Diferenciação osteogênica de células mesenquimais do cordão umbilical canino
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/121939 |
Resumo: | This study aimed to isolate MSCs from canine Wharton's jelly umbilical cord and evaluate in vitro the effect of PRP and the MOD in osteogenic differentiation of these cells in the absence of osteogenic medium. Cells were isolated and characterized as undifferentiated mesenchymal cells with a phenotype fibroblasts-like and characteristic phenotype of confirmed by flow cytometry with CD44 +, CD105 +, CD271 +, CD34-and CD45-, plus the ability to differentiate into adipocytes, chondrocytes and osteocytes. Except for the control group (MSCs), in all groups, G-PRP (PRP and MSCs), G-DBM (DBM and MSCs) and G-PRP + DBM (MSCs and PRP + DBM) was differentiation osteoblastic with production of mineralized bone matrix confirmed by Alizarin red staining. The measurement of alkaline phosphatase (AP) increased up to 14 days in all groups. Bone proteins were expressed in the polymerase chain reaction in real time and were able to confirm the differentiation bone in all groups. Osteopontin had its greatest expression in G- PRP to seven days followed by G-PRP + DBM, at other times the G-PRP + DBM had higher expression than the other groups. The osteocalcin and Runx2 were expressed more intensely in the G-PRP + DBM at the end of the experiment. It can be concluded that the PRP and DBM are capable of promoting osteogenesis mesenchymal cells in Wharton's jelly of the umbilical cord with canine, with advantage in the presence of DBM |
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Repositório Institucional da UNESP |
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Diferenciação osteogênica de células mesenquimais do cordão umbilical caninoCãoFator de crescimento transformador betaOssos - CrescimentoPlasma Rico em PlaquetasCélulas mesenquimais estromaisGeleia de WhartonCordão umbilicalCanine umbilical cordThis study aimed to isolate MSCs from canine Wharton's jelly umbilical cord and evaluate in vitro the effect of PRP and the MOD in osteogenic differentiation of these cells in the absence of osteogenic medium. Cells were isolated and characterized as undifferentiated mesenchymal cells with a phenotype fibroblasts-like and characteristic phenotype of confirmed by flow cytometry with CD44 +, CD105 +, CD271 +, CD34-and CD45-, plus the ability to differentiate into adipocytes, chondrocytes and osteocytes. Except for the control group (MSCs), in all groups, G-PRP (PRP and MSCs), G-DBM (DBM and MSCs) and G-PRP + DBM (MSCs and PRP + DBM) was differentiation osteoblastic with production of mineralized bone matrix confirmed by Alizarin red staining. The measurement of alkaline phosphatase (AP) increased up to 14 days in all groups. Bone proteins were expressed in the polymerase chain reaction in real time and were able to confirm the differentiation bone in all groups. Osteopontin had its greatest expression in G- PRP to seven days followed by G-PRP + DBM, at other times the G-PRP + DBM had higher expression than the other groups. The osteocalcin and Runx2 were expressed more intensely in the G-PRP + DBM at the end of the experiment. It can be concluded that the PRP and DBM are capable of promoting osteogenesis mesenchymal cells in Wharton's jelly of the umbilical cord with canine, with advantage in the presence of DBMUniversidade Estadual Paulista (Unesp)Andrade, Alexandre Lima de [UNESP]Universidade Estadual Paulista (Unesp)Souza, Talita Floering Brêda [UNESP]2015-04-09T12:28:12Z2015-04-09T12:28:12Z2013-08-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis72 f.application/pdfSOUZA, Talita Floering Brêda. Diferenciação osteogênica de células mesenquimais do cordão umbilical canino. 2013. 72 f. , 2013.http://hdl.handle.net/11449/121939000814146000814146.pdf33004021075P86404176495306171Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2023-12-09T06:20:53Zoai:repositorio.unesp.br:11449/121939Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:51:13.537048Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Diferenciação osteogênica de células mesenquimais do cordão umbilical canino |
