Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralization

Detalhes bibliográficos
Autor(a) principal: Santinoni, Carolina S. [UNESP]
Data de Publicação: 2020
Outros Autores: Neves, Adrieli P. C. [UNESP], Almeida, Breno F. M. [UNESP], Kajimoto, Natália C. [UNESP], Pola, Natália M., Caliente, Eliana A. [UNESP], Belem, Eduarda L. G. [UNESP], Lelis, Joilson B. [UNESP], Fucini, Stephen E. [UNESP], Messora, Michel R., Garcia, Valdir G. [UNESP], Bomfim, Suely R. M. [UNESP], Ervolino, Edilson [UNESP], Nagata, Maria J. H. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1002/jbm.a.37076
http://hdl.handle.net/11449/200920
Resumo: The present study evaluated bone marrow aspirate (BMA) and low-level laser therapy (LLLT) on bone healing. It was created critical-size defects (CSD) of 5 mm diameter in rat calvaria of 64 rats. Animals were randomly divided into four groups: Control (blood clot), BMA (coagulated BMA), LLLT (laser irradiation and blood clot), and BMA/LLLT (laser irradiation and coagulated BMA). Euthanasia was performed at 15 or 30 days postoperative. Immunohistochemical reactions were performed to identify vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA), runt-related transcription factor-2 (Runx2), bone morphogenetic protein-2 (BMP-2), osteocalcin (OCN), and osteopontin (OPN). The markers were quantified, and data were statistically analyzed. Groups BMA/LLLT and LLLT presented significantly higher VEGF expression than group control. Group BMA/LLLT presented a significantly higher expression of PCNA than all experimental groups. Groups BMA and BMA/LLLT presented significantly higher expression of BMP-2 than all experimental groups. Groups LLLT and BMA/LLLT presented significantly higher expression of OPN than groups control and BMA. Groups LLLT, BMA, and BMA/LLLT presented a significantly higher expression of OCN than group control. It can be concluded that the association of BMA and LLLT enhanced bone healing by improving expression of VEGF, PCNA, Runx2, BMP-2, OPN, and OCN.
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spelling Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralizationbone marrowbone regenerationimmunohistochemistrylow-level laser therapymesenchymal stem cellsThe present study evaluated bone marrow aspirate (BMA) and low-level laser therapy (LLLT) on bone healing. It was created critical-size defects (CSD) of 5 mm diameter in rat calvaria of 64 rats. Animals were randomly divided into four groups: Control (blood clot), BMA (coagulated BMA), LLLT (laser irradiation and blood clot), and BMA/LLLT (laser irradiation and coagulated BMA). Euthanasia was performed at 15 or 30 days postoperative. Immunohistochemical reactions were performed to identify vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA), runt-related transcription factor-2 (Runx2), bone morphogenetic protein-2 (BMP-2), osteocalcin (OCN), and osteopontin (OPN). The markers were quantified, and data were statistically analyzed. Groups BMA/LLLT and LLLT presented significantly higher VEGF expression than group control. Group BMA/LLLT presented a significantly higher expression of PCNA than all experimental groups. Groups BMA and BMA/LLLT presented significantly higher expression of BMP-2 than all experimental groups. Groups LLLT and BMA/LLLT presented significantly higher expression of OPN than groups control and BMA. Groups LLLT, BMA, and BMA/LLLT presented a significantly higher expression of OCN than group control. It can be concluded that the association of BMA and LLLT enhanced bone healing by improving expression of VEGF, PCNA, Runx2, BMP-2, OPN, and OCN.Dental School of Presidente Prudente Graduate Program in Dentistry (GPD-Master's Degree) UNOESTE-University of Western Sao PauloDivision of Periodontics Dental School of Araçatuba Univ. Estadual Paulista–UNESPDivision of Clinical Surgery and Animal Reproduction Veterinary School of Araçatuba Univ. Estadual Paulista–UNESPDivision of Periodontics Dental School of Pelotas Federal University of Pelotas–UFPelPeriodontics Private PracticeDivision of Periodontics School of Dentistry of Ribeirão Preto University of São Paulo-USPDivision of Histology Dental School of Araçatuba Univ. Estadual Paulista-UNESPDivision of Periodontics Dental School of Araçatuba Univ. Estadual Paulista–UNESPDivision of Clinical Surgery and Animal Reproduction Veterinary School of Araçatuba Univ. Estadual Paulista–UNESPDivision of Histology Dental School of Araçatuba Univ. Estadual Paulista-UNESPUNOESTE-University of Western Sao PauloUniversidade Estadual Paulista (Unesp)Universidade Federal de Pernambuco (UFPE)Private PracticeUniversidade de São Paulo (USP)Santinoni, Carolina S. [UNESP]Neves, Adrieli P. C. [UNESP]Almeida, Breno F. M. [UNESP]Kajimoto, Natália C. [UNESP]Pola, Natália M.Caliente, Eliana A. [UNESP]Belem, Eduarda L. G. [UNESP]Lelis, Joilson B. [UNESP]Fucini, Stephen E. [UNESP]Messora, Michel R.Garcia, Valdir G. [UNESP]Bomfim, Suely R. M. [UNESP]Ervolino, Edilson [UNESP]Nagata, Maria J. H. [UNESP]2020-12-12T02:19:36Z2020-12-12T02:19:36Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/jbm.a.37076Journal of Biomedical Materials Research - Part A.1552-49651549-3296http://hdl.handle.net/11449/20092010.1002/jbm.a.370762-s2.0-85089598211Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Biomedical Materials Research - Part Ainfo:eu-repo/semantics/openAccess2024-09-04T18:04:10Zoai:repositorio.unesp.br:11449/200920Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T18:04:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralization
title Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralization
spellingShingle Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralization
Santinoni, Carolina S. [UNESP]
bone marrow
bone regeneration
immunohistochemistry
low-level laser therapy
mesenchymal stem cells
title_short Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralization
title_full Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralization
title_fullStr Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralization
title_full_unstemmed Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralization
title_sort Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralization
author Santinoni, Carolina S. [UNESP]
author_facet Santinoni, Carolina S. [UNESP]
Neves, Adrieli P. C. [UNESP]
Almeida, Breno F. M. [UNESP]
Kajimoto, Natália C. [UNESP]
Pola, Natália M.
