Damage-regulated autophagy modulator 1 in oral inflammation and infection
Main Author: | |
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Publication Date: | 2018 |
Other Authors: | , , , , , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1007/s00784-018-2381-6 http://hdl.handle.net/11449/175875 |
Summary: | Objectives: Damage-regulated autophagy modulator (DRAM) 1 is a p53 target gene with possible involvement in oral inflammation and infection. This study sought to examine the presence and regulation of DRAM1 in periodontal diseases. Material and methods: In vitro, human periodontal ligament fibroblasts were exposed to interleukin (IL)-1β and Fusobacterium nucleatum for up to 2 days. The DRAM1 synthesis and its regulation were analyzed by real-time PCR, immunocytochemistry, and ELISA. Expressions of other autophagy-associated genes were also studied by real-time PCR. In vivo, synthesis of DRAM1 in gingival biopsies from rats and patients with and without periodontal disease was examined by real-time PCR and immunohistochemistry. For statistics, ANOVA and post-hoc tests were applied (p < 0.05). Results: In vitro, DRAM1 was significantly upregulated by IL-1β and F. nucleatum over 2 days and a wide range of concentrations. Additionally, increased DRAM1 protein levels in response to both stimulants were observed. Autophagy-associated genes ATG3, BAK1, HDAC6, and IRGM were also upregulated under inflammatory or infectious conditions. In vivo, the DRAM1 gene expression was significantly enhanced in rat gingival biopsies with induced periodontitis as compared to control. Significantly increased DRAM1 levels were also detected in human gingival biopsies from sites of periodontitis as compared to healthy sites. Conclusion: Our data provide novel evidence that DRAM1 is increased under inflammatory and infectious conditions in periodontal cells and tissues, suggesting a pivotal role of DRAM1 in oral inflammation and infection. Clinical relevance: DRAM1 might be a promising target in future diagnostic and treatment strategies for periodontitis. |
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Damage-regulated autophagy modulator 1 in oral inflammation and infectionAutophagyDamage-regulated autophagy modulatorFusobacterium nucleatumInterleukin-1βPeriodontal ligamentPeriodontitisObjectives: Damage-regulated autophagy modulator (DRAM) 1 is a p53 target gene with possible involvement in oral inflammation and infection. This study sought to examine the presence and regulation of DRAM1 in periodontal diseases. Material and methods: In vitro, human periodontal ligament fibroblasts were exposed to interleukin (IL)-1β and Fusobacterium nucleatum for up to 2 days. The DRAM1 synthesis and its regulation were analyzed by real-time PCR, immunocytochemistry, and ELISA. Expressions of other autophagy-associated genes were also studied by real-time PCR. In vivo, synthesis of DRAM1 in gingival biopsies from rats and patients with and without periodontal disease was examined by real-time PCR and immunohistochemistry. For statistics, ANOVA and post-hoc tests were applied (p < 0.05). Results: In vitro, DRAM1 was significantly upregulated by IL-1β and F. nucleatum over 2 days and a wide range of concentrations. Additionally, increased DRAM1 protein levels in response to both stimulants were observed. Autophagy-associated genes ATG3, BAK1, HDAC6, and IRGM were also upregulated under inflammatory or infectious conditions. In vivo, the DRAM1 gene expression was significantly enhanced in rat gingival biopsies with induced periodontitis as compared to control. Significantly increased DRAM1 levels were also detected in human gingival biopsies from sites of periodontitis as compared to healthy sites. Conclusion: Our data provide novel evidence that DRAM1 is increased under inflammatory and infectious conditions in periodontal cells and tissues, suggesting a pivotal role of DRAM1 in oral inflammation and infection. Clinical relevance: DRAM1 might be a promising target in future diagnostic and treatment strategies for periodontitis.Section of Experimental Dento-Maxillo-Facial Medicine Center of Dento-Maxillo-Facial Medicine University of Bonn, Welschnonnenstr. 17Department of Orthodontics Center of Dento-Maxillo-Facial Medicine University of BonnDepartment of Diagnosis and Surgery School of Dentistry at Araraquara Sao Paulo State University UNESPDepartment of Periodontology Laboratory for Oral Microbiology School of Dental Medicine University of BernDepartment/Discipline of Periodontics Faculty of Dentistry The University of SydneyInstitute of Reconstructive Neurobiology Life & Brain Center University of BonnNoel Martin Visiting Chair Faculty of Dentistry University of SydneyDepartment of Diagnosis and Surgery School of Dentistry at Araraquara Sao Paulo State University UNESPUniversity of BonnUniversidade Estadual Paulista (Unesp)University of BernThe University of SydneyUniversity of SydneyMemmert, SvenjaNogueira, A. V.B. [UNESP]Damanaki, A.Nokhbehsaim, M.Eick, S.Divnic-Resnik, T.Spahr, A.Rath-Deschner, B.Till, A.Götz, W.Cirelli, J. A. [UNESP]Jäger, A.Deschner, J.2018-12-11T17:17:58Z2018-12-11T17:17:58Z2018-02-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-9application/pdfhttp://dx.doi.org/10.1007/s00784-018-2381-6Clinical Oral Investigations, p. 1-9.1436-37711432-6981http://hdl.handle.net/11449/17587510.1007/s00784-018-2381-62-s2.0-850419115182-s2.0-85041911518.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigations0,9860,986info:eu-repo/semantics/openAccess2024-01-21T06:24:51Zoai:repositorio.unesp.br:11449/175875Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-21T06:24:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Damage-regulated autophagy modulator 1 in oral inflammation and infection |
