Eucalyptus ESTs corresponding to the enzyme glutamine synthetase and the protein D1, sites of action of herbicides that cause oxidative stress
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1415-47572005000400010 http://hdl.handle.net/11449/68573 |
Resumo: | This work was aimed at locating Eucalyptus ESTs corresponding to the GS enzyme (Glutamine Synthetase, EC = 6.3.1.2) and to the D1 protein, which are directly related to resistance to herbicides that promote oxidative stress. Glutamine Synthetase corresponds to the site of action of the herbicide glufosinate. Herbicides that belong to groups such as ureas, uracils, triazines and triazinones act on the D1-Qb complex (receptor of electrons from the Photosystem II) by inactivating it. The clusters EGEQRT3302E01.g, EGEQRT3001F12.b; EGEZLV1203B04.g; EGBGFB1211H06.g and EGEZLV1205F09.g enclosed complete sequences (with 356 amino acids) of the Glutamine Synthetase enzyme. The cluster EGEQSL1054G06.g is a consensus of four reads and enclosed a complete sequence of D1 Protein (with 353 amino acids). The comparison of the sequences of Protein D1 from different species showed that the substitutions of serine (S) by glycine (G) or serine (S) by threonine (T) at the position 264 could produce plants resistant to herbicides that act on electron flow on Photosystem II. The sequence of amino acids corresponding to the cluster EGEQSL1054G06.g had a serine in position 264 indicating sensitivity of the Eucalyptus plants to herbicides that act on this site. Copyright by the Brazilian Society of Genetics. |
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Eucalyptus ESTs corresponding to the enzyme glutamine synthetase and the protein D1, sites of action of herbicides that cause oxidative stressD1 proteinEucalyptusGlutamine synthetaseHerbicideamino acidglutamate ammonia ligaseglycineherbicideserinethreoninetriazine derivativeuracil derivativeureaamino acid sequenceamino acid substitutionconsensus sequencecontrolled studyelectron transportenzyme active siteenzyme localizationexpressed sequence taggene clusternonhumannucleotide sequenceoxidative stressphotosystem IIplant geneticssensitivity analysisspecies differenceThis work was aimed at locating Eucalyptus ESTs corresponding to the GS enzyme (Glutamine Synthetase, EC = 6.3.1.2) and to the D1 protein, which are directly related to resistance to herbicides that promote oxidative stress. Glutamine Synthetase corresponds to the site of action of the herbicide glufosinate. Herbicides that belong to groups such as ureas, uracils, triazines and triazinones act on the D1-Qb complex (receptor of electrons from the Photosystem II) by inactivating it. The clusters EGEQRT3302E01.g, EGEQRT3001F12.b; EGEZLV1203B04.g; EGBGFB1211H06.g and EGEZLV1205F09.g enclosed complete sequences (with 356 amino acids) of the Glutamine Synthetase enzyme. The cluster EGEQSL1054G06.g is a consensus of four reads and enclosed a complete sequence of D1 Protein (with 353 amino acids). The comparison of the sequences of Protein D1 from different species showed that the substitutions of serine (S) by glycine (G) or serine (S) by threonine (T) at the position 264 could produce plants resistant to herbicides that act on electron flow on Photosystem II. The sequence of amino acids corresponding to the cluster EGEQSL1054G06.g had a serine in position 264 indicating sensitivity of the Eucalyptus plants to herbicides that act on this site. Copyright by the Brazilian Society of Genetics.