Evaluation of Two Configurations of Hydroxyapatite and Beta-Tricalcium Phosphate in Sinus Grafts with Simultaneous Implant Installation: An Experimental Study in Rabbits

Detalhes bibliográficos
Autor(a) principal: Jacob, Ricardo Garcia Mureb [UNESP]
Data de Publicação: 2023
Outros Autores: Ervolino da Silva, Ana Cláudia [UNESP], Chaushu, Liat, Lang, Niklaus Peter, Borges Duailibe de Deus, Ciro [UNESP], Botticelli, Daniele, Rangel Garcia Júnior, Idelmo [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/dj11050121
http://hdl.handle.net/11449/247448
Resumo: Background: This study aimed to evaluate peri-implant bone formation in rabbits after sinus grafting mediated by hydroxyapatite and beta-tricalcium phosphate (HA + β-TCP) in granule or paste configurations, concomitant with immediate implant installation. Material & methods: Thirty-four rabbit maxillary sinuses were grafted with HA + β-TCP, half of which were applied in a granule and half in a paste composition. Implant placement was performed simultaneously. At 7 and 40 days postoperatively, the animals were euthanized, and samples were prepared for tomographic, microtomographic, histological, histometric (hematoxylin and eosin staining, HE), and immunohistochemical (labeling of transcription factor Runx-2 [RUNX2], vascular endothelial growth factor [VEGF], osteocalcin [OCN], and tartrate-resistant acid phosphatase [TRAP]) analysis. Implant removal torque was also measured. Results: On tomography, maintenance of sinus membrane integrity was observed in both the groups. Higher values of morphometric parameters evaluated by micro-CT were found in the “paste group” after seven days. At 40 days, there were no significant differences between the groups in most of the microtomographic parameters evaluated. In histological sections stained with HE, a higher percentage of newly formed bone was observed in the “granule group” after 40 days. Similar positive immunolabeling was observed for both RUNX2 and OCN in both the experimental groups. TRAP immunolabeling was similar in both groups as well. VEGF labeling increased in the “granule group”, indicating a higher osteoconductive potential in this biomaterial. Similar removal torque values were observed in both groups. Thus, the two HA + β-TCP configurations showed similar healing patterns of simultaneously installed implants adjacent to sinus floor elevation. However, significantly higher bone values were observed for the “granule configuration”. Conclusions: The HA + β-TCP granules and paste presentations showed favorable long-term healing results, with bone formation in similar quantities and quality adjacent to the implants.
id UNSP_b8a90280940da2d75bf1e0a5e182b8aa
oai_identifier_str oai:repositorio.unesp.br:11449/247448
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Evaluation of Two Configurations of Hydroxyapatite and Beta-Tricalcium Phosphate in Sinus Grafts with Simultaneous Implant Installation: An Experimental Study in Rabbitsbone regenerationdental implantrabbitsinus floor augmentationBackground: This study aimed to evaluate peri-implant bone formation in rabbits after sinus grafting mediated by hydroxyapatite and beta-tricalcium phosphate (HA + β-TCP) in granule or paste configurations, concomitant with immediate implant installation. Material & methods: Thirty-four rabbit maxillary sinuses were grafted with HA + β-TCP, half of which were applied in a granule and half in a paste composition. Implant placement was performed simultaneously. At 7 and 40 days postoperatively, the animals were euthanized, and samples were prepared for tomographic, microtomographic, histological, histometric (hematoxylin and eosin staining, HE), and immunohistochemical (labeling of transcription factor Runx-2 [RUNX2], vascular endothelial growth factor [VEGF], osteocalcin [OCN], and tartrate-resistant acid phosphatase [TRAP]) analysis. Implant removal torque was also measured. Results: On tomography, maintenance of sinus membrane integrity was observed in both the groups. Higher values of morphometric parameters evaluated by micro-CT were found in the “paste group” after seven days. At 40 days, there were no significant differences between the groups in most of the microtomographic parameters evaluated. In histological sections stained with HE, a higher percentage of newly formed bone was observed in the “granule group” after 40 days. Similar positive immunolabeling was observed for both RUNX2 and OCN in both the experimental groups. TRAP immunolabeling was similar in both groups as well. VEGF labeling increased in the “granule group”, indicating a higher osteoconductive potential in this biomaterial. Similar removal torque values were observed in both groups. Thus, the two HA + β-TCP configurations showed similar healing patterns of simultaneously installed implants adjacent to sinus floor elevation. However, significantly higher bone values were observed for the “granule configuration”. Conclusions: The HA + β-TCP granules and paste presentations showed favorable long-term healing results, with bone formation in similar quantities and quality adjacent to the implants.Department of Diagnosis and Surgery School of Dentistry São Paulo State University (UNESP), Rua José Bonifácio 1193, SPDepartment of Basic Sciences School of Dentistry São Paulo State University (UNESP), Rua José Bonifácio 1193, SPDepartment of Periodontology and Implant Dentistry The Maurice and Gabriela Goldschleger School of Dentistry Tel Aviv UniversitySchool of Dental Medicine University of BerneARDEC AcademyDepartment of Diagnosis and Surgery School of Dentistry São Paulo State University (UNESP), Rua José Bonifácio 1193, SPDepartment of Basic Sciences School of Dentistry São Paulo State University (UNESP), Rua José Bonifácio 1193, SPUniversidade Estadual Paulista (UNESP)Tel Aviv UniversityUniversity of BerneARDEC AcademyJacob, Ricardo Garcia Mureb [UNESP]Ervolino da Silva, Ana Cláudia [UNESP]Chaushu, LiatLang, Niklaus PeterBorges Duailibe de Deus, Ciro [UNESP]Botticelli, DanieleRangel Garcia Júnior, Idelmo [UNESP]2023-07-29T13:16:18Z2023-07-29T13:16:18Z2023-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/dj11050121Dentistry Journal, v. 11, n. 5, 2023.2304-6767http://hdl.handle.net/11449/24744810.3390/dj110501212-s2.0-85160249149Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDentistry Journalinfo:eu-repo/semantics/openAccess2023-07-29T13:16:18Zoai:repositorio.unesp.br:11449/247448Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:26:16.504778Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Evaluation of Two Configurations of Hydroxyapatite and Beta-Tricalcium Phosphate in Sinus Grafts with Simultaneous Implant Installation: An Experimental Study in Rabbits
