Novel Role of VisP and the Wzz System during O-Antigen Assembly in Salmonella enterica Serovar Typhimurium Pathogenesis
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1128/IAI.00319-18 http://hdl.handle.net/11449/164447 |
Resumo: | Salmonella enterica serovars are associated with diarrhea and gastroenteritis and are a helpful model for understanding host-pathogen mechanisms. Salmonella enterica serovar Typhimurium regulates the distribution of O antigen (OAg) and presents a trimodal distribution based on Wzy polymerase and the Wzz(ST) (longchain-length OAg [L-OAg]) and Wzz(fepE) (very-long-chain-length OAg [VL-OAg]) copolymerases; however, several mechanisms regulating this process remain unclear. Here, we report that LPS modifications modulate the infectious process and that OAg chain length determination plays an essential role during infection. An increase in VL-OAg is dependent on Wzy polymerase, which is promoted by a growth condition resembling the environment of Salmonella-containing vacuoles (SCVs). The virulence- and stress-related periplasmic protein (VisP) participates in OAg synthesis, as a Delta visP mutant presents a semirough OAg phenotype. The Delta visP mutant has greatly decreased motility and J774 macrophage survival in a colitis model of infection. Interestingly, the phenotype is restored after mutation of the wzz(ST) or Wzz(fepE) gene in a Delta visP background. Loss of both the visP and wzz(ST) genes promotes an imbalance in flagellin secretion. L-OAg may function as a shield against host immune systems in the beginning of an infectious process, and VL-OAg protects bacteria during SCV maturation and facilitates intramacrophage replication. Taken together, these data highlight the roles of OAg length in generating phenotypes during S. Typhimurium pathogenesis and show the periplasmic protein VisP as a novel protein in the OAg biosynthesis pathway. |
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Novel Role of VisP and the Wzz System during O-Antigen Assembly in Salmonella enterica Serovar Typhimurium PathogenesisSalmonellaLPSO antigenVisPlipopolysaccharideSalmonella enterica serovars are associated with diarrhea and gastroenteritis and are a helpful model for understanding host-pathogen mechanisms. Salmonella enterica serovar Typhimurium regulates the distribution of O antigen (OAg) and presents a trimodal distribution based on Wzy polymerase and the Wzz(ST) (longchain-length OAg [L-OAg]) and Wzz(fepE) (very-long-chain-length OAg [VL-OAg]) copolymerases; however, several mechanisms regulating this process remain unclear. Here, we report that LPS modifications modulate the infectious process and that OAg chain length determination plays an essential role during infection. An increase in VL-OAg is dependent on Wzy polymerase, which is promoted by a growth condition resembling the environment of Salmonella-containing vacuoles (SCVs). The virulence- and stress-related periplasmic protein (VisP) participates in OAg synthesis, as a Delta visP mutant presents a semirough OAg phenotype. The Delta visP mutant has greatly decreased motility and J774 macrophage survival in a colitis model of infection. Interestingly, the phenotype is restored after mutation of the wzz(ST) or Wzz(fepE) gene in a Delta visP background. Loss of both the visP and wzz(ST) genes promotes an imbalance in flagellin secretion. L-OAg may function as a shield against host immune systems in the beginning of an infectious process, and VL-OAg protects bacteria during SCV maturation and facilitates intramacrophage replication. Taken together, these data highlight the roles of OAg length in generating phenotypes during S. Typhimurium pathogenesis and show the periplasmic protein VisP as a novel protein in the OAg biosynthesis pathway.Sao Paulo State Univ, Sch Pharmaceut Sci, Dept Biol Sci, Araraquara, SP, BrazilUniv Georgia, Coll Vet Med, Ctr Vaccines & Immunol, Dept Infect Dis, Athens, GA USASao Paulo State Univ, Sch Pharmaceut Sci, Dept Biol Sci, Araraquara, SP, BrazilAmer Soc MicrobiologyUniversidade Estadual Paulista (Unesp)Univ GeorgiaSilva, Patrick da [UNESP]Manieri, Fernanda Z. [UNESP]Herrera, Carmen M.Trent, M. StephenMoreira, Cristiano G. [UNESP]2018-11-26T17:54:35Z2018-11-26T17:54:35Z2018-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article21application/pdfhttp://dx.doi.org/10.1128/IAI.00319-18Infection And Immunity. Washington: Amer Soc Microbiology, v. 86, n. 8, 21 p., 2018.0019-9567http://hdl.handle.net/11449/16444710.1128/IAI.00319-18WOS:000439474900023WOS000439474900023.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInfection And Immunity1,954info:eu-repo/semantics/openAccess2024-06-24T13:07:51Zoai:repositorio.unesp.br:11449/164447Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:04:41.674282Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Novel Role of VisP and the Wzz System during O-Antigen Assembly in Salmonella enterica Serovar Typhimurium Pathogenesis |
