Genotoxicity assessment of fipronil (frontline plus®) in Canis familiaris
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-736X2017000300009 http://hdl.handle.net/11449/174778 |
Resumo: | Fipronil is a pesticide widely used for controlling fleas and ticks in domestic animals, and its short-term exposure can lead to serious effects on animals. However, the possible genotoxic effect of this compound has not been investigated in target animals. Based on the hypothesis that fipronil can induce genotoxicity, this study evaluated the effect of fipronil on DNA damage in peripheral blood cells. For that purpose, ten dogs of both sexes were used in the study. The product (6.7mg/kg) was applied on the dorsal neck region of each animal. Peripheral blood samples were collected immediately prior to application of the product, and at 3, 8 and 24 hours after the application. Samples were processed for comet assay. No statistically significant differences were found among the four time points. The current study suggests for the first time that a single exposure to this pesticide does not induce systemic genotoxic effect in dogs. |
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Genotoxicity assessment of fipronil (frontline plus®) in Canis familiarisComet assayDNA damageDogsFipronilFrontline plus®PesticideFipronil is a pesticide widely used for controlling fleas and ticks in domestic animals, and its short-term exposure can lead to serious effects on animals. However, the possible genotoxic effect of this compound has not been investigated in target animals. Based on the hypothesis that fipronil can induce genotoxicity, this study evaluated the effect of fipronil on DNA damage in peripheral blood cells. For that purpose, ten dogs of both sexes were used in the study. The product (6.7mg/kg) was applied on the dorsal neck region of each animal. Peripheral blood samples were collected immediately prior to application of the product, and at 3, 8 and 24 hours after the application. Samples were processed for comet assay. No statistically significant differences were found among the four time points. The current study suggests for the first time that a single exposure to this pesticide does not induce systemic genotoxic effect in dogs.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Clínica Cirúrgica de Pequenos Animais Departamento de Medicina Veterinária Universidade Estadual do Centro-Oeste (Unicentro), Rua Simeão Camargo Varela de Sá 3Departamento de Cirurgia e Anestesiologia Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (Unesp), Distrito de Rubião Júnior s/nDepartamento de Anestesiologia Faculdade de Medicina de Botucatu Unesp, Av. Prof. Mario Rubens Guimaraes Montenegro s/nDepartamento de Cirurgia e Anestesiologia Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (Unesp), Distrito de Rubião Júnior s/nDepartamento de Anestesiologia Faculdade de Medicina de Botucatu Unesp, Av. Prof. Mario Rubens Guimaraes Montenegro s/nUniversidade Estadual do Centro-Oeste (Unicentro)Universidade Estadual Paulista (Unesp)Ziliotto, LianeLuna, Stelio P.L. [UNESP]Filho, Daniel A.A. [UNESP]Resende, Ludimila O. [UNESP]Aun, Aline G. [UNESP]Braz, Mariana G. [UNESP]2018-12-11T17:12:49Z2018-12-11T17:12:49Z2017-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article257-260application/pdfhttp://dx.doi.org/10.1590/S0100-736X2017000300009Pesquisa Veterinaria Brasileira, v. 37, n. 3, p. 257-260, 2017.1678-51500100-736Xhttp://hdl.handle.net/11449/17477810.1590/S0100-736X2017000300009S0100-736X20170003002572-s2.0-85020987737S0100-736X2017000300257.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPesquisa Veterinaria Brasileirainfo:eu-repo/semantics/openAccess2024-08-14T13:20:38Zoai:repositorio.unesp.br:11449/174778Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T13:20:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genotoxicity assessment of fipronil (frontline plus®) in Canis familiaris |
title |
Genotoxicity assessment of fipronil (frontline plus®) in Canis familiaris |
spellingShingle |
Genotoxicity assessment of fipronil (frontline plus®) in Canis familiaris Ziliotto, Liane Comet assay DNA damage Dogs Fipronil Frontline plus® Pesticide |
title_short |
Genotoxicity assessment of fipronil (frontline plus®) in Canis familiaris |
title_full |
Genotoxicity assessment of fipronil (frontline plus®) in Canis familiaris |
title_fullStr |
Genotoxicity assessment of fipronil (frontline plus®) in Canis familiaris |
title_full_unstemmed |
Genotoxicity assessment of fipronil (frontline plus®) in Canis familiaris |
title_sort |
Genotoxicity assessment of fipronil (frontline plus®) in Canis familiaris |
author |
Ziliotto, Liane |
author_facet |
Ziliotto, Liane Luna, Stelio P.L. [UNESP] Filho, Daniel A.A. [UNESP] Resende, Ludimila O. [UNESP] Aun, Aline G. [UNESP] Braz, Mariana G. [UNESP] |
author_role |
author |
author2 |
Luna, Stelio P.L. [UNESP] Filho, Daniel A.A. [UNESP] Resende, Ludimila O. [UNESP] Aun, Aline G. [UNESP] Braz, Mariana G. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual do Centro-Oeste (Unicentro) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ziliotto, Liane Luna, Stelio P.L. [UNESP] Filho, Daniel A.A. [UNESP] Resende, Ludimila O. [UNESP] Aun, Aline G. [UNESP] Braz, Mariana G. [UNESP] |
dc.subject.por.fl_str_mv |
Comet assay DNA damage Dogs Fipronil Frontline plus® Pesticide |
topic |
Comet assay DNA damage Dogs Fipronil Frontline plus® Pesticide |
description |
Fipronil is a pesticide widely used for controlling fleas and ticks in domestic animals, and its short-term exposure can lead to serious effects on animals. However, the possible genotoxic effect of this compound has not been investigated in target animals. Based on the hypothesis that fipronil can induce genotoxicity, this study evaluated the effect of fipronil on DNA damage in peripheral blood cells. For that purpose, ten dogs of both sexes were used in the study. The product (6.7mg/kg) was applied on the dorsal neck region of each animal. Peripheral blood samples were collected immediately prior to application of the product, and at 3, 8 and 24 hours after the application. Samples were processed for comet assay. No statistically significant differences were found among the four time points. The current study suggests for the first time that a single exposure to this pesticide does not induce systemic genotoxic effect in dogs. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03-01 2018-12-11T17:12:49Z 2018-12-11T17:12:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-736X2017000300009 Pesquisa Veterinaria Brasileira, v. 37, n. 3, p. 257-260, 2017. 1678-5150 0100-736X http://hdl.handle.net/11449/174778 10.1590/S0100-736X2017000300009 S0100-736X2017000300257 2-s2.0-85020987737 S0100-736X2017000300257.pdf |
url |
http://dx.doi.org/10.1590/S0100-736X2017000300009 http://hdl.handle.net/11449/174778 |
identifier_str_mv |
Pesquisa Veterinaria Brasileira, v. 37, n. 3, p. 257-260, 2017. 1678-5150 0100-736X 10.1590/S0100-736X2017000300009 S0100-736X2017000300257 2-s2.0-85020987737 S0100-736X2017000300257.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pesquisa Veterinaria Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
257-260 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128176131407872 |