title Evaluation of Two Configurations of Hydroxyapatite and Beta-Tricalcium Phosphate in Sinus Grafts with Simultaneous Implant Installation: An Experimental Study in Rabbits
spellingShingle Evaluation of Two Configurations of Hydroxyapatite and Beta-Tricalcium Phosphate in Sinus Grafts with Simultaneous Implant Installation: An Experimental Study in Rabbits
Jacob, Ricardo Garcia Mureb [UNESP]
bone regeneration
dental implant
rabbit
sinus floor augmentation
title_short Evaluation of Two Configurations of Hydroxyapatite and Beta-Tricalcium Phosphate in Sinus Grafts with Simultaneous Implant Installation: An Experimental Study in Rabbits
title_full Evaluation of Two Configurations of Hydroxyapatite and Beta-Tricalcium Phosphate in Sinus Grafts with Simultaneous Implant Installation: An Experimental Study in Rabbits
title_fullStr Evaluation of Two Configurations of Hydroxyapatite and Beta-Tricalcium Phosphate in Sinus Grafts with Simultaneous Implant Installation: An Experimental Study in Rabbits
title_full_unstemmed Evaluation of Two Configurations of Hydroxyapatite and Beta-Tricalcium Phosphate in Sinus Grafts with Simultaneous Implant Installation: An Experimental Study in Rabbits
title_sort Evaluation of Two Configurations of Hydroxyapatite and Beta-Tricalcium Phosphate in Sinus Grafts with Simultaneous Implant Installation: An Experimental Study in Rabbits
author Jacob, Ricardo Garcia Mureb [UNESP]
author_facet Jacob, Ricardo Garcia Mureb [UNESP]
Ervolino da Silva, Ana Cláudia [UNESP]
Chaushu, Liat
Lang, Niklaus Peter
Borges Duailibe de Deus, Ciro [UNESP]
Botticelli, Daniele
Rangel Garcia Júnior, Idelmo [UNESP]
author_role author
author2 Ervolino da Silva, Ana Cláudia [UNESP]
Chaushu, Liat
Lang, Niklaus Peter
Borges Duailibe de Deus, Ciro [UNESP]
Botticelli, Daniele
Rangel Garcia Júnior, Idelmo [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Tel Aviv University
University of Berne
ARDEC Academy
dc.contributor.author.fl_str_mv Jacob, Ricardo Garcia Mureb [UNESP]
Ervolino da Silva, Ana Cláudia [UNESP]
Chaushu, Liat
Lang, Niklaus Peter
Borges Duailibe de Deus, Ciro [UNESP]
Botticelli, Daniele
Rangel Garcia Júnior, Idelmo [UNESP]
dc.subject.por.fl_str_mv bone regeneration
dental implant
rabbit
sinus floor augmentation
topic bone regeneration
dental implant
rabbit
sinus floor augmentation
description Background: This study aimed to evaluate peri-implant bone formation in rabbits after sinus grafting mediated by hydroxyapatite and beta-tricalcium phosphate (HA + β-TCP) in granule or paste configurations, concomitant with immediate implant installation. Material & methods: Thirty-four rabbit maxillary sinuses were grafted with HA + β-TCP, half of which were applied in a granule and half in a paste composition. Implant placement was performed simultaneously. At 7 and 40 days postoperatively, the animals were euthanized, and samples were prepared for tomographic, microtomographic, histological, histometric (hematoxylin and eosin staining, HE), and immunohistochemical (labeling of transcription factor Runx-2 [RUNX2], vascular endothelial growth factor [VEGF], osteocalcin [OCN], and tartrate-resistant acid phosphatase [TRAP]) analysis. Implant removal torque was also measured. Results: On tomography, maintenance of sinus membrane integrity was observed in both the groups. Higher values of morphometric parameters evaluated by micro-CT were found in the “paste group” after seven days. At 40 days, there were no significant differences between the groups in most of the microtomographic parameters evaluated. In histological sections stained with HE, a higher percentage of newly formed bone was observed in the “granule group” after 40 days. Similar positive immunolabeling was observed for both RUNX2 and OCN in both the experimental groups. TRAP immunolabeling was similar in both groups as well. VEGF labeling increased in the “granule group”, indicating a higher osteoconductive potential in this biomaterial. Similar removal torque values were observed in both groups. Thus, the two HA + β-TCP configurations showed similar healing patterns of simultaneously installed implants adjacent to sinus floor elevation. However, significantly higher bone values were observed for the “granule configuration”. Conclusions: The HA + β-TCP granules and paste presentations showed favorable long-term healing results, with bone formation in similar quantities and quality adjacent to the implants.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T13:16:18Z
2023-07-29T13:16:18Z
2023-05-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/dj11050121
Dentistry Journal, v. 11, n. 5, 2023.
2304-6767
http://hdl.handle.net/11449/247448
10.3390/dj11050121
2-s2.0-85160249149
url http://dx.doi.org/10.3390/dj11050121
http://hdl.handle.net/11449/247448
identifier_str_mv Dentistry Journal, v. 11, n. 5, 2023.
2304-6767
10.3390/dj11050121
2-s2.0-85160249149
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Dentistry Journal
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129202112692224