title |
Novel Role of VisP and the Wzz System during O-Antigen Assembly in Salmonella enterica Serovar Typhimurium Pathogenesis |
spellingShingle |
Novel Role of VisP and the Wzz System during O-Antigen Assembly in Salmonella enterica Serovar Typhimurium Pathogenesis Silva, Patrick da [UNESP] Salmonella LPS O antigen VisP lipopolysaccharide |
title_short |
Novel Role of VisP and the Wzz System during O-Antigen Assembly in Salmonella enterica Serovar Typhimurium Pathogenesis |
title_full |
Novel Role of VisP and the Wzz System during O-Antigen Assembly in Salmonella enterica Serovar Typhimurium Pathogenesis |
title_fullStr |
Novel Role of VisP and the Wzz System during O-Antigen Assembly in Salmonella enterica Serovar Typhimurium Pathogenesis |
title_full_unstemmed |
Novel Role of VisP and the Wzz System during O-Antigen Assembly in Salmonella enterica Serovar Typhimurium Pathogenesis |
title_sort |
Novel Role of VisP and the Wzz System during O-Antigen Assembly in Salmonella enterica Serovar Typhimurium Pathogenesis |
author |
Silva, Patrick da [UNESP] |
author_facet |
Silva, Patrick da [UNESP] Manieri, Fernanda Z. [UNESP] Herrera, Carmen M. Trent, M. Stephen Moreira, Cristiano G. [UNESP] |
author_role |
author |
author2 |
Manieri, Fernanda Z. [UNESP] Herrera, Carmen M. Trent, M. Stephen Moreira, Cristiano G. [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Georgia |
dc.contributor.author.fl_str_mv |
Silva, Patrick da [UNESP] Manieri, Fernanda Z. [UNESP] Herrera, Carmen M. Trent, M. Stephen Moreira, Cristiano G. [UNESP] |
dc.subject.por.fl_str_mv |
Salmonella LPS O antigen VisP lipopolysaccharide |
topic |
Salmonella LPS O antigen VisP lipopolysaccharide |
description |
Salmonella enterica serovars are associated with diarrhea and gastroenteritis and are a helpful model for understanding host-pathogen mechanisms. Salmonella enterica serovar Typhimurium regulates the distribution of O antigen (OAg) and presents a trimodal distribution based on Wzy polymerase and the Wzz(ST) (longchain-length OAg [L-OAg]) and Wzz(fepE) (very-long-chain-length OAg [VL-OAg]) copolymerases; however, several mechanisms regulating this process remain unclear. Here, we report that LPS modifications modulate the infectious process and that OAg chain length determination plays an essential role during infection. An increase in VL-OAg is dependent on Wzy polymerase, which is promoted by a growth condition resembling the environment of Salmonella-containing vacuoles (SCVs). The virulence- and stress-related periplasmic protein (VisP) participates in OAg synthesis, as a Delta visP mutant presents a semirough OAg phenotype. The Delta visP mutant has greatly decreased motility and J774 macrophage survival in a colitis model of infection. Interestingly, the phenotype is restored after mutation of the wzz(ST) or Wzz(fepE) gene in a Delta visP background. Loss of both the visP and wzz(ST) genes promotes an imbalance in flagellin secretion. L-OAg may function as a shield against host immune systems in the beginning of an infectious process, and VL-OAg protects bacteria during SCV maturation and facilitates intramacrophage replication. Taken together, these data highlight the roles of OAg length in generating phenotypes during S. Typhimurium pathogenesis and show the periplasmic protein VisP as a novel protein in the OAg biosynthesis pathway. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:54:35Z 2018-11-26T17:54:35Z 2018-08-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1128/IAI.00319-18 Infection And Immunity. Washington: Amer Soc Microbiology, v. 86, n. 8, 21 p., 2018. 0019-9567 http://hdl.handle.net/11449/164447 10.1128/IAI.00319-18 WOS:000439474900023 WOS000439474900023.pdf |
url |
http://dx.doi.org/10.1128/IAI.00319-18 http://hdl.handle.net/11449/164447 |
identifier_str_mv |
Infection And Immunity. Washington: Amer Soc Microbiology, v. 86, n. 8, 21 p., 2018. 0019-9567 10.1128/IAI.00319-18 WOS:000439474900023 WOS000439474900023.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Infection And Immunity 1,954 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
21 application/pdf |
dc.publisher.none.fl_str_mv |
Amer Soc Microbiology |
publisher.none.fl_str_mv |
Amer Soc Microbiology |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129016499011584 |