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Agrárias Laboratório de Motología, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Laboratório de Xenobióticos, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Laboratôrio de Genética, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade Ciências Agrárias Laboratório de Melhoramento Vegetal, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade Ciências Agrárias Laboratório de Fitopatologia, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade Ciências Agrárias Laboratório de Solos, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade Ciências Agrárias Laboratório de Entomologia, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Agrárias Laboratório de Motología, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Laboratório de Xenobióticos, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Laboratôrio de Genética, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade Ciências Agrárias Laboratório de Melhoramento Vegetal, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade Ciências Agrárias Laboratório de Fitopatologia, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade Ciências Agrárias Laboratório de Solos, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade Ciências Agrárias Laboratório de Entomologia, 18603-970 Botucatu, SPUniversidade Estadual Paulista (Unesp)Velini, Edivaldo Domingues [UNESP]Trindade, Maria Lúcia Bueno [UNESP]Alves, Elza [UNESP]Catâneo, Ana Catarina [UNESP]Marino, Celso Luis [UNESP]de Godoy Maia, Ivan [UNESP]Mori, Edson Seizo [UNESP]Furtado, Edson Luiz [UNESP]Guerrini, Iraê Amaral [UNESP]Wilcken, Carlos Frederico [UNESP]2014-05-27T11:21:43Z2014-05-27T11:21:43Z2005-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article555-561application/pdfhttp://dx.doi.org/10.1590/S1415-47572005000400010Genetics and Molecular Biology, v. 28, n. 3 SUPPL., p. 555-561, 2005.1415-47571678-4685http://hdl.handle.net/11449/6857310.1590/S1415-47572005000400010S1415-47572005000400010WOS:0002043898000102-s2.0-308444715442-s2.0-30844471544.pdf864922209917616284590425674861090165348738208319985549344816170238459894858333950000-0003-0431-59420000-0002-6924-835X0000-0003-4524-954XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2024-06-07T13:57:54Zoai:repositorio.unesp.br:11449/68573Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:29:59.875405Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Eucalyptus ESTs corresponding to the enzyme glutamine synthetase and the protein D1, sites of action of herbicides that cause oxidative stress |
title |
Eucalyptus ESTs corresponding to the enzyme glutamine synthetase and the protein D1, sites of action of herbicides that cause oxidative stress |
spellingShingle |
Eucalyptus ESTs corresponding to the enzyme glutamine synthetase and the protein D1, sites of action of herbicides that cause oxidative stress Velini, Edivaldo Domingues [UNESP] D1 protein Eucalyptus Glutamine synthetase Herbicide amino acid glutamate ammonia ligase glycine herbicide serine threonine triazine derivative uracil derivative urea amino acid sequence amino acid substitution consensus sequence controlled study electron transport enzyme active site enzyme localization expressed sequence tag gene cluster nonhuman nucleotide sequence oxidative stress photosystem II plant genetics sensitivity analysis species difference |
title_short |
Eucalyptus ESTs corresponding to the enzyme glutamine synthetase and the protein D1, sites of action of herbicides that cause oxidative stress |
title_full |
Eucalyptus ESTs corresponding to the enzyme glutamine synthetase and the protein D1, sites of action of herbicides that cause oxidative stress |
title_fullStr |
Eucalyptus ESTs corresponding to the enzyme glutamine synthetase and the protein D1, sites of action of herbicides that cause oxidative stress |
title_full_unstemmed |
Eucalyptus ESTs corresponding to the enzyme glutamine synthetase and the protein D1, sites of action of herbicides that cause oxidative stress |
title_sort |
Eucalyptus ESTs corresponding to the enzyme glutamine synthetase and the protein D1, sites of action of herbicides that cause oxidative stress |
author |
Velini, Edivaldo Domingues [UNESP] |
author_facet |
Velini, Edivaldo Domingues [UNESP] Trindade, Maria Lúcia Bueno [UNESP] Alves, Elza [UNESP] Catâneo, Ana Catarina [UNESP] Marino, Celso Luis [UNESP] de Godoy Maia, Ivan [UNESP] Mori, Edson Seizo [UNESP] Furtado, Edson Luiz [UNESP] Guerrini, Iraê Amaral [UNESP] Wilcken, Carlos Frederico [UNESP] |
author_role |
author |
author2 |
