Diferenciação osteogênica de células mesenquimais do cordão umbilical canino

Detalhes bibliográficos
Autor(a) principal: Souza, Talita Floering Brêda [UNESP]
Data de Publicação: 2013
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/121939
Resumo: This study aimed to isolate MSCs from canine Wharton's jelly umbilical cord and evaluate in vitro the effect of PRP and the MOD in osteogenic differentiation of these cells in the absence of osteogenic medium. Cells were isolated and characterized as undifferentiated mesenchymal cells with a phenotype fibroblasts-like and characteristic phenotype of confirmed by flow cytometry with CD44 +, CD105 +, CD271 +, CD34-and CD45-, plus the ability to differentiate into adipocytes, chondrocytes and osteocytes. Except for the control group (MSCs), in all groups, G-PRP (PRP and MSCs), G-DBM (DBM and MSCs) and G-PRP + DBM (MSCs and PRP + DBM) was differentiation osteoblastic with production of mineralized bone matrix confirmed by Alizarin red staining. The measurement of alkaline phosphatase (AP) increased up to 14 days in all groups. Bone proteins were expressed in the polymerase chain reaction in real time and were able to confirm the differentiation bone in all groups. Osteopontin had its greatest expression in G- PRP to seven days followed by G-PRP + DBM, at other times the G-PRP + DBM had higher expression than the other groups. The osteocalcin and Runx2 were expressed more intensely in the G-PRP + DBM at the end of the experiment. It can be concluded that the PRP and DBM are capable of promoting osteogenesis mesenchymal cells in Wharton's jelly of the umbilical cord with canine, with advantage in the presence of DBM
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spelling Diferenciação osteogênica de células mesenquimais do cordão umbilical caninoCãoFator de crescimento transformador betaOssos - CrescimentoPlasma Rico em PlaquetasCélulas mesenquimais estromaisGeleia de WhartonCordão umbilicalCanine umbilical cordThis study aimed to isolate MSCs from canine Wharton's jelly umbilical cord and evaluate in vitro the effect of PRP and the MOD in osteogenic differentiation of these cells in the absence of osteogenic medium. Cells were isolated and characterized as undifferentiated mesenchymal cells with a phenotype fibroblasts-like and characteristic phenotype of confirmed by flow cytometry with CD44 +, CD105 +, CD271 +, CD34-and CD45-, plus the ability to differentiate into adipocytes, chondrocytes and osteocytes. Except for the control group (MSCs), in all groups, G-PRP (PRP and MSCs), G-DBM (DBM and MSCs) and G-PRP + DBM (MSCs and PRP + DBM) was differentiation osteoblastic with production of mineralized bone matrix confirmed by Alizarin red staining. The measurement of alkaline phosphatase (AP) increased up to 14 days in all groups. Bone proteins were expressed in the polymerase chain reaction in real time and were able to confirm the differentiation bone in all groups. Osteopontin had its greatest expression in G- PRP to seven days followed by G-PRP + DBM, at other times the G-PRP + DBM had higher expression than the other groups. The osteocalcin and Runx2 were expressed more intensely in the G-PRP + DBM at the end of the experiment. It can be concluded that the PRP and DBM are capable of promoting osteogenesis mesenchymal cells in Wharton's jelly of the umbilical cord with canine, with advantage in the presence of DBMUniversidade Estadual Paulista (Unesp)Andrade, Alexandre Lima de [UNESP]Universidade Estadual Paulista (Unesp)Souza, Talita Floering Brêda [UNESP]2015-04-09T12:28:12Z2015-04-09T12:28:12Z2013-08-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis72 f.application/pdfSOUZA, Talita Floering Brêda. Diferenciação osteogênica de células mesenquimais do cordão umbilical canino. 2013. 72 f. , 2013.http://hdl.handle.net/11449/121939000814146000814146.pdf33004021075P86404176495306171Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2023-12-09T06:20:53Zoai:repositorio.unesp.br:11449/121939Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:51:13.537048Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Diferenciação osteogênica de células mesenquimais do cordão umbilical canino