title |
Diferenciação osteogênica de células mesenquimais do cordão umbilical canino |
spellingShingle |
Diferenciação osteogênica de células mesenquimais do cordão umbilical canino Souza, Talita Floering Brêda [UNESP] Cão Fator de crescimento transformador beta Ossos - Crescimento Plasma Rico em Plaquetas Células mesenquimais estromais Geleia de Wharton Cordão umbilical Canine umbilical cord |
title_short |
Diferenciação osteogênica de células mesenquimais do cordão umbilical canino |
title_full |
Diferenciação osteogênica de células mesenquimais do cordão umbilical canino |
title_fullStr |
Diferenciação osteogênica de células mesenquimais do cordão umbilical canino |
title_full_unstemmed |
Diferenciação osteogênica de células mesenquimais do cordão umbilical canino |
title_sort |
Diferenciação osteogênica de células mesenquimais do cordão umbilical canino |
author |
Souza, Talita Floering Brêda [UNESP] |
author_facet |
Souza, Talita Floering Brêda [UNESP] |
author_role |
author |
dc.contributor.none.fl_str_mv |
Andrade, Alexandre Lima de [UNESP] Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Souza, Talita Floering Brêda [UNESP] |
dc.subject.por.fl_str_mv |
Cão Fator de crescimento transformador beta Ossos - Crescimento Plasma Rico em Plaquetas Células mesenquimais estromais Geleia de Wharton Cordão umbilical Canine umbilical cord |
topic |
Cão Fator de crescimento transformador beta Ossos - Crescimento Plasma Rico em Plaquetas Células mesenquimais estromais Geleia de Wharton Cordão umbilical Canine umbilical cord |
description |
This study aimed to isolate MSCs from canine Wharton's jelly umbilical cord and evaluate in vitro the effect of PRP and the MOD in osteogenic differentiation of these cells in the absence of osteogenic medium. Cells were isolated and characterized as undifferentiated mesenchymal cells with a phenotype fibroblasts-like and characteristic phenotype of confirmed by flow cytometry with CD44 +, CD105 +, CD271 +, CD34-and CD45-, plus the ability to differentiate into adipocytes, chondrocytes and osteocytes. Except for the control group (MSCs), in all groups, G-PRP (PRP and MSCs), G-DBM (DBM and MSCs) and G-PRP + DBM (MSCs and PRP + DBM) was differentiation osteoblastic with production of mineralized bone matrix confirmed by Alizarin red staining. The measurement of alkaline phosphatase (AP) increased up to 14 days in all groups. Bone proteins were expressed in the polymerase chain reaction in real time and were able to confirm the differentiation bone in all groups. Osteopontin had its greatest expression in G- PRP to seven days followed by G-PRP + DBM, at other times the G-PRP + DBM had higher expression than the other groups. The osteocalcin and Runx2 were expressed more intensely in the G-PRP + DBM at the end of the experiment. It can be concluded that the PRP and DBM are capable of promoting osteogenesis mesenchymal cells in Wharton's jelly of the umbilical cord with canine, with advantage in the presence of DBM |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-08-30 2015-04-09T12:28:12Z 2015-04-09T12:28:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SOUZA, Talita Floering Brêda. Diferenciação osteogênica de células mesenquimais do cordão umbilical canino. 2013. 72 f. , 2013. http://hdl.handle.net/11449/121939 000814146 000814146.pdf 33004021075P8 6404176495306171 |
identifier_str_mv |
SOUZA, Talita Floering Brêda. Diferenciação osteogênica de células mesenquimais do cordão umbilical canino. 2013. 72 f. , 2013. 000814146 000814146.pdf 33004021075P8 6404176495306171 |
url |
http://hdl.handle.net/11449/121939 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
72 f. application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.source.none.fl_str_mv |
Aleph reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129129339420672 |