Caliente, Eliana A. [UNESP]
Belem, Eduarda L. G. [UNESP]
Lelis, Joilson B. [UNESP]
Fucini, Stephen E. [UNESP]
Messora, Michel R.
Garcia, Valdir G. [UNESP]
Bomfim, Suely R. M. [UNESP]
Ervolino, Edilson [UNESP]
Nagata, Maria J. H. [UNESP]
author_role author
author2 Neves, Adrieli P. C. [UNESP]
Almeida, Breno F. M. [UNESP]
Kajimoto, Natália C. [UNESP]
Pola, Natália M.
Caliente, Eliana A. [UNESP]
Belem, Eduarda L. G. [UNESP]
Lelis, Joilson B. [UNESP]
Fucini, Stephen E. [UNESP]
Messora, Michel R.
Garcia, Valdir G. [UNESP]
Bomfim, Suely R. M. [UNESP]
Ervolino, Edilson [UNESP]
Nagata, Maria J. H. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv UNOESTE-University of Western Sao Paulo
Universidade Estadual Paulista (Unesp)
Universidade Federal de Pernambuco (UFPE)
Private Practice
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Santinoni, Carolina S. [UNESP]
Neves, Adrieli P. C. [UNESP]
Almeida, Breno F. M. [UNESP]
Kajimoto, Natália C. [UNESP]
Pola, Natália M.
Caliente, Eliana A. [UNESP]
Belem, Eduarda L. G. [UNESP]
Lelis, Joilson B. [UNESP]
Fucini, Stephen E. [UNESP]
Messora, Michel R.
Garcia, Valdir G. [UNESP]
Bomfim, Suely R. M. [UNESP]
Ervolino, Edilson [UNESP]
Nagata, Maria J. H. [UNESP]
dc.subject.por.fl_str_mv bone marrow
bone regeneration
immunohistochemistry
low-level laser therapy
mesenchymal stem cells
topic bone marrow
bone regeneration
immunohistochemistry
low-level laser therapy
mesenchymal stem cells
description The present study evaluated bone marrow aspirate (BMA) and low-level laser therapy (LLLT) on bone healing. It was created critical-size defects (CSD) of 5 mm diameter in rat calvaria of 64 rats. Animals were randomly divided into four groups: Control (blood clot), BMA (coagulated BMA), LLLT (laser irradiation and blood clot), and BMA/LLLT (laser irradiation and coagulated BMA). Euthanasia was performed at 15 or 30 days postoperative. Immunohistochemical reactions were performed to identify vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA), runt-related transcription factor-2 (Runx2), bone morphogenetic protein-2 (BMP-2), osteocalcin (OCN), and osteopontin (OPN). The markers were quantified, and data were statistically analyzed. Groups BMA/LLLT and LLLT presented significantly higher VEGF expression than group control. Group BMA/LLLT presented a significantly higher expression of PCNA than all experimental groups. Groups BMA and BMA/LLLT presented significantly higher expression of BMP-2 than all experimental groups. Groups LLLT and BMA/LLLT presented significantly higher expression of OPN than groups control and BMA. Groups LLLT, BMA, and BMA/LLLT presented a significantly higher expression of OCN than group control. It can be concluded that the association of BMA and LLLT enhanced bone healing by improving expression of VEGF, PCNA, Runx2, BMP-2, OPN, and OCN.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:19:36Z
2020-12-12T02:19:36Z
2020-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1002/jbm.a.37076
Journal of Biomedical Materials Research - Part A.
1552-4965
1549-3296
http://hdl.handle.net/11449/200920
10.1002/jbm.a.37076
2-s2.0-85089598211
url http://dx.doi.org/10.1002/jbm.a.37076
http://hdl.handle.net/11449/200920
identifier_str_mv Journal of Biomedical Materials Research - Part A.
1552-4965
1549-3296
10.1002/jbm.a.37076
2-s2.0-85089598211
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Biomedical Materials Research - Part A
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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