Trindade, Maria Lúcia Bueno [UNESP] Alves, Elza [UNESP] Catâneo, Ana Catarina [UNESP] Marino, Celso Luis [UNESP] de Godoy Maia, Ivan [UNESP] Mori, Edson Seizo [UNESP] Furtado, Edson Luiz [UNESP] Guerrini, Iraê Amaral [UNESP] Wilcken, Carlos Frederico [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Velini, Edivaldo Domingues [UNESP] Trindade, Maria Lúcia Bueno [UNESP] Alves, Elza [UNESP] Catâneo, Ana Catarina [UNESP] Marino, Celso Luis [UNESP] de Godoy Maia, Ivan [UNESP] Mori, Edson Seizo [UNESP] Furtado, Edson Luiz [UNESP] Guerrini, Iraê Amaral [UNESP] Wilcken, Carlos Frederico [UNESP] |
dc.subject.por.fl_str_mv |
D1 protein Eucalyptus Glutamine synthetase Herbicide amino acid glutamate ammonia ligase glycine herbicide serine threonine triazine derivative uracil derivative urea amino acid sequence amino acid substitution consensus sequence controlled study electron transport enzyme active site enzyme localization expressed sequence tag gene cluster nonhuman nucleotide sequence oxidative stress photosystem II plant genetics sensitivity analysis species difference |
topic |
D1 protein Eucalyptus Glutamine synthetase Herbicide amino acid glutamate ammonia ligase glycine herbicide serine threonine triazine derivative uracil derivative urea amino acid sequence amino acid substitution consensus sequence controlled study electron transport enzyme active site enzyme localization expressed sequence tag gene cluster nonhuman nucleotide sequence oxidative stress photosystem II plant genetics sensitivity analysis species difference |
description |
This work was aimed at locating Eucalyptus ESTs corresponding to the GS enzyme (Glutamine Synthetase, EC = 6.3.1.2) and to the D1 protein, which are directly related to resistance to herbicides that promote oxidative stress. Glutamine Synthetase corresponds to the site of action of the herbicide glufosinate. Herbicides that belong to groups such as ureas, uracils, triazines and triazinones act on the D1-Qb complex (receptor of electrons from the Photosystem II) by inactivating it. The clusters EGEQRT3302E01.g, EGEQRT3001F12.b; EGEZLV1203B04.g; EGBGFB1211H06.g and EGEZLV1205F09.g enclosed complete sequences (with 356 amino acids) of the Glutamine Synthetase enzyme. The cluster EGEQSL1054G06.g is a consensus of four reads and enclosed a complete sequence of D1 Protein (with 353 amino acids). The comparison of the sequences of Protein D1 from different species showed that the substitutions of serine (S) by glycine (G) or serine (S) by threonine (T) at the position 264 could produce plants resistant to herbicides that act on electron flow on Photosystem II. The sequence of amino acids corresponding to the cluster EGEQSL1054G06.g had a serine in position 264 indicating sensitivity of the Eucalyptus plants to herbicides that act on this site. Copyright by the Brazilian Society of Genetics. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-12-01 2014-05-27T11:21:43Z 2014-05-27T11:21:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1415-47572005000400010 Genetics and Molecular Biology, v. 28, n. 3 SUPPL., p. 555-561, 2005. 1415-4757 1678-4685 http://hdl.handle.net/11449/68573 10.1590/S1415-47572005000400010 S1415-47572005000400010 WOS:000204389800010 2-s2.0-30844471544 2-s2.0-30844471544.pdf 8649222099176162 8459042567486109 0165348738208319 9855493448161702 3845989485833395 0000-0003-0431-5942 0000-0002-6924-835X 0000-0003-4524-954X |
url |
http://dx.doi.org/10.1590/S1415-47572005000400010 http://hdl.handle.net/11449/68573 |
identifier_str_mv |
Genetics and Molecular Biology, v. 28, n. 3 SUPPL., p. 555-561, 2005. 1415-4757 1678-4685 10.1590/S1415-47572005000400010 S1415-47572005000400010 WOS:000204389800010 2-s2.0-30844471544 2-s2.0-30844471544.pdf 8649222099176162 8459042567486109 0165348738208319 9855493448161702 3845989485833395 0000-0003-0431-5942 0000-0002-6924-835X 0000-0003-4524-954X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Biology 1.493 0,638 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
555-561 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129526356508672 |