title Diferenciação osteogênica de células mesenquimais do cordão umbilical canino
spellingShingle Diferenciação osteogênica de células mesenquimais do cordão umbilical canino
Souza, Talita Floering Brêda [UNESP]
Cão
Fator de crescimento transformador beta
Ossos - Crescimento
Plasma Rico em Plaquetas
Células mesenquimais estromais
Geleia de Wharton
Cordão umbilical
Canine umbilical cord
title_short Diferenciação osteogênica de células mesenquimais do cordão umbilical canino
title_full Diferenciação osteogênica de células mesenquimais do cordão umbilical canino
title_fullStr Diferenciação osteogênica de células mesenquimais do cordão umbilical canino
title_full_unstemmed Diferenciação osteogênica de células mesenquimais do cordão umbilical canino
title_sort Diferenciação osteogênica de células mesenquimais do cordão umbilical canino
author Souza, Talita Floering Brêda [UNESP]
author_facet Souza, Talita Floering Brêda [UNESP]
author_role author
dc.contributor.none.fl_str_mv Andrade, Alexandre Lima de [UNESP]
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Souza, Talita Floering Brêda [UNESP]
dc.subject.por.fl_str_mv Cão
Fator de crescimento transformador beta
Ossos - Crescimento
Plasma Rico em Plaquetas
Células mesenquimais estromais
Geleia de Wharton
Cordão umbilical
Canine umbilical cord
topic Cão
Fator de crescimento transformador beta
Ossos - Crescimento
Plasma Rico em Plaquetas
Células mesenquimais estromais
Geleia de Wharton
Cordão umbilical
Canine umbilical cord
description This study aimed to isolate MSCs from canine Wharton's jelly umbilical cord and evaluate in vitro the effect of PRP and the MOD in osteogenic differentiation of these cells in the absence of osteogenic medium. Cells were isolated and characterized as undifferentiated mesenchymal cells with a phenotype fibroblasts-like and characteristic phenotype of confirmed by flow cytometry with CD44 +, CD105 +, CD271 +, CD34-and CD45-, plus the ability to differentiate into adipocytes, chondrocytes and osteocytes. Except for the control group (MSCs), in all groups, G-PRP (PRP and MSCs), G-DBM (DBM and MSCs) and G-PRP + DBM (MSCs and PRP + DBM) was differentiation osteoblastic with production of mineralized bone matrix confirmed by Alizarin red staining. The measurement of alkaline phosphatase (AP) increased up to 14 days in all groups. Bone proteins were expressed in the polymerase chain reaction in real time and were able to confirm the differentiation bone in all groups. Osteopontin had its greatest expression in G- PRP to seven days followed by G-PRP + DBM, at other times the G-PRP + DBM had higher expression than the other groups. The osteocalcin and Runx2 were expressed more intensely in the G-PRP + DBM at the end of the experiment. It can be concluded that the PRP and DBM are capable of promoting osteogenesis mesenchymal cells in Wharton's jelly of the umbilical cord with canine, with advantage in the presence of DBM
publishDate 2013
dc.date.none.fl_str_mv 2013-08-30
2015-04-09T12:28:12Z
2015-04-09T12:28:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv SOUZA, Talita Floering Brêda. Diferenciação osteogênica de células mesenquimais do cordão umbilical canino. 2013. 72 f. , 2013.
http://hdl.handle.net/11449/121939
000814146
000814146.pdf
33004021075P8
6404176495306171
identifier_str_mv SOUZA, Talita Floering Brêda. Diferenciação osteogênica de células mesenquimais do cordão umbilical canino. 2013. 72 f. , 2013.
000814146
000814146.pdf
33004021075P8
6404176495306171
url http://hdl.handle.net/11449/121939
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 72 f.
application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.source.none.fl_str_mv Aleph
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129129339420672