Genotoxicity assessment of fipronil (frontline plus®) in Canis familiaris

Detalhes bibliográficos
Autor(a) principal: Ziliotto, Liane
Data de Publicação: 2017
Outros Autores: Luna, Stelio P.L. [UNESP], Filho, Daniel A.A. [UNESP], Resende, Ludimila O. [UNESP], Aun, Aline G. [UNESP], Braz, Mariana G. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0100-736X2017000300009
http://hdl.handle.net/11449/174778
Resumo: Fipronil is a pesticide widely used for controlling fleas and ticks in domestic animals, and its short-term exposure can lead to serious effects on animals. However, the possible genotoxic effect of this compound has not been investigated in target animals. Based on the hypothesis that fipronil can induce genotoxicity, this study evaluated the effect of fipronil on DNA damage in peripheral blood cells. For that purpose, ten dogs of both sexes were used in the study. The product (6.7mg/kg) was applied on the dorsal neck region of each animal. Peripheral blood samples were collected immediately prior to application of the product, and at 3, 8 and 24 hours after the application. Samples were processed for comet assay. No statistically significant differences were found among the four time points. The current study suggests for the first time that a single exposure to this pesticide does not induce systemic genotoxic effect in dogs.
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spelling Genotoxicity assessment of fipronil (frontline plus®) in Canis familiarisComet assayDNA damageDogsFipronilFrontline plus®PesticideFipronil is a pesticide widely used for controlling fleas and ticks in domestic animals, and its short-term exposure can lead to serious effects on animals. However, the possible genotoxic effect of this compound has not been investigated in target animals. Based on the hypothesis that fipronil can induce genotoxicity, this study evaluated the effect of fipronil on DNA damage in peripheral blood cells. For that purpose, ten dogs of both sexes were used in the study. The product (6.7mg/kg) was applied on the dorsal neck region of each animal. Peripheral blood samples were collected immediately prior to application of the product, and at 3, 8 and 24 hours after the application. Samples were processed for comet assay. No statistically significant differences were found among the four time points. The current study suggests for the first time that a single exposure to this pesticide does not induce systemic genotoxic effect in dogs.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Clínica Cirúrgica de Pequenos Animais Departamento de Medicina Veterinária Universidade Estadual do Centro-Oeste (Unicentro), Rua Simeão Camargo Varela de Sá 3Departamento de Cirurgia e Anestesiologia Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (Unesp), Distrito de Rubião Júnior s/nDepartamento de Anestesiologia Faculdade de Medicina de Botucatu Unesp, Av. Prof. Mario Rubens Guimaraes Montenegro s/nDepartamento de Cirurgia e Anestesiologia Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (Unesp), Distrito de Rubião Júnior s/nDepartamento de Anestesiologia Faculdade de Medicina de Botucatu Unesp, Av. Prof. Mario Rubens Guimaraes Montenegro s/nUniversidade Estadual do Centro-Oeste (Unicentro)Universidade Estadual Paulista (Unesp)Ziliotto, LianeLuna, Stelio P.L. [UNESP]Filho, Daniel A.A. [UNESP]Resende, Ludimila O. [UNESP]Aun, Aline G. [UNESP]Braz, Mariana G. [UNESP]2018-12-11T17:12:49Z2018-12-11T17:12:49Z2017-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article257-260application/pdfhttp://dx.doi.org/10.1590/S0100-736X2017000300009Pesquisa Veterinaria Brasileira, v. 37, n. 3, p. 257-260, 2017.1678-51500100-736Xhttp://hdl.handle.net/11449/17477810.1590/S0100-736X2017000300009S0100-736X20170003002572-s2.0-85020987737S0100-736X2017000300257.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPesquisa Veterinaria Brasileirainfo:eu-repo/semantics/openAccess2024-08-14T13:20:38Zoai:repositorio.unesp.br:11449/174778Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T13:20:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Genotoxicity assessment of fipronil (frontline plus®) in Canis familiaris
title Genotoxicity assessment of fipronil (frontline plus®) in Canis familiaris
spellingShingle Genotoxicity assessment of fipronil (frontline plus®) in Canis familiaris
Ziliotto, Liane
Comet assay
DNA damage
Dogs
Fipronil
Frontline plus®
Pesticide
title_short Genotoxicity assessment of fipronil (frontline plus®) in Canis familiaris
title_full Genotoxicity assessment of fipronil (frontline plus®) in Canis familiaris
title_fullStr Genotoxicity assessment of fipronil (frontline plus®) in Canis familiaris
title_full_unstemmed Genotoxicity assessment of fipronil (frontline plus®) in Canis familiaris
title_sort Genotoxicity assessment of fipronil (frontline plus®) in Canis familiaris
author Ziliotto, Liane
author_facet Ziliotto, Liane
Luna, Stelio P.L. [UNESP]
Filho, Daniel A.A. [UNESP]
Resende, Ludimila O. [UNESP]
Aun, Aline G. [UNESP]
Braz, Mariana G. [UNESP]
author_role author
author2 Luna, Stelio P.L. [UNESP]
Filho, Daniel A.A. [UNESP]
Resende, Ludimila O. [UNESP]
Aun, Aline G. [UNESP]
Braz, Mariana G. [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual do Centro-Oeste (Unicentro)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Ziliotto, Liane
Luna, Stelio P.L. [UNESP]
Filho, Daniel A.A. [UNESP]
Resende, Ludimila O. [UNESP]
Aun, Aline G. [UNESP]
Braz, Mariana G. [UNESP]
dc.subject.por.fl_str_mv Comet assay
DNA damage
Dogs
Fipronil
Frontline plus®
Pesticide
topic Comet assay
DNA damage
Dogs
Fipronil
Frontline plus®
Pesticide
description Fipronil is a pesticide widely used for controlling fleas and ticks in domestic animals, and its short-term exposure can lead to serious effects on animals. However, the possible genotoxic effect of this compound has not been investigated in target animals. Based on the hypothesis that fipronil can induce genotoxicity, this study evaluated the effect of fipronil on DNA damage in peripheral blood cells. For that purpose, ten dogs of both sexes were used in the study. The product (6.7mg/kg) was applied on the dorsal neck region of each animal. Peripheral blood samples were collected immediately prior to application of the product, and at 3, 8 and 24 hours after the application. Samples were processed for comet assay. No statistically significant differences were found among the four time points. The current study suggests for the first time that a single exposure to this pesticide does not induce systemic genotoxic effect in dogs.
publishDate 2017
dc.date.none.fl_str_mv 2017-03-01
2018-12-11T17:12:49Z
2018-12-11T17:12:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-736X2017000300009
Pesquisa Veterinaria Brasileira, v. 37, n. 3, p. 257-260, 2017.
1678-5150
0100-736X
http://hdl.handle.net/11449/174778
10.1590/S0100-736X2017000300009
S0100-736X2017000300257
2-s2.0-85020987737
S0100-736X2017000300257.pdf
url http://dx.doi.org/10.1590/S0100-736X2017000300009
http://hdl.handle.net/11449/174778
identifier_str_mv Pesquisa Veterinaria Brasileira, v. 37, n. 3, p. 257-260, 2017.
1678-5150
0100-736X
10.1590/S0100-736X2017000300009
S0100-736X2017000300257
2-s2.0-85020987737
S0100-736X2017000300257.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pesquisa Veterinaria Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 257-260
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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