title |
Damage-regulated autophagy modulator 1 in oral inflammation and infection |
spellingShingle |
Damage-regulated autophagy modulator 1 in oral inflammation and infection Memmert, Svenja Autophagy Damage-regulated autophagy modulator Fusobacterium nucleatum Interleukin-1β Periodontal ligament Periodontitis |
title_short |
Damage-regulated autophagy modulator 1 in oral inflammation and infection |
title_full |
Damage-regulated autophagy modulator 1 in oral inflammation and infection |
title_fullStr |
Damage-regulated autophagy modulator 1 in oral inflammation and infection |
title_full_unstemmed |
Damage-regulated autophagy modulator 1 in oral inflammation and infection |
title_sort |
Damage-regulated autophagy modulator 1 in oral inflammation and infection |
author |
Memmert, Svenja |
author_facet |
Memmert, Svenja Nogueira, A. V.B. [UNESP] Damanaki, A. Nokhbehsaim, M. Eick, S. Divnic-Resnik, T. Spahr, A. Rath-Deschner, B. Till, A. Götz, W. Cirelli, J. A. [UNESP] Jäger, A. Deschner, J. |
author_role |
author |
author2 |
Nogueira, A. V.B. [UNESP] Damanaki, A. Nokhbehsaim, M. Eick, S. Divnic-Resnik, T. Spahr, A. Rath-Deschner, B. Till, A. Götz, W. Cirelli, J. A. [UNESP] Jäger, A. Deschner, J. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Bonn Universidade Estadual Paulista (Unesp) University of Bern The University of Sydney University of Sydney |
dc.contributor.author.fl_str_mv |
Memmert, Svenja Nogueira, A. V.B. [UNESP] Damanaki, A. Nokhbehsaim, M. Eick, S. Divnic-Resnik, T. Spahr, A. Rath-Deschner, B. Till, A. Götz, W. Cirelli, J. A. [UNESP] Jäger, A. Deschner, J. |
dc.subject.por.fl_str_mv |
Autophagy Damage-regulated autophagy modulator Fusobacterium nucleatum Interleukin-1β Periodontal ligament Periodontitis |
topic |
Autophagy Damage-regulated autophagy modulator Fusobacterium nucleatum Interleukin-1β Periodontal ligament Periodontitis |
description |
Objectives: Damage-regulated autophagy modulator (DRAM) 1 is a p53 target gene with possible involvement in oral inflammation and infection. This study sought to examine the presence and regulation of DRAM1 in periodontal diseases. Material and methods: In vitro, human periodontal ligament fibroblasts were exposed to interleukin (IL)-1β and Fusobacterium nucleatum for up to 2 days. The DRAM1 synthesis and its regulation were analyzed by real-time PCR, immunocytochemistry, and ELISA. Expressions of other autophagy-associated genes were also studied by real-time PCR. In vivo, synthesis of DRAM1 in gingival biopsies from rats and patients with and without periodontal disease was examined by real-time PCR and immunohistochemistry. For statistics, ANOVA and post-hoc tests were applied (p < 0.05). Results: In vitro, DRAM1 was significantly upregulated by IL-1β and F. nucleatum over 2 days and a wide range of concentrations. Additionally, increased DRAM1 protein levels in response to both stimulants were observed. Autophagy-associated genes ATG3, BAK1, HDAC6, and IRGM were also upregulated under inflammatory or infectious conditions. In vivo, the DRAM1 gene expression was significantly enhanced in rat gingival biopsies with induced periodontitis as compared to control. Significantly increased DRAM1 levels were also detected in human gingival biopsies from sites of periodontitis as compared to healthy sites. Conclusion: Our data provide novel evidence that DRAM1 is increased under inflammatory and infectious conditions in periodontal cells and tissues, suggesting a pivotal role of DRAM1 in oral inflammation and infection. Clinical relevance: DRAM1 might be a promising target in future diagnostic and treatment strategies for periodontitis. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:17:58Z 2018-12-11T17:17:58Z 2018-02-13 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-018-2381-6 Clinical Oral Investigations, p. 1-9. 1436-3771 1432-6981 http://hdl.handle.net/11449/175875 10.1007/s00784-018-2381-6 2-s2.0-85041911518 2-s2.0-85041911518.pdf |
url |
http://dx.doi.org/10.1007/s00784-018-2381-6 http://hdl.handle.net/11449/175875 |
identifier_str_mv |
Clinical Oral Investigations, p. 1-9. 1436-3771 1432-6981 10.1007/s00784-018-2381-6 2-s2.0-85041911518 2-s2.0-85041911518.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations 0,986 0,986 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-9 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965686182707200 |