Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralization
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/jbm.a.37076 http://hdl.handle.net/11449/200920 |
Resumo: | The present study evaluated bone marrow aspirate (BMA) and low-level laser therapy (LLLT) on bone healing. It was created critical-size defects (CSD) of 5 mm diameter in rat calvaria of 64 rats. Animals were randomly divided into four groups: Control (blood clot), BMA (coagulated BMA), LLLT (laser irradiation and blood clot), and BMA/LLLT (laser irradiation and coagulated BMA). Euthanasia was performed at 15 or 30 days postoperative. Immunohistochemical reactions were performed to identify vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA), runt-related transcription factor-2 (Runx2), bone morphogenetic protein-2 (BMP-2), osteocalcin (OCN), and osteopontin (OPN). The markers were quantified, and data were statistically analyzed. Groups BMA/LLLT and LLLT presented significantly higher VEGF expression than group control. Group BMA/LLLT presented a significantly higher expression of PCNA than all experimental groups. Groups BMA and BMA/LLLT presented significantly higher expression of BMP-2 than all experimental groups. Groups LLLT and BMA/LLLT presented significantly higher expression of OPN than groups control and BMA. Groups LLLT, BMA, and BMA/LLLT presented a significantly higher expression of OCN than group control. It can be concluded that the association of BMA and LLLT enhanced bone healing by improving expression of VEGF, PCNA, Runx2, BMP-2, OPN, and OCN. |
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Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralizationbone marrowbone regenerationimmunohistochemistrylow-level laser therapymesenchymal stem cellsThe present study evaluated bone marrow aspirate (BMA) and low-level laser therapy (LLLT) on bone healing. It was created critical-size defects (CSD) of 5 mm diameter in rat calvaria of 64 rats. Animals were randomly divided into four groups: Control (blood clot), BMA (coagulated BMA), LLLT (laser irradiation and blood clot), and BMA/LLLT (laser irradiation and coagulated BMA). Euthanasia was performed at 15 or 30 days postoperative. Immunohistochemical reactions were performed to identify vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA), runt-related transcription factor-2 (Runx2), bone morphogenetic protein-2 (BMP-2), osteocalcin (OCN), and osteopontin (OPN). The markers were quantified, and data were statistically analyzed. Groups BMA/LLLT and LLLT presented significantly higher VEGF expression than group control. Group BMA/LLLT presented a significantly higher expression of PCNA than all experimental groups. Groups BMA and BMA/LLLT presented significantly higher expression of BMP-2 than all experimental groups. Groups LLLT and BMA/LLLT presented significantly higher expression of OPN than groups control and BMA. Groups LLLT, BMA, and BMA/LLLT presented a significantly higher expression of OCN than group control. It can be concluded that the association of BMA and LLLT enhanced bone healing by improving expression of VEGF, PCNA, Runx2, BMP-2, OPN, and OCN.Dental School of Presidente Prudente Graduate Program in Dentistry (GPD-Master's Degree) UNOESTE-University of Western Sao PauloDivision of Periodontics Dental School of Araçatuba Univ. Estadual Paulista–UNESPDivision of Clinical Surgery and Animal Reproduction Veterinary School of Araçatuba Univ. Estadual Paulista–UNESPDivision of Periodontics Dental School of Pelotas Federal University of Pelotas–UFPelPeriodontics Private PracticeDivision of Periodontics School of Dentistry of Ribeirão Preto University of São Paulo-USPDivision of Histology Dental School of Araçatuba Univ. Estadual Paulista-UNESPDivision of Periodontics Dental School of Araçatuba Univ. Estadual Paulista–UNESPDivision of Clinical Surgery and Animal Reproduction Veterinary School of Araçatuba Univ. Estadual Paulista–UNESPDivision of Histology Dental School of Araçatuba Univ. Estadual Paulista-UNESPUNOESTE-University of Western Sao PauloUniversidade Estadual Paulista (Unesp)Universidade Federal de Pernambuco (UFPE)Private PracticeUniversidade de São Paulo (USP)Santinoni, Carolina S. [UNESP]Neves, Adrieli P. C. [UNESP]Almeida, Breno F. M. [UNESP]Kajimoto, Natália C. [UNESP]Pola, Natália M.Caliente, Eliana A. [UNESP]Belem, Eduarda L. G. [UNESP]Lelis, Joilson B. [UNESP]Fucini, Stephen E. [UNESP]Messora, Michel R.Garcia, Valdir G. [UNESP]Bomfim, Suely R. M. [UNESP]Ervolino, Edilson [UNESP]Nagata, Maria J. H. [UNESP]2020-12-12T02:19:36Z2020-12-12T02:19:36Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/jbm.a.37076Journal of Biomedical Materials Research - Part A.1552-49651549-3296http://hdl.handle.net/11449/20092010.1002/jbm.a.370762-s2.0-85089598211Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Biomedical Materials Research - Part Ainfo:eu-repo/semantics/openAccess2024-09-04T18:04:10Zoai:repositorio.unesp.br:11449/200920Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T18:04:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralization |
