Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1415-47572003000200003 http://hdl.handle.net/11449/17935 |
Resumo: | Head and neck carcinomas represent the sixth most frequent type of cancer in the world, and 90% are derived from squamous cells (HNSCC). In this study of 15 HNSCC cases, extensive aneuploidy was detected by G banding in most tumors. The most frequently observed numerical changes involved gain of a chromosome 22, and loss of chromosomes Y, 10, 17, and 19. The most frequent structural alteration was del(22)(q13.1). As compared to G-banding, fluorescence in situ hybridization (FISH) proved to be an effective technique for detecting aneuploidy. Interphase FISH with a chromosome 17 centromere probe disclosed a high frequency of monosomy for chromosome 17, in contrast with G-banding, by which clonal monosomy 17 was detected in only three of the tumors. Painting probes for chromosomes 5 and 16 were used to evaluate a selected series of HNSCC in which G-banding analysis had shown marker chromosomes. FISH analysis failed to confirm the origin of the marker chromosomes, but four out of five cases showed a significant loss of chromosomes 5. This difference between FISH and G-banding results may reflect the smaller number of metaphase analyzed as well as the criteria adopted for sorting these metaphases. Therefore results obtained solely by G-banding analysis should be considered with caution. Our data confirmed the involvement of chromosome 17 in head and neck squamous cell carcinomas. |
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Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomasFISHchromosomal aberrationshead and neck cancerchromosome 17Head and neck carcinomas represent the sixth most frequent type of cancer in the world, and 90% are derived from squamous cells (HNSCC). In this study of 15 HNSCC cases, extensive aneuploidy was detected by G banding in most tumors. The most frequently observed numerical changes involved gain of a chromosome 22, and loss of chromosomes Y, 10, 17, and 19. The most frequent structural alteration was del(22)(q13.1). As compared to G-banding, fluorescence in situ hybridization (FISH) proved to be an effective technique for detecting aneuploidy. Interphase FISH with a chromosome 17 centromere probe disclosed a high frequency of monosomy for chromosome 17, in contrast with G-banding, by which clonal monosomy 17 was detected in only three of the tumors. Painting probes for chromosomes 5 and 16 were used to evaluate a selected series of HNSCC in which G-banding analysis had shown marker chromosomes. FISH analysis failed to confirm the origin of the marker chromosomes, but four out of five cases showed a significant loss of chromosomes 5. This difference between FISH and G-banding results may reflect the smaller number of metaphase analyzed as well as the criteria adopted for sorting these metaphases. Therefore results obtained solely by G-banding analysis should be considered with caution. Our data confirmed the involvement of chromosome 17 in head and neck squamous cell carcinomas.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)UNESP Instituto de Biociências Departamento de GenéticaHospital A.C. Camargo Departamento de Cirurgia de Cabeça e PescoçoUNESP Instituto de Biociências Departamento de GenéticaSociedade Brasileira de GenéticaUniversidade Estadual Paulista (Unesp)Hospital A.C. Camargo Departamento de Cirurgia de Cabeça e PescoçoVeiga, Luciana CS [UNESP]Bérgamo, Nádia A [UNESP]Kowalski, Luiz PauloRogatto, Silvia Regina [UNESP]2014-05-20T13:50:13Z2014-05-20T13:50:13Z2003-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article121-128application/pdfhttp://dx.doi.org/10.1590/S1415-47572003000200003Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 26, n. 2, p. 121-128, 2003.1415-4757http://hdl.handle.net/11449/1793510.1590/S1415-47572003000200003S1415-47572003000200003S1415-47572003000200003.pdf2259986546265579SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2024-09-03T14:29:56Zoai:repositorio.unesp.br:11449/17935Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T14:29:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas |
title |
Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas |
spellingShingle |
Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas Veiga, Luciana CS [UNESP] FISH chromosomal aberrations head and neck cancer chromosome 17 |
title_short |
Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas |
title_full |
Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas |
title_fullStr |
Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas |
title_full_unstemmed |
Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas |
title_sort |
Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas |
author |
Veiga, Luciana CS [UNESP] |
author_facet |
Veiga, Luciana CS [UNESP] Bérgamo, Nádia A [UNESP] Kowalski, Luiz Paulo Rogatto, Silvia Regina [UNESP] |
author_role |
author |
author2 |
Bérgamo, Nádia A [UNESP] Kowalski, Luiz Paulo Rogatto, Silvia Regina [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Hospital A.C. Camargo Departamento de Cirurgia de Cabeça e Pescoço |
dc.contributor.author.fl_str_mv |
Veiga, Luciana CS [UNESP] Bérgamo, Nádia A [UNESP] Kowalski, Luiz Paulo Rogatto, Silvia Regina [UNESP] |
dc.subject.por.fl_str_mv |
FISH chromosomal aberrations head and neck cancer chromosome 17 |
topic |
FISH chromosomal aberrations head and neck cancer chromosome 17 |
description |
Head and neck carcinomas represent the sixth most frequent type of cancer in the world, and 90% are derived from squamous cells (HNSCC). In this study of 15 HNSCC cases, extensive aneuploidy was detected by G banding in most tumors. The most frequently observed numerical changes involved gain of a chromosome 22, and loss of chromosomes Y, 10, 17, and 19. The most frequent structural alteration was del(22)(q13.1). As compared to G-banding, fluorescence in situ hybridization (FISH) proved to be an effective technique for detecting aneuploidy. Interphase FISH with a chromosome 17 centromere probe disclosed a high frequency of monosomy for chromosome 17, in contrast with G-banding, by which clonal monosomy 17 was detected in only three of the tumors. Painting probes for chromosomes 5 and 16 were used to evaluate a selected series of HNSCC in which G-banding analysis had shown marker chromosomes. FISH analysis failed to confirm the origin of the marker chromosomes, but four out of five cases showed a significant loss of chromosomes 5. This difference between FISH and G-banding results may reflect the smaller number of metaphase analyzed as well as the criteria adopted for sorting these metaphases. Therefore results obtained solely by G-banding analysis should be considered with caution. Our data confirmed the involvement of chromosome 17 in head and neck squamous cell carcinomas. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-01-01 2014-05-20T13:50:13Z 2014-05-20T13:50:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1415-47572003000200003 Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 26, n. 2, p. 121-128, 2003. 1415-4757 http://hdl.handle.net/11449/17935 10.1590/S1415-47572003000200003 S1415-47572003000200003 S1415-47572003000200003.pdf 2259986546265579 |
url |
http://dx.doi.org/10.1590/S1415-47572003000200003 http://hdl.handle.net/11449/17935 |
identifier_str_mv |
Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 26, n. 2, p. 121-128, 2003. 1415-4757 10.1590/S1415-47572003000200003 S1415-47572003000200003 S1415-47572003000200003.pdf 2259986546265579 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Biology 1.493 0,638 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
121-128 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021361096065024 |