversidade Estadual Paulista (UNESP), Botucatu, São PauloUniversidade Estadual Paulista (Unesp)Borda-Yepez, Charlotte Cesty [UNESP]Tejada, Erik C. Saenz [UNESP]Lima, Giuseppina Pace Pereira [UNESP]2014-05-27T11:22:36Z2014-05-27T11:22:36Z2007-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://scielo.sld.cu/scielo.php?script=sci_abstract&pid=S1028-47962007000400006&lng=es&nrm=iso&tlng=enRevista Cubana de Plantas Medicinales, v. 12, n. 4, 2007.1028-4796http://hdl.handle.net/11449/69922S1028-479620070004000062-s2.0-397491220772-s2.0-39749122077.pdf8104143593771412Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPspaRevista Cubana de Plantas Medicinales0,127info:eu-repo/semantics/openAccess2023-10-15T06:03:38Zoai:repositorio.unesp.br:11449/69922Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:56:47.273324Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Micropropagación de Pothomorphe umbellata (L.) Miq. vía organogénesis directa Micropropagation of Pothomorphe umbellata (L.) Miq. via direct organogenesis |
title |
Micropropagación de Pothomorphe umbellata (L.) Miq. vía organogénesis directa |
spellingShingle |
Micropropagación de Pothomorphe umbellata (L.) Miq. vía organogénesis directa Borda-Yepez, Charlotte Cesty [UNESP] Growth regulators Medicinal plants Pothomorphe umbellata Lepianthes peltata |
title_short |
Micropropagación de Pothomorphe umbellata (L.) Miq. vía organogénesis directa |
title_full |
Micropropagación de Pothomorphe umbellata (L.) Miq. vía organogénesis directa |
title_fullStr |
Micropropagación de Pothomorphe umbellata (L.) Miq. vía organogénesis directa |
title_full_unstemmed |
Micropropagación de Pothomorphe umbellata (L.) Miq. vía organogénesis directa |
title_sort |
Micropropagación de Pothomorphe umbellata (L.) Miq. vía organogénesis directa |
author |
Borda-Yepez, Charlotte Cesty [UNESP] |
author_facet |
Borda-Yepez, Charlotte Cesty [UNESP] Tejada, Erik C. Saenz [UNESP] Lima, Giuseppina Pace Pereira [UNESP] |
author_role |
author |
author2 |
Tejada, Erik C. Saenz [UNESP] Lima, Giuseppina Pace Pereira [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Borda-Yepez, Charlotte Cesty [UNESP] Tejada, Erik C. Saenz [UNESP] Lima, Giuseppina Pace Pereira [UNESP] |
dc.subject.por.fl_str_mv |
Growth regulators Medicinal plants Pothomorphe umbellata Lepianthes peltata |
topic |
Growth regulators Medicinal plants Pothomorphe umbellata Lepianthes peltata |
description |
The present research was aimed at standardizing the protocol of seed disinfection, seed germination and organogenesis via callus of Pothomorphe umbellata (L.) Miq.The germinated seeds were inoculated in different concentrations of BAP (benzylamine purine) and NAA (naphthalene acetic acid) in order to stimulate the callus induction. After 60 days of culture, the calluses with some shoots were taken to an organogenesis medium (GA3 0.1 mg.L -1, BAP 0.5 mg.L-1) during 40 days, to be transferred later to a development medium. Finally, the plantules were acclimatized, presenting a good index of survival. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-10-01 2014-05-27T11:22:36Z 2014-05-27T11:22:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://scielo.sld.cu/scielo.php?script=sci_abstract&pid=S1028-47962007000400006&lng=es&nrm=iso&tlng=en Revista Cubana de Plantas Medicinales, v. 12, n. 4, 2007. 1028-4796 http://hdl.handle.net/11449/69922 S1028-47962007000400006 2-s2.0-39749122077 2-s2.0-39749122077.pdf 8104143593771412 |
url |
http://scielo.sld.cu/scielo.php?script=sci_abstract&pid=S1028-47962007000400006&lng=es&nrm=iso&tlng=en http://hdl.handle.net/11449/69922 |
identifier_str_mv |
Revista Cubana de Plantas Medicinales, v. 12, n. 4, 2007. 1028-4796 S1028-47962007000400006 2-s2.0-39749122077 2-s2.0-39749122077.pdf 8104143593771412 |
dc.language.iso.fl_str_mv |
spa |
language |
spa |
dc.relation.none.fl_str_mv |
Revista Cubana de Plantas Medicinales 0,127 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128439456104448 |