title |
Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralization |
spellingShingle |
Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralization Santinoni, Carolina S. [UNESP] bone marrow bone regeneration immunohistochemistry low-level laser therapy mesenchymal stem cells |
title_short |
Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralization |
title_full |
Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralization |
title_fullStr |
Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralization |
title_full_unstemmed |
Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralization |
title_sort |
Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralization |
author |
Santinoni, Carolina S. [UNESP] |
author_facet |
Santinoni, Carolina S. [UNESP] Neves, Adrieli P. C. [UNESP] Almeida, Breno F. M. [UNESP] Kajimoto, Natália C. [UNESP] Pola, Natália M. Caliente, Eliana A. [UNESP] Belem, Eduarda L. G. [UNESP] Lelis, Joilson B. [UNESP] Fucini, Stephen E. [UNESP] Messora, Michel R. Garcia, Valdir G. [UNESP] Bomfim, Suely R. M. [UNESP] Ervolino, Edilson [UNESP] Nagata, Maria J. H. [UNESP] |
author_role |
author |
author2 |
Neves, Adrieli P. C. [UNESP] Almeida, Breno F. M. [UNESP] Kajimoto, Natália C. [UNESP] Pola, Natália M. Caliente, Eliana A. [UNESP] Belem, Eduarda L. G. [UNESP] Lelis, Joilson B. [UNESP] Fucini, Stephen E. [UNESP] Messora, Michel R. Garcia, Valdir G. [UNESP] Bomfim, Suely R. M. [UNESP] Ervolino, Edilson [UNESP] Nagata, Maria J. H. [UNESP] |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
UNOESTE-University of Western Sao Paulo Universidade Estadual Paulista (Unesp) Universidade Federal de Pernambuco (UFPE) Private Practice Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Santinoni, Carolina S. [UNESP] Neves, Adrieli P. C. [UNESP] Almeida, Breno F. M. [UNESP] Kajimoto, Natália C. [UNESP] Pola, Natália M. Caliente, Eliana A. [UNESP] Belem, Eduarda L. G. [UNESP] Lelis, Joilson B. [UNESP] Fucini, Stephen E. [UNESP] Messora, Michel R. Garcia, Valdir G. [UNESP] Bomfim, Suely R. M. [UNESP] Ervolino, Edilson [UNESP] Nagata, Maria J. H. [UNESP] |
dc.subject.por.fl_str_mv |
bone marrow bone regeneration immunohistochemistry low-level laser therapy mesenchymal stem cells |
topic |
bone marrow bone regeneration immunohistochemistry low-level laser therapy mesenchymal stem cells |
description |
The present study evaluated bone marrow aspirate (BMA) and low-level laser therapy (LLLT) on bone healing. It was created critical-size defects (CSD) of 5 mm diameter in rat calvaria of 64 rats. Animals were randomly divided into four groups: Control (blood clot), BMA (coagulated BMA), LLLT (laser irradiation and blood clot), and BMA/LLLT (laser irradiation and coagulated BMA). Euthanasia was performed at 15 or 30 days postoperative. Immunohistochemical reactions were performed to identify vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA), runt-related transcription factor-2 (Runx2), bone morphogenetic protein-2 (BMP-2), osteocalcin (OCN), and osteopontin (OPN). The markers were quantified, and data were statistically analyzed. Groups BMA/LLLT and LLLT presented significantly higher VEGF expression than group control. Group BMA/LLLT presented a significantly higher expression of PCNA than all experimental groups. Groups BMA and BMA/LLLT presented significantly higher expression of BMP-2 than all experimental groups. Groups LLLT and BMA/LLLT presented significantly higher expression of OPN than groups control and BMA. Groups LLLT, BMA, and BMA/LLLT presented a significantly higher expression of OCN than group control. It can be concluded that the association of BMA and LLLT enhanced bone healing by improving expression of VEGF, PCNA, Runx2, BMP-2, OPN, and OCN. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:19:36Z 2020-12-12T02:19:36Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/jbm.a.37076 Journal of Biomedical Materials Research - Part A. 1552-4965 1549-3296 http://hdl.handle.net/11449/200920 10.1002/jbm.a.37076 2-s2.0-85089598211 |
url |
http://dx.doi.org/10.1002/jbm.a.37076 http://hdl.handle.net/11449/200920 |
identifier_str_mv |
Journal of Biomedical Materials Research - Part A. 1552-4965 1549-3296 10.1002/jbm.a.37076 2-s2.0-85089598211 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Biomedical Materials Research - Part A |